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Citations for Bio-Rad :
201 - 250 of 6495 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 0.1% Tween-20 (TBS-T): Goat anti-mouse Starbright700 (1:10000, Bio-Rad), anti-rabbit rhodamine-conjugated tubulin (1:10000 ...
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bioRxiv - Microbiology 2022Quote: ... PCR reactions were amplified in a BioRad T-100 Thermocycler (Bio-Rad Laboratories) with initial denaturation at 94°C for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed in PBS-T (PBS buffer with Tween-20 (Bio-Rad) to a total concentration of 0.05%).
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bioRxiv - Molecular Biology 2023Quote: ... washed again in TBS-T and proteins visualised with ECL (Biorad, 170-5061) and an Image Quant 800 (Amersham).
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bioRxiv - Physiology 2024Quote: ... Primary antibodies were diluted in TBS-T containing 5% dry nonfat milk (Biorad). Membranes were incubated overnight at 4 °C with primary antibody ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with 0.4 µg/mL rabbit -anti-human CD20 polyclonal antibodies (Neomarkers) and 2 µg/mL rat-anti-human CD3 antibodies (clone MCA1477, BioRad). Then ...
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bioRxiv - Immunology 2020Quote: ... Living single CD45+CD3-CD14+ and/or CD11b+ macrophages (based on DAPI and scatter properties) were sorted into eight 384 wells plates (Biorad) where cDNA synthesis was performed as previously described [23].
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bioRxiv - Physiology 2019Quote: ... Real time polymerase chain reaction (RT-PCR) was performed with the CFX96 or CFX384 Touch™ Real-Time detection system (Bio-Rad Laboratories), using a SensiMix™ (BioLine ...
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bioRxiv - Physiology 2019Quote: ... Transcript levels were analyzed by real-time polymerase chain reaction (RT-PCR) (fluorescence detection of SYBR green) in an iCycler iQ apparatus (Bio-Rad, Hercules, CA), with normalization against 18S as an endogenous control ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with specific primers (Supplementary Table 2) using the iTaq™ Universal SYBR® Green Supermix (Bio-Rad) and CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse Transcription – Quantitative Polymerase Chain Reaction (RT-qPCR) reactions were performed using SsoAdvanced™ universal SYBR® green (Bio-Rad, Hercules, CA, USA) and run on the CFX Connect (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real time polymerase chain reaction for all targets was performed on the CFX-96 Real Time System using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA). Products were confirmed by gel electrophoresis ...
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bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: For the separation of myosin heavy chain isoforms a large format SDS-PAGE setup with integrated cooling core was used (Protean II xi, Bio-Rad Laboratories, Inc.). A 5% stacking gel ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Molecular Biology 2023Quote: Relative genetic expression was determined using Real Time Quantitative Polymerase Chain Reaction (RT-qPCR) (CFX96™ Real-Time System, Bio-Rad, CA, USA) using commercially available TaqMan primers (Tables 1-3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Quantitative real time polymerase chain reaction (qPCR) was performed using the iQ™ SYBR® Green Supermix (170888, Bio-Rad, Hercules, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Viral RNA genome was detected and quantified by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) on a BioRad real-time CFX96 detection system (BioRad, Hercules, United States). The target sequence for amplification was the viral RNA-dependent RNA polymerase [26].
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bioRxiv - Microbiology 2024Quote: ... We optimized the recommended polymerase chain reactions (PCR) cycling conditions and performed a touchdown PCR in a T100 thermocycler (Bio-Rad, Hercules, CA). The total reaction volume was 50 µl which contained 2 µl of genomic DNA at 50 ± 25 ng/µl ...
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bioRxiv - Systems Biology 2024Quote: ... the Bio-Plex Pro Cell Signaling Reagent Kit (BIO-RAD) was used ...
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bioRxiv - Microbiology 2024Quote: ... Lysis was conducted utilizing the SingleShot Cell Lysis Kit (BioRad), with its defined protocol ...
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bioRxiv - Immunology 2022Quote: ... Optical density (OD) was immediately read at 450 nm using an ELISA plate reader (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad] ...
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bioRxiv - Bioengineering 2021Quote: ... the respective genes were cloned through colony PCR in the thermocycler (Biorad, T-100). Specifically ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were diluted in PBS-T containing 5% blotting-grade blocker (Bio-Rad, #1706404). Membranes were incubated overnight (≈16 h) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Tween-20 (TBS-T): Goat anti-mouse Starbright700 (1:10000, Bio-Rad,12004158), Goat anti-rabbit IRDye800 (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... were removed from the eluted samples post-purification and quantified using quantitative Real Time polymerase chain reaction using the Bio-Rad CFX 96™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA) and Promega Plexor®HY Quantitation System (Promega Corporation ...
