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Citations for Bio-Rad :
351 - 400 of 1535 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Each qPCR assay was performed in 50 μl with PCR master mix (iQ™ Multiplex Powermix, Bio-Rad Life Science), 250 nM primers and 100 nM probe ...
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bioRxiv - Neuroscience 2022Quote: ... aliquots of first-stranded cDNA were amplified with gene-specific primers and Power SYBR Green PCR Master Mix (Bio-Rad) using a Step-1 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction for the multiplex real time PCRs contained 1× SYBR Green qPCR Master Mix (Bio-Rad, Hercules, CA, USA), 10 ng of each template ...
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bioRxiv - Bioengineering 2019Quote: ... Triplicate PCR reactions were performed in 20 µl final volume with 10 ng of fragmented gDNA using the ddPCR Supermix for Probes (No dUTP) master mix (Biorad) with 11-11 pmol primers and 50-50 pmol of TaqMan probes (for sequences of primers and probes see Table 1 below) ...
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bioRxiv - Molecular Biology 2020Quote: ... QRT-PCR was performed with Hieff® qPCR SYBR Green Master Mix (Yeasen, China) using CFX96 system (Bio-Rad, USA). The qRT-PCR primer sequences were available in Table 1 ...
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bioRxiv - Pathology 2020Quote: ... The reaction for the multiplex real time PCRs contained 1× SYBR Green qPCR Master Mix (Bio-Rad, Hercules, CA, USA), 10 ng of each template ...
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bioRxiv - Genomics 2021Quote: qPCR reactions were carried out in 25 µl total volume containing 5 ng of cDNA sample and 0.3 µM designed primers and SYBR Green Master Mix (Bio-Rad). Amplification was performed by Step One Plus (Applied Bio System ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was performed using the SYBR-Green PCR Master mix and amplification was run on a CFX96 thermal cycler (BioRad). The Pfaffl method was used to determine relative expression levels (Pfaffl ...
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bioRxiv - Plant Biology 2022Quote: ... The qPCR was carried out using ChamQ Universal SYBR qPCR Master Mix (Vazyme) in the CFX96 Connect Real-time PCR Detection system (BioRad). Actin2 was used as the internal control and the sequences of the primers used for RT-qPCR is provided in Supplementary Table 1.
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bioRxiv - Plant Biology 2022Quote: ... The qRT-PCR was performed with SYBR Green PCR master mix (Biotool) using the CFX96 real-time system (Bio-Rad). Gene expression was calculated by the 2−ΔΔCt method and normalized to the reference gene ACTIN ...
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bioRxiv - Plant Biology 2022Quote: ... The cDNA of the three genes were amplified by PCR using iProof HF Master Mix (Bio-Rad, Hercules, CA, USA) and cloned into the pJET1.2 vector (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... followed by performing real-time qPCR using Maxima SYBR Green/ROX qPCR master mix on a CFX96 thermocycler (Bio-Rad) with the programmes as described in genotyping the glna-knockout strain ...
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bioRxiv - Molecular Biology 2023Quote: ... Each qPCR Real-time reaction was carried out in a total volume of 10 µL with 20 ng of cDNA using SYBR-Green PCR Master Mix (BioRad) and an iCycler IQ Real-time PCR System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time RT-qPCR was performed using the AB StepOnePlus Detection System and the one-step kit RT-PCR Master Mix Reagents (#64471423, Biorad). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... followed by performing real-time qPCR using Maxima SYBR Green/ROX qPCR master mix on a CFX96 thermocycler (Bio-Rad) with the programmes as described in genotyping the sir-2.1/2.2-knockout strain ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR reactions were carried out using SYBR green Master Mix on a CFX96 Real Time system (BIO-RAD). Primer sequences are provided in the following table ...
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bioRxiv - Physiology 2023Quote: ... 500 ng RNA was reverse transcribed using HiScript III 1st Strand cDNA Synthesis Kit followed by qPCR reactions using SYBR Green master mix and analyzed by Biorad CFX Touch Real-Time PCR Detection System (SIA-PCR008) ...
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bioRxiv - Genetics 2023Quote: ... cDNA template was then used for qRT-PCR SSO Universal SYBR® Green Master Mix with ROX (BioRad, Hercules, CA) in an Applied Biosystems StepOne Plus PCR machine (Foster City ...
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bioRxiv - Microbiology 2022Quote: ... The virus genome solutions of each well were homogeneized by shaking and two 2 μL aliquots were taken for qPCR titration using the my-Budget 5x EvaGreen qPCR-Mix II (Bio-Budget) following the manufacturer’s instructions and a CFX96 Real-Time System machine (BioRad). See supplementary table 1 for primers.
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bioRxiv - Microbiology 2021Quote: ... 7 μl of 2X Laemmli buffer (BioRad) and 2 μl of β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2019Quote: ... IQ SYBR Green Supermix (2x; Bio-Rad), and 0.5μM PCR primers (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in 2X Laemmli sample buffer (Biorad) and incubated at 95°C for 5 minutes to elute the immunoprecipitated proteins ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in 2X Laemmli sample buffer (Biorad) and incubated at 95°C for 5 minutes to elute the immunoprecipitated proteins.
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bioRxiv - Bioengineering 2019Quote: ... 2x Laemmli sample buffer (Bio-Rad Laboratories), was added to supernatants and samples were boiled at 100 °C for 5 mins ...
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bioRxiv - Microbiology 2021Quote: ... 7 µl of 2X Laemmli buffer (BioRad) and 2 µl of β-mercaptoethanol ...
