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Citations for Bio-Rad :
451 - 500 of 1535 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Real-Time qPCR was performed using KAPA SYBR® FAST qPCR Master Mix (KAPABiosystems) on the CFX Connect Real-time PCR system (Bio-rad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR in triplicate for each sample was performed using 2xTSINGKE® Master qPCR Mix (SYBR Green I) (TSINGKE) on the CFX96 Real-Time PCR system (Bio-Rad). The expression of β-Actin was used as the internal control.
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bioRxiv - Plant Biology 2023Quote: ... was performed utilizing ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX; TQ ID1110, SMOBIO, Taiwan) with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, USA). The expression of the PcActin gene was normalized to elongation factor-1a of N ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 ng of genomic DNA per sample was added to duplicate PCR reactions using the ddPCR™ Supermix for Probes (No dUTP) master mix (Bio-Rad). Droplets were prepared and measured according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was performed using the Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Q712-02) on Bio-Rad CFX-96 Touch Real-time PCR System (Bio-Rad, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed using BlitzAmp Hotstart qPCR Master Mix (Mirexes, 1204201) with CFX96 Real-Time System (BIO-RAD, Hercules, CA, USA) as per manufacturers’ protocols ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed in triplicate with the BioRad C1000 Touch™ Thermal Cycler and CFX384™ Real-Time System using SYBR Green PCR Master Mix (BioRad) together with sequence specific primers ...
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bioRxiv - Genetics 2021Quote: ... and 2x Laemmli Sample Buffer (Bio-Rad, #1610737) was added prior to gel loading ...
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bioRxiv - Cancer Biology 2022Quote: ... A loading buffer made from 2x Laemlli (Biorad) was prepared with the instructed amount of beta-mercaptoethanol and added to the protein samples for a final concentration of 2 µg/µL ...
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bioRxiv - Physiology 2019Quote: ... and Prime PCR Preamp Assays 2X (Bio-Rad), listed in Supplementary Table 1 ...
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bioRxiv - Bioengineering 2020Quote: ... 2x TBE-urea sample loading buffer (BIO-RAD) was added to each reaction mixture ...
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bioRxiv - Microbiology 2020Quote: ... mixed with 2X Laemmli Sample Buffer (Bio-Rad) and 2-mercaptoethanol (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... containing 10 μL 2X EvaGreen Supermix (Bio-Rad), 1 μL 4 μM forward primer ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 μl 2x Laemmli Sample Buffer (Bio-Rad) with 5% b-mercaptoethanol were added to the sample followed by incubation at 95°C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Lysates mixed with 2x Lammeli Sample Buffer (BioRad) supplemented with beta-mercaptoethanol were boiled for 5 min and separated on 12% SDS-PAGE gels prior to transfer to methanol-activated PVDF membranes using the Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... 2x iQ SYBR SUPERMix (Bio-Rad, REF: 1708880) with Read 1 (5’-TATGGTAATT GT GTGYCAGCMGCCGCGGTAA-3’) ...
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bioRxiv - Plant Biology 2023Quote: ... resuspended in 2x Laemmli protein sample buffer (BIORAD), boiled ...
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bioRxiv - Cell Biology 2023Quote: ... re-suspended in 2X Laemmli Buffer (Bio-Rad), boiled for 10min at 95°C and used for WB as above.
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bioRxiv - Cell Biology 2023Quote: ... boiled in 50 μl 2x sample buffer (BioRad), and separated from the eluted IP sample using a magnet ...
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bioRxiv - Cell Biology 2023Quote: ... and eluted in 2x Laemmli buffer (Bio-Rad). Proteins were separated by SDS-PAGE on a 4-20% gradient mini-PROTEAN® Precast Gel (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... One volume 2X Laemmli solution (Bio-rad, 1610737) supplemented with 1.3 mM ß-mercaptoethanol (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 µL 2x SDS Laemmli buffer (1610747, BioRad) supplemented with 5% 2-betamercaptaethanol (M3148 ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... Remaining protein was eluted using 2X SDS (Biorad) sample buffer with 100 mM DTT (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... qPCR experiments were conducted using a Roche LightCycler 480 II and Roche LightCycler 480 SYBR Green I Master SYBR-Green (Bio-Rad, Hercules, CA). Primers were designed to detect the KAN-MX resistance gene located on plasmids and genomic TUB1 (control ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed on 96-well optical reaction plates with one-step SYBR Green PCR master mix (Bio-Rad Laboratories, Cat#1725150).
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bioRxiv - Immunology 2021Quote: ... DNA contamination in the treated RNA samples was assessed by performing a qPCR cycling with tilapia elongation factor 1α (EF-1α) primers using No-RT master mix (absence of reverse transcriptase enzyme provided in iScript™ Reverse Transcription kit, Bio-Rad, USA). The cDNA synthesis (20 µL reactions ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed using Hieff qPCR SYBR® Green Master Mix (11201ES08, Yeasen, Shanghai, China) on a CFX96 quantitative PCR system (Bio-Rad, USA). Data were processed using CFX Maestro 1.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA corresponding to approximately 10 ng of starting RNA was used and qPCR was performed with SYBR green master mix (Bio-Rad Laboratories, Inc.). Primers for real-time PCR were GAPDH-F ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using the Taq Pro Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China) reaction system on the CFX96 Real-Time detection system (Bio-Rad, CA, USA). Each experiment was performed with three technical replicates and the UBQ10 was used as the endogenous control for data analysis.
