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Citations for Bio-Rad :
401 - 450 of 1234 citations for 6 Bromo benzo c isothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... The mixture was incubated at 37°C for 15 minutes (T100 Thermal Cycler, Bio-Rad). The enzyme and the expressed proteins were inactivated by adding 15mM EDTA (Catalog No ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μl was pre-warmed to 50 °C and embedded into an agarose plug (BioRad). The plugs were incubated overnight in 42 °C in proteinase K lysis buffer (2 mg/ml proteinase K ...
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bioRxiv - Cell Biology 2023Quote: ... and denatured at 95°C for 5min with 4x Laemmli Sample Buffer (Cat. 1610747, BioRad). Samples were loaded onto Mini-PROTEAN® TGXTM Gels (Cat ...
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bioRxiv - Biochemistry 2023Quote: ... and placed at 4°C during acquisition on either a Ze5 (Everest software; Bio-Rad) or a MACSQuant VYB (MACSQuantify software ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C followed by horseradish peroxidase-conjugated secondary IgG antibody (Bio-Rad Laboratories) for 1h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured at 95 °C for 5 min in Laemmli sample buffer (Bio-Rad) containing 2.5% v/v 2-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... Complementary (c) DNA was then synthesized by using a qScript cDNA synthesis kit (Bio-Rad). Expression of SARS-CoV-2 S gene and mouse inflammatory cytokine and chemokine genes (IL-1β ...
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bioRxiv - Microbiology 2024Quote: ... at 4 °C for 30 min in a 10 ml poly-prep column (Bio-Rad). The column was then stabilized for 10 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 1 min at 95 °C) using iScript Reverse Transcription Supermix for RT-qRCR (Biorad, 1708840). The resulting cDNA was diluted by adding 24X volume of DPEC water subjected to qPCR using SsoAdvancedTM Universal SYBR Green Supermix (Biorad ...
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bioRxiv - Cancer Biology 2024Quote: ... These samples were denatured at 100 °C in 4 × XT Sample Buffer (Bio-Rad, USA). N-glycosylation modification of proteins was assessed using the PNGase F Glycan Cleavage Kit (A39245 ...
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bioRxiv - Microbiology 2023Quote: ... The reactions were incubated at 65 °C for 5 min in a thermocycler (Bio-Rad) and chilled on ice for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... IB analysis was performed after 6-7.5% SDS-PAGE or 4-20% Mini-PROTEAN TGX Precast Protein Gels (BioRad, Hercules, CA), with overnight incubation with a 1:1000 dilution of primary antibody and followed by a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibody (Jackson ImmunoResearch ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... antigen retrieval was performed in trypsin (pH 7.8) or 10 mM sodium citrate buffer (pH 6) and then incubated with F4/80 antibody (1:50; MCA497, Bio-Rad) or UCP-1 antibody (1:500 ...
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bioRxiv - Microbiology 2019Quote: ... The libraries were quantified and checked for amplifiable adapters using the Library Quantification DNA standards 1-6 (Kappa Biosystems, Wilmington, USA) with the SsoFast EvaGreen qPCR supermix (Bio-Rad) using 10 μL EvaGreen master mix ...
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bioRxiv - Bioengineering 2019Quote: Equal volumes of (GT)6-Cy5-SWCNT and FAM-tagged protein at 2X working concentration were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Bioengineering 2020Quote: ... Equal volumes of 10 mg/L (GT)15- or (GT)6-Cy5-SWCNTs and 160 mg/L protein were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Biophysics 2021Quote: ... beads were washed with wash buffer 6-8 times in poly-prep chromatography columns (cat. no. 7311550, BIO-RAD laboratories Inc). Protein was eluted using Ni-NTA elution buffer (50□mM NaPi pH 7.6 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (20 µg/lane) were electrophoresed on a 6–12% SDS polyacrylamide gel in a Mini-PROTEAN Tetra Cell (Bio-Rad) and subsequently transferred to a nitrocellulose membrane (Sartorius AG ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described 58 and a custom Bio-Rad 6-plex based on the Human Inflammation Panel 1 (BioRad, Cat# 171AL001M). For both assays ...
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bioRxiv - Cell Biology 2021Quote: ... inside an anaerobic glove-box and the excess DTT was completely removed via a Bio-Gel P-6 DG gel-desalting column (Bio-Rad) in thoroughly de-oxygenated MOPS buffer (20 mM ...
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bioRxiv - Biophysics 2021Quote: ... DIV15-30 neurons were transfected 2-6 days before imaging via biolistic gene transfer (160 psi, Helios gene gun, Bio-Rad), as described in a detailed video protocol (Woods and Zito 2008) ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by vertical gel electrophoresis on a 6% (60:1 Acrylamide/Bisacrylamide) native gel column using a 491 Prep Cell (Bio-rad) run at 10W and 4°C ...
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bioRxiv - Microbiology 2019Quote: ... 4 µl of the diluted DNA sample was added to 6 µl of a mixture containing SYBR Green Supermix (Bio-Rad) and gene-specific primers (0·4 µM total ...
