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Citations for Bio-Rad :
4101 - 4150 of 4995 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... before heating at 95°C for 5 minutes and finally loaded onto 4-20% polyacrylamide gel (Bio-Rad, Cat # 4561094). Samples were then transferred to a PVDF membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Samples were denatured in Laemli Buffer and boiled for 10 minutes then run on a 4-20% Criterion TGX precast midi protein gel (BioRad) and transferred to a PVDF membrane (Millipore Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then cleared by centrifugation for 15 minutes at 14,000 rpm at 4°C and subjected to immunoprecipitation with anti-V5 mouse monoclonal antibody (#MCA1360, Bio-Rad) overnight at 4°C with rotation in DNA/RNA LoBind tubes ...
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bioRxiv - Plant Biology 2024Quote: ... the indicated total protein amount was loaded on SDS-PAGE gels (4-20% Mini-PROTEAN TGX precast gel; Bio-Rad) and blotted either on PVDF Immobilon-P (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... and 10-20 μg of total protein were separated by SDS-PAGE using a 4-20% gradient (Bio-Rad 4561094), transferred to polyvinylidene difluoride membranes (EMD Millipore IPFL00010 ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein were loaded and resolved by polyacrylamide gel electrophoresis (PAGE) using Criterion TGX 4–20% Precast Gels (Biorad) and transferred onto PVDF membrane using iBlot gel transfer system (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... equal amounts of protein (50µg/lane) were loaded onto 4-20% gel electrophoresis of mini-protein TGX precast (Cat. #4561094, BIO-RAD) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... Four micrograms of protein/sample were loaded into 4%– 20% Mini-PROTEAN TGX stain-free polyacrylamide gels (Bio-Rad; 4568093), transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Cellular lysate was prepared from each samples and an equal amount of protein was loaded into a single lane of the 4-15% SDS-polyacrylamide gel (BioRad). After electrophoresis and transfer to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2024Quote: ... 20 μl of the protein sample was loaded on 4-20% Criterion TGX Gels (Bio-Rad Laboratories, Hercules, CA, USA). Gels were transferred onto 0.2 μm nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... on 4-20 % Mini-PROTEAN TGXTM Stain-Free Protein Gels (Bio- Rad) and 1x Tris/Glycine/SDS buffer (Bio-Rad). Proteins were then transferred onto a Trans- Blot TurboTM Mini PVDF Transfer membrane (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The lysates were then subjected to electrophoresis on 4%-20% gradient Mini-PROTEAN TGX precast gels (Bio-Rad Laboratories, 4561093) for resolution of the proteins ...
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bioRxiv - Plant Biology 2024Quote: ... Ten microliters of isolated total protein were separated on a 4-20% Tris-glycine stain free polyacrylamide gel (Bio-Rad) at 170 V for one hour in 1X Tris/glycine/SDS running buffer ...
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bioRxiv - Physiology 2024Quote: ... 4-HNE-conjugated protein bands between 100-25 kDa were visualized by the chemiluminescence imaging system (Chemi-Doc, Bio-Rad). Membranes were stained with Ponceau S dye to verify equal loading and transfer of proteins.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... equal amounts of protein (20µg/lane) were loaded onto 4-20% gel electrophoresis of mini-protein TGX precast (Cat. #4561094, BIO-RAD) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2024Quote: ... 4-degree Celsius) and the protein concentration in the supernatants was quantified using the DC protein assay (BIO-RAD, 5000116). Concentration was normalized before Western blot analysis ...
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bioRxiv - Biophysics 2024Quote: ... A total of 15 µg of protein was loaded into a 4-20% TGX 10-wells gel (Bio-Rad, USA) and the transfer step was performed using a Trans-Blot Turbo transfer system (Bio-Rad ...
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Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexesbioRxiv - Biophysics 2024Quote: ... Gel electrophoresis was performed in ice-cold 0.5× TBE buffer (4 °C) and images were captured on a ChemiDoc MP™Imaging System (BioRad). Quantification was performed using ImageLab (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... Protein samples were separated by SDS-PAGE using Mini-PROTEAN TGX Stain-Free Precast 4-15% Gels (Bio-rad; 4568083) and transferred onto a PVDF membrane (Bio-rad ...
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bioRxiv - Bioengineering 2024Quote: ... cDNA synthesis was performed using 0.5 µg of total RNA and 4 µl of iScript RT Supermix (Bio-Rad™) in a thermocycler ...
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bioRxiv - Biochemistry 2024Quote: Protein purity was checked by SDS-PAGE using 4-15% Mini-PROTEAN® TGX™ precast protein gels (Bio-Rad). Proteins were diluted in 2x SDS-PAGE loading buffer reaching a final concentration of 100 mM DTT or in DTT-free buffer ...
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bioRxiv - Biochemistry 2024Quote: ... bacteria were pelleted and 10-40 µl bacterial supernatant was run on an SDS-PAGE MiniPROTEAN TGX stain-free 4-20% gel (BioRad) and blotted onto a polyvinylidene difluoride membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... 15-20 µg of protein was separated on 7.5% or 10 or 12% acrylamide gels or on 4-20% Mini-PROTEAN TGX Precast Gels (4561096, Bio-Rad) by SDS-PAGE ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction products were then resolved by SDS–PAGE on a 4–20% gradient gel and transferred to a nitrocellulose membrane (BioRad). The membrane was blocked with 2.5 % BSA in TBST for 1 hour at room temperature with gentle rocking ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins (10-15 μg) were resolved on 4-15% SDS-PAGE gels and transferred onto nitrocellulose membranes (Bio-Rad Laboratories). Membranes were blocked in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were incubated overnight at 4°C with anti-F4/80 antibody (F4/80 antibody | Cl:A3-1, Bio-Rad, USA). Endogenous peroxidases were quenched by incubating the sections in 3% hydrogen peroxide (Acros Organics ...
