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Citations for Bio-Rad :
451 - 500 of 677 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... pH 8.3 at 100 V for 2 h at 2-8 °C using PowerPac Basic setup (Bio-Rad Laboratories Inc., Hercules, CA). Post-electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled at 100 °C for 5 min and loaded in handcrafted 8% SDS-polyacrylamide gel or 4-12% Criterion™ XT Bis-Tris XT Precast Gels (Bio-Rad) and electrophoresed in 1X Tris/Glycine/SDS buffer (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... equal amounts of protein (10-20 μg) were subjected to SDS-polyacrylamide gel (8-15%) electrophoresis and transferred to nitrocellulose (Bio-Rad, #1620115) membranes ...
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bioRxiv - Cell Biology 2024Quote: Cell lysates and membrane fractions were resolved in home-made SDS-PAGE gels ranging from 8% to 18% acrylamide or using 4-20% gradient mini-PROTEAN TGX precast gels (BioRad, Mississauga, ON). Electrophoresis was done in Tris-glycine running buffer (25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were subjected to SDS-PAGE on 8 to 12% of polyacrylamide and samples were run with a PowerPac Basic (Bio-Rad Laboratories) at 90-120 Volts for 60-90 minutes in a Mini-Protean® 3 Cell (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2024Quote: ... Half million of MDCK cells in a cuvette were electroporated with 8 µg plasmids containg the pX459 and the targetting vector with the Gene Pulser Xcell electroporation system (Bio-Rad, USA) at 130 V ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated overnight at 4 °C with rabbit anti-chick IL-6 (Bio-Rad Laboratories, Inc., Hercules, CA) diluted 1:20 in incubation buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Excess dye was removed by two consecutive buffer exchanges through Micro Bio-Spin P-6 desalting columns (Bio-Rad). Protein concentration and labeling efficiency were estimated using a NanoDrop Microvolume Spectrophotometer.
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bioRxiv - Biochemistry 2020Quote: ... 0.14 M NaCl (PBS) followed by removal of the reductant using a Micro Bio-Spin 6 column (Bio-Rad). Columns were washed and pre-equilibrated with 20 mM phosphate buffer pH 7.4 containing 0.1 mM diethylenetriamine-penta-acetic acid (DTPA ...
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bioRxiv - Biochemistry 2021Quote: ... individual proteins were buffer exchanged into 200 mM ammonium acetate pH 8.0 either by SEC or using Biospin-6 (BioRad) columns and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... The annealed doubly nicked DNA duplex was then buffer-exchanged in Hepes 20 mM pH 7.5 with a BioSpin 6 column (BioRad).
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bioRxiv - Biophysics 2022Quote: ... The protein-dye conjugate was purified by 3 rounds of size exclusion chromatography (Bio-Spin 6 column, BioRad, CA) and then aliquoted in 5 μL portions and stored at -80C until required.
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bioRxiv - Microbiology 2022Quote: All proteins were buffer exchanged into 0.2 M ammonium acetate using Bio-Spin P-6 Size Exclu-sion Spin Columns (BioRad) as previously described47 ...
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bioRxiv - Genetics 2022Quote: ... PMOs were buffer exchanged into 25 mM ammonium bicarbonate buffer pH 7.5 using Biospin 6 (Bio-rad, Hercules, California) columns according to the manufacturer’s protocol and then spinning through an 0.22 µm cellulose acetate centrifugal spin filter ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The Chlorella DNA was extracted by isolating the Chlorella and then using a standard 6% Chelex100 resin (Bio-Rad) extraction method ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories). Protein (final concentration 20 µM ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (Healthcare Life Sciences) were loaded into Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... Excess free Texas Red or TAMRA were removed with Bio-spin 6 kDa size exclusion columns (Bio-Rad Laboratories) equilibrated with PBS according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The reactions were run at 125 V for 2.5 hours on a denaturing (6 M urea) 20% (w/v) polyacrylamide gel in 50 mM NaOAc pH 5.2 running buffer (using the Biorad Mini- PROTEAN tetra gel ...
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bioRxiv - Genetics 2023Quote: ... followed by purification and size selection on 1% agarose gel with .6 µg/mL ethidium bromide (BioRad Cat#1610433), selecting for the 7,961 bp band ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 400 µL cold PBS and electroporated with 6 µg of RNA in 0.4-cm electroporation cuvettes (Biorad) with a 950 μF and 260 V pulse using Genepulser system (Biorad) ...
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bioRxiv - Plant Biology 2023Quote: ... Samples containing 6 µg total protein were resolved using pre-cast SDS-PAGE Any KD™ gels (BIO-RAD), transferred to a polyvinylidene difluoride membrane (Immobilon-FL 0.45 µm ...
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bioRxiv - Genomics 2023Quote: ... Excess dye was removed using Micro Bio-Spin® Columns with Bio-Gel® P-6 (cat:7326221, BioRad) by centrifugation at 1000g ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were run at 125 V for 2.75 hours on a denaturing (6 M urea) 20% (w/v) polyacrylamide gel in 50 mM NaOAc pH 5.2 running buffer (using the Biorad Mini-PROTEAN tetra gel ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 x 10^6 cells were electroporated with RNPs in Gene Pulser electroporation buffer (#1652676, Bio-Rad, Hercules, CA) with electroporation enhancer (#1075915 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Imaging was performed using the Silver Stain setting on Image Gel 6 on Gel Doc EZ Imager (Bio-Rad). ImageJ software was used for band intensity quantification as described below.
