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Citations for Bio-Rad :
3901 - 3950 of 4995 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... total protein lysates of T.brucei BSF were separated by SDS-PAGE (4-20% Mini PROTEAN TGX stain-free precast gradient gels, Bio-Rad) and blotted on PVDF filters (Bio-Rad) ...
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bioRxiv - Developmental Biology 2022Quote: ... P14 and P18 retinas were measured by fluorescent Western blot analysis [4-15% or 7.5% Mini-PROTEAN TGX gels (Bio-Rad)] and quantitation was performed using the LICOR (LICOR ...
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bioRxiv - Biochemistry 2022Quote: ... protein samples were separated by precast 4–15% Criterion™ TGX Stain-Free™ Protein Gels (Bio-Rad Laboratories 5678085), transferred to a nitrocellulose membrane using Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs (Bio-Rad Laboratories 1704159EDU ...
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bioRxiv - Cell Biology 2022Quote: ... The whole cell lysate and crude cytoskeletons were resolved on 4-20% Mini-PROTEAN tris-glycine polyacrylamide gels (Bio-Rad), and Western blots were stained with 1:5000 diluted anti-beta-actin mouse monoclonal antibody (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed with IP lysis buffer three time and boiled in 25 ul of 2X SDS-loading buffer for 5 minutes and loaded into 4-15% polyacrylamide gels (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... 15-35 µg protein were loaded and separated by SDS-PAGE in 4–20% Mini-Protean TGX Precast gels (BioRad). Gels were transferred to PVDF membranes using the iBlot system (Thermo) ...
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bioRxiv - Cell Biology 2022Quote: ... 15 ug of mitochondrial proteins or 25 ug of total cellular proteins were separated by gel electrophoresis on 4-20% gradient TGX gels and transferred onto PVDF or nitrocellulose membranes using Trans-Blot Turbo Transfer system (BioRad). Mitochondrial electron transport chain proteins were detected using Total OXPHOS Rodent WB Antibody Cocktail (ab110413) ...
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bioRxiv - Biochemistry 2020Quote: ... 750 ng of wtXLF and each tandem dimer construct were loaded and run on a 4-15% Mini-PROTEAN TGX Precast Protein Gel (BioRad). tdXLF constructs where the KBM of subunit 1 was replaced with additional linker sequence (lane 4 tdXLFΔKBM/WT and lane 6 tdXLFΔKBM/ΔKBM ...
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bioRxiv - Microbiology 2020Quote: ... Protoplast concentration was adjusted to 4 × 108 cells/ml and added to the same amount of 1.2% low melting agarose gel (Bio-Rad) solution ...
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bioRxiv - Neuroscience 2020Quote: ... 0.02% bromphenol blue) for 15min at 50°C and 5μg of protein were separated on a 4-20% SDS PAGE (Biorad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Developmental Biology 2020Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Plant Biology 2021Quote: ... 10 µl PCR reactions were performed in technical duplicate with SensiFAST SYBR No-ROX (Meridian Bioscience) with 4 ng cDNA and 300 nM primers (Table S1) on a CFX96 Real-time PCR System (BioRad). Mean PCR reaction efficiencies were calculated for each primer pair with LinRegPCR (Ruijter et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... and adjusted before isolation of protein aggregates as well as verified by running reduced samples on Criterion XT Precast Gel 4-12% Bis-Tris (BioRad) and staining with Coomassie Brilliant Blue R-250 staining solution (BioRad) ...
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bioRxiv - Physiology 2021Quote: ... Approximately 10 µg of protein was loaded into a 4% - 20% Criterion pre-cast gel (Bio-Rad, Hercules, CA, USA) and resolved at 120 V for 120 min ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... and then loaded without prior heating on a 4-20% gradient polyacrylamide gel and separated by denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (BioRad). The gel was exposed to a PhosphorImager screen in a cassette containing an enhancing screen ...
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bioRxiv - Cancer Biology 2021Quote: ... centrifuged for 20 minutes at 13,000 rpm at 4°C and the protein concentration in the supernatant was measured with an DC protein assay (BioRad). Samples were separated by SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed and then precipitated in 0.1 M glycine pH 3.5 and neutralized with 0.5 M Tris?Cl and 1.5 M NaCl pH 7.4 before SDS-PAGE electrophoresis (8%, at 100 V for 90 min) followed by transfer to a PVDF membrane (BioRad). Membranes were washed in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2020Quote: ... An equal amount of total protein (30μg) were resolved on precast 4-12% SDS-PAGE gels (Biorad, Hercules, CA, USA), transferred onto nitrocellulose membranes ...
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Sparcl1/Hevin drives inflammatory and neuropathic pain through astrocyte and NMDA receptor signalingbioRxiv - Neuroscience 2022Quote: ... and 30 μg of proteins were loaded for each lane and separated on SDS-PAGE gel (4–15%; Bio-Rad). After the transfer ...
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bioRxiv - Neuroscience 2022Quote: ... heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (4 –15% Tris-HCl; BioRad) and transferred onto Immun-Blot PVDF 0.2 µm (BioRad ...
