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Citations for Bio-Rad :
4401 - 4450 of 4927 citations for Rat 3 Hydroxy 3 Methylglutaryl CoA Reductase HMGCR ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... 1 μg of RNA was reverse transcribed (RT) using the iScript Reverse Transcription Supermix for RT-PCR kit (#170-8840 Bio-Rad), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of total RNA from all samples were transcribed into cDNA using iScript cDNA Synthesis Kits (Bio-Rad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized in a 10-μl volume containing 350 ng of plant total RNA that was reverse transcribed employing an iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The extracted RNA was transcribed into cDNA according to a standard protocol using iScript™ cDNA Synthesis Kit (BIO-RAD, USA) for sequencing analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... 500ng of RNA from clinical samples were converted to cDNA using the iScript cDNA synthesis kit from Biorad (Catalog number: 1708891). Primers that overlapped intron-exon junction were designed using the NCI Primer-blast software and are listed in Supplementary Table 13 ...
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bioRxiv - Pathology 2020Quote: ... 1 μg of total RNA was used as a template for reverse transcription assay using the iScript cDNA Synthesis Kit (Bio-Rad); cDNA was used for detecting FAM210A and GAPDH expression ...
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bioRxiv - Cell Biology 2021Quote: ... The peroxidase-conjugated secondary antibody was incubated and reacted using ECL kits (Cytiva).The signal was detected using ChemiDoc XRS + (Bio-Rad). The membrane was stripped using stripping buffer (strong ...
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bioRxiv - Microbiology 2019Quote: ... First-strand cDNA for gene expression analysis using real-time qPCR was carried out using the iScript cDNA synthesis kit from Bio-Rad following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 250 ng of RNA was used as a template in each reaction with iTaq Universal One-Step RT-qPCR Kit (Bio-Rad) or Luna Universal One-Step RT-qPCR Kit (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2020Quote: RNA extraction of positive samples of the GFP fluorescence assay as well as butanediol fermentation processes was performed using the Aurum Total RNA Mini Kit (BioRad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Reverse transcription: 200 ng of RNA was reverse transcribed using iScript Advanced cDNA Synthesis kit for RT-qPCR (1702537, Bio-Rad) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were resolved on SDS polyacrylamide gels and blotted onto PVDF membranes using Trans-Blot Turbo RTA Midi 0.45 μm LF PVDF Transfer Kit (Bio-Rad, 1704275). Transfer was run in Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... the membrane was then developed by incubation with chemiluminescence (Amersham ECL prime kit) and imaged with a ChemiDoc™ XRS+ System (BIORAD). The intensity of GFP bands were quantified with Image J software ...
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bioRxiv - Bioengineering 2021Quote: ... Two-step qPCR was performed to quantify GFP gene silencing using the total RNA extracted from leaves: an iScript cDNA synthesis kit (Bio-Rad) was used to reverse transcribe total RNA into cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Austria).52 The luciferase activity data was normalised to protein content measured by DC Protein determination kit (Bio-Rad Laboratories, Inc., USA) and SCO effect was expressed as a fold-change over untreated cells ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK).
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bioRxiv - Immunology 2020Quote: ... the signals were detected using an enhanced chemiluminescence detection kit (Super Signal West Pico Plus, 34580, ThermoFischer Scientific) and membranes imaged with ChemiDoc Imaging System (Bio-Rad). The same anti-S1 and anti-S2 antibodies as well secondary antibodies were also used to detect the Spike protein on EVs by ELISA (see protocol below).
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bioRxiv - Molecular Biology 2020Quote: 500ng of genomic RNA were used for reverse transcription into cDNA using the iScript cDNA synthesis kit (Bio-Rad, #170-8891). cDNA was diluted 10X and 2uL was added for each qPCR reaction (iQ™ SYBR Green Supermix ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence substrate was then applied to the blot according to the manufacturer’s instructions (Bio-Rad Clarity™ Western ECL Substrate kit). The chemiluminescent signals were recorded using a Syngene G:BOX (Synoptics ...
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bioRxiv - Cancer Biology 2019Quote: Plasma activities of ALT and AST were assessed with the ALT and AST Reagent Kit (Biosystems Reagents) using a Benchmark Plus microplate spectrophotometer (Bio-Rad). Plasma concentration of non-sulfated bile acids were measured with the Bile Acid Assay Kit (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... ATAC Tagmentation Buffer and ATAC Tagmentation Enzyme (ATAC Tagmentation Buffer and Enzyme are both included in the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad)) ...
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bioRxiv - Genomics 2021Quote: For a detailed description of tagmentation protocols and buffer formulations refer to the SureCell ATAC-Seq Library Prep Kit User Guide (17004620, Bio-Rad). Harvested cells and tagmentation related buffers were chilled on ice ...
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bioRxiv - Genomics 2021Quote: ... One µg of RNA was then used to synthesize cDNA using the Bio-RAD iScript cDNA Synthesis Kit (Bio-RAD, 1708840). qPCR reactions for targeted genes were performed with the Luminaris HiGreen qPCR Master Mix (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... total 600 ng of RNA was used for cDNA synthesis by using Random hexamer oligonucleotide primer (iScript Select cDNA Synthesis Kit, BioRad, USA). Gene specific primers (Table S2 ...
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bioRxiv - Genomics 2020Quote: ... The concentrations of cytokines in plasma were measured using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit immunoassay run on the Bio-Plex 200 (BioRad), with reference to an 8 point standard curve.
