Labshake search
Citations for Bio-Rad :
3801 - 3850 of 4995 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2024Quote: ... at 4°C in a cold room using transfer stacks (from Trans-Blot Turbo RTA kit, Bio-Rad, 1704272). Next ...
-
bioRxiv - Bioengineering 2024Quote: ... The eluted proteins were resolved on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4561094) and transferred onto 0.45 µm PVDF membranes (Bio-Rad ...
-
bioRxiv - Cancer Biology 2024Quote: An equal amount of total protein was resolved on precast 4%–12% SDS-PAGE gels (Biorad, Hercules, CA, USA), and the protein was transferred onto PVDF membranes as previously described 2 ...
-
bioRxiv - Cell Biology 2024Quote: ... onto gradient Bio-Rad 4-20% polyacrylamide SDS-PAGE gels containing StainFree total protein stain (Bio-Rad, 456-8095) and run at 125V in SDS-PAGE running buffer (250.1 mM Tris ...
-
bioRxiv - Cell Biology 2024Quote: ... 15ug of protein were loaded and ran in 4–20% Mini-Protean TGX precast protein gels (Bio-Rad, 4561094) in Tris-Gly-SDS buffer ...
-
bioRxiv - Cancer Biology 2024Quote: ... 50 mg lysate as input for each condition were loaded onto 4%–15% TGX gels (Bio-Rad, Hercules, CA). Proteins then were transferred onto nitrocellulose membrane and blotted with antibodies against proteins of interest ...
-
bioRxiv - Cancer Biology 2024Quote: ... Each fraction of the C-DGUC purified sEVs (37.5 μL) was mixed with 12.5 μL of 4 × Laemmli Sample Buffer (Bio-Rad) and 10% 2-Mercaptoethanol (Bio-Rad) ...
-
bioRxiv - Cell Biology 2024Quote: ... Migration of proteins lysate was performed in 4-12 % gradient or in 10 % SDS-PAGE gels (Bio-Rad Laboratories), transferred onto PVDF membranes (Millipore) ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were boiled before being subjected to SDS-PAGE on Mini-PROTEAN TGX Precast Gel 4-15 % (Biorad, 4561086). Western blot using anti-SUMO antibody (non-commercial ...
-
bioRxiv - Cell Biology 2024Quote: ... 20µg protein of each processed sample was loaded per well on 4-20% Criterion TGX SDS-PAGE gels (Biorad) for electrophoresis ...
-
bioRxiv - Developmental Biology 2020Quote: ... Membranes were then washed 3 times for 5 min in TBST before detection using Clarity Western ECL Substrate (Bio-rad, 170-5061) and imaging on a ChemiDoc MP (Bio-rad ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1hr on ice with varying concentrations of fluorescently labeled hybridoma-derived or recombinant mAbs and then washed x3 in flow buffer (PBS + 3% fetal calf serum) prior to analysis on a ZE5 flow cytometer (BioRad, Hercules, CA). Equilibrium binding affinities were calculated via nonlinear regression (One site – total and non-specific binding ...
-
bioRxiv - Molecular Biology 2020Quote: ... carried out in glass tubes filled with polyacrylamide gel using a mixture of ampholytes 3/10 and 5/8 (Bio-Rad, USA) overnight at 4 °C ...
-
bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
-
bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...
-
bioRxiv - Bioengineering 2022Quote: ... 6 ng of cDNA was used per qPCR reaction consisting of gene-specific primers (Supplementary Information Table 3) and amplified with the PowerSYBR Green Master Mix (Applied Biosystems in a CFX-96 thermocycler (Bio-Rad). Data was acquired using the Bio-Rad CFX Manager 3.1 ...
-
bioRxiv - Bioengineering 2022Quote: ... The PVDF membrane was then washed 3 × 10 min with TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse (diluted 1:20,000; Bio-Rad, Hercules, CA) secondary antibody for one hour at room temperature followed by 6 × 10 min washes with TBST and detection using the Radiance Plus chemiluminescence substrate (Azure Biosystems ...
-
bioRxiv - Molecular Biology 2021Quote: ... Band intensities of at least 3 individual experiments were measured densitometrically and normalized to whole protein using the Image Lab Software 5.1 from BioRad (Biorad, Munich, Germany).
-
bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
-
bioRxiv - Neuroscience 2022Quote: ... All reactions were performed using primers listed in the supplementary table 3 and the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). Relative expression levels were determined according to the ΔΔCt method with the human housekeeping gene ABELSON as endogenous control for normalization as previously described (Beillard et al. ...
-
bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
-
bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using 3 ng (neutrophils) and 10 ng (monocytes) of total RNA and iScript™ Reverse Transcription Supermix (Bio-Rad). Relative quantitation of mRNA expression levels was determined with CFX96 Touch™ Real Time PCR using SsoAdvanced™ Universal SYBR Green Supermix and PrimePCR™ Primers (Bio-Rad) ...