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bioRxiv - Microbiology 2024Quote: ... The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR) assays on a T100™ Thermal Cycler (Bio-Rad, Hercules, CA). Reaction volumes were 25 µL and consisted of 12.5 µL of Accustart II PCR Toughmix (Quantabio ...
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bioRxiv - Neuroscience 2020Quote: Binding kinetic of the purified Fabs to full length PrP was monitored at 25 °C using ProteOn XPR36 surface plasmon resonance biosensor (Bio-Rad Laboratories). Mouse recPrP23–231 was immobilized on a carboxymethylated-dextran sensor chip (CM5 ...
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bioRxiv - Molecular Biology 2023Quote: The interaction between nucleic acids (ligands) and the proteins (analytes) were measured by the surface plasmon resonance (SPR) technique using a ProteOn XPR36TM (Bio-Rad Laboratories). NLC Sensor chips precoated with NeutrAvidin (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... Optical densities (OD) were measured at 450 nm using an ELISA plate reader (Spectrophotometer Bio-Rad Xmark). Cut-off values were assessed as the average OD plus two standard deviations obtained from serum (n = 100 ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... RNA was obtained using the SingleShot Cell Lysis Kit (Bio-Rad) according to the manufacturer’s instructions and cDNA synthesis was performed using the iScript Advanced cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Systems Biology 2022Quote: RNA was isolated using the SingleShot Cell lysis kit (Bio-Rad). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... tubes were aspirated and Bio-Plex Cell-Lysis Kit (Bio-Rad) was added and spheroids were then sonicated ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR was performed on a CFX-96 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA, USA) using iTaq Universal SYBR® Green supermix (Bio-Rad, # 172-5121, USA) and primer pairs were synthesized by IDT (Coralville ...
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bioRxiv - Physiology 2022Quote: ... real-time quantitative polymerase chain reaction (RT-qPCR) was executed with 13 ng cDNA using SSO advanced SYBR Green supermix (Bio-Rad Laboratories, Hercules, CA, USA) with the following settings ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative RT-PCR was performed on a CFX-96 Bio-Rad reverse transcription polymerase chain reaction detection system (Hercules, CA, USA) using iTaq Universal SYBR® Green supermix (Bio-Rad, # 172-5121, USA) and primer pairs were synthesized by IDT (Coralville ...
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bioRxiv - Genetics 2021Quote: TA were isolated and cells were lysed using Bio-Plex Cell Lysis Kit (Bio-Rad, Hercules, CA). The amount of proteins of each sample was measured with Pierce BCA protein Assay Kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... washed 3x in TBS-T Tween and imaged on a Chemidoc Touch imaging system (BioRad) using Clarity ECL (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... blocked for 1 hour in 5% non-fat milk in TBS-T (Bio-Rad, USA), and incubated with primary antibodies anti-ACE2 (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... Development was performed after 3 washes in TBS-T and using Clarity ECL reagents (BioRad) and automatic exposure set up on ChemiDoc imaging system (BioRad).
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bioRxiv - Cancer Biology 2020Quote: ... 1/10 000 in TBS-T) and finally revealing thanks to ECL (Biorad 170-5161) under the ChemiDoc imaging system (BioRad).
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bioRxiv - Developmental Biology 2022Quote: ... before blocking in 5% milk (Marvel) in Tris Buffered saline Tween (TBS-T, Bio-Rad) for 60 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... All PCR reactions were performed using a Biorad T-100 Thermocycler (Bio-Rad, Hercules, CA). The V4-V5 region of the universal 16S rRNA gene was amplified in duplicate from 20 dates collected over the course of reactor operation using the 515F-Y/926R primer set 30 ...
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bioRxiv - Neuroscience 2020Quote: ... TBS-T) before incubation with HRP-conjugated secondary antibody for 2 hours (Biorad, 1:1000). After 3 times washes with 5 min intervals with TBS-T ...
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bioRxiv - Molecular Biology 2023Quote: ... then washed in TBS-T prior to incubation in Clarity Western ECL Substrate (Bio-Rad) and detection using the ChemiDoc (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... The amount of surface expressed receptors was detected by adding 60 µL wash buffer and 20 µL HRP substrate (Bio-Rad, 170-5060) per well ...
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bioRxiv - Biochemistry 2020Quote: Surface biotinylation samples were heated to 50°C for 10 minutes before separation using 4-20% polyacrylamide gradient gels (Bio-Rad, Hercules, CA). After separation ...