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bioRxiv - Microbiology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The reaction was stopped with 1N sulfuric acid and absorbance was measured at 450 nm in the iMarkTM Microplate Absorbance Reader (BioRad).
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of total RNA were separated on a 1% agarose gel containing 1X 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and 2.2 M formaldehyde and transferred to a Zeta-probe membrane (Bio-Rad) by capillary transfer in 20X SSC buffer (3 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... and destained with 30% methanol,10% glacial acetic acid in double distilled water and subjected to film by ChemiDoc systerm (BioRad).
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bioRxiv - Physiology 2020Quote: ... Samples and standards were incubated with bicinchonic acid for 30 minutes prior to measuring absorbance as 540 nm using an iMark microplate absorbance reader (BioRad).
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bioRxiv - Biochemistry 2022Quote: ... Gels were Coomassie stained and destained with 10% acetic acid prior to imaging on a ChemiDoc MP imaging system (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was measured using a bicinchoninic acid (BCA) protein assay (Pierce) or Bradford assay (Bio-Rad, Cat. No. 5000006). Proteins were prepared in 1× Laemmli buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were analyzed on a 4-15% polyacrylamide NuPage Bis-Tris gel (invitrogen) in 1x morpholineethanesulfonic acid (MES) SDS running buffer (invitrogen) or on a 4-20% polyacrylamide Criterion gels (BioRad) in 1x Tris/glycine/SDS buffer (BioRad ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... The gel was then destained for 15 minutes in 7.5% (v/v) acetic acid before being imaged on a Chemidoc imaging system (Bio-Rad). For western blots ...
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bioRxiv - Microbiology 2022Quote: ... A Bio-Rad Aminex HPX-87H HPLC column for organic acid analysis (300 × 7.8 mm; Bio-Rad Hercules, CA, USA) was equilibrated with 5 mM H2SO4 (Titrisol ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was resolved by electrophoresis on a 6% non-denaturing polyacrylamide gel in TBE (100 mM Tris, 90 mM boric acid, 1 mM EDTA) buffer and visualised by ChemiDoc (BioRad) using SYBR Gold stain or imaging in the 6FAM ...
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bioRxiv - Developmental Biology 2024Quote: ... The reacted proteins were detected by incubating the membranes in the chemiluminescence solution (luminol, coumaric acid, hydrogen peroxide, Tris pH 6.8) and imaged by the ChemiDoc Gel Imaging System (BioRad, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... which binds non-selectively to nucleic acids and was imaged by the ChemiDoc Imaging System from Bio-Rad (California, USA). The bands were subsequently quantified using ImageJ software ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Protein separation was done by 1D SDS-PAGE electrophoresis using commercial gels to ensure reproducibility (8–16% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad). A sample of venom of ISm and ISy lines ...
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bioRxiv - Neuroscience 2020Quote: ... Diabetes-associated markers and cytokine levels in the plasma were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (Cat# 171F7001M; Bio-Rad, Carlsbad, California) and the Bio-Plex Pro Mouse Cytokine 23-Plex Assay (Cat# M60009RDPD ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein (5–20 μg) was resolved onto 8–12% SDS-PAGE and blotted onto 0.2 μm pore size PVDF membranes (1620177, Bio-Rad, Hercules, CA, USA). Primary antibodies were incubated overnight at 4°C after blocking membranes for 1h ...
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bioRxiv - Microbiology 2021Quote: All melt-MAMA PCRs were performed on a CFX96 Real-Time PCR Detection System using white hard shell 96-well PCR plates or 0.2 mL 8-tube PCR strips (Bio-Rad, Gladesville, NSW, Australia), and PCR results examined using the CFX Maestro v4.1.2433.1219 software (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg of ΔORF3-E mNG RNA and 20 μg of N-gene RNA were electroporated into 8×106 Vero-ORF3-E cells using the Gene Pulser XCell electroporation system (Bio-Rad, Hercules, CA) at a setting of 270V and 950 μF with a single pulse ...
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bioRxiv - Immunology 2020Quote: ... 30-50µg of protein per lane alongside a prestained molecular weight protein marker was separated on an 8% SDS PAGE gel prepared from SDS-PAGE kit (Beyotime, China) and electrotransferred to immunoblot PVDF membrane (BIO-RAD, CA, USA) for protein blotting ...
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bioRxiv - Microbiology 2023Quote: Serum samples collected from mice were tested in duplicate in a multiplex immunoassay using magnetic beads (Mouse Th1-Th2 8-plex Bio-Rad, Hercules, CA) using the Luminex MAGPIX® system) ...
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bioRxiv - Cancer Biology 2024Quote: ... Serum hormones were quantified on a Luminex MAGPIX platform using the Bio-Plex Pro Mouse Diabetes 8-Plex and Adiponectin Assays (Bio-Rad, Hercules, CA) and the IGF-1 mouse Luminex Discovery Assay (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... Equal amount of total protein (25 μg/well) was resolved by electrophoresis on 8% Tris-Acrylamide gels and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad Laboratories, CA, US). Membranes were blocked for 1 h at room temperature in 5% skim milk diluted in Tris-buffered saline supplemented with 0.1% Tween 20 (TBST) ...