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bioRxiv - Physiology 2022Quote: ... Total protein (30 μg) per lane was separated by SDS-PAGE using 4–15% Tris-glycine gels (Bio-Rad Laboratories) and transferred to PVDF membrane (Millipore Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... and equivalent amounts of protein (∼30 μg) were separated by SDS-PAGE (1.0 mm thick 4 to 12% [w/v] gradient polyacrylamide Criterion Bio-Rad) in MOPS buffer (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: ... The soluble protein extracts were separated by SDS-PAGE (4-15% mini-protein TGX precast gel, 12 or 15 well, BioRad) and transferred to a PVDF membrane (Trans-blot Turbo mini-Format 0.2 μM PVDF ...
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bioRxiv - Neuroscience 2022Quote: ... protein samples (10-30 μg) were boiled and separated on precasted 4-20% Criterion TGX Stain-free gels (Bio-Rad) and transferred to a nitrocellulose membrane (Amersham Protran 0.2um NC ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Equal amounts of protein were run on 4 – 15% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad, USA) followed by blotting to a Polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded in precast 4-20% gradient sodium dodecyl sulfate (SDS) polyacrylamide gels (Mini-PROTEAN TGX gels, Bio-Rad) and run at room temperature with 100 V (constant ...
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bioRxiv - Cell Biology 2020Quote: Nucleosome fractions containing 200 ng DNA were applied for SDS-PAGE with 4-20 % gradient SDS-PAGE gel (Bio-rad). Proteins were transferred to the nitrocellulose membrane (GE ...
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bioRxiv - Genetics 2020Quote: Equal volumes of lysate from each fraction were separated by SDS-PAGE using 4-20% TGX Stain-Free mini gels (BioRad). Protein was transferred to PVDF membrane using the TransBlot Turbo system (BioRad) ...
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bioRxiv - Biophysics 2020Quote: ... Western blot assays were performed after resolving the samples on a 4-15 % Tris-HCl gradient gel (Bio-Rad Laboratories).
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bioRxiv - Bioengineering 2020Quote: ... Specific volumes of samples (described previously) were loaded on a 4−20 % Mini-PROTEAN-TGX gel (BioRad, Hercules, CA, USA) and run at 180 V for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
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bioRxiv - Physiology 2020Quote: ... Ten micrograms of protein were subjected to SDS-PAGE on 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad) and transferred to polyvinylidene diflouride (PVDF ...
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bioRxiv - Physiology 2020Quote: ... equal amounts of protein (20 ug) were loaded in 4-12% - Criterion XT Bis-Tris protein gels (Bio-Rad, CA) and transferred to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
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bioRxiv - Systems Biology 2021Quote: ... and 6 μl for slow growing mutants with lower OD600) on Stain-Free gels (4-20%, CAT # 4568096, Bio-Rad, Tris/Glycine SDS Buffer (CAT # 161-0732 ...
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bioRxiv - Physiology 2021Quote: ... we ran the purified Hb fractions on a mini-protean precast 4-20% SDS PAGE gel (Bio-Rad, Hercules, CA), which we then stained with Coomassie brilliant blue-G ...
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bioRxiv - Physiology 2021Quote: ... were handpicked to match size of islets across the samples and loaded into the polycarbonate perifusion chamber between two layers of polyacrylamide-microbead slurry (Bio-Gel P-4, BioRad) by the same experienced operator ...
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bioRxiv - Microbiology 2020Quote: ... The samples were heated at 90°C for 10 minutes and loaded onto a 4-20% precast polyacrylamide gel (BioRad). The gel was stained with SimplyBlue SafeStain (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 20 μL of the total crude cell lysate from each sample was loaded onto a 4–20% gradient Tris-HCl Criterion SDS-PAGE (BioRad). After transfer to polyvinylidene fluoride membrane ...
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bioRxiv - Biophysics 2021Quote: ... Purified TREK-2 was then mixed with GUVs to a final concentration of ∼1 - 5 µg/ml and incubated overnight at 4 °C with 0.5mg/ml Bio-Beads (Bio-Rad) prior to use.
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bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad). PVDF membranes were used for proteins wet transfer (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... 10μg/μl of protein was loaded into each lane of 4–15% (mg/100 mL) Tris·HCl polyacryamide gels (Bio-Rad). Protein was transferred to Immobilon-P PVDF membranes (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation at 10,000xg for 10 minutes at 4°C and then boiled in 2x Laemmli Sample Buffer (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: Eluted samples were resolved by SDS-PAGE using 4-20% gradient gels and then transferred to the nitrocellulose membrane using the Trans-Blot Turbo transfer system (Biorad). Following the blocking for 1 hour in blocking buffer (5% milk in TBST) ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed with the washing solution twice and then reacted with 4-chloro-1-naphthol (Bio-Rad Laboratories) in the HRP color development buffer (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... two probes (250 nM final each) (Supplementary Table 4) and template DNA were combined with ddPCR Supermix for Probes (No dUTP) (BioRad) in a final 20 μL reaction volume ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μg of protein was loaded on 4-20% Mini-PROTEAN TGS Precast Protein Gels (Cat. No. 4561094, Bio-Rad) from each condition and ran at 100 V for 1 hour followed by transferring the protein on a PVDF membrane ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were then loaded (15-20 μg of protein per well) in a 4–20% Precast Protein Gel (Biorad) and resolved by SDS-polyacrylamide gel electrophoresis ...