Labshake search
Citations for Bio-Rad :
351 - 400 of 1234 citations for 6 Bromo benzo c isothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: Samples were denatured at 95°C for 10 min in Laemmli buffer (Bio-Rad) with 2% β-mercaptoethanol and the indicated amount of proteins were resolved by SDS–PAGE and transferred onto PVDF membrane (Immobilon-P ...
-
bioRxiv - Immunology 2024Quote: ... Reaction was performed for 2h at 37°C in nuclease-free microcentrifuge tubes (BioRad). A260/295 was measured with a nanodrop (company ...
-
bioRxiv - Biophysics 2021Quote: ... The uncoupled BG-GLA-NHS was removed by filtration on Micro Bio-Spin™ P-6 Gel Columns (Bio-Rad).
-
bioRxiv - Molecular Biology 2020Quote: ... approximately 250.000 cells were plated in a 6-well dish and transfected with a final concentration of 22.5 nM siRNA using siLentFect Lipid Reagent (Bio-Rad) accordingly to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... MtCoaBC and MsCoaBC were exchanged into NH4OAc (500 mM, pH 7.0) solution using Micro Bio-Spin 6 chromatography columns (Bio-Rad). A sample volume of 2.5 μL was injected into a borosilicate emitter (Thermo Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... YpdA and YpdAC14A proteins were pre-reduced with 10 mM DTT followed by DTT removal with Micro Biospin 6 columns (Biorad). For the biochemical activity assays of the specific BSSB reductase activity ...
-
bioRxiv - Biophysics 2019Quote: ... We then removed excess dye by four subsequent spins of the solution over Micro Bio-Spin 6 Columns (Bio-Rad) at 700 g for 2 min ...
-
bioRxiv - Molecular Biology 2019Quote: ... EtOH precipitation was performed and DNA was additionally cleaned up on Micro Bio-Spin 6 chromatography columns in SSC (BioRad) as purity was essential for oxidation of hmC ...
-
bioRxiv - Molecular Biology 2020Quote: DNA isolated from cells by phenol:chloroform isolation and ethanol precipitation was cleaned on Micro Bio-Spin P-6 SSC columns (Bio-Rad) according to manufacturer protocol ...
-
bioRxiv - Biochemistry 2019Quote: ... The spin-labeled protein was further concentrated and free label was removed using Micro BioSpin 6 Chromatography Columns (Bio-rad) pre-equilibrated with buffer C.
-
bioRxiv - Plant Biology 2020Quote: ... Captured elution fraction from the Strep-Tactin-affinity purification were loaded onto a anion exchange column (UNO Q-6, Biorad) with a flowrate of 4 ml/min ...
-
bioRxiv - Biochemistry 2020Quote: ... and CyaY were buffer exchanged into 200 mM ammonium acetate (pH = 7.5) using Micro Bio-Spin 6 Columns (Bio-Rad). Instrument parameters were tuned to maximize transmission but simultaneously preserve the native-like state of proteins as determined by ion mobility ...
-
bioRxiv - Neuroscience 2020Quote: ... IL-6) were quantified using a real-time PCR detection system and SsoAdvanced Universal SYBR Green Supermix kit (BIO-RAD). In brief ...
-
bioRxiv - Biophysics 2020Quote: ... Fractions containing protein were pooled and desalted to remove imidazole by gel filtration on a 50 mL BioScale P-6 column (BioRad) using resuspension buffer as the eluant.
-
bioRxiv - Biochemistry 2020Quote: ... the cleaved His6 tag was removed and the enzymes were buffer exchanged into 200 mM ammonium acetate (pH 7.5) using Micro Bio-Spin 6 columns (Bio-Rad). His6-affinity tagged HalA2 was likewise digested with thrombin to remove the His6 tag ...
-
bioRxiv - Plant Biology 2021Quote: ... The IEF was performed on the 7cm length gel strips with immobilized pH gradients 3-10 and 3-6 (BioRad). After IEF ...
-
bioRxiv - Cancer Biology 2022Quote: Proteins were resolved in 6-8-10 or 12% polyacrilammide gels and transferred to PVDF (Bio-Rad, Hercules, CA,USA) or nitrocellulose membranes (GE Heath Care ...
-
bioRxiv - Biochemistry 2022Quote: ... the enzymes were buffer exchanged into their respective reaction buffers above using a Micro Bio-Spin 6 size exclusion spin column (Biorad) to remove glycerol and other ion suppressing constituents ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to PVDF membrane at 4°C for 14.5 h at 30 V using the mini trans-blot module in transblot buffer (10 mM MES buffer pH 6 and 10% (v/v) methanol) according to the supplier’s instructions (Bio-Rad). The membrane was removed from the cassette ...
-
bioRxiv - Biophysics 2021Quote: ... and 2 times the critical micelle concentration (CMC) of various detergents (namely LDAO and C8E4) using a Biospin-6 column (BioRad) and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
-
bioRxiv - Developmental Biology 2019Quote: ... Alcian Blue was extracted by 6 M guanidine hydrochloride solution and measured by optical density at 600 nm (O.D. 600) using spectrophotometer (Bio-Rad).
-
bioRxiv - Cell Biology 2020Quote: ... gossypii proteins) or TBS (S. cerevisiae proteins) supplemented with 1 mM DTT using pre-equilibrated BioSpin-6 spin columns (BioRad) or ...
