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Citations for Bio-Rad :
351 - 400 of 6495 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBS-T (3 × 5 minutes) and incubated in horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000) (BioRad) in blocking solution for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then washed three times with TBS-T for 5 min each before developing using Clarity MaxTM Western ECL Substrate (1705062; BioRad) and imaged using a BioRad ChemiDoc XRS+.
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bioRxiv - Molecular Biology 2023Quote: ... and incubated with the following secondary antibody diluted 1/5000 in milk PBS-T: Goat anti-rabbit IgG HRP conjugate (170-6515; BioRad) or Goat anti-mouse IgG HRP conjugate (170-6516 ...
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bioRxiv - Genetics 2022Quote: ... We washed the membrane 3× 5 minutes in TBS-T and 1× 5 minutes TBS before imaging (Bio-Rad ChemiDoc).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with secondary antibody goat anti-rabbit conjugated to HRP (1:5000, 5% wt/vol milk in TBS-T; #170-6515, BioRad) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies to the membrane for 1 h in the presence of 5% milk followed by four washes in TBS-T and developing with ECL substrate (1705061; BioRad). Blot signal was captured using the same BioRad ChemiDoc™ Touch Gel Imager ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The membrane was rinsed three times with TBS-T and then incubated with goat anti-mouse IgG coupled to horse radish peroxidase (1/10,000, 1706516, Bio-Rad) in TBS-T for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3x with 10 mL of TBS-T and developed with Clarity Max ECL substrate (Bio-Rad). Western blot images were taken with an Azure Biosystems C400 imager.
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bioRxiv - Developmental Biology 2024Quote: ... PVDF membranes were blocked for 1 hour in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4 °C with antibodies listed in Supplementary Table 2 in 5% BSA in TBS-T ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Absorption was read at 450 nm with wavelength correction set to 540 nm using an ELISA plate reader (Bio-Rad, Hercules, CA, USA). Intra- and inter-assay coefficients of variation were <10% for all EIAs.
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bioRxiv - Molecular Biology 2021Quote: ... The plates were incubated at 37°C for different time points (0,4hr,12hr,24hr,72hr) and was measured on an ELISA reader (Bio-Rad, Hercules, CA, USA) at a wavelength of 570 nm and O.D was recorded.
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bioRxiv - Cell Biology 2021Quote: ... or mRNA isolated from cell lines or primary mouse cells were proceeded to RT-qPCR using iTaq universal SYBR green one step kit (Bio-Rad, Cat. No. 1725150). DNA obtained from ChIP was proceed to RT-qPCR using iQ SYBR green supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were stained with an apoptosis and necrosis staining kit (ABD Serotech, Bio-Rad, Raleigh, NC) according to manufacturer’s instructions and analyzed by flow cytometry on a BD LSRFortessa ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from cells using the Aurum™ Total RNA Mini Kit (Bio-Rad). The spin protocol was employed according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... from whole zebrafish embryos or sorted cells and reverse transcribed with iScript cDNA Synthesis Kit (BioRad). For qPCR ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... cell extracts were quantified with the Bradford-based Protein Assay kit (Bio-Rad Protein Assay, #5000006). After addition of 6X Laemmli buffer (375 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... We dissociated the cells and extracted RNA using the Aurum Total RNA mini kit (Biorad, 7326820). We measured RNA concentration using a spectrophotometer and only included RNA that had an A260/280 >1.8 ...
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the cell extracts was determined using the BCA assay kit (Bio-Rad). Protein concentration for lysates was adjusted to 2 μg/μl before adding 4X loading buffer (ThermoFisher) ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from cells and tissue using Aurum™ Total RNA Mini kit (Bio-Rad) and reverse transcribed using iScript™ cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: Cytokines were measured in cell culture supernatants using luminex analysis (Biorad human 17-plex Bioplex kit) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the membranes were washed 3 times with 1 x TBS-T and antibody binding was detected in a BioRad Versa Doc System with the Quantity One software (BioRad, Germany) by chemiluminescence using the Clarity Western ECL Substrate (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed three times with PBS-T (15 min, RT) and chemiluminescence was detected after 10 min incubation with freshly prepared Clarity Western ECL Substrate (Bio-Rad) with a LAS-4000 gel scanning station (Fujitsu Life Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... USA) that were diluted 1/5000 in TBS-T and revealed by enhanced chemiluminescence plus (ECL) solution (Biorad Laboratories, ON, Canada). β-actin was used to ensure equal protein loading ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed again for 3 × 5-min in TBS-T before being immersed for 5 min under gentle agitation at room temperature in Clarity ECL detection substrate (Bio-rad Laboratories Pty Ltd ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were washed three times with T-TBS for 15 min and incubated with goat anti-rabbit HRP-conjugated secondary antibodies (Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBS-T and incubated with HRP-linked secondary antibodies prior to detection with Clarity ECL chemiluminescence reagent (Bio-Rad). All blots were imaged on a Bio-Rad ChemiDoc-Touch system ...
