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Citations for Bio-Rad :
3801 - 3850 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... A 250 μL suspension of cells and 500 ng of double-digested (XbaI and KpnI) vector plasmid were incubated for 5–10 min on ice in a 4-mm Gene Pulser®/MicroPulser™ cuvette (BioRad, Hercules, CA). GenePulser XCellTM (BioRad ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were then subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad Mini-PROTEAN® TGX™ 4-15% Gel) according to manufacturer’s protocols for electrophoresis at 110 V for 70 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 to 30 μg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Immunology 2021Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-Rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Bead pellets were resuspended and boiled in β-mercaptoethanol containing Laemmli sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts (50 µg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cell Biology 2022Quote: ... The radio-labelled proteins were then separated with SDS-PAGE on a 4-12% Criterion XT Bis-Tris gels (Bio-Rad, Hercules, CA, USA) in MES buffer (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The proteins in the lysates were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis using gradient (4–20 %) PAGE gels (Bio-Rad, Mini-PROTEAN TGX), with a molecular mass marker (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... the indicated ESA supernatant was boiled in SDS-PAGE sample buffer and 3 μg of each sample was run with a 4-15% TGX gradient gel (Bio-Rad cat. no. 4561086), then transferred to a nitrocellulose membrane ...
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bioRxiv - Genetics 2022Quote: ... 30μg protein for iPSC-derived optic cups or 15μg for iPSC-LESCs were loaded onto 4-15% Mini-PROTEAN ® TGX ™ gels (BioRad Inc., CA, USA) and transferred to an Immun-Blot™ PVDF membrane using a Trans-Blot® SD semi-dry transfer cell (BioRad Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded onto 4-12% Criterion XT precast gels and proteins were separated by SDS-PAGE under reducing conditions (Bio-Rad Laboratories, Hercules, CA). The proteins were then electroblotted onto a 0.45 μm nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... by tank blotting at 250mA for 90 min in a cold room (4 °C) in blotting buffer (catalog#1610734, Bio-Rad, Hercules, CA, USA). The membrane was blocked for 2 h in TBS-T (tris buffered saline supplemented with 0.1 % Tween ...
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bioRxiv - Immunology 2022Quote: The purity and quality of the proteins were characterized at all stages of the purification process using 4%-20% SDS–PAGE (Bio-Rad, Catalog number 4561096) and 3%-12% Bis-Tris gel (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins (40 μg/lane) were resolved on a 4–20% Mini-PROTEAN® TGX Stain-Free™ precast polyacrylamide gel (Cat.#4568094; Bio-Rad Laboratories) and transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: Approximately 15 μg of synaptosome protein was separated on a precast gradient gel (4-15% Mini-PROTEAN® TGX™ Precast Protein Gels, 4561086, BioRad, United Kingdom) and transferred to nitrocellulose membrane (Amersham™ ProtranÒ Western Blotting Membrane ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples containing 30 μg of total protein were separated by SDS-PAGE for 30 min at 270 V using 4–20% gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2024Quote: ... 10 μg of total protein for both NT and Rab siRNA-transfected samples were resolved in 4-20% gradient SDS-PAGE (Mini-PROTEAN Gel, Cat. 4561094, Bio-Rad Laboratories, Hercules, CA) and subsequently transferred to a PVDF membrane for immunoblotting (Immun-Blot PVDF