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Citations for Bio-Rad :
4151 - 4200 of 4215 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... Percentage of targeted alleles within a cell population was measured with a Bio-Rad QX200 ddPCR machine and QuantaSoft software (v.1.7; Bio-Rad, Hercules, CA, USA) using the following reaction mixture ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells resuspended in PBS were transfected with 75μg DNA constructs (Gene Pulser Xcell System; Bio-Rad; 250 V, 500 μF, 0.4-cm cuvettes). Cells were subsequently plated on MEF feeder layers (DR4 MEFs for puromycin or neomycin selection ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were separated in 12.5 % sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE; 100V for 90 minutes) in Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad, Hercules, CA, USA) and transferred into polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2019Quote: ... Second-dimension sodium dodecyl sulphate-polyacrilamide gel electrophoresis (SDS-PAGE) was done on 12.5% Tris-HCL Precast Gels using a Criterion electrophoresis cell (Bio-Rad Laboratories, Hercules CA, USA), run at 80V for 20 min before switching to 100V for approximately 2 h ...
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bioRxiv - Bioengineering 2019Quote: ... Equal volumes of the cell suspension (RT) and the agarose solution (75°C) were mixed and immediately transferred to plug molds (Bio-Rad Laboratories, Cat. # 1703713), ∼100 μl mixture per plug ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 μg proteins of the cell line were exposed to a 10% SDS-PAGE gel and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). After blocking the membranes using 5% non-fat milk or bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... the cell lysates were separated by 15% SDS-polyacrylamide gel electrophoresis and electro-blotted onto a Immun-Blot PVDF Membrane using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 2% skimmed milk and probed with antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... or self-made gel and subjected to a sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) using a Mini-PROTEAN® Electrophoresis cell (Biorad, Cat No 1658002EDU). Gels were run at 130 V for approx ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of transcribed RNA were electroporated into 8×106 Vero cells using the Gene Pulser Xcell™ Electroporation Systems (Bio-rad, California, USA) under as described previously41 ...
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bioRxiv - Cancer Biology 2021Quote: Separation of 20 μg or 50 μg protein from cell lysate was performed on a 10% Mini-PROTEAN® TGX™ gel (BioRad, Portland, ME) and subsequently transferred onto Immunoblot PVDF 0.45μm membranes (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... Equal concentrations of samples (15μg for whole cell lysates and 5μg for nuclear fractions) were resolved on Criterion gels (Bio-Rad, catalog number 5671093 and 5671094) and transferred to nitrocellulose (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... protein extracts were obtained from mock-or MAYV-infected HeLa cells or HDFs that were treated or untreated with PIM kinase inhibitors in Laemmli buffer with 10% Dithiothreitol (Bio-Rad, Hercules, CA, USA). Proteins were separated in SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were solubilized in Laemmli sample buffer and heated at 95°C for 10 min to denature proteins and separated via SDS-PAGE for 40–60 min at 270 V using 4–20% stain-free gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Thereafter ...
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bioRxiv - Cancer Biology 2022Quote: Equal amounts of cell lysate were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Blocking was performed for 60 min with 5% nonfat dry milk in Tris-buffered saline and Tween 20 ...
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bioRxiv - Neuroscience 2021Quote: ... QPCR was performed to determine Piezo2 knockdown effects by transfection of piezo2-shRNA into N2A cells using IQ Syber Green supermix (Bio-rad, Hercules, CA, USA) on a Bio-rad CFX96 Real-time PCR Machine and specific intron-spanning primers to quantify the cDNA levels of Piezo2 (Table 1) ...
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bioRxiv - Cell Biology 2019Quote: Sequence-confirmed constructs were linearized and transformed into the macronucleus of WT cells by biolistic bombardment using a PDS-1000 particle bombarder (Bio-Rad, Hercules, CA, USA) (Bruns and Cassidy-Hanley ...
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bioRxiv - Neuroscience 2021Quote: A multiplex biometric immunoassay containing fluorescent dyed microbeads was used to measure cytokines in the cell culture supernatant (Bio-Rad Laboratories, Hercules, CA, USA). The following cytokines were quantified ...
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bioRxiv - Cancer Biology 2019Quote: ... for approximately 45 min using the Mini-PROTEAN Tetra Cell and PowerPac™ 300 using tris-glycine SDS running buffer (all Bio-Rad, Watford, UK). Protein ladder SeeBlue™ Plus 2 Pre-stained Protein Standard (Invitrogen™ through Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The resuspended cDNA was divided into six aliquots and each aliquot was transformed into ElectroMax™ DH10B™ T1 phage resistant competent cells in a separate vial using a Gene pulsor Xcell™ electroporation system (Bio-Rad) as mentioned in the kit’s manual ...
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bioRxiv - Biochemistry 2020Quote: ... Native-SDS PAGE was carried out as follows: samples were subjected to a Bio-Rad Protean II xi slab cell (Bio-Rad, Richmond, CA, USA), with a discontinuous buffer system (Laemmli ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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bioRxiv - Neuroscience 2022Quote: We prepared cellular extracts by a single-step lysis method.75 We suspended harvested cells in SDS-PAGE sample buffer [Laemmli sample buffer (Bio-Rad, Cat# 161-0737)] before boiling for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on 10%–12% SDS polyacrylamide gels by electrophoresis and transferred onto nitrocellulose membranes using a Trans-Blot SD Semi-Dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% skim milk for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... Separation in the second dimension was accomplished using an 10% SDS-PAGE in a Protean II xi 2-D Cell (Bio-Rad, Hercules, CA, USA) at 50 mA until the bromophenol blue reached the bottom of the gel ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred from polyacrylamide gel to polyvinylidine difluoride (PVDF) membranes as described by (Towbin et al., 1979) using a Trans-blot® electrophoretic transfer cell (Bio-Rad, city, country). The PVDF membrane with transferred proteins was blocked overnight at 4°C in blocking solution (5% (w/v ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Microbiology 2021Quote: ... 50 μl of cells were mixed with PCR products and transferred to a precooled 2 mm gap electroporation cuvette (Bio-Rad Laboratories, Hercules, CA). A BTX Gemini X2 instrument was used for electroporation with the following settings ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins or whole cell lysates were separated by SDS-polyacrylamide gel electrophoresis on a 4-20% gradient gel (Bio-Rad Laboratories, Hercules, CA) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2021Quote: We mixed 500 μg of extracted cell lysates and 100 μL of patients’ sera with 100 μL of SureBeads™ Protein G Magnetic Beads (Bio-Rad, Hercules, CA) and added phosphate-buffered saline containing 0.2% of Tween 20 (PBS-T ...
