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Citations for Bio-Rad :
4501 - 4550 of 7321 citations for 4S 6 8 difluoro 3 4 dihydro 2H 1 benzopyran 4 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The protein extracts were resolved by 12% (w/v) SDS-PAGE using a Bio-Rad Mini PROTEAN 3™ electrophoresis unit (Bio-Rad) and electrotransferred onto Hybond ECL nitrocellulose sheets (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The primer sequences for detecting ACTB are 5’-GACCTGACTGACTACCTCAT-3’(forward) and 5’-TCTCCTTAATGTCACGCACG-3’ (reverse) Quantitative PCR (qPCR) reactions were conducted using the SYBR green PCR master mix (Bio-Rad, 1725121) in CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Gel was transferred at 350 Amperes for 120 minutes onto pre-activated polyvinylidene difluoride (PVDF) or Nitrocellulose membranes in a Mini-Protean® 3 Cell (BioRad Laboratories). Correct protein transfer was checked by red Ponceau S Solution (Panreac) ...
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bioRxiv - Cell Biology 2024Quote: ... and then transferred onto a polyvinylidene fluoride (PVDF) membrane at constant voltage of 25V for 3 min using Trans-Blot TurboTransfer Packs (Cat.: 1704156, Lot.: 64340580, Bio-Rad, USA) and Trans-Blot Turbo system (Cat. ...
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bioRxiv - Physiology 2021Quote: ... and mixed 1:1 with 2x Laemmli buffer (Bio-Rad) plus 5% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: ... The blot was stained with 1:1 enhancer:substrate reagents (BioRad) for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample diluted 1:1 with Laemmli Sample Buffer (BioRad) mixed with 350 mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... Protein was denatured with 1:1 Laemlli loading buffer (BIORAD) by boiling for 10 minutes on a dry bath (Thermolyne Dri-Bath ...
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bioRxiv - Biochemistry 2023Quote: ... The collected cells were mixed 1:1 with TrypanBlue (BioRad) and counted.
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bioRxiv - Genetics 2023Quote: ... 1:500 to 1:1000 anti-rabbit (BioRad, 172-1019). Signal was detected using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Systems Biology 2021Quote: Differential expression of ten selected transcripts in response to virus at 3-L1 stage was examined using Droplet Digital PCR (ddPCR) (Bio-Rad, Hercules, CA). Because of the difficulty in obtaining a large amount of gut RNA samples and the limited sensitivity of conventional qPCR technique to detect gene expression at a low level ...
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bioRxiv - Neuroscience 2022Quote: ... The embryoid media was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Anti-Anti, 55 mM 2-mercaptoethanol (BioRad; Cat. No. 1610710), 100 ng/mL M-CSF ...
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bioRxiv - Cell Biology 2021Quote: ... Band density was normalized according to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) content and analyzed using Image Lab software (Bio-Rad, California, USA) (29).
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bioRxiv - Plant Biology 2021Quote: ... Membranes were washed 3 x 10min in TBS + Tween 20 before proteins were detected by Immuno-Star luminol-peroxide (Bio-Rad, #170-5070). Bio-Rad Chemi-doc was used to visualize and quantify proteins with densitometry.
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 3% H2O2 for 15 min at room temperature and then incubated with goat anti-rabbit IgG-HRP (Bio-Rad 170-6515) secondary antibody diluted 1:300 in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Genomics 2024Quote: ... 4.5 µl of sample were used to generate single cell RNA-Seq libraries by following the Illumina Bio-Rad SureCell WTA 3’ Library Prep Guide (Illumina, San Diego CA and Bio-Rad, Hercules, CA) and using the ddSEQ Isolator for Single Cell Sequencing (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell suspension then was divided equally and heated for 3 min at indicated temperatures on T100™ Thermal Cycler (Bio-Rad Laboratories). Cells were snap-frozen and thawed twice and centrifuged at 17.000 g for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were denatured at 95 °C for 3 minutes prior to loading onto a 16.5% Mini-PROTEAN® Tris-Tricine Gel (Biorad Cat. No. 4563066). Gels were transferred to a nitrocellulose membrane (Millipore Sigma Cat ...
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bioRxiv - Biophysics 2023Quote: ... Migration of the DNA in the gel was visualized via the fluorescent Cy-3 label (ChemiDoc™ MP Imaging System, BIO-RAD, USA). Protein-DNA complexes were transferred from the gel to the PVDF membrane (Immun-Blot® PVDF Membrane ...
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bioRxiv - Molecular Biology 2024Quote: ... 3-10 μg total protein was loaded and run on AnykD™ Criterion™ TGX™ Precast acrylamide protein gels (Biorad cat#5671125) then transferred to nitrocellulose (Biorad cat#1704159) ...
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bioRxiv - Microbiology 2024Quote: ... and R-tot (5′-CTGCTGCCTCCCGTAGGAGT-3′) in the Rotor Gene 6000 thermocycler (Corbett Research, Sydney, Australia) with the iTaq Universal SYBR Green Supermix (Bio-Rad, Reinach, Switzerland).
