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Citations for Bio-Rad :
501 - 550 of 677 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Serum samples collected from mice were tested in duplicate in a multiplex immunoassay using magnetic beads (Mouse Th1-Th2 8-plex Bio-Rad, Hercules, CA) using the Luminex MAGPIX® system) ...
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bioRxiv - Biochemistry 2023Quote: ... plates were stained with primuline (0.05% in acetone/water, 8/2; v/v) and photographed under long-wave UV light (Biorad ChemiDoc XRS Imaging System).
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bioRxiv - Microbiology 2023Quote: ... for the selected clinical isolates using the indicated concentrations of antibiotics via an alamar blue reduction assay completed as described but without shaking.8 After 3 days of incubation with the alamar blue reagent (BioRad, Hercules, CA, USA), the MIC was identified as the lowest concentration of drug that inhibited the reagent color change as determined visually.
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Both fractions were analyzed on SDS-PAGE and western blot using 8–16% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). For immunoblotting of Rubisco ...
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bioRxiv - Biophysics 2021Quote: ... The uncoupled BG-GLA-NHS was removed by filtration on Micro Bio-Spin™ P-6 Gel Columns (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... approximately 250.000 cells were plated in a 6-well dish and transfected with a final concentration of 22.5 nM siRNA using siLentFect Lipid Reagent (Bio-Rad) accordingly to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... MtCoaBC and MsCoaBC were exchanged into NH4OAc (500 mM, pH 7.0) solution using Micro Bio-Spin 6 chromatography columns (Bio-Rad). A sample volume of 2.5 μL was injected into a borosilicate emitter (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: DNA isolated from cells by phenol:chloroform isolation and ethanol precipitation was cleaned on Micro Bio-Spin P-6 SSC columns (Bio-Rad) according to manufacturer protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Captured elution fraction from the Strep-Tactin-affinity purification were loaded onto a anion exchange column (UNO Q-6, Biorad) with a flowrate of 4 ml/min ...
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bioRxiv - Biochemistry 2021Quote: ... pH 5.3 (murine P-domains) or pH 7.3 (GII.4 Saga P-domain) at 4 °C via Micro Bio-Spin 6 columns (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and CyaY were buffer exchanged into 200 mM ammonium acetate (pH = 7.5) using Micro Bio-Spin 6 Columns (Bio-Rad). Instrument parameters were tuned to maximize transmission but simultaneously preserve the native-like state of proteins as determined by ion mobility ...
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bioRxiv - Neuroscience 2020Quote: ... IL-6) were quantified using a real-time PCR detection system and SsoAdvanced Universal SYBR Green Supermix kit (BIO-RAD). In brief ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing protein were pooled and desalted to remove imidazole by gel filtration on a 50 mL BioScale P-6 column (BioRad) using resuspension buffer as the eluant.
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bioRxiv - Biochemistry 2020Quote: ... the cleaved His6 tag was removed and the enzymes were buffer exchanged into 200 mM ammonium acetate (pH 7.5) using Micro Bio-Spin 6 columns (Bio-Rad). His6-affinity tagged HalA2 was likewise digested with thrombin to remove the His6 tag ...
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bioRxiv - Plant Biology 2021Quote: ... The IEF was performed on the 7cm length gel strips with immobilized pH gradients 3-10 and 3-6 (BioRad). After IEF ...
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bioRxiv - Biochemistry 2022Quote: ... the enzymes were buffer exchanged into their respective reaction buffers above using a Micro Bio-Spin 6 size exclusion spin column (Biorad) to remove glycerol and other ion suppressing constituents ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to PVDF membrane at 4°C for 14.5 h at 30 V using the mini trans-blot module in transblot buffer (10 mM MES buffer pH 6 and 10% (v/v) methanol) according to the supplier’s instructions (Bio-Rad). The membrane was removed from the cassette ...
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bioRxiv - Biophysics 2021Quote: ... and 2 times the critical micelle concentration (CMC) of various detergents (namely LDAO and C8E4) using a Biospin-6 column (BioRad) and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
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bioRxiv - Cell Biology 2020Quote: ... gossypii proteins) or TBS (S. cerevisiae proteins) supplemented with 1 mM DTT using pre-equilibrated BioSpin-6 spin columns (BioRad) or ...
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bioRxiv - Systems Biology 2021Quote: ... and 6 μl for slow growing mutants with lower OD600) on Stain-Free gels (4-20%, CAT # 4568096, Bio-Rad, Tris/Glycine SDS Buffer (CAT # 161-0732 ...
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bioRxiv - Microbiology 2021Quote: ... for 60 min at 37°C followed by purification on a Micro-Bio Spin P-6 Gel Column (Bio-Rad). 1 pmol of 5’-end-radiolabeled primer was used for each extension reaction ...
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bioRxiv - Biochemistry 2021Quote: APRT2-Ntag and APRT2-Ctag were buffer exchanged into a volatile buffer (200 mM ammonium acetate, pH 7.4) using a Micro Bio-Spin 6 device (Bio-Rad). Samples were loaded into pulled glass capillaries prepared in house ...