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bioRxiv - Physiology 2022Quote: ... mixed with 2X Laemmli buffer (Bio-Rad) and run on polyacrilamyide gels ...
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bioRxiv - Bioengineering 2023Quote: ... and 2x Tricine sample buffer (Bio-Rad) was added at a 1:1 volume to the cell lysates ...
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bioRxiv - Cell Biology 2023Quote: ... 2x Laemmli sample buffer (Bio-Rad Laboratories) was added to the lysate for a 1:1 dilution and the sample was boiled at 100 °C for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 2X Laemmli solubilizing buffer (BioRad), and stored at -20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 100µL of 2x Laemmli buffer (Bio-Rad) was added ...
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bioRxiv - Cell Biology 2024Quote: ... mixed with 2x Laemmli buffer (Bio-Rad) containing 100 mM DTT and boiled for 5 min at 95°C for western blot ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR reactions were prepared with SYBR Green RT-PCR Master mix (Thermo; S9194) and run with a CFX Connect Real-Time System (Bio-Rad). Samples were run in triplicate ...
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bioRxiv - Microbiology 2020Quote: ... The quantitative realtime PCR was performed with SYBR Green qPCR master mix (Vazyme, Q311-02) and data were analyzed by Bio-Rad CFX96 system ...
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bioRxiv - Evolutionary Biology 2022Quote: ... ONSEN copy numbers were determined by qPCR using 12 ng total DNA using the KAPA SYBR FAST master mix universal on a C1000 Touch (Bio-Rad) machine ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μL of the sample was used as a template for qRT-PCR analysis with SYBR Select Master Mix on a CFX96 thermal cycler (Bio-Rad). The relative expression levels of the selected target genes were determined using the ΔΔCt method and normalized to the geometric mean of the ACTB and TBP mRNA levels using the primers listed in Table S2.
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bioRxiv - Neuroscience 2019Quote: ... and analyzed using qPCR as described previously (Jankowski et al., 2009) using optimized primers and SsoAdvanced SYBR Green Master Mix (BIO-RAD). Threshold cycle time (Ct ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative PCR was performed with 1 μg of cDNA per sample per target gene using AceQ qPCR SYBR Green master mix (Vazyme) on a CFX 96 Touch real-time PCR system (Bio-Rad). For YAP-TEAD inhibition studies ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples for RT-qPCR were prepared in a final volume of 10 µl and analyzed using Applied Biosystems’s SYBR Green PCR master mix (Bio-Rad Laboratories) during 40 cycles of amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... ∼500bp fragments of sRNA factors were amplified from cDNA of ISE6 cells using primers listed in Table S1 using iProof High- Fidelity Master Mix (Bio-Rad) and the amplicons were treated with XhoI to insert them in the XhoI site of pLitmus28i (NEB) ...
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bioRxiv - Immunology 2022Quote: ... The abundance of target gene expression was measured by qRT-PCR using a Bio-Rad CFX96 real-time PCR apparatus and a SYBR Green Master Mix (Bio-Rad) for all PCR reactions ...
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bioRxiv - Neuroscience 2022Quote: ... 5ng of cDNA was loaded per qRT-PCR reaction using fast SYBR master mix and reactions run on a CFX96 RT-PCR detection system (Bio-Rad). The following primers were used:
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bioRxiv - Bioengineering 2022Quote: ... The qRT-PCR was carried out using a SYBR Green Realtime PCR Master Mix (TOYOBO) in a CFX Connect Real-Time PCR Detection System (Bio-Rad). The forward and reverse primers used for qRT-PCR are provided in table S2.
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were run using the ChamQ SYBR Color qPCR Master Mix (Vazyme Biotech, China) on Bio-RAD CFX384 real-time system (BioRad, USA). The enrichment values were normalized to the input sample ...
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bioRxiv - Cancer Biology 2021Quote: qPCR reactions were performed using PrimeTime Gene Expression Master Mix (Integrated DNA Technologies 1055770) and CFX384 touch real-time PCR detection system (Bio-Rad). The reaction were comprised of 200 ng gDNA ...
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bioRxiv - Genetics 2022Quote: ... cDNA concentration was quantified by qPCR per the published protocol except using SsoAdvanced Universal SYBR Green qPCR master mix (Biorad 1725274) and following manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: ... Hieff qPCR SYBR Green Master Mix (Yeasen Biotechnology, shanghai) were used for signal generation detected by CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Relative expression levels were calculated as transcript levels of target genes relative to housekeeping gene GAPDH ...
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bioRxiv - Physiology 2022Quote: ... The PCR cycle number for each library amplification was optimized by running 10% of the library DNA in a real-time PCR reaction using Brilliant III Ultra-fast SYBR Green QPCR Master Mix (AH Diagnostic) and a C1000 Thermal cycler (Bio-Rad). DNA libraries were sequenced on a HiSeq2000 by 50□bp single□end sequencing at the National High Throughput Sequencing Centre (University of Copenhagen ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR analyses were performed using ChamQ SYBR qPCR Master Mix (Vazyme Biotech, China) on a CFX384 Touch (Bio-Rad, USA). The PCR conditions were as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... The converted DNA was eluted in DNase/RNase-free water and examined by PCR using SYBR Green PCR Master Mix (Bio-Rad) and primers listed in Supplementary Table S2 ...
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bioRxiv - Cancer Biology 2021Quote: ... qPCR was performed on Bio-Rad CFX96 Touch Real-Time PCR Detection System using SYBR® Green Master Mix (Bio-Rad) following manufacturer’s protocol.