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bioRxiv - Molecular Biology 2020Quote: ... The blots were washed three times with TBS-T for 5 min each time before to development with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging on the ChemiDoc XRS+ System (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... membranes were washed in PBS-T for three times 5 min and developed through chemiluminescence using a ChemiDoc XRS (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed with TBS-T and incubated with HRP-linked secondary antibodies prior to detection with Clarity-ECL chemiluminescence reagent (Bio-Rad). All blots were imaged on a Bio-Rad ChemiDoc-Touch system ...
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed six times with PBS-T and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) (1:2000) (Bio-Rad) diluted in 2% horse serum/PBS for 1 hr at room temperature with agitation ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed for 5 min 3x with TBS-T and incubated with secondary goat anti-rabbit-HRP antibody (Bio-Rad) at a 1:10000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed a further 3 times in TBS-T and bound antibodies were visualised using the Clarity Western ECL substrate (BioRad; #1705061) with a ChemiDoc MP system (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... The blots were washed 3 times in PBS-T and the proteins were visualized using Clarity Western ECL blotting substrate (Bio-Rad) or SuperSignal West Femto (ThermoScientific) ...
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bioRxiv - Microbiology 2023Quote: Membrane was then washed three times with TBS-T and incubated with secondary antibody (Goat Anti-Rabbit IgG StarBright Blue 700 by Bio-Rad) diluted 1:3,000 in blocking solution for 45 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Blots were washed four times in TBS-T prior to detection with either ProSignal Dura (Prometheus) or Clarity ECL (Bio-Rad) substrate and exposure to radiography film or imaging on a C-Digit imager (LiCOR).
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bioRxiv - Microbiology 2023Quote: ... Blots were washed three more times in TBS-T before being developed with Clarity Max Western ECL reagent and imaged on a ChemiDoc XRS+ (Bio-Rad).
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bioRxiv - Physiology 2023Quote: ... The membranes were washed in TBS-T and developed using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo) in a ChemiDoc imager (Bio-Rad). We normalized the protein bands to beta-tubulin with Image Lab software (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody was washed away with PBS-T and stained proteins were detected by chemiluminescence (Clarity western ECL, Bio-rad). The western blot signal was acquired using Amersham Image Quant8000.
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bioRxiv - Plant Biology 2023Quote: ... the membrane was washed three times with T-TBS and twice with TBS for 5 min each and incubated with Clarity Western ECL substrate (Bio-Rad). After 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then washed with TBS-T and chemiluminescence was assessed with the BioRad ChemiDoc MP Imaging System (BioRad, 12003154).
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in PBS + 0.05% Tween-20 (PBS-T) and incubated in goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Bio-rad) diluted 1:5000 in blocking solution ...
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bioRxiv - Cancer Biology 2021Quote: ... dimethyl sulfoxide (DMSO) was added and the absorbance of each well was measured at 490 nm using an ELISA microplate reader (Bio-Rad, Hercules, CA, USA) (33).
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bioRxiv - Cancer Biology 2021Quote: RNA was isolated from melanoma cells using the Aurum Total RNA Mini Kit (Bio-Rad Cat# 7326820) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatants as whole-cell lysates (WCLs) were quantified using a DC Protein Assay kit (Bio-Rad). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were incubated with APC (Lynx Rapid APC antibody conjugation kit, Cat # LNK031APC, Bio-Rad Laboratories, Inc.)-conjugated cytokeratin 6 (CK6 ...
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bioRxiv - Genomics 2020Quote: ... Cells were embedded in thin low-melting-point agarose plugs (CHEF Genomic DNA Plug Kit, Bio-Rad). Subsequent handling of the DNA followed protocols from Bionano Genomics using the Bionano Prep Blood and Cell Culture DNA Isolation Kit ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed with TCA buffer and protein concentrations were determined using DC protein assay kit (BioRad). Protein separation was performed with a 7.5% SDS-PAGE and transferred to nitrocellulose at 100 V for 90 mins.
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bioRxiv - Physiology 2023Quote: ... each fiber was carefully submerged in 3 µL of lysis buffer (SingleShot Cell Lysis kit, Bio-rad), containing proteinase K and DNase enzymes to remove unwanted proteins and DNA ...
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bioRxiv - Microbiology 2024Quote: ... The protein concentration in the cell lysates was determined using a DCTM protein assay kit (BioRad, 5000111). The same amounts of proteins were run on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad ...
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bioRxiv - Molecular Biology 2019Quote: ... The blot was washed 4 times with TBS-T and subsequently incubated with a goat anti-mouse-HRP antibody (Bio-Rad #1706516) for 30 minutes ...