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Citations for Bio-Rad :
401 - 450 of 6495 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Membranes were then washed 3 times in PBS-T followed by a final wash in PBS before signal development in Clarity Western ECL substrate (Bio-rad laboratories) and visualization on a ChemiDoc MP imager.
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bioRxiv - Pathology 2019Quote: ... Blots were then washed five times with TBS-T and signals were visualized by using enhanced chemiluminescence (SuperSignal West Pico, Pierce) and detected using ChemiDoc™ Imaging Systems (Bio-rad).
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bioRxiv - Bioengineering 2021Quote: ... The resulting gene fragment was ligated with the plasmid backbone using a ligase-free ligation method developed in the lab that includes incubation with 10 mM MgCl2 in a thermocycling step in a PCR thermocycler (Biorad, T-100). The cycling programme was as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed 3x with TBS-T and incubated with either Goat Anti-Mouse IgG (H+L)-HRP Conjugate (Bio-Rad, #1721011) or Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... except that blocking was done with phosphate-buffered saline plus 0.1% Triton X-100 (PBS-T) containing 5% nonfat dry milk (Biorad Blotting grade Blocker) and 10 mM NaN3 ...
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bioRxiv - Developmental Biology 2021Quote: ... Following 4x 10 min washes with PBS-T the membrane was incubated for 1 min with Clarity Western ECL Substrate (170-5060, Bio-Rad Laboratories) and imaged with Amersham™ Imager 600 (GE ...
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bioRxiv - Physiology 2021Quote: ... the membranes were rinsed three times in TBS-T for 5 min and the proteins were visualized by enhanced chemiluminescense (BioRad, Clarity 1705061) in a BioRad Universal III Hood with Image Lab software v.6.0.1 ...
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bioRxiv - Biochemistry 2023Quote: ... Each membrane was then incubated with the appropriate HRP-conjugated secondary antibodies in TBS-T and visualized by using the Enhanced chemiluminescence substrate (BioRad 170-5060) and ChemiDoc (BioRad).
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bioRxiv - Zoology 2023Quote: ... quantitative R T–PCR was performed with a Bio-Rad CFX96™ Real-time PCR Detection System (Bio-Rad, Hercules, CA, USA) according to standard protocols and cycling conditions ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:8000 in PBT for 1 h at 37 °C before the ELISA was read out using ABTS and H2O2 at an iMark Microplate Absorbance Reader (Bio-Rad Laboratories, Inc., CA, USA). ACPA IgG levels were quantified based on an in-house standard of pooled RA patient plasma using the Microplate manager software MPM-6 (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from infected cells at various time intervals after infection using Total RNA mini kit (Biorad). For RT-qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were cleared with centrifugation and protein concentrations were measured with Protein Assay kit (Bio-Rad, #5000001). 25-50 μg protein were loaded to 10-well SDS-PAGE gradient gel (Bio-Rad 456-1094) ...
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bioRxiv - Microbiology 2019Quote: RNA was extracted from infected cells at various time intervals after infection using Total RNA mini kit (Biorad). For RT-qPCR ...
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bioRxiv - Cancer Biology 2022Quote: An Aurum RNA mini kit was used to purify total RNA from the cells (Bio-Rad, Hercules, CA). For real time PCR experiments ...
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bioRxiv - Genomics 2019Quote: ... Single-cell ATAC-seq libraries were prepared using the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad) and SureCell ddSEQ Index Kit (12009360 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5×106 cells per plug: plugs have been treated with Proteinase K (CHEF genomic DNA plug kit, Biorad) and restriction-digested by BamHI (NEB ...
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bioRxiv - Genomics 2021Quote: ... Single-cell ATAC-seq libraries were prepared using the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad) and SureCell ddSEQ Index Kit (12009360 ...
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bioRxiv - Cell Biology 2021Quote: Cells were collected and their RNA was extracted using Aurum Total RNA Mini Kit (Bio-Rad; 732-6820). Total RNA concentration and purity was determined using a nanodrop spectrophotometer (Thermo Scientific) ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: The phosphorylation status of Akt was evaluated in lysed macrophage samples (Bio-Plex Cell Lysis Kit; Bio-Rad) using the phospho-Akt(Thr308 ...
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bioRxiv - Genomics 2022Quote: ... PBMCs were spun and resuspended in Cell Suspension Buffer and embedded in 0.6% agarose (plug lysis kit, BioRad). The agarose plugs were treated with Puregene Proteinase K (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Total protein content of cell free BALF was determined using the DC Protein Assay Kit (Cat 5000111; BioRAD, Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... Single-cell ATAC-seq libraries were prepared using the SureCell ATAC-Seq Library Prep Kit (17004620, Bio-Rad) and SureCell ddSEQ Index Kit (12009360 ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from infected cells at various time intervals after infection using Total RNA mini kit (Biorad). RNA present in in vitro transcription reactions was directly subjected to reverse transcription without purification ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA from cells was extracted using the Aurum™ Total RNA Mini Kit (Bio-Rad, CA, USA), and 100ng of RNA was used with iTaq™ Universal SYBR® Green One-Step Kit (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 1◻h incubation at 4◻°C with HRP-conjugated goat anti-mouse (1:5000 dilution in 1% YE in PBS-T, BioRad #170–6516) secondary antibody ...
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bioRxiv - Genetics 2021Quote: ... membranes were incubated 2h in TBS-T with 2% BSA containing secondary anti-rabbit antibodies-HRP conjugate (1:2000, #1721019 Bio-Rad, Hercules, CA). After three more washes in TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were washed five times in TBS-T before detection with chemiluminescent HRP substrate (Immobilon, P90720) and imaged on a ChemiDoc™ XRS+ system (Bio-Rad, 1708265).
