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Citations for Bio-Rad :
3951 - 4000 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... Appropriate total proteins from different lines were resolved on 10 % polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-Rad). Anti-FLAG monoclonal antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... flow through and fractions from the peak were run on an a 10% SDS-PAGE (Bio-Rad Cat # 345-0010) to confirm purity and determine which should be pooled ...
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bioRxiv - Molecular Biology 2021Quote: ... We denature the lysates and resolve using 10% (vol/vol) SDS/PAGE before blotting on PVDF membrane using a TransBlot Turbo (BioRad). Blots are incubated with antibodies against SOD1 (Sigma SAB5200083 ...
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bioRxiv - Neuroscience 2021Quote: ... The whole extracts (20 µg) were loaded on 10% SDS-PAGE gel and blotted on PVDF (Polyvinylidene fluoride) membrane (Biorad) with wet apparatus (Biorad) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was precipitated onto 10-micron gold carriers for biolistic bombardment of the macronucleus using a Gene Gun (Bio-Rad). For disruption of Q22YU3/SHULIN (TTHERM_00122270 ...
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bioRxiv - Molecular Biology 2021Quote: ... The dried pellets enriched for histones were dissolved in sample buffer (8 M urea, 5% β-mercaptoethanol and 10 mM Tris-HCl (pH 7.0)) and added sample loading buffer (Biorad XT sample buffer ...
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bioRxiv - Molecular Biology 2020Quote: Whole-cell lysates were run on 10 or 15% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (1620097, Bio-Rad) by standard methods ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to 0.2 µm nitrocellulose membrane on a semi-dry transblot (Trans-Blot Turbo, Bio-Rad). The membrane was blocked with 5% BSA (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Polyacrylamide gels used in this study were either prepared using 10% EZ-Run protein gel solution (Fisher) or precast gradient gels (4-20%, from Biorad). Any quantification performed on western blots was done using ImageJ software ...
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bioRxiv - Biochemistry 2019Quote: ... 20 μg was separated by electrophoresis in 10% polyacrylamide gels and transferred to polyvinylidene difluoride membranes using Trans-Blot Turbo Transfer System (Biorad). After 1 h incubation at room temperature in Odyssey Blocking Buffer in TBS (Licor) ...
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bioRxiv - Microbiology 2019Quote: ... YpdA and YpdAC14A proteins were pre-reduced with 10 mM DTT followed by DTT removal with Micro Biospin 6 columns (Biorad). For the biochemical activity assays of the specific BSSB reductase activity ...
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bioRxiv - Genetics 2020Quote: ... Samples were centrifuged at 17,000 × g for 30 min at 4 ºC and the supernatant was run on a 10% Mini-PROTEAN TGX precast polyacrylamide gel (BioRad). Protein was transferred to a PVDF membrane using the iBlot 2 (Life Technologies) ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were then loaded onto an SDS-PAGE gel (10% acrylamide) and transferred to a nitrocellulose membrane using a Trans-blot Turbo System (BioRad), using a 30 minute program of 25V ...
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bioRxiv - Biochemistry 2019Quote: Trypanosome whole cell protein lysates (2×106 cells per well) were separated using SDS-PAGE (10% gels) and transferred by semi-dry blotting (BioRad) for 45 min at 25 V to PVDF membranes ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples (10 µL) were run on 12.5% SDS-PAGE gels along with a Precision Plus Protein dual colour standard (Bio-Rad) and transferred to PVDF membranes ...
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bioRxiv - Plant Biology 2020Quote: ... Denatured protein extracts were separated by SDS-PAGE on 10% TGX Stain-Free™ Fast-Cast™ gels (Bio-Rad). Separated proteins were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of proteins (30-60µg) were separated via SDS polyacrylamide gel electrophoresis (SDS-PAGE) using precast 10% polyacrylamide gels (Mini-Protean TGX, Bio-Rad). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were boiled at 95 °C for 10 mins with 6×SDS sample buffer before loading onto 4–20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Physiology 2020Quote: ... Equal amounts of protein were resolved on SDS-PAGE (10% Mini-PROTEAN® TGX Stain-Free™ Gels, Bio-Rad) and transferred to polyvinyl difluoride membranes (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... in TE (25 min) followed by de-staining in TE (5-10 min) and imaging on a ChemiDoc XRS+ instrument (BioRad). The fraction of nucleosomal DNA shifted was quantified using Image Lab 6.0.1 (BioRad ...
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bioRxiv - Immunology 2019Quote: ... and 500 ng protein was loaded to 10% TGX (Tris-Glycine eXtended) Stain-Free FastCast polyacrylamide gels (Bio-rad, # 1610183), 200 V running for 45min ...
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bioRxiv - Physiology 2019Quote: ... initiation at 95°C/10 s followed by 45 amplification cycles (60°C/15 s) on the CFX Thermal cycler (Biorad).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... equal amounts of protein were subjected to electrophoresis on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). Membranes were treated with antibodies using standard Western blot techniques ...
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bioRxiv - Biochemistry 2021Quote: ... 15 μg of proteins were loaded on a 10% Tris–glycine precast SDS-PAGE gel (Mini-PRO-TEAN TGX, BioRad). After a short migration (0.5 cm ...
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bioRxiv - Genetics 2020Quote: ... The reaction was incubated at 37°C for 15 min and stopped by the addition of 10 μl of TBE-UREA-sample buffer (BioRad). The samples were analysed on a 15% denaturing polyacrylamide gel in 1 × TBE buffer.