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bioRxiv - Immunology 2021Quote: ... before buffer exchange into 500 mM ammonium acetate solution (pH 7.5) using a Micro Bio-Spin 6 column (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... were exchanged from size exclusion buffer into 2 M ammonium acetate pH 7.4 via a single-pass through a Bio-Spin P-6 column (Bio-Rad). Buffer-exchanged protein was loaded into gold-coated glass capillaries (1.0 mm OD/0.75 mm inner diameter with filament) ...
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bioRxiv - Molecular Biology 2024Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE buffer (pH 8.0) and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
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bioRxiv - Plant Biology 2023Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE (pH 8.0) buffer and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
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bioRxiv - Biochemistry 2023Quote: ... The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad). Molecular weight of standard was as follows ...
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bioRxiv - Biochemistry 2023Quote: ... CsgA and CsgAslowgo samples were buffer-exchanged into MS-compatible buffer (150 mM Ethylene diamine diacetate pH 6.3, 10mM TMAO) using Micro Biospin 6 desalting columns (Bio-Rad) to a final protein concentration of 3-5 μM ...
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bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
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bioRxiv - Cancer Biology 2024Quote: As previously described,(6) we used the Bio-Plex Pro Human Chemokine 40-plex Panel (Bio-Rad Laboratories, Hercules, California) on a Luminex 200 System to measure plasma cytokines ...
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bioRxiv - Biochemistry 2024Quote: ... The gels were run at 6 W/gel and followed by Cy3 visualization using the Molecular Imager PharosFX system (BioRad).
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bioRxiv - Biochemistry 2024Quote: Purified membrane proteins were buffer exchanged into 200 mM ammonium acetate (pH 7.4 at room temperature adjusted with ammonium hydroxide) supplemented with 2x CMC of the desired detergent using a centrifugal desalting column (Micro Bio-Spin 6 columns, BioRad). For the AmtB-GlnK complex ...
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bioRxiv - Biochemistry 2024Quote: AcrAS was buffer exchanged into a volatile solution (100 mM ammonium acetate, pH 6.0) using a centrifugal exchange device (Micro Bio-Spin 6, Bio-Rad) according to manufacturer’s instructions native MS experiments were performed on a Synapt G2-Si mass spectrometer (Waters) ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 mM ammonium acetate at the desired pH using a centrifugal buffer exchange device (Micro Bio-Spin 6, Bio-Rad). Stock solutions of lipids were prepared from chloroform/methanol solution by evaporating aliquots of known volume in a SpeedVac ...
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bioRxiv - Microbiology 2024Quote: ... and 0.25µM of each 5’ 6-FAM/ZEN/3’ IBFQ or 5’ HEX/ZEN/3’ IBFQ probe with the QX200 Droplet Digital PCR System (Biorad). Reactions were performed using the following PCR cycle settings ...
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bioRxiv - Biochemistry 2024Quote: ... Purified GroEL and GroES were first buffer-exchanged into RNC low-salt buffer using Micro Bio-Spin 6 Columns (Biorad) to remove any traces of Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were generated by sonicating and boiling 1*10^6 cells in 100ul of 2X Laemmli Buffer (Bio-Rad) with BME ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction products were resolved in a 15% UREA-PAGE gel and the 6-FAM signal from the RNA visualized in a ChemiDoc gel imager (BioRad).
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bioRxiv - Genetics 2024Quote: ... was added to the reactions and the products were separated on 6% polyacrylamide gels (ratio acrylamide:bisacrylamide 19:1, Bio-Rad) in TAE (40mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... The reaction was stopped using glacial acetic acid and the N-glycans desalted sequentially using strong cation exchange (AG 50W X8, Bio-Rad), C18 and porous graphitised carbon (PGC ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Incorporation of radioactive amino acids was determined by liquid scintillation counting and normalized to the cellular protein content (quantified with DC Protein Assay (Bio-Rad)).
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bioRxiv - Microbiology 2022Quote: ... Reaction was developed using a freshly prepared TMB substrate solution and stopped with 1M Sulfuric acid before determining the OD450 using a microtiter plate reader (iMark mic, Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... methanol in 1X TBE buffer with 10% (v/v) acetic acid) and RNA was visualised using the Silver Stain Plus Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... 12 % SDS-PA gels were fixed with fixation solution (40 % ethanol, 10 % acetic acid) o/n and stained in Flamingo fluorescent protein dye (Bio-Rad) for up to 6 h and imaged with the ChemiDoc (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were then stained with Coomassie Blue solution, followed by de-staining (Methanol, Acetic Acid solution) and visualised by using Gel Doc™ XR+ system (BioRad).
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bioRxiv - Biochemistry 2023Quote: Intracellular and soluble-secreted proteins were Deoxycholate/Trichloroacetic acid– precipitated and separated by SDS-PAGE on gradient 4-20% PROTEAN TGX Stain-Free Gels (Bio-rad) and proteins were transferred to polyvinylidene difluoride membranes within Trans-Blot Turbo Transfer Packs (Bio-rad ...
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bioRxiv - Cell Biology 2023Quote: ... for protein extracted from cultured cells or brain sections and micro bicinchoninic acid (microBCA) for protein extracted from EVs according to the manufacturer’s instructions (Bio-Rad Laboratories).
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bioRxiv - Biophysics 2022Quote: ... Gels were fixed for 10min (50% methanol, 10% acetic acid) and stained with Coomassie Blue for imaging on a gel doc (Bio-Rad). Band intensities were quantified using Image Lab (Bio-Rad ...