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Citations for Bio-Rad :
4801 - 4850 of 4927 citations for Rat 3 Hydroxy 3 Methylglutaryl CoA Reductase HMGCR ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of total RNA was used as the template to synthesize cDNA using the iScript RT-PCR kit (Bio-Rad, Carlsbad, CA). qPCR was performed with the SYBR Green I nucleic acid gel stain (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cervicornis sperm samples from 2015 were brought to a final concentration of 3 x 107 cells ml-1 after the addition of Cell Suspension Buffer and 2% agarose using the Bio-Rad CHEF Genomic DNA Plug Kits (Bio-Rad, Hercules, CA). Genomic DNA plugs were processed according to the manufacturer’s protocol and stored at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... one-step reverse transcription droplet digital PCR (RT-ddPCR) assays were performed with the One-step RT-ddPCR Advanced Kit for probes (Bio-Rad Laboratories Ltd.), using primers and probes targeting 2’,5’-oligoadenylate synthetase 1 (OAS1) ...
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bioRxiv - Molecular Biology 2024Quote: The synthesis of cDNA was done from 100 ng RNA in 20 μl total reaction volume using iScript Reverse Transcription Supermix kit (Cat# 1708841, Bio-Rad, Hercules, USA), without any changes from themanufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... A total of 1000 ng of RNA was used for cDNA synthesis with the High-Capacity cDNA Reverse Transcription Kit (Bio-Rad, Hercules, CA). Real-time PCR was performed with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: Total protein lysates were prepared from the cells followed by protein quantification using the protein DC assay kit (Bio-Rad, Cat # 5000-001) as described previously (28) ...
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bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
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bioRxiv - Microbiology 2022Quote: ... and TNF-α was analyzed by using quantitative real-time PCR according to the Biorad iScript One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers (F/R) ...
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bioRxiv - Cancer Biology 2023Quote: PDEC cytokine secretion was analyzed from cleared PDEC culture supernatants using Bio-Plex Pro Human Cytokine 27-plex assay kit (Bio-Rad, cat. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and PCR amplification were performed in a single reaction using the One-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, CA, USA) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration in cell lysates were measured using a RC DC Protein Assay Kit (cat. no. 50000119; Bio-Rad Laboratories, Hercules, CA, USA). Immunoblotting was carried out using standard procedures ...
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bioRxiv - Physiology 2023Quote: One hundred nanograms of total RNA was reverse-transcribed (RT) and amplified using the iScript One-Step RT-PCR kit for probes (Bio-Rad, Hercules, CA). Real-time qPCR was performed with the Bio-Rad CFX96 sequence detection system using predesigned primer/probe sets against CPT1a ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA was extracted from a liver of an adult female ferret using the CHEF Mammalian Genomic DNA Plug Kit (BioRad, Cat. No. #1703591). In brief ...
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bioRxiv - Cancer Biology 2023Quote: ... The resultant homogeneous single-cell suspensions were embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit). Plugs were allowed to solidify at 4°C and were then incubated with Proteinase K (QIAGEN ...
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bioRxiv - Immunology 2023Quote: ... Equal concentrations of RNA (measured by NanoDrop and 1 µg for the caecum tissue) were reverse transcribed to cDNA using the iScript™ cDNA Synthesis Kit (Bio-Rad, #1708891) and stored at −20°C ...
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bioRxiv - Cell Biology 2022Quote: ... The purified RNA was quantified by NanoDrop and 1 µg of RNA for each sample was used to generate cDNA using the iScript cDNA synthesis kit (Biorad cat. no. 1708890). Real-time PCR was performed with PowerUp SYBR Green Master Mix (Applied Biosystems cat ...
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bioRxiv - Cancer Biology 2023Quote: Messenger RNA expression was assessed with 150 to 500 ng of total RNA using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, USA, #1708891) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... SYBR qPCR Master Mix kit (Q711, Vazyme, Nanjing, China) and a Bio-Rad CFX96 real-time PCR detection system (Bio-Rad, Hercules, USA) were used for qRT-PCR ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cDNA synthesis was performed for 57 samples using 500 ng of RNA per sample and the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Inc.). qPCR primers for the abovementioned genes were designed as described in Ahi & Sefc ...
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bioRxiv - Immunology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, United States). The limit of detection was calculated to be 10 copies per reaction.
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bioRxiv - Immunology 2023Quote: ... Cytokine levels in cell culture supernatants was determined using the Magnetic Luminex Performance Assay (Human Base Kit A; R&D Systems, coupled with the Bio-Plex 200 (Bio-Rad). The trimmed median value was used to derive the standard curve and calculate sample concentrations.
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bioRxiv - Immunology 2023Quote: ... The supernatant was transferred to a new microcentrifuge tube and protein concentration was determined by using a DC protein assay kit (BioRad, Cat# 500-0116). Cell lysates (500-1000 μg ...
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bioRxiv - Biochemistry 2023Quote: ... total RNA (1 µg) was taken from the cDNA preparation using iScript™ cDNA Synthesis Kit (#1708891, Bio-Rad Laboratories, Hercules, CA, USA). Prior to cDNA synthesis ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolation of total RNA was performed using the Aurum™ Total RNA Mini-Kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). Total RNA concentration and quality were determined using a Nanodrop 1000 (ThermoFisher) ...
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bioRxiv - Systems Biology 2023Quote: ... Japan) and quantitative PCR using a KOD SYBR qPCR kit (TOYOBO) with CFX96 Real-Time PCR System (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... The preparation of the genomic DNA plugs was carried out according to the CHEF genomic DNA plug kit (Bio-Rad, Hercules, CA, USA). The DNA templates of sgRNA-actF and sgRNA-actR were generated by overlap extension of sgRNA-actF/sgRNA-actR and guide RNA-F plus guide RNA-R ...