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bioRxiv - Cancer Biology 2021Quote: ... DNA was diluted to 10 ng/µland 2 µl was used in each RT-qPCR reaction with 10 µl iTAQ Universal SYBR green supermix (BioRad) and 400 nM primers ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred onto 0.2 µm pore nitrocellulose membranes for 10 min at 2.5 V using the Trans-blot turbo transfer system (Bio-Rad). For more sensitive detection of neurexin levels ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... The 20 μL reaction mix contained 5 μL sample or standard DNA together with 10 μL 2x SsoAdvanced Universal Probes Supermix (Biorad), 1 μL of each primer (5 μM stock solution) ...
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bioRxiv - Bioengineering 2020Quote: ... Then membrane was then placed inside a transparency and exposed 10-30 s on a ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Biochemistry 2021Quote: ... Mixtures were heated to 90°C for 10 min and slowly cooled to 40°C (0.1°C / second) using a Dyad PCR machine (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad). Proteins were then transferred onto low-fluorescence 0.45 μm PVDF membranes with transfer buffer (10× Tris/glycine with 10% methanol ...
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bioRxiv - Neuroscience 2021Quote: Samples were resolved by SDS-PAGE using either 10 or 12% acrylamide/bis-acrylamide gels and transferred to 0.22 µm nitrocellulose (Bio-Rad). Membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated for 10 minutes before measuring absorbance at 595 nm on BioRad-Smart Spec plus spectrophotometer (BioRad, USA). Protein concentration was calculated against a standard curve drawn using serial dilutions of BioRad BSA standard solution.
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were separated by 10% SDS-PAGE followed by electrophoretic transfer to polyvinylidene difluoride membranes (Bio-Rad, Hercules CA). Membranes were blocked with 5% skim milk in PBS containing 0.1% Tween 20 and incubated at 4°C overnight with the TRPM4 antibody ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was loaded in a 10-well 4-20% Mini-PROTEAN TGX Precast protein gel (BIO-RAD, Catalog #4561094). The gel was run at 150V for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: Samples were separated on a 10% SDS-PAGE gel and transferred to a nitrocellulose membrane using a semi-dry transfer (Biorad). The membrane was dried ...
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bioRxiv - Plant Biology 2020Quote: ... The gel blot was done as described before (Loizeau et al., 2014): 10 μg total RNA was separated on 15% polyacrylamide TBE urea gels (Biorad) and transferred in a wet-blot setup with 0.5 TBE buffer to a Hybond-N membrane (GE-Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... self-cast 10% or 12% SDS-gels or Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad). After electrophoretic separation ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then resuspended in sample buffer and run on a 10% polyacrylamide pre-cast gel (Bio-Rad, Hercules, CA) for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Microbiology 2021Quote: ... The equivalent volume to 10 μg of protein was run in a Stain-Free FastCast 12.5% polyacrylamide gel (Bio-Rad) and transferred to a nitrocellulose membrane using the Trans-Blot Turbo semi-dry system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Onboard de-waxing was performed in accordance with the standard Leica Bond protocol and staining undertaken using IHC Protocol F with the following adaptations: additional non-specific block prior to primary antibody incubation (10% normal horse serum (Biorad), 1x Casein (Vector Labs ...
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bioRxiv - Plant Biology 2021Quote: ... The IEF was performed on the 7cm length gel strips with immobilized pH gradients 3-10 and 3-6 (BioRad). After IEF ...
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bioRxiv - Plant Biology 2021Quote: ... 20 µL of each sample was loaded and the proteins were resolved using 10% SDS-PAGE resolving gel followed by transfer to a polyvinylidene difluoride membrane (BioRad) at 100 mA for 12 h ...
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bioRxiv - Molecular Biology 2020Quote: ... The labeled GyrA14-I491C was loaded onto a pre-equilibrated CcdB affinity column prepared using Affi-gel 10 (Bio-Rad), and incubated at 4°C for 4 hours on a rotary mixer to enable binding ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were in-gel rehydrated for 16 hrs and isoelectrically focused on 7 cm pH 3-10 IPG strips to 10,000 Vh on a Protean® IEF Cell (BioRad). After focusing ...
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bioRxiv - Cancer Biology 2020Quote: ... Target sequences in cDNA library were amplified in 10 μl qPCR reaction (5 μl SYBR Green supermix (Bio-Rad #1725121), 0.675 μl 2.5 μM primer mix and 0.45 μl diluted cDNA ...
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bioRxiv - Biophysics 2021Quote: ... folding was quenched by taking 10 μL of sample and adding it to 10 μL of 2x Laemmli SDS gel-loading buffer without reducing agent (BioRad), vortexed and stored at 4°C until analysis ...
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bioRxiv - Biophysics 2021Quote: ... 10 mM borate pH 10 was prepared at 5 μM and loaded onto a 4-20% Mini-Protean TGX pre-cast gel (BioRad). 10 μL of each folding sample was subsequently loaded onto the same gel ...
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bioRxiv - Developmental Biology 2022Quote: Cells in monolayer were fixed in 4% PFA for 20 minutes at 4°C and blocked for 30 minutes in 10% donkey serum (BioRad) and 0,1% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... transferred to PVDF membrane (Amersham Hybond 0.2 um PVDF) at 100 V for 1hr 10 min with mini-protean tetra system (Bio-Rad) at 4 °C ...