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Citations for Bio-Rad :
4701 - 4750 of 4927 citations for Rat 3 Hydroxy 3 Methylglutaryl CoA Reductase HMGCR ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Oligo-dT first strand cDNA synthesis was performed from 5 μg total RNA with iScript Advance cDNA Synthesis Kit for RT-qPCR (Bio-Rad; #1725038) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was then washed in 6x 5 minutes of TBST prior to being developed with Clarity™ Western ECL Substrate kit (Bio-Rad). To examine actin levels as a loading control blot was stripped using Restore™ WesternBlot Stripping Buffer (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Germany) and 1 μg of total mRNA was translated to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA) according to the instructions.
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bioRxiv - Neuroscience 2022Quote: ... 500 ng of total RNA was used in a 20 µL reaction volume with the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, #1708890) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein measurements were performed using a BCA kit (Pierce) and 150 µg was loaded on a 10-20 % tris/tricine SDS-page gels (Bio-Rad Laboratories) and blotted onto 0,2 µm nitrocellulose membrane (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using gDNA of purified viruses was performed in parallel of each experiment ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription of the RNA was performed to synthesize cDNA following the standard manufacturer’s instructions of the iScript Reverse Transcription Supermix for RT-qPCR kit (Bio-Rad PN 1708841). cDNA was submitted to the University of North Carolina Chapel Hill Advanced Analytics Core (UNCAAC ...
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bioRxiv - Neuroscience 2024Quote: ... The signals were detected using an enhanced chemiluminescent (ECL) plus reagent kit (Share-Bio, Cat. SB-WB012L, China) and viewed with a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
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bioRxiv - Zoology 2024Quote: ... and 100 ng of RNA were used for cDNA synthesis with the iScriptTM gDNA Clear cDNA Synthesis Kit (Bio-Rad Laboratories, USA), resulting in a final concentration of 5 ng/μl for each sample.
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bioRxiv - Microbiology 2023Quote: ... the fluorescence derived from the incorporation of SybrGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoAdvanced Universal SYBR Green Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Maestro software ...
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bioRxiv - Bioengineering 2024Quote: Four samples of total RNA were collected for each treatment condition using the Aurum Total RNA Mini Kit (Bio-Rad, Hercules, CA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of RNA was taken to synthesize complementary DNA (cDNA) conversion reactions were done using an iScript™ Advanced cDNA Synthesis Kit (catalog no. 18080085, Biorad, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories) and the TaqMan primers/probe mixtures (all from Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: cDNA was synthesized by reverse transcription from 500 ng of total RNA according to manufacturer’s instructions (iScript™ cDNA Synthesis Kit, Bio-Rad Laboratories). Real-time PCR reactions using primers hybridizing with ERG25 or ACT1 transcripts were performed in an CFX96TM Real-Time System C100TM Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... the fluorescence of the EvaGreen dye incorporated into the PCR product was measured at the end of each cycle using SoFast EvaGreen Supermix 2× kit (Bio-Rad, France). A standard curve using the gDNA of purified viruses was performed in parallel with each experiment ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was conducted using HiScript II One Step qRT-PCR SYBR Green Kit (Q221-01, Vazyme) with gene-specific primer pairs on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories). The thermal cycling protocol was as follow ...
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bioRxiv - Bioengineering 2024Quote: RNA was diluted in nuclease-free water and combined with the reagents from the One Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, USA) and eGFP primer/probe set with final primer concentrations of 900 nM and probe concentrations of 250 nM to measure transgene expression ...
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bioRxiv - Molecular Biology 2023Quote: ... where absolute quantification was done by the QX200 Droplet Digital PCR System in combination with the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, USA). The detection limit was calculated to be 1000 copies per reaction ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged 10 minutes at 13,000 x g and the supernatant was quantified using a DC assay kit (Bio-Rad, Hercules, California). 200 µg of total protein were diluted into a total of 1.5 mL Array Buffer and rocked over the pre-blocked array membrane containing various total RTK antibodies overnight at 4°C ...
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bioRxiv - Pathology 2023Quote: ... and the other one was subjected to the western blot assay using the Trans-Blot Turbo RTA Midi 0.45 µm LF PVDF Transfer Kit (Bio-Rad Laboratories, CA). After transferring the total proteins to the PVDF membrane ...
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bioRxiv - Microbiology 2023Quote: ... for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad, USA) and the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Neuroscience 2023Quote: ... Once ready for testing we quantified the amount of mitochondria from each isolation using Bio-Rad Protein Assay Kit (Bio-Rad, #5000001), plated 3µg/50µl/well onto a 96 well plate with all animals having 6-8 technical replicates (101085-004 ...
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bioRxiv - Physiology 2023Quote: ... The cDNA was obtained from equal amounts of total RNA by reverse transcription using an iScriptTM cDNA Synthesis Kit (Bio-Rad, CA). Gene expression of 96 fibrosis-associated genes were evaluated using a PCR array kit (RT² Profiler™ PCR Array Rabbit Fibrosis PANZ-120Z ...
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bioRxiv - Plant Biology 2023Quote: ... The 1st strand cDNA was synthesised from 1 µg of RNA for each sample using iScript™ cDNA Synthesis Kit (Bio-Rad). This cDNA was then used as the template in qRT-PCR reaction mixture after 10 times dilution ...
