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Citations for Bio-Rad :
551 - 600 of 1066 citations for 7 Bromo benzo c isothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... before being transferred to Whatman 3 MM paper and dried (50°C, 2 hours) using a gel dryer (Model 583, BioRad) attached to a vacuum pump ...
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bioRxiv - Developmental Biology 2022Quote: Cells in monolayer were fixed in 4% PFA for 20 minutes at 4°C and blocked for 30 minutes in 10% donkey serum (BioRad) and 0,1% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 h at 4 °C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged for 15 min at 10,000 x g at 4 °C and supernatants were collected for protein quantification using the Quick start Bradford protein assay kit 2 (Biorad). Samples were mixed with Laemmli buffer (Biorad ...
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bioRxiv - Biochemistry 2022Quote: ... for 20 min at 4 °C) protein concentrations in supernatants collected were determined using a Bradford protein assay (Bio-Rad). Remaining supernatants were mixed with sample buffer at 3:1 v /v ratio ...
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bioRxiv - Microbiology 2022Quote: ... centrifuged for 20 minutes at 4°C and supernatants mixed 3:1 with 4x Laemmli sample buffer (Bio-rad 1610747). Samples were heated at 95°C for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were eluted by heating the beads at 95°C for 5 min in Laemmli Sample Buffer (1610747, Bio-Rad) with 50 mM DTT followed by magnetic separation ...
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bioRxiv - Neuroscience 2020Quote: ... 0.02% bromphenol blue) for 15min at 50°C and 5μg of protein were separated on a 4-20% SDS PAGE (Biorad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Developmental Biology 2020Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Molecular Biology 2019Quote: ... Plates were incubated overnight at 30 °C and imaged the following day using a Gel Doc EZ Gel Documentation System (BioRad) and Image Lab (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed using SYBR Green (Applied Biosystem – 4309155) and primers listed in Table S1 at an annealing temperature of 60°C on a CFX96 thermal cycler (Biorad). Generated data were analysed using the CFX Manager Software (Biorad).
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bioRxiv - Microbiology 2020Quote: ... it was activated in an oven at 120°C and imaged using a gel doc XR image system (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... centrifuged for 20 minutes at 13,000 rpm at 4°C and the protein concentration in the supernatant was measured with an DC protein assay (BioRad). Samples were separated by SDS-PAGE ...
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bioRxiv - Neuroscience 2022Quote: ... heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (4 –15% Tris-HCl; BioRad) and transferred onto Immun-Blot PVDF 0.2 µm (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were then denatured in 2X Laemmli at 95°C for 5 minutes and run in Mini-PROTEAN Precast Gels (BioRad) and transferred onto membranes using Trans-Blot Turbo ...
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bioRxiv - Biophysics 2019Quote: ... Reactions were incubated at 30°C for 60 minutes and then analyzed by denaturing TBE-Urea PAGE with fluorescence detection (ChemiDoc MP, BioRad).
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bioRxiv - Developmental Biology 2019Quote: ... Homogenates were centrifuged (11,000 rpm, 10 min, 4°C) and resultant supernatant assayed using a DC Protein Assay Kit (Bio-Rad). A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were heated at 95°C for 5 min and separated on a 12% Bis-Tris SDS-PAGE gel (Biorad).
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bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
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bioRxiv - Microbiology 2019Quote: ... The drips were allowed to dry and the plates were incubated at 37°C for 16 h and imaged using the fluorescein setting on a ChemiDoc MP Imaging machine (Biorad).
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bioRxiv - Microbiology 2020Quote: ... The samples were heated at 90°C for 10 minutes and loaded onto a 4-20% precast polyacrylamide gel (BioRad). The gel was stained with SimplyBlue SafeStain (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Purified TREK-2 was then mixed with GUVs to a final concentration of ∼1 - 5 µg/ml and incubated overnight at 4 °C with 0.5mg/ml Bio-Beads (Bio-Rad) prior to use.
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bioRxiv - Genomics 2019Quote: ... at 4 °C and transferred to a nitrocellulose membrane (0.45 μM) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current of 0.12 A for 1 □ hour ...
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bioRxiv - Microbiology 2021Quote: ... for 60 min at 37°C followed by purification on a Micro-Bio Spin P-6 Gel Column (Bio-Rad). 1 pmol of 5’-end-radiolabeled primer was used for each extension reaction ...