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bioRxiv - Neuroscience 2023Quote: ... We performed the reverse transcription (RT) reaction with 200 ng/µl of total RNA with the iScript gDNA clear advanced kit (Bio-Rad 172-5035) and the real time qPCR was performed with the Sso Advanced SYBR Green® Supermix (Bio-Rad 172-5270 ...
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bioRxiv - Microbiology 2023Quote: ... The fungal burden of each fungus ball was determined by measuring the galactomannan (GM) content in the supernatant [43] using the Platelia Aspergillus enzyme immunoassay kit (Bio-Rad, Hercules, CA) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... 1 µg RNA was synthesized to cDNA by reverse-transcription using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA; item # 1708891). Real-time PCR was performed in 10 µl reactions containing 5 µl SsoFast EvaGreen Supermix with Low ROX (Bio-Rad ...
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bioRxiv - Pathology 2024Quote: ... Quantitative PCR was performed to quantify Lrp2 mRNA abundance in the kidney using SsoFast™ EvaGreen® Supermix kits (Cat # 172-5204; Bio-Rad) on a Bio-Rad CFX96 cycler ...
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bioRxiv - Immunology 2024Quote: Cytokine secretion was analyzed from cleared PDEC culture supernatants using Bio-Plex Pro Human Cytokine 27-plex assay kit (Bio-Rad, cat. M500KCAF0Y) and Bio-Plex 200 System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... followed by real-time RT-PCR targeting highly conserved regions of the IAV matrix (M) (10) using iTaq Universal Probes One-Step Kit (Bio-Rad, 1725141, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The anti-HBs concentrations of the DBS samples were then tested using the Monolisa Anti-HBs PLUS kit (Bio-Rad, Hercules, CA, USA) with a 2.00 mIU/mL lower limit of detection (LoD ...
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bioRxiv - Immunology 2024Quote: ... and RNA was isolated by column purification (ZymoResearch R2050) and cDNA was synthesized using the iScript Reverse Transcriptase kit (Bio-Rad, Hercules, CA). qPCR was performed using SYBR green PCR master mix (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: Reverse transcription for qPCR was either performed using gene-specific reverse priming with the iScript™ Select cDNA Synthesis Kit (Bio-Rad #1708897) or using NEB LunaScript RT SuperMix kit (NEB #E3010L) ...
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bioRxiv - Microbiology 2024Quote: ... Non-diluted supernatant of tissue homogenate (50 μl) was applied to Bio-plex pro mouse cytokine & chemokine assay kit (Bio-Rad, #M60-009RDPD).
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bioRxiv - Cell Biology 2020Quote: ... RNA template (1 μg) was prepared and reverse transcription reaction was performed with iScript Advanced cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA) according to the procedure described in the product’s instruction manual ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted from SSLs using 350 μL of RIPA buffer and was purified using the Ready Prep 2-D cleanup kit (Bio-Rad, Hercules, CA, USA); the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... Total RNA (410 ng) was reverse transcribed into cDNA using an iScript™ Synthesis Kit on a T100™ thermal cycler (BIO-RAD, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Target proteins were detected by chemiluminescence with Clarity™ (Beta-actin and GFP) and Clarity Max™ (SP) Western ECL Substrate kit (Bio-Rad®) on the Odyssey FC system (LI-COR) ...
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bioRxiv - Cancer Biology 2020Quote: ... One μg RNA was reverse-transcribed using oligo(dT)18 and iScript Advanced cDNA synthesis kit for RT-qPCR (Bio-Rad Laboratories; Hercules, CA). Il1r1/IL1R1 and Gapdh/GAPDH qPCR was performed using specific primers and Lightcycler 480 Sybr Green I Master (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... the cDNA was synthesised using the Bio-Rad iScript™ cDNA Synthesis Kit (25 x 20 µl reactions #1708890, Bio-Rad Laboratories Inc., USA), while the qPCR reaction was performed using the Applied Biosystems PowerUp™ SYBR™ Green Master Mix (A25741 ...
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bioRxiv - Microbiology 2021Quote: ... The amount of viral RNA expressed as TCID50 equivalent per insert (TCID50e/insert) was quantified by RT-qPCR using iTaq universal probes one-step kit (Bio-Rad, catalogue no. 1725141), and a commercial mix of primers for N gene (forward primer 5’-GACCCCAAAATCAGCGAAAT-3’ ...
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bioRxiv - Neuroscience 2022Quote: ... and cDNAs were synthesized from 200 ng of RNA of each sample using iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad, Hercules, CA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Chemiluminescence reaction was initiated with Western Bright Quantum Western blotting detection kit and measured in a ChemiDocTM imaging system (BIO-RAD, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... and their CWD status was independently identified utilizing the Bio-Rad TeSeE Short Assay Protocol (SAP) Combo Kit (BioRad Laboratories Inc., Hercules, CA, USA). Positive RPLNs were confirmed by IHC at the Colorado State University Veterinary Diagnostic Laboratory) ...
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bioRxiv - Microbiology 2020Quote: ... IL-22 and TNF-α was analyzed by using quantitative RT-PCR (qRT-PCR) according to the Biorad iScript™ One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... or mRNA isolated from cell lines or primary mouse cells were proceeded to RT-qPCR using iTaq universal SYBR green one step kit (Bio-Rad, Cat. No. 1725150). DNA obtained from ChIP was proceed to RT-qPCR using iQ SYBR green supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was reverse transcribed into cDNA via iScriptTM Reverse Transcription Supermix and real-time quantitative PCR was performed with SsoAdvancedTM Universal SYBR® Green Supermix in a CFX96 Touch™ thermocycler (all kits and devices from Bio-Rad Laboratories) using 10 ng of cDNA as input ...