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bioRxiv - Cell Biology 2024Quote: ... BioRep Diabetes) by sandwiching them between two layers of hydrated Bio-Gel P-4 polyacrylamide beads (150-4124, Bio-rad). These chambers were attached to a high-precision eight-channel dispenser pump (ISM931C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were resolved by SDS–PAGE under denaturing conditions using 4- 20% Mini-PROTEAN® TGXTM precasted polyacrylamide gels (Biorad). Proteins were transferred to nitrocellulose membranes (Biorad) ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were rinsed 3×10 minutes in TBST 0.1% then incubated with appropriate secondary antibody coupled to the horseradish peroxidase (Biorad, 1706515, 1706516; 1/5000) for 1 hour at room temperature under agitation ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were washed 3 times 5 min with washing solution and incubated 1 h with anti-rabbit IgG HRP-conjugated secondary antibodies (Bio-Rad, 1:3000) at room temperature ...
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bioRxiv - Systems Biology 2021Quote: Differential expression of ten selected transcripts in response to virus at 3-L1 stage was examined using Droplet Digital PCR (ddPCR) (Bio-Rad, Hercules, CA). Because of the difficulty in obtaining a large amount of gut RNA samples and the limited sensitivity of conventional qPCR technique to detect gene expression at a low level ...
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bioRxiv - Cell Biology 2021Quote: ... Band density was normalized according to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) content and analyzed using Image Lab software (Bio-Rad, California, USA) (29).
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bioRxiv - Plant Biology 2021Quote: ... Membranes were washed 3 x 10min in TBS + Tween 20 before proteins were detected by Immuno-Star luminol-peroxide (Bio-Rad, #170-5070). Bio-Rad Chemi-doc was used to visualize and quantify proteins with densitometry.
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 µg) from each sample sets (n=3) was taken for cDNA preparation using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Real-Time qPCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... incubated with 3% H2O2 for 15 min at room temperature and then incubated with goat anti-rabbit IgG-HRP (Bio-Rad 170-6515) secondary antibody diluted 1:300 in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were denatured at 95 °C for 3 minutes prior to loading onto a 16.5% Mini-PROTEAN® Tris-Tricine Gel (Biorad Cat. No. 4563066). Gels were transferred to a nitrocellulose membrane (Millipore Sigma Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell suspension then was divided equally and heated for 3 min at indicated temperatures on T100™ Thermal Cycler (Bio-Rad Laboratories). Cells were snap-frozen and thawed twice and centrifuged at 17.000 g for 15 min at 4°C ...
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bioRxiv - Genomics 2024Quote: ... 4.5 µl of sample were used to generate single cell RNA-Seq libraries by following the Illumina Bio-Rad SureCell WTA 3’ Library Prep Guide (Illumina, San Diego CA and Bio-Rad, Hercules, CA) and using the ddSEQ Isolator for Single Cell Sequencing (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... Migration of the DNA in the gel was visualized via the fluorescent Cy-3 label (ChemiDoc™ MP Imaging System, BIO-RAD, USA). Protein-DNA complexes were transferred from the gel to the PVDF membrane (Immun-Blot® PVDF Membrane ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL primer 556R (5’ CTTTACGCCCARTRAWTCCG 3’) at 10 µM and 10 µL SYBER® Green Master Mix (Bio-Rad, Veenendaal, The Netherlands). The DNA extraction estimated an average rRNA yield corresponding to 5×10^8 bacterial cells/mL.
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bioRxiv - Microbiology 2023Quote: ... for the selected clinical isolates using the indicated concentrations of antibiotics via an alamar blue reduction assay completed as described but without shaking.8 After 3 days of incubation with the alamar blue reagent (BioRad, Hercules, CA, USA), the MIC was identified as the lowest concentration of drug that inhibited the reagent color change as determined visually.
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bioRxiv - Microbiology 2024Quote: ... and R-tot (5′-CTGCTGCCTCCCGTAGGAGT-3′) in the Rotor Gene 6000 thermocycler (Corbett Research, Sydney, Australia) with the iTaq Universal SYBR Green Supermix (Bio-Rad, Reinach, Switzerland).
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bioRxiv - Biophysics 2024Quote: ... His-tagged protein was bound to 3 mL HisPur™ Ni-NTA Resin (Thermo 88221) equilibrated with lysis buffer in a glass Econo-Column® (Bio-Rad) by flowing through the clarified lysate ...
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bioRxiv - Molecular Biology 2024Quote: ... 3-10 μg total protein was loaded and run on AnykD™ Criterion™ TGX™ Precast acrylamide protein gels (Biorad cat#5671125) then transferred to nitrocellulose (Biorad cat#1704159) ...
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bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
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bioRxiv - Cancer Biology 2021Quote: ... The same amounts of protein extracts were subjected to polyacrylamide gel electrophoresis using the 4% to 20% Mini-Protean TGX gel system (Bio-Rad), transferred to PVDF (0.45 μm pore size ...
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bioRxiv - Cancer Biology 2021Quote: Protein samples from LNCaP cells used to measure luciferase activity (described in Fig. 4A) were loaded into 4% to 15% SDS-PAGE (Bio-Rad) and subjected to electrophoretic analysis and subsequent blotting ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... 1.5 μl for bound franction for GFP detection) were loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.