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bioRxiv - Genetics 2019Quote: ... chromosomal DNA was prepared in agarose plugs as described in (Bani Ismail et al. 2014) and run at 14 °C in a CHEF DR-III apparatus (BioRad) using the field 6V/cm at a 120° angle ...
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bioRxiv - Genetics 2019Quote: ... Protein samples were denatured at 95°C for 5 min in appropriate loading buffer and loaded into precast SDS-PAGE gels (10% polyacrylamide; Biorad). Gels were run at 40 mA for 45 min in 1x running buffer (Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... containing 2μL of 20X reducing buffer at 95°C for 10 min and separated by SDS-PAGE short migration on 12% SDS-PAGE (Criterion XT Precast gels–BioRad). After a short migration ...
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min at 100°C in SDS-PAGE sample buffer and separated on Tris-glycine 4-20% SDS-PAGE gels (BioRad). Detected protein bands for each blot were quantified using ImageJ software for gel densitometry measurements of NSP4:GAPDH.
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bioRxiv - Microbiology 2019Quote: ... The lysate was used to bind to 200ul of IgG Sepharose beads (Amersham Biosciences) for 2h at 4°C using a disposable chromatography column (BioRad). The IgG-Sepharose column was washed with 35 ml of protein A binding buffer (10 mM Tris–HCl ...
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bioRxiv - Physiology 2019Quote: ... Protein lysates were prepared by centrifugation at 15,000g for 10min at 4°C and protein concentrations were calculated using Bradford assay (BioRad, UK) with BSA as a standard ...
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bioRxiv - Cell Biology 2020Quote: ... The embryos were mounted on a 2 % agarose pad and heat-shocked at 30°C for 1h (Thermocycler Bio-Rad). After 2h recovery at 20°C ...
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad). PVDF membranes were used for proteins wet transfer (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation at 10,000xg for 10 minutes at 4°C and then boiled in 2x Laemmli Sample Buffer (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Samples (10 µg protein) were denatured at 95°C for 5 min in 4x Laemmli Sample Buffer (1610747, Bio-Rad) or Novex NuPAGE LDS Sample Buffer (NP0007 ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared for 10 min at 14000 rpm at 4°C and protein concentration of the supernatant was determined by using Protein Assay (BioRad). The co-immunoprecipitation was performed using GFP trap® beads (Chromotek ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction mixture was incubated for 30 minutes at 30°C and stopped by adding 12 μl of Laemmli sample buffer (Biorad). After HMT reactions ...
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bioRxiv - Microbiology 2021Quote: ... for 10 minutes at 37 °C and analyzed with Bio-Rad S3 Cell Sorter (Bio-Rad Laboratories Inc., California, USA) and ProSort™ Software using green fluorescence as a trigger.
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bioRxiv - Immunology 2021Quote: Steady-state equilibrium binding of hCoV-2IG lots was monitored at 25°C using a ProteOn surface plasmon resonance (BioRad). The purified recombinant SARS-CoV-2 RBD proteins were captured to a Ni-NTA sensor chip with 200 resonance units (RU ...
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bioRxiv - Microbiology 2020Quote: ... coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 14,000 rcf for 20 min at 4°C and protein amounts in the supernatant determined using the Bradford assay (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was subsequently washed 3 times in TBS+0.05 % Tween-20 over 45 min and incubated with secondary antibodies for 1 h at RT (20 °C) after washing 3 times in TBS+0.05 %Tween-20 and developed by enhanced chemiluminescence (Bio-Rad) with Image Quant™ LAS 4000.
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bioRxiv - Neuroscience 2021Quote: ... and equal protein amounts were boiled at 95°C for 10 minutes prior to being run on a gel (BioRad) at 120V ...
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bioRxiv - Microbiology 2022Quote: ... as counterstain to denote stained areas and incubated at 37°C in a humidified chamber according to the manufacturer’s instructions (Bio-Rad kit 357-2112 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad, no. 4568083) in running buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Electrophoresis was performed for 21 h at 14°C in 0.9% (wt/vol) Pulse Field Certified Agarose (Bio-Rad Laboratories) in exactly 2.4L of 0.5x Tris-borate/EDTA buffer in a Rotaphor apparatus (Biometra ...
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bioRxiv - Molecular Biology 2022Quote: ... The mixtures were incubated for 20 min at 37°C and loaded onto a Bio-Dot Microfiltration Apparatus (Bio-Rad) loaded with nitrocellulose (0.2 μm Amersham Protran ...