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Citations for Bio-Rad :
351 - 400 of 677 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... were combined with Pierce Lane Marker Reducing Sample Buffer (Thermo) and applied onto SDS gels (Mini-PROTEAN TGX Gels 8-16%, BioRad for 24h/48 h/72h samples and a 10% SDS gel for resistance validation) ...
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bioRxiv - Physiology 2024Quote: ... 15-60 μg of denatured and reduced protein was subjected to 8-12% gradient SDS-PAGE followed by transfer to low-fluorescence polyvinylidene difluoride membranes (LF PVDF, BIORAD). Membranes were blocked with LICOR Odyssey TBS Blocking Buffer (LI-COR ...
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bioRxiv - Biochemistry 2024Quote: ... Polyacrylamide gels consisted of a resolving phase of 8-16% (w/v) acrylamide (37.5:1 acrylamide:bisacrylamide, 2.7% crosslinker; Bio-Rad, Watford, UK), 0.375 M Tris-HCl (pH 8.8) ...
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bioRxiv - Bioengineering 2024Quote: ... The target genes were amplified from 8 ng of gDNA template with CFX96 Touch Real-Time PCR (Bio-Rad, USA) under the following conditions ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein quantifications were determined with a Bradford assay then 30μg of protein was separated on a 8-16% or 12% pre-cast gel (Bio-Rad) using primary antibodies diluted 1:500-1:1000 and secondary antibodies (1:2500 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Samples were eluted with 6 mM H2SO4 at a flow rate of 0.6 ml/min from an Aminex HPX 87H organic acid analysis column (300 by 7.8 mm) (BioRad). We recorded the areas for all noticeable peaks associated with detected metabolites (glucose ...
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bioRxiv - Biochemistry 2022Quote: ... in 1% (v/v) acetic acid] for 1 min and photographed using Gel Doc XR system (Bio-Rad).
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bioRxiv - Developmental Biology 2023Quote: ... Isolated RNA was used to synthesize complementary deoxyribonucleic acid (cDNA) with the iScripttm cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... Supernatant was collected and the protein sample concentration was calculated using a bicinchoninic acid (BCA) assay (Bio-Rad).
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bioRxiv - Synthetic Biology 2022Quote: ... MD) equipped with a refractive index detector and a HPX-87H organic acid column (Bio-Rad, Hercules, CA) with the following operating conditions ...
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bioRxiv - Microbiology 2023Quote: ... folic acid 500 μg/L) or on Columbia agar solid support enriched with 5% horse blood (COH) (Biorad). Anoxic conditions were generated in Gaspack (BD ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were stained with a 1:1 mix of glacial acetic acid and Ponceau Red (Bio-Rad) for protein visualisation ...
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bioRxiv - Plant Biology 2021Quote: ... At least 4-6 technical replicate RT-qPCR reactions were performed using iTAQ with ROX and SYBR (BioRad), and 20μL reactions were prepared as per the manufacturer recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.032 % (w/v) SM was applied to a centrifugal device (Micro Bio-Spin 6, Bio-Rad, Hercules) to exchange the buffer to a MS buffer containing 200 mM ammonium acetate (pH 7.4) ...
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bioRxiv - Biophysics 2022Quote: ... and 1 mM EDTA in 99.9% deuterium oxide using a spin desalting column (Micro Bio-Spin 6, BioRad). 15N-HSQC spectra were measured every 40 min for 106 hours at 25 °C on Bruker 600 MHz NMR spectrometer ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was purified by loading quenched samples onto Micro Bio-spin Columns with Bio-Gel P-6 (BioRad), followed by ethanol precipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by 6 washes in 100 µL of PBST in Bio-Spin columns (catalog number 7326204, Bio-Rad). The bound immunocomplexes were eluted via 0.1 M glycine ...
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bioRxiv - Biochemistry 2021Quote: KirBac3.1 protein was buffer-exchanged to 200 mM ammonium acetate (pH=8.0) with 0.5% C8E4 (2×CMC) using a Biospin-6 column (BioRad). The protein sample was loaded into a gold coated needle prepared in-house and analysed on a modified Q-Exactive mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... then washed 6 x 5 minutes with TBS-T before developing with Clarity Western ECL Substrate (Bio-rad).
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bioRxiv - Biochemistry 2023Quote: ... Protein was then cleaned 2-times on Micro Bio-Spin™ P-6 gel Columns (BioRad, #732-6221) where buffer was exchanged with phase separation buffer ...
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bioRxiv - Biochemistry 2022Quote: ... solutions containing purified CytbX were buffer-exchanged into 0.2 M ammonium acetate (pH 8.0) containing 2x CMC of Lauryldimethylamine oxide (LDAO) using Biospin 6 columns (Biorad). 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... then 6 L of RO water with Ca2+ chelating beads (BIO-RAD Chelex 100 Resin Cat#142-1253), and finally 6 L of RO water alone ...
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bioRxiv - Molecular Biology 2024Quote: ... sampels were separated on 6– 15% SDS–PAGE and proteins were transferred to nitrocellulose membrane (1620115, Bio-Rad) using the Trans-Blot Turbo Transfer System from Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Homemade 4% stacking and 6% running gels were run at 60-80V in Tris/Glycine/SDS buffer (BioRad). Transfer was done overnight in 10% methanol in Tris/Glycine buffer (BioRad ...