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bioRxiv - Biophysics 2024Quote: ... His-tagged protein was bound to 3 mL HisPur™ Ni-NTA Resin (Thermo 88221) equilibrated with lysis buffer in a glass Econo-Column® (Bio-Rad) by flowing through the clarified lysate ...
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bioRxiv - Immunology 2021Quote: ... (BioRad Cl:A3-1) and goat anti-rat IgG cross-absorbed Alexa Fluor® 488 secondary antibody (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 (BioRad Laboratories) and normalized the loading control.
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts (1 mg) of protein were diluted 1:1 in sample loading buffer (2x, Bio-Rad, UK) for each extraction method and loaded onto a 12% Tris-glycine pre-cast SDS gel (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... secondary antibodies (1:10000; 1:50000 for anti-H3 antibody, BioRad) in 5% milk/PBST at RT ...
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bioRxiv - Cell Biology 2020Quote: ... and 1:500 to 1:1000 anti-rabbit (BioRad, 172–1019). Signal was detected using Pierce ECL Western Blotting Substrate (Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... and F4/80 (dilution 1:200, clone A3-1, Bio-Rad); ER2 EDTA for CD3 (dilution 1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:10000; 1:50000 for anti-H3 antibody, BioRad) in 0.1% Tween-20 in 1x PBS (PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:10000; 1:50000 for anti-Gapdh antibody, BioRad) in 5% milk/PBST at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were mixed 1:1 with 2x Laemmli sample buffer (BioRad) supplemented with 5% □-ME (Sigma ...
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bioRxiv - Genetics 2023Quote: ... and mixed 1:1 with 2X Sample Loading Buffer (Bio-Rad) + 5% β-mercaptoethanol ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were diluted 1:1 with 2× Laemmli buffer (Bio-Rad) supplemented with 54 mg ml-1 DTT ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked for 1 hour in 1% casein (Bio-Rad) in PBST (0.1% Tween-20 in PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... at 1:2000 in filtered PBS with 1% casein (Biorad 1610783). Goat-anti-mouse IgM secondary antibody (CalBiochem cat# 401225 ...
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bioRxiv - Biochemistry 2020Quote: To visualize protein expression 1 μL of the TXTL reactions was mixed with 2 μL of water and 3 μL of 2x Laemmli loading dye (Bio-Rad, Hercules, CA, USA) and incubated at 90°C for 3 min ...
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bioRxiv - Systems Biology 2021Quote: ... incubated 5 min at 65 °C and separated by 12% SDS-PAGE (65) in the Mini Protean 3 Apparatus (BioRad Laboratories, Hercules, CA, USA). The final concentration of proteins was 10 μg per lane ...
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bioRxiv - Genetics 2022Quote: ... the membranes were washed 3 times for 10 min each with TBST buffer at 24 °C and visualized using enhanced chemiluminescence reagents (Bio-Rad Laboratories, CA, USA). The images were captured using Image Quant LAS 4000 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... and O’nyong yong virus (ONNV) were subjected to a thermal gradient treatment from 30 to 60 °C for 3 h with a thermocycler (Bio-Rad T100 Thermal Cycler), after which samples were immediately titrated on Vero cells ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, 1725121). Each reaction proceeded at 50 °C for 2 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and the miR200c primers (upstream primer, 5’- TAATACTGCCGGGTAATGATGGA-3’) (Eurofins Genomics) on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). U6 primers (TAKARA ...
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bioRxiv - Molecular Biology 2024Quote: ... The same AAV2 ITR primers were used for ddPCR along with a FAM labeled probe 5 ’-CACTCCCTCTCTGCGCGCTCG-3 with the QX200 Bio-rad Laboratories’ droplet digital system (Bio-rad Laboratories, California, USA) and ddPCR supermix for probe (Bio-rad Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were incubated with the primary antibodies (rabbit anti-cPLA2, 1:100, Cell Signalling; mouse anti-LaminA/C, 1:500, ABCAM; rat anti-tyrosinated α-tubulin 1:500, Bio-Rad; mouse γ-H2AX, 1:2000) in blocking solution for 1h at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with horseradish peroxidase conjugated secondary antibodies (1:5,000 or 1:10,000 in non-fat dry milk, 1 h, room temperature; Bio-Rad Laboratories). Secondary antibodies were visualized using enhanced chemiluminescence (ECL Plus ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore; Goat anti-rabbit HRP 1:3000, BioRad)
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bioRxiv - Immunology 2023Quote: ... Antigen-coupled microspheres (1000/well) were incubated with serially diluted samples (1:100, 1:1000, 1:10,000) in replicates in Bioplex plates (Bio-Rad) at 4°C for 16hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 1:3000 or 1:10000 (for H3) dilutions of secondary antibodies (BioRad) (1 h at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... according to 1:1 by adding appropriate amount of ECL (Bio-Rad) color solution in the dark conditions and photographed using an EZ-ECL chemiluminometer Detection Kit for HRP (Biological Industries ...