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bioRxiv - Microbiology 2023Quote: ... modified from previously published primers/probe (44)) was performed using the BioRad iTaq Universal Probes One-Step Kit (BioRad, Cat. No. 1725140). Thermocycling was performed on a BioRad CFX Connect Real-Time PCR Detection System (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA (cDNA) synthesis was performed using 1 μg of total RNA with the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). The genes encoding for the zonula occludens-1 (Zo-1) ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were assayed through using Bio-Plex Pro-Mouse Group I Cytokine 23-plex assay kit (M60009RDPD, Bio-Rad, Hercules, California, USA) and the Bio-Plex® 200 System ...
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bioRxiv - Biochemistry 2023Quote: ... The determination of casein concentration by the Lowry method was performed using the DC™ protein assay kit purchased from Bio-Rad Laboratories (California ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized with 1 µg of total RNA by using the iScript cDNA Synthesis Kit (Bio-Rad, Cat# 1708891). cDNA was diluted 20 times and real-time quantitative PCR was performed by using iTaq Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Visualization was performed using a horseradish peroxidase sensitive ECL western blotting detection kit (SuperSignal West Pico PLUS Chemiluminescent Substrate, ThermoScientific) and the result was recorded using Chemidoc Imaging System (Bio-Rad Laboratories).
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bioRxiv - Plant Biology 2023Quote: ... Membranes were developed using the SuperSignal West Pico Chemiluminescent Substrate kit (Fisher #PI34578) and visualized on a ChemiDoc™ Touch Gel Imaging System (BioRad #1708370).
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bioRxiv - Bioengineering 2023Quote: ... We then added 80 μL of X1 streptavidin phycoerythrin SA-PE (Bio-Plex Pro Reagent Kit III #171304090M, BioRad, Hercules, CA, USA) and incubated it for another 20 minutes ...
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bioRxiv - Physiology 2023Quote: ... One hundred nanograms of RNA was used as input to synthesize cDNA using the iScript cDNA synthesis kit (BioRad, Hercules, CA, USA). cDNA templates were used for quantitative real-time PCR ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 4°C at 20,160 x g for 10 minutes and supernatants were collected for protein measurement using the DC Protein Assay Kit (Bio-Rad, 5000111). Equal amounts of protein were loaded to run the gels (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Heart homogenates were analyzed for cytokine and chemokine levels using a Bio-Plex Pro Mouse Cytokine 23-plex Assay kit (Bio-Rad, #m60009rdpd) and ProcartaPlex mouse IFN-α/IFN-β 2-plex (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified with the RC DC Protein assay Kit II (cat. No. 5000122; Bio-Rad Labortaories Inc, California, USA). After protein concentration assessment ...
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bioRxiv - Genetics 2023Quote: ... Whole cell extracts were sonicated and cleared by centrifugation and protein concentration estimated using the Bio-Rad DC protein assay kit (Bio-Rad Laboratories).
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bioRxiv - Bioengineering 2023Quote: ... After RNA isolation the respective complementary DNA (cDNA) was then synthesized using the Bio-Rad iScript cDNA synthesis kit (Bio-Rad, USA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... First-strand cDNA was synthesized from 200 ng of total RNA in a 20 μl reaction using the iScript™ cDNA Synthesis Kit (Cat#: 1708891, Bio-Rad). A SYBR green real-time PCR kit (Cat# 001752A ...
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bioRxiv - Pathology 2023Quote: ... Two μg of total RNA from each sample were used for cDNA synthesis using the iScript Advanced cDNA Synthesis kit (Bio-Rad, CA).
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bioRxiv - Genetics 2023Quote: ... and luciferase activities were normalized to total protein levels in the WCEs extracts determined using the Bradford assay kit (Bio-Rad Laboratories).
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bioRxiv - Immunology 2023Quote: ... Proteins in the gels were transferred to the PVDF membrane using the Trans-Blot Turbo Kit by the Trans-Blot Turbo transfer system (Bio-Rad, 1704156). Membranes were then incubated in a blocking buffer (Nacalai Tesque ...
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bioRxiv - Immunology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).
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bioRxiv - Microbiology 2024Quote: qRT-PCR analysis was performed on three major selected genes and validated as follows: The first cDNA strand was prepared using the iScript™ cDNA synthesis kit (Bio-Rad). qRT-PCR was performed using the SsoFast™ EvaGreen® supermix kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... CD45-APC and Bu1-PerCP-Cy5.5 were generated by conjugation of the antibody with the respective Lynx Rapid conjugation kit (Bio-Rad, Feldkirchen, Germany). Samples were analyzed on a FACS Canto II and FlowJo 10.9 software (BD ...
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bioRxiv - Microbiology 2024Quote: ... The anti-HBs concentrations of the DBS samples were tested using the Monolisa Anti-HBs PLUS kit (Bio-Rad, Hercules, CA, USA) with a 2.00 mIU/mL lower limit of detection (LoD ...
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bioRxiv - Developmental Biology 2024Quote: ... using cDNA generated from 0.1-2 ng total RNA with the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad catalog # 1864021) according to the manufacturer’s protocol with reverse transcription at 42°C and annealing/extension at 60°C.
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using the iScript™ cDNA Synthesis Kit according to the manufacturer’s instructions (Cat#1708890, Bio-Rad Laboratories, Hercules, CA). Quantitative real-time PCR was conducted using SYBR Green probes and measured using the QuantStudio using the following PCR settings ...
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bioRxiv - Microbiology 2024Quote: ... In-hospital HIV testing was done on all patients by the clinical teams using point-of care Genie™ HIV1&2 test kits (BioRad, USA).