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bioRxiv - Immunology 2020Quote: ... The cellular localization of EqPD-1-EGFP and EqPD-L1-EGFP was then confirmed using the ZOE Fluorescent Cell Imager (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Immunology 2020Quote: Staining of 2×105 cells per well was performed with mouse anti-human CD79α (clone HM57, Bio-Rad AbD Serotec, Puchheim, Germany, 1:100) for identification of B cells and Alexa Fluor 647-conjugated rat anti-human CD3∊ (clone CD3-12 ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl were loaded and resolved on 6% sodium dodecyl sulfate (SDS) gels and transferred onto nitrocellulose membranes with Mini-PROTEAN III and Mini Trans-Blot cells (Bio-Rad, Hercules, CA, USA), respectively ...
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bioRxiv - Microbiology 2022Quote: ... the red fluorescent intensities of the microchambers on each device were examined to determine the MIC using a fluorescent microscope (ZOE™ Fluorescent Cell Imager system; Bio-rad, Hercules, California). For slow growth samples where the MIC was not clear after 4 h incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... and transferred to PVDF transfer membrane (IPVH00010 Immobilon®-P; MilliporeSigmaTM, Burlington, USA) overnight at 10 mA in a cold room (Mini trans-blot cell; Bio-Rad, Hercules, USA). The membrane was blocked for non-specific sites and incubated with anti-HvHsfA6a in 1:2000 dilutions overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated on an 8-12% SDS-polyacrylamide gel and blotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), The membrane was incubated overnight at 4°C with primary Abs ...
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bioRxiv - Biochemistry 2023Quote: ... Total protein concentrations of the cell extracts were determined using the Bio-Rad Protein Assay Kit according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA) using BSA as standard.
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bioRxiv - Cell Biology 2023Quote: ... the protein was transferred to the 0.2 μm nitrocellulose membrane using a semi-dry transfer system (#1703940, Trans-Blot® SD Semi-Dry Transfer Cell, Bio-Rad, Hercules, CA, USA) for 55 min at 25 V using 1x transfer buffer (Pierce Western Blot Transfer Buffer 10x ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were obtained from MAYV- or UNAV-infected HMC3 or HBEC-5i cells in Laemmli buffer with 10% dithiothreitol (Bio-Rad, Hercules, CA, USA). Proteins were fractionated in SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: IRX4_PEP1 transfected PC3 cell protein lysate was pre-cleared with 25 μL of SureBeads TM Protein G magnetic beads (BIO-RAD, Catalog number – 1614023) for 1 hour at 4 ºC and the beads were removed three times using a magnetic stand ...
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bioRxiv - Biophysics 2023Quote: ... then transferred onto PVDF membrane (Hybond-LFP, Amersham BioSciences, Little Chalfont, Buckinghamshire, United Kingdom) by using a semi-dry blotter (Trans-Blot Cell, Bio-Rad, Hercules, CA, USA). To differentiate the truncated product from T2 ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μg of dsDNA were added to the competent cells and then transferred to chilled 2 mm gap electroporation cuvettes (Bio-Rad Laboratories GmbH, Germany). Electroporation transformation was done with a single pulse at 1.8 kV ...
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bioRxiv - Developmental Biology 2023Quote: ... resolved by SDS-PAGE and transferred to PVDF membranes in a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad, Hercules, CA, USA). Unspecific binding to membranes was prevented by blocking in 5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples containing 30 μg of total protein were separated by SDS-PAGE for 30 min at 270 V using 4–20% gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Bioengineering 2023Quote: ... was subjected to SDS-PAGE using a 4-20% Mini-PROTEAN TGX acrylamide gel on a Mini-PROTEAN Electrophoresis cell (Bio-Rad Laboratories, CA, USA). Electrophoretically separated proteins were transferred to Immobilon-P PVDF membrane (25V/RT/overnight) ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was used as cell extract for further experiments after determination of the protein concentration using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... Insert DNA was derived from total RNA extracted from U2OS cells and then reverse transcribed using an iScript™ Reverse Transcription Supermix (Bio-Rad Laboratories, Hercules, CA). A 140 bp region of alpha satellite sequence from chromosome 4 and a 349 bp region of HSATII from chromosome 7 were independently cloned into pTargeT™ DNA backbone via StrataClone TA cloning (Agilent Technologies ...
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bioRxiv - Bioengineering 2019Quote: Free FAM NHS ester remaining after purification was quantified by polyacrylamide gel electrophoresis (PAGE) run according to the Laemmli protocol51 (adapted in BioRad Mini-PROTEAN Tetra Cell manual). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed in a Bio-Rad Criterion TM Vertical Electrophoresis Cell at 90-120V for 1-2 hours and then transferred to a PVDF Transfer Membrane (#88518, Bio-Rad, Hercules, CA, United States) using the Bio-Rad Trans-Blot Turbo Transfer System ...