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The triad interaction of ULK1, ATG13, and FIP200 is required for ULK complex formation and autophagybioRxiv - Cell Biology 2024Quote: ... and 150 mM NaCl utilizing a Bio-Scale Mini Bio-Gel P-6 desalting column (Bio-Rad Laboratories). Subsequently ...
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bioRxiv - Synthetic Biology 2024Quote: ... Images were acquired using a Chemidoc Imaging System and analyzed with Image-Lab v.6 software (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Precision Plus Protein Dual Color Standards Protein Marker was used as a marker (6 μl, #1610374, BIO-RAD).
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bioRxiv - Synthetic Biology 2021Quote: ... The culture supernatant was obtained by centrifugation and used with the Bio-Plex Pro Human Cytokine 8-plex Assay (Bio-rad) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies) and in Criterion cells (Bio-Rad) using respective adapters (LifeTechnologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded on SDS-polyacrylamide gels (8 to 10 %) and transferred to nitrocellulose membranes using Trans-blot turbo transfer system (Bio-Rad). Membranes were saturated for 1 h with TBST buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... containing the crude synaptosomes was resuspended and processed for western blotting in NP-40 lysis buffer as above with the following modifications: equal amounts of protein per sample (unboiled) were separated on 3-8% Tris-Acetate XT gel (Bio-Rad) in XT Tricine running buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... Western blot was performed using 8 or 10% mini gels followed by wet transfer (1 h, 100 V) to Western grade nitrocellulose (Bio-Rad). Blots were incubated in Intercept blocking buffer (LI-COR ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated the supernatant on a 3-kDa cutoff centrifugal filter unit (Amicon) and loaded samples of supernatant on a 8-16% SDS-PAGE TGX Stain-Free gels (Bio-Rad) (SI Figure 1c).
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bioRxiv - Plant Biology 2020Quote: ... then spun down at 12.000g for 15 minutes to remove insoluble material and subsequently analyzed using 12% (in-house gels) or 8-16% SDS-polyacrylamide gels (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... 10 to 30μg of total protein were subjected to 8% or 10% SDS-PAGE gels and transferred electrophoretically onto PVDF membrane by a semi-dry blotting system (Bio-Rad). The membrane was blocked in 5% fat-free milk/Tris-buffered saline for 1 hour and incubated with primary antibodies at 4°C overnight followed by horseradish peroxidase conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 µg of total protein was loaded onto 8 to 15 % SDS-PAGE minigels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Neuroscience 2021Quote: ... were added to the 8 channel droplet generation cartridge according to manufacturer instructions and this cartridge was placed in the QX200 droplet generator (Bio-Rad). Then ...
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bioRxiv - Immunology 2020Quote: ... Samples were heated at 95°C for 3 minutes and run on a 8 – 16% or 4 – 20% TGX-Criterion gel (Bio-Rad). The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... and 2D-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) replicates performed on 11cm 8-16% (w/v) polyacrylamide/Tris-HCl gels (Bio-Rad) using a Criterion Dodeca Cell (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Lysates (20–30 μL) were run on 8–10% polyacrylamide gels and transferred to PVDF membranes (Trans-Blot Turbo Transfer System, Bio-Rad). Membranes were blocked with 5% BSA in TBS (TBS ...
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bioRxiv - Microbiology 2020Quote: ... Flow cytometric staining and analysis was done as described [8] and acquired on ZE5 cell analyzer (Bio-Rad, Hercules, CA, USA). Analysis was performed using Flow Jo software.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips pH 5-8 for CDK4 (BioRad, Hercules, CA, USA) or pH 3–10 for CDK2 (Amersham Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: PCR Products were separated on a 0.8% agarose gel and visualized after staining with DNA Stain Clear G (SERVA, Heidelberg) on a ChemiDoc imaging system (Biorad, Germany).
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bioRxiv - Synthetic Biology 2021Quote: ... 10nM of templates with each promoter type were added to TXTL reactions and incubated at 30 °C for 8 hours (T100 Thermal Cycler, Bio-Rad). Post-incubation ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Native NiNTA lysis buffer (50mM NaH2PO4, 300mM NaCl, pH 8, 10mM imidazole) and protein concentration was measured via Bradford assay (Bio-Rad). 500-800μg of protein were incubated with NiNTA beads at 4°C overnight ...
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bioRxiv - Genetics 2021Quote: ... or 1:8 (for all other quantifications) and 2ul was used to quantify gene expression using iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cell lysates were separated by 8% SDS-PAGE and Western blotted onto a PVDF membrane (semidry transfer, 15 V, 45 min, Trans blot Bio-Rad). The membranes were incubated overnight at 4°C in blocking solution 5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... Thirty micrograms of total protein from each sample were loaded on 8% SDS-PAGE and transferred to PVDF membranes (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Cell lysates were separated on 8-12% SDS-PAGE gel and transferred onto a PVDF membrane (Bio-Rad, Hercules, CA, USA). The membrane was incubated overnight with the respective primary antibodies at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μl of eluted samples were electrophoresed for 8 min at 200 V on a 10% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and protein was stained using Quick Blue Stain ...