-
bioRxiv - Genomics 2020Quote: ... (see Supplementary Table 3 for a full list of oligos used in the present study) into 384-well hard-shell PCR plates (Bio-Rad HSP3901) using Mantis liquid handler (Formulatrix) ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
-
bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
-
bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated using a 12% SDS-PAGE and mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
-
bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
-
bioRxiv - Systems Biology 2023Quote: ... was applied for 3 min before the membranes were imaged using the ChemiDocXRS+imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Quantification of proteins was conducted using Image J software (Version 1.52a ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
-
bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories). Horseradish peroxidase (HRP)-conjugated antibodies against β-actin (Cell Signaling ...
-
bioRxiv - Biochemistry 2023Quote: ... The membranes were washed three times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories).
-
bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
-
bioRxiv - Developmental Biology 2024Quote: ... and electroporated (square wave, 6 pulses, 30 V, 3 ms duration, 100 ms interval) using a gene Pulser XCell electroporator (Bio-Rad, 1652660). Zygotes were recovered and transferred to fresh KSOM drops and cultured overnight ...
-
bioRxiv - Immunology 2024Quote: ... The membranes were washed five times for 5 min each with TBST and incubated with 1 mL of chemiluminescent detection reagent (Cytiva, RPN2232) for 3 min before image acquisition using a ChemiDoc MP Imaging System (Bio-Rad Laboratories). Horseradish peroxidase (HRP)-conjugated antibodies against β-actin (Cell Signaling ...
-
bioRxiv - Plant Biology 2024Quote: ... The protein extracts were resolved by 12% (w/v) SDS-PAGE using a Bio-Rad Mini PROTEAN 3™ electrophoresis unit (Bio-Rad) and electrotransferred onto Hybond ECL nitrocellulose sheets (GE Healthcare ...
-
bioRxiv - Biochemistry 2024Quote: ... The primer sequences for detecting ACTB are 5’-GACCTGACTGACTACCTCAT-3’(forward) and 5’-TCTCCTTAATGTCACGCACG-3’ (reverse) Quantitative PCR (qPCR) reactions were conducted using the SYBR green PCR master mix (Bio-Rad, 1725121) in CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Cell Biology 2024Quote: ... Gel was transferred at 350 Amperes for 120 minutes onto pre-activated polyvinylidene difluoride (PVDF) or Nitrocellulose membranes in a Mini-Protean® 3 Cell (BioRad Laboratories). Correct protein transfer was checked by red Ponceau S Solution (Panreac) ...
-
bioRxiv - Cell Biology 2024Quote: ... and then transferred onto a polyvinylidene fluoride (PVDF) membrane at constant voltage of 25V for 3 min using Trans-Blot TurboTransfer Packs (Cat.: 1704156, Lot.: 64340580, Bio-Rad, USA) and Trans-Blot Turbo system (Cat. ...
-
bioRxiv - Microbiology 2020Quote: ... Two microgram of concentrated viruses were resolved on 4-20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gels (Biorad) using the Novex™ Sharp Pre-stained Protein Standard (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were cooled briefly and loaded onto 4-20% pre-cast gels (Bio-Rad Mini-PROTEAN TGX Gels Cat# 4561095) and run at 100-120V using the Bio-Rad Mini-PROTEAN Tetra System ...
-
bioRxiv - Systems Biology 2020Quote: ... Immunoblotting was performed using SDS-PAGE with 12.5% acrylamide or 4-15% gradient gels (Bio-Rad, Cat #4561046, Cat #4561086).
-
bioRxiv - Cell Biology 2020Quote: Samples were resolved on Mini-Protean® TGX 4–20% gels or TGX 7.5% gels (BioRad Laboratories, 4568095 and 4568025). Separated proteins were transferred to Trans-Blot Turbo 0.2-μm PVDF membranes (BioRad ...
-
bioRxiv - Cell Biology 2020Quote: ... In short: OCT is removed with PBS and then tissue is placed in a cold 4% polyacrylamide (1610140 Bio-Rad) hydrogel with 0.25% photoinitiator (VA-044 ...
-
bioRxiv - Cell Biology 2020Quote: ... Lyophilized proteins were dissolved in SDS-PAGE sample buffer and resolved by SDS-PAGE (BioRad precast 4-15 % gradient gel). The gel was stained with Simple Blue Safe Stain (Invitrogen) ...
-
bioRxiv - Cell Biology 2020Quote: ... The protein extracts were resolved on 4-15% Criterion™ Tris-HCl precast gels (#3450029, Bio-Rad Laboratories, CA, USA) and blotted onto PVDF membranes (#1704157 ...
-
bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were prepared for mass spectrometry by disrupting total or immunopurified ribosomes in 1X Laemmli buffer and running on a 4-20% Mini-PROTEAN TGX Precast Protein Gel in 1X Tris/Glycine/SDS buffer (BioRad) for 5 minutes ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.1 OD equivalents of the resulting sample was subjected to SDS-PAGE and the proteins were separated on 4-15% Mini-PROTEAN-TGX strain-free gels (BioRad). For subsequent immuno-blotting ...
-
bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...