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bioRxiv - Microbiology 2021Quote: ... First-strand cDNAs were synthesized from 500 ng DNase-treated total RNA by RT with the iScript cDNA synthesis kit (Bio-Rad) in a total volume of 20 µL according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Serum was also collected for cytokine analyses which were performed by the University of Pennsylvania’s Human Immunology Core using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane by semi-dry transfer using the Trans-Blot turbo RTA Mini PVDF transfer kit (Bio-Rad) and the Trans-Blot turbo system ...
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bioRxiv - Physiology 2021Quote: ... was performed with the Bio-Rad iQ TM SYBR Green Supermix kit and data were analyzed with the CFX384 system (Bio-Rad). mRNA quantitation was done by normalizing the amount of RNA detected for the gene of interest ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the iTaq Univer SYBR Green 1-Step Kit (Bio-Rad) on a StepOnePlus Real-time PCR system (Applied BioSystems ...
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bioRxiv - Developmental Biology 2021Quote: ... eluted total RNA in 10 μl of Elution buffer and reverse transcribed RNA in 20 μl using the iScript cDNA synthesis kit (Bio-Rad). We incubated the samples for 5 min at 25°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA (1μg) of each postnatal stage was used for cDNA synthesis using a reverse transcription kit (iscript, Bio-Rad, USA). Real-time PCR amplification was performed using iTaq™ Universal SYBR® Green Supermix (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... The antigen-antibody complexes were visualized using appropriate HRP conjugated secondary antibodies and enhanced chemiluminescence kit (ECL; Amersham or Bio-Rad) and normalization was reported to β-actin ...
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bioRxiv - Physiology 2021Quote: ... 5 ul of each supernatant sample was used to quantify the total amount of protein with a BCA Protein Assay Kit whereas the rest was diluted in 4x Laemmli sample buffer (Bio-Rad) with 2-Mercaptoethanol (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... markers was analyzed by qPCR as previously described (Yanai et al., 2013; Yanai et al., 2015) using an iScript cDNA synthesis kit (Bio-Rad) and TaqMan Primer/probe sets for each gene ...
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bioRxiv - Immunology 2020Quote: ... with 18S (Hs99999901_s1) as housekeeping control were carried out on RNA reverse transcribed to cDNA with an iScript cDNA synthesis kit (Bio-Rad), using Taqman chemistry and gene-specific probes purchased from Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: Total RNAs were extracted from cell-sorter purified cells using NucleoSpin RNA kit with a DNase treatment (Macherey Nagel) and reverse transcribed with iScript™ Reverse Transcriptase mix (Biorad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription and real-time PCR (qPCR) reactions were carried out using iScript cDNA synthesis kit and iQ SYBR Green Supermix (Bio-Rad). qPCR was performed on a Bio-Rad CFX384 Touch™ Real-Time PCR Detection System using the following human primers (Forward and Reverse ...
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bioRxiv - Immunology 2021Quote: Plasma from each patient sample was analyzed using the Bio-Plex Pro™ Human Cytokine 17-plex Assay kit (Bio-Rad). Cytokine expression was measured using the Luminex xMAP® (Luminex Corporation ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality and concentration of the RNA was assessed and first strand cDNA synthesised from 1 ug using the iScript Select cDNA Synthesis Kit (Bio-Rad) and a 1:1 mixture of Oligo (dT ...
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bioRxiv - Microbiology 2021Quote: ... The protein contents of 100-µl cell samples were measured using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). Uptake rates were calculated from retained activity and total added activity and expressed in pmol amino acid µg-1 of protein.
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bioRxiv - Microbiology 2021Quote: RNA copies of SARS-CoV-2 samples were detected by quantitative real-time RT-PCR (RT-qPCR) assays were performed using the iTaq SYBR Green One-Step Kit (Bio-Rad) on the LightCycler 480 system (Roche ...
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bioRxiv - Immunology 2021Quote: ... Sera were collected for cytokines analyses which were performed by the Macau University of Science and Technology using a Non-Human Primate Cytokine Panel kit (MilliporeSigma, Cat# PCYTMG-40K-PX23) on a Bio-Plex 200 instrument (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... According to kit instruction 10 μl reaction system was prepared and q-PCR was operated using CFX96 Real-Time System (BIO-RAD). For data analysis ...
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bioRxiv - Genetics 2020Quote: ... Equal amounts of DNA-free total RNA were converted to cDNA using the iScript™ cDNA synthesis kit (Bio-Rad #1708891). Quantitative PCR (qPCR ...
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bioRxiv - Bioengineering 2020Quote: ... anti-CD63 and anti-CD81 detection antibodies (supplied in the MACSPlex kit, 5 µl each) or anti-decoy receptor antibodies (AlexaFluor647-labelled anti-human TNFR1, Bio-Rad, cat #MCA1340A647 ...
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bioRxiv - Microbiology 2020Quote: ... the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad). The results were analyzed using Bio-Rad CFX Manager software ...
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bioRxiv - Neuroscience 2021Quote: ... adult testes or hippocampus or cell pellets were homogenized in RIPA buffer and the protein concentration was determined using a Bradford Assay kit (Bio-Rad). For blot analysis ...
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bioRxiv - Neuroscience 2022Quote: RNA isolation for all tissues were carried out using the Aurum Total RNA isolation kit (BioRad #732-6820; Hercules, CA, USA). Samples were reverse-transcribed to cDNA using qScript cDNA Supermix (Quantabio #101414-106 ...