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bioRxiv - Biophysics 2019Quote: ... 30 μl of purified protein in 0.02% DDM at ∼1 mg/ml was buffer exchanged into 200 mM ammonium acetate pH 7.5 and 0.02% DDM using Biospin 6 gel filtration spin columns (Bio-Rad, Hercules, CA). 2 μl of buffer exchanged ELIC was loaded into a borosilicate capillary emitter (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: A549 or DF-1 cells in 6-well plates were infected with the indicated IAVs and cells were lysed with 2X Laemmli sample buffer (Bio-rad) plus 2% beta-mercaptoethanol at 8 hpi ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and 50 µl of a 10−4 dilution was loaded into selective ura− agar in a 6 cm petri plate and subjected to UVC radiation in a UVP gel Imaging and Documentation device (Bio-Rad) for 15 and 30 sec ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with SINV at an MOI of 2 and pictures were taken 6 hours post-infection using ZOE fluorescent cell imager (Bio-Rad). Proteins were collected with lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% (w/v) SDS and 6% (v/v) β-mercaptoethanol) and protein concentrations were quantified using Bradford Protein Assay (Bio-Rad). Equal concentrations of protein were fractionated on NuPAGE 4-12% Bis-Tris Protein Gels ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were dried out at RT and then exposed to a phosphoscreen cassette (GE) for 6 days for further scanning in a phosphoimager analyzer (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... chromosomal DNA plugs were prepared and separated on 1% agarose gel at 6 V/cm for 48 hrs (initial time = 20s, final time = 30s) by using the CHEF DRII apparatus (Bio-Rad), followed by Southern blotting and hybridization with a TRP1 sequence as a probe.
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bioRxiv - Genomics 2022Quote: ... and 20 ug of each sample were loaded on a 6% or 10% SDS-PAGE gel and transferred to a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz ...
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bioRxiv - Biochemistry 2023Quote: ... The 6× His-tag blots were washed with TMST and TSM and developed with the Clarity Western ECL kit (Bio-Rad) and imaged in a Gel-Doc system (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... 10 µg of RNA was transfected into 4×10^6 S10-3 cells by electroporation with a Gene Pulser II apparatus (Bio-Rad) in 0.4 cm Gene Pulser cuvettes (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was separated by non-reducing SDS-PAGE (6% acrylamide gel) with Precision Plus Protein Dual Color Standards (Bio-Rad) and transferred to nitrocellulose membrane overnight at 4 °C ...
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bioRxiv - Physiology 2023Quote: ... The incorporated radioactive methionine and cysteine signal was detected using Typhoon Imager (GE) and quantified using Image Lab 6 software (Bio-Rad). Afterwards ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs isolated from two donors were infected with GFP-EBV for 28 days in 6-well plates and subsequent B-cells blasts were photographed using using a ZOE Fluorescent Cell Imager (BIO-RAD). Using similar strategy ...
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bioRxiv - Cell Biology 2023Quote: Samples were diluted in 5x loading buffer and ∼30 mg run out on a 6-14% SDS-PAGE gel as described before semi-dry Western blotting (BioRad TurboID). Ponceau stained images were taken ...
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bioRxiv - Molecular Biology 2024Quote: ... or immunoprecipitated samples were resolved by SDS-PAGE (6% to 10%) and transferred onto methanol-treated immun-blot PVDF membrane (Bio-Rad). The membranes were incubated overnight at 4°C with corresponding primary antibodies (antibody information ...
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bioRxiv - Immunology 2024Quote: ... The gel was imaged under the Silver Stain setting on Image Lab 6 on a Gel Doc EZ Imager (Bio-Rad). ImageJ was used to quantify band intensities and determine peptide loading efficiencies.
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bioRxiv - Cell Biology 2022Quote: ... Plates were imaged after 3 days at 30°C using the Chemidoc Touch imaging system (BioRad) on colorimetric setting.
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bioRxiv - Cell Biology 2022Quote: ... at 4 °C for overnight followed by incubation with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad) and detection using Amersham™ ECL™ Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2020Quote: ... at 95°C for 10 min and separated by a Mini-PROTEAN® TGX (Bio-Rad) precasted gel (4-20% acrylamide) ...
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bioRxiv - Cell Biology 2021Quote: ... boiled at 95°C for 5min and loaded in 4-15% acrylamide gels (Bio-Rad, 5678084). After electrophoresis ...
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bioRxiv - Biochemistry 2020Quote: Chromatography was conducted at room temperature (∼22°C) using NGC medium-pressure chromatography systems from BioRad Laboratories Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... denatured at 95°C for 5 min and loaded onto Mini-PROTEAN TGX Gels (Bio-Rad). Samples were transferred to nitrocellulose membranes using the TransBlot Tubro system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled at 65 °C for 10 minutes and separated by SDS-Page (Bio-Rad 4–20 % Mini-PROTEAN® TGX Stain-Free™ protein gel) ...
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bioRxiv - Cell Biology 2020Quote: ... then heated at 90°C for 5 min before loading onto 4-20% gel (Bio-Rad). Proteins were separated using running buffer (Bio-Rad ...
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bioRxiv - Bioengineering 2020Quote: ... The real time PCR assays were run on Bio-Rad C-1000 Touch (Bio-Rad, USA) Real-Time PCR System ...