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bioRxiv - Cancer Biology 2021Quote: ... NRF2 (CST, 12721S), KEAP1 (CST, 8047S), PRDX1 (CST, 8499S), GSTO1 (Thermo, PA5-83383)] in 5% BSA in TBS-T (Bio-Rad, 170-6435). Following incubation with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Then membranes were washed 3 times with TBS-T and incubated with goat anti-mouse or anti-rabbit IgG HRP-conjugated secondary antibody (1:10000 in 5% BSA TBS-T, Bio-rad; #1706515, #1706516) for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were washed again three times with TBS-T followed by the addition of primary antibodies (neat antivenom and normal horse IgG [BioRad] as negative control) in duplicate ...
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bioRxiv - Immunology 2024Quote: ... the fractions were eluted with 0.25 M NaCl and biologically active fractions were further analyzed on a hydrophobic interaction column (Macro-Prep® t-Butyl HIC Resin, Bio-Rad 1580090). Three fractions eluted with buffers with pH=7 ...
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bioRxiv - Plant Biology 2024Quote: ... the membrane was washed twice with the 1x TBS-T buffer and then incubated with the Clarity Western ECL Substrate solution (Bio-Rad, California, USA). Signal detection of the membranes was performed by the Chemi-Doc Imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes underwent three times with PBS-T and were then subjected to incubation with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, CA, USA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were quantified for protein concentrations using the Bio-Rad DC™ Protein Assay kit (Cat.5000112, BioRad) and loaded to a 10% SDS-PAGE gel ...
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bioRxiv - Bioengineering 2021Quote: ... The cells were then used to isolate the total RNA using the Aurum Total RNA Mini Kit (Biorad, #7326820) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... signals were obtained with peroxidase-conjugated secondary antibodies (Cell Signalling Technology) and revealed with Clarity Western ECL kit (Biorad) and a ChemiDoc ™ XRS Imaging system (BioRad).
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bioRxiv - Immunology 2019Quote: ... Cells were then stained for inflammasome activation using a caspase-1 FAM-YVAD-FMK FLICA probe kit (Bio-Rad). Cells were then processed for analysis by flow cytometry.
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bioRxiv - Neuroscience 2023Quote: Cortical and hippocampal brain tissue sections were lysed using the Bio-Plex cell lysis kit (Bio-Rad Laboratories #171304011) and protein concentrations were determined using a Pierce BCA Protein Assay (Thermo Fisher #23225) ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was extracted and purified from RPE tissue punches using Single Shot Cell Lysis Kit (Bio-Rad Laboratories). cDNA synthesis was performed from 1.5 μg RNA using RT² First Strand Kits (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... and cells were lysed in 350 μl/well lysis buffer from the AurumTM total RNA Mini Kit (Biorad, 7326820). The lysates were immediately frozen at −80 °C until RNA extraction ...
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bioRxiv - Microbiology 2023Quote: ... was collected from each sample and transferred to 96-well ELISA plates for the measurement of the absorbance at 595 nm by using the iMarkTM Microplate Absorbance Reader (Bio-Rad, Alfred Nobel Drive. Hercules, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... On-bead ELISA readouts were measured using the standard PE and APC detection filter parameters on a ZE5 flow cytometer (Bio-Rad; 405, 488, 561, and 640 lasers) in the Scripps Research Flow Cytometry Core Facility ...
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bioRxiv - Systems Biology 2023Quote: ... resolved in a 4-12% Invitrogen B-T gel using MOPS buffer and transferred onto a polyvinylidene difluoride (PVDF) membrane using BioRad transblot (Bio-Rad, Hercules, CA, USA). The membrane was blocked with 5% skim milk ...
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bioRxiv - Biochemistry 2023Quote: ... Then the membrane was incubated with mouse monoclonal antibodies against PAR for 60 min in PBS-T/5% milk followed by incubation with the secondary anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad, Hercules, CA, USA) for 60 min in the PBS-T/5% milk solution ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed and normalised for protein concentration using the Bio-Rad Protein Assay Kit (Bio-Rad Laboratories, Inc. # 5000001) and a spectrophotometer ...
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bioRxiv - Cancer Biology 2021Quote: Small pieces of dissected frozen pancreatic tumor tissues were collected and lysed (Bio-Plex Cell Lysis Kit, BioRad, USA; 171304011), followed by a repeated procedure of vortexing ...
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bioRxiv - Microbiology 2021Quote: After the protein concentration of cell lysates and homogenates had been determined using the DC Protein Assay Kit (Bio-Rad) 20 µg of each sample were dissolved in NuPAGE LDS Sample buffer (including 2% β-mercaptoethanol) ...
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bioRxiv - Immunology 2020Quote: Thymi isolated from Foxp3hCD2 x Rag1GFP reporter mice were homogenized and lysed using Bio-Plex Cell Lysis Kit (BioRad Laboratories), and the protein content of the thymic lysates was measured using Pierce™ BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: 1 μg of total RNA purified from differentiated cells was reversed transcribed using the One-step SuperScript kit (Bio-Rad). The qRT-PCR reaction was performed in a CFX96 Real-time PCR detection system (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: Total RNA was extracted from kidney and cells using Aurum TM Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad) or RNAqueous™ Micro Total RNA Isolation Kit (Thermo Fisher Scientific ...