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bioRxiv - Molecular Biology 2020Quote: ... or TaqMan Gene Expression Master Mix (mRNA qPCR) per the manufacturer’s protocol on a BioRad CFX96 Touch Real Time PCR Detection System (Bio-Rad Laboratories, Richmond, CA). Reactions were performed in triplicate using either U6 (miRNA qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... Relative qRT-PCR was performed in a total volume of 25 µl with 12.5 µl SYBER GREEN master mix (1725121, BioRad Laboratories, Hercules, CA, USA), 1µl gene-specific primer mix ...
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bioRxiv - Pathology 2020Quote: ... The qRT-PCR analysis was performed in triplicate with ChamQ™ SYBR® qPCR Master Mix (Without ROX) using the CFX96 Real-Time PCR Detection System (Bio-Rad), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time PCR analysis of target mRNA-expression levels was achieved using SYBR Green qPCR Master Mix (Bio-Rad, Hercules, CA, USA) and the CFX Connect Real-time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of purified RNAs from the control and treated tissues were reverse-transcribed and amplified using Brilliant SYBR Green QRT-PCR Master Mix (Bio-Rad, Hercules, CA). Human β-actin was used as an internal control ...
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bioRxiv - Genomics 2022Quote: ... or TaqMan Gene Expression Master Mix (mRNA qPCR) per the manufacturer’s protocol on a BioRad CFX96 Touch Real Time PCR Detection System (Bio-Rad Laboratories, Richmond, CA). Reactions were performed in duplicate or triplicate using either U6 (miRNA qPCR ...
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bioRxiv - Genetics 2023Quote: ... SYBR qPCR Master Mix kit (Q711, Vazyme, Nanjing, China) and a Bio-Rad CFX96 real-time PCR detection system (Bio-Rad, Hercules, USA) were used for qRT-PCR ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was diluted 10 fold in nuclease free water and reactions were performed in a Bio-Rad CFX96 system using gene specific primers (Table S1) and Bio-Rad SYBR green master mix (Bio-Rad, Hercules, USA). Each sample was run in triplicate ...
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bioRxiv - Cell Biology 2024Quote: ... 4 µl of the diluted cDNA was added to a Master mix containing 4 µl SsoAdvanced Universal SYBR® Green Supermix (Bio-rad #1725270) and 0.5 µl of a 10 µM stock of each Forward and Reverse primer (Table 5 ...
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bioRxiv - Molecular Biology 2023Quote: ... was performed by analyzing 10 ng gDNA (in triplicate) in 10 µL total volume of SYBR green PCR master mix (Bio-Rad, Hercules, CA) using the Bio-Rad real-time system using 400 nM of EGFP-specific primers (Supplementary Table S4) ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was mixed with Hieff® qPCR SYBR Green Master Mix (Yeasen Biotechnology, 11201ES08) and subjected to qPCR with a sequence detection system (CFX; Bio-Rad Laboratories). The qPCR primers used in this study are described in Supplementary Table S3.
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bioRxiv - Microbiology 2023Quote: ... 1 µL primer 556R (5’ CTTTACGCCCARTRAWTCCG 3’) at 10 µM and 10 µL SYBER® Green Master Mix (Bio-Rad, Veenendaal, The Netherlands). The DNA extraction estimated an average rRNA yield corresponding to 5×10^8 bacterial cells/mL.
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bioRxiv - Cell Biology 2020Quote: ... An equal volume of 2x Laemmli buffer (BIO-RAD) with β-mercaptoethanol was added to the cell lysate and denaturing by heating at 97 °C ...
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bioRxiv - Immunology 2021Quote: ... Beads were boiled with 2x Sample buffer (Bio-Rad) with β-ME for 5 minutes at 95 degrees ...
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bioRxiv - Biochemistry 2022Quote: ... 50 μL 2x Laemmli Sample Buffer (Biorad, 161-0737) with 2-Mercaptoethanol (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were resuspended in 2x laemmli buffer (Bio-Rad) with 5% 2-Mercaptoethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in 20ul of 2X LDS loading buffer (BioRad), and 80% of each sample was loaded per well on 15 wells 4-15% Mini Protean TGX gels (BioRad ...
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bioRxiv - Biochemistry 2019Quote: ... Equal volumes of 2X SDS loading buffer (Bio-Rad) containing 10% β-mercaptoethanol were added and the samples were boiled at 95 oC for 10 min and allowed to cool to room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... QX200 EvaGreen 2x Supermix was used (BioRad, cat. #1864034) with 0.5 μm of primers and appropriate amounts of cDNA ...
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bioRxiv - Neuroscience 2020Quote: ... using the 2x iQ SYBR Green Supermix (Bio-Rad). Ct for each gene was determined after normalization to GAPDH or RPLP0 and ΔΔCt was calculated relative to the designated reference sample ...
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bioRxiv - Cell Biology 2019Quote: ... and solubilized in 2x Laemmli sample buffer (Bio-rad). All timecourses were performed at ∼25°C ...