-
bioRxiv - Systems Biology 2021Quote: ... and 6 μl for slow growing mutants with lower OD600) on Stain-Free gels (4-20%, CAT # 4568096, Bio-Rad, Tris/Glycine SDS Buffer (CAT # 161-0732 ...
-
bioRxiv - Neuroscience 2019Quote: ... Serum was isolated and IL-6 levels were measured using a Bio-Rad Luminex system (Bio-Rad, Laboratories, Hercules, CA) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2021Quote: APRT2-Ntag and APRT2-Ctag were buffer exchanged into a volatile buffer (200 mM ammonium acetate, pH 7.4) using a Micro Bio-Spin 6 device (Bio-Rad). Samples were loaded into pulled glass capillaries prepared in house ...
-
bioRxiv - Immunology 2021Quote: ... before buffer exchange into 500 mM ammonium acetate solution (pH 7.5) using a Micro Bio-Spin 6 column (Bio-Rad).
-
bioRxiv - Biochemistry 2022Quote: ... were exchanged from size exclusion buffer into 2 M ammonium acetate pH 7.4 via a single-pass through a Bio-Spin P-6 column (Bio-Rad). Buffer-exchanged protein was loaded into gold-coated glass capillaries (1.0 mm OD/0.75 mm inner diameter with filament) ...
-
bioRxiv - Biochemistry 2023Quote: ... CsgA and CsgAslowgo samples were buffer-exchanged into MS-compatible buffer (150 mM Ethylene diamine diacetate pH 6.3, 10mM TMAO) using Micro Biospin 6 desalting columns (Bio-Rad) to a final protein concentration of 3-5 μM ...
-
bioRxiv - Plant Biology 2023Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE (pH 8.0) buffer and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
-
bioRxiv - Biochemistry 2023Quote: ... The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad). Molecular weight of standard was as follows ...
-
bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
-
bioRxiv - Molecular Biology 2024Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE buffer (pH 8.0) and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
-
bioRxiv - Cell Biology 2020Quote: ... 60 °C for 1 min for 40 cycles and analyzed using 7900HT SDS software (Biorad). Each run consisted of triplicate technical repeats per experiment ...
-
bioRxiv - Microbiology 2020Quote: ... and gradually cooled to 25°C over 45 minutes in a S1000 Thermocycler (Bio-Rad) and stored at −20°C ...
-
bioRxiv - Cell Biology 2021Quote: ... in a 20 μl reaction on a thermal cycler (C-1000 Touch; Bio-Rad Laboratories). Product sizes were controlled by DNA gel electrophoresis and the melt curves were evaluated using CFX Manager (Bio-Rad Laboratories) ...
-
bioRxiv - Microbiology 2022Quote: The concentrations of the 37°C samples were determined with a DC Protein Assay (BioRad). A volume corresponding to 50 μg of the 37°C sample was used for further analysis ...
-
bioRxiv - Neuroscience 2021Quote: ... heated at 70°C for 10min and loaded into 4-20% precast gels (Bio-rad) for electrophoresis (130V ...
-
bioRxiv - Biochemistry 2020Quote: ... SEC purification was performed at 4°C on an Enrich SEC 650 column (Bio-Rad) and sample eluted in SEC buffer (20 mM HEPES ...
-
bioRxiv - Cancer Biology 2022Quote: ... Lysate was clarified by centrifugation at 4 °C and protein quantified using bradford (Bio-Rad) assay ...
-
bioRxiv - Microbiology 2019Quote: ... 120° included angle and 14°C constant temperature in a CHEF-MAPPER system (Bio-Rad) with pulsed times ramped from 0.2s to 120s.
-
bioRxiv - Biochemistry 2021Quote: ... Gels were dried (3 hr, 80 °C) on a Model 583 Gel Dryer (Bio-Rad) and exposed to a phosphor screen ...
-
bioRxiv - Genetics 2022Quote: ... for 5 mins at 95°C and loaded onto TGX stain-free precast gels (Biorad). Western blots were carried out using the following antibodies ...
-
bioRxiv - Neuroscience 2022Quote: ... The samples were incubated 5 min at 65°C in C1000 Thermal Cycler (Bio-Rad). Then ...
-
bioRxiv - Neuroscience 2022Quote: ... heating the aliquots at 95°C for 90 seconds in a thermal cycler (Bio-Rad), and cooling them for at least 30 minutes at room temperature in the dark (i.e ...
-
bioRxiv - Cancer Biology 2022Quote: ... annealing at 55°C for 30s using CFX Connect Real-Time PCR system (Bio-Rad). The endogenous actin gene (1301 nt of Ov-actin mRNA ...
-
bioRxiv - Microbiology 2022Quote: ... 120° included angle and 14 °C constant temperature in a CHEF-MAPPER system (Bio-Rad) with pulsed times ranging from 0.5 s to 3 min 10 s with a linear ramping factor.
-
bioRxiv - Plant Biology 2023Quote: ... and hold at 4°C in a MyCycler thermal cycler (Bio-Rad, Hercules, CA, USA). PCR products of the three reactions were pooled into a single sample ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were denatured at 95°C for 10 minutes in Laemmli buffer (Bio-Rad, #1610747) with 2% β-mercaptoethanol (Sigma ...
-
bioRxiv - Plant Biology 2023Quote: ... for 1 h at 4 °C and incubated with protein-G magnetic beads (Bio-rad) for 0.5 h at 4 °C ...
-
bioRxiv - Microbiology 2023Quote: ... The gel was dried for 2h at 60°C using Gel Dryer 583 (Bio-Rad) and visualized on developing film overnight at room temperature.