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Citations for Bio-Rad :
4251 - 4300 of 4995 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Equal amounts of protein from (30 μg, mouse cortex was used) were separated by electrophoresis in precast 4-12% Bis-Tris Gels (Bio-Rad) and transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2020Quote: ... 1μg of pKSB vector containing aimed mutation and the bacterial liquid were mixed at 4℃ and electroporated by Micropulser (Bio-Rad) with Ec2 (2.5kV ...
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bioRxiv - Neuroscience 2020Quote: ... After rinses with PBS-0.1% Tween 20 (PBST), sections were incubated overnight at 4°C with rabbit anti-Iba1 (Wako, 019-19741) and anti-BrdU (Biorad, MCA2060). After washes with PBST ...
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bioRxiv - Neuroscience 2021Quote: ... samples were separated by electrophoresis on 4-20% Mini-PROTEAN® TGX Stain-Free™ gels (Bio-Rad Laboratories, NSW, Australia) in running buffer (0.1% (w/v ...
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bioRxiv - Cell Biology 2021Quote: Enriched CCV fractions were resolved via 1D SDS-PAGE on a 4-15% Tris-HCl gradient gel (BioRad cat# 161-1158) at a constant 200V for ∼90 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Thirty micrograms of each sample were mix with sample buffer loaded and separated using Mini-PROTEAN TGX 4-15% polyacrylamide gels (BioRad: 4561083) and Tris/Glycine/SDS buffer (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were subsequently blocked with 4% BSA and 1% Triton X-100 in PBS and incubated with rat anti-CD68 (1:500; Bio-Rad) and mouse-anti-GFP conjugated to Alexa Fluor 488 (1:250 ...
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bioRxiv - Bioengineering 2021Quote: ... Protein purity was evaluated by SDS-PAGE using 4–20% Mini- PROTEAN® TGX Stain-Free™ Gels (BioRad; Cat#17000435).
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bioRxiv - Biochemistry 2021Quote: ... The cell extract was clarified by filtering through 0.4 µm and 0.22 µm filters prior to loading onto ~4 mL of Ni-NTA resin (Profinity™ IMAC resin, Bio-Rad). To allow the His-tag protein to bind the resin it was incubated overnight at 4°C on a rolling shaker ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:4 with nuclease-free water and used as template (4 µL per reaction) for segment-specific qPCR using SsoFast EvaGreen Supermix (Bio-Rad) in 10 µl reactions ...
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bioRxiv - Neuroscience 2021Quote: Equal volumes of ISF were boiled at 98°C for 5 min in 50 mM of DTT and 1X LDS and separated by electrophoresis on 4-12% Bis-Tris Precast Gels (BioRad #3450124). Proteins were transferred onto 0.2 μm PVDF membranes (Biorad #1704157 ...
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bioRxiv - Neuroscience 2022Quote: ... 10µg of protein per sample was loaded into 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and run at 105V for 90 minutes ...
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bioRxiv - Physiology 2022Quote: ... we ran similar amounts of protein per lane (~10-20 μg, depending on target) on a 4-20% Criterion TGX stain-free gel (Bio-Rad) at 200 V for 50 min with the cassette surrounded by ice ...
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bioRxiv - Immunology 2022Quote: ... boiled for 10 min and subjected to protein separation by SDS-PAGE in 4 – 20% Mini-PROTEAN TGX precast gels (Bio-Rad) before transferred to nitrocellulose membranes (Wako) ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 10% neutral buffered formalin at 4°C overnight and then mCherry-positive plaques were visualized using a ChemiDoc MP imaging system (Bio-Rad). Afterwards ...
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bioRxiv - Biochemistry 2022Quote: ... Both input and IP-ed samples were resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad) and analysed by Western blot using specific antibodies.
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bioRxiv - Biochemistry 2022Quote: ... Input and MOV10/IgG IP-ed samples were resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad) and analysed through Western blot using specific antibodies.
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bioRxiv - Biochemistry 2022Quote: ... heated at 95 °C for 5 min and resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad). Proteins on the gel were transferred to the PVDF membrane ...
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bioRxiv - Developmental Biology 2022Quote: ... The suspended eggs and siRNA (5 μg/μl stock solution, in MilliQ) were transferred to a 4 mm cuvette (Bio-Rad) with a final volume of 200 μl and were gently mixed ...
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bioRxiv - Synthetic Biology 2020Quote: ... The boiled samples were centrifuged at 14,000 rpm for 10 minutes and 20 μg of total protein per sample was then loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA) and ran at 140 V ...
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bioRxiv - Pathology 2020Quote: ... The correct size of the purified RBD-scFv protein (52.4 kDa → ∼57 kDa with glycosylation) was confirmed by protein gel electrophoresis using Mini-PROTEAN TGX Precast Gels 4-20% (Bio-RAD).
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bioRxiv - Synthetic Biology 2020Quote: ... 10 to 25 μg for experiments that were imaged digitally) was loaded onto a 4–15% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run either at 50 V for 10 min followed by 100 V for at least 1 h ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with DTT (0.1 M endconcentration) and then subjected to SDS-PAGE on 12% or 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to PVDF membranes (TransBlot® TurboTM LF PVDF ...
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bioRxiv - Pathology 2020Quote: ... 5 microliters of urine were run on 4-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and stained with Coomassie Blue Stain Solution (Bio-Rad) to detect proteinuria ...
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bioRxiv - Biochemistry 2020Quote: ... in 1X TBE buffer at 220V for ∼13 min at 4°C and were imaged with a Gel Doc XR+ (Bio-RAD) and Quantity One 4.6.9 software (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Binding reactions were incubated for 40 minutes at 37 °C then run on a precast 5% TBE acrylamide gel at 4 °C for 45 minutes at 100 V (Bio-Rad). After transfer to Biodyne B Modified Nylon Membrane (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... The second-dimension gel electrophoresis was performed in 1× TBE buffer containing 0.3 μg/ml of ethidium bromide at 6.0 V/cm for 5 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). DNA was transferred to Hybond-XL (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... The lysates were loaded onto 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Cat# 4561086, Bio-Rad, Hercules, CA), and proteins were subsequently transferred onto a polyvinylidene fluoride (PVDF ...
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bioRxiv - Systems Biology 2021Quote: ... and 6 μl for slow growing mutants with lower OD600) on Stain-Free gels (4-20%, CAT # 4568096, Bio-Rad, Tris/Glycine SDS Buffer (CAT # 161-0732, BioRad)) ...
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bioRxiv - Molecular Biology 2021Quote: Self-association of GST-SP proteins was determined by native gel electrophoresis using Mini-PROTEAN TGX 4–20% precast polyacrylamide protein gels (Bio-Rad). Purified wild-type or mutant GST-SP (5 μg ...
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bioRxiv - Microbiology 2020Quote: All western blot samples were electrophoresed on Mini-PROTEAN TGX Stain-free 4-20% (w/v) pre-cast gels (Bio-Rad). Proteins were transferred to Immobilon-P membranes (EMD Millipore ...
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bioRxiv - Microbiology 2021Quote: ... proteins were denaturized in SDS-PAGE protein loading buffer for 5 minutes at 95°C and resolved by SDS-PAGE on 4-12% bis-tris Criterion XT Precast gels (Bio-rad), transferred to a PVDF membrane and blocked for at least 1h in 1% Casein blocking buffer (Sigma) ...
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bioRxiv - Microbiology 2021Quote: Lysates in the standard SDS sample buffer were heated and then resolved on a 4–20% SDS/PAGE gradient gel (Mini-PROTEAN, Bio-Rad), followed by electroblotting on a PVDF membrane via a Trans Blot Turbo (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... at 1.0 V/cm for ∼48 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). The buffer was changed every ∼24 hr.
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bioRxiv - Biochemistry 2020Quote: ... The protein purity was analysed by SDS-PAGE using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... following the manufacturer’s instructions and 12 µg of protein were loaded into 4-15% gradient gels (Biorad Miniprotean TGX, Biorad, Munich, Germany) and transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2021Quote: ... 5 µL of samples were loaded into a precast 4-20% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) alongside size standards (Precision Protein Plus ...
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bioRxiv - Immunology 2021Quote: 50 ug of protein was prepared in Protein Sample Loading Buffer (Li-Cor Biosciences) and loaded on Mini-PROTEAN 4-20% TGX Stain-Free Precast Gels (Bio-Rad). Immunoblotting was performed as detailed in the Supplementary Materials and Methods ...
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bioRxiv - Neuroscience 2021Quote: ... 10-50 μg of protein was loaded onto 4-15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2020Quote: Transfected cells were lysed in RIPA buffer on day 4 and proteins extracted were separated by 10% - 15% SDS polyacrylamide gel and transferred to Nitrocellulose membranes (Bio-Rad) at 100V for 1 hour and blocked for 1 hour with 5% non-fat milk (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... against the OAD buffer for 6h at 4 °C to remove the sucrose and then loaded on an EnrichQ 5/50 column (Bio-Rad) equilibrated with the ion-exchange buffer A (50 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... Fractions containing IADs and OAD were determined by SDS-PAGE (4-20% Mini-PROTEAN TGX precast protein gels run in SDS buffer (Bio-Rad)) ...
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bioRxiv - Physiology 2021Quote: ... and 30 μg of protein per sample was electrophoresed by SDS PAGE on 4–20% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad) and transferred to polyvinylidene fluoride membranes (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content was measured by Bradford assay and 30 mg of protein per sample were loaded into 4-20 % precast SDS-PAGE gels (Bio-Rad) under denaturing conditions ...
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bioRxiv - Physiology 2020Quote: ... Twelve micrograms of protein were subjected to SDS-PAGE on 4-20% Criterion TGX stain-free gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene diflouride membranes (PVDF ...
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bioRxiv - Biochemistry 2020Quote: ... After 24 h incubation at 4 °C the chelating agent was removed using a desalting column (Econo-Pac 10DG from Bio-Rad), pre-equilibrated with 20 mM Hepes buffer ...
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bioRxiv - Developmental Biology 2021Quote: Proteins extracted from Padi4CTL and Padi4CKO were subjected to SDS–PAGE (4–20% Mini-PROTEAN® TGX™ Precast gel, Biorad) and then transferred onto a nitrocellulose membrane ...
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Production and composition of group B streptococcal membrane vesicles varies across diverse lineagesbioRxiv - Microbiology 2021Quote: ... MVs (1.5 μg) were concentrated into a single band in a 4-20% Tris-Glycine SDS-PAGE gel (BioRad, Hercules, CA) that was fixed and stained using colloidal Coomassie blue (22).
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... and increasing concentrations of NONO(53-312) were prepared and incubated at 4°C for 1 hour before loaded onto a Dot Blot apparatus (Bio-rad) containing a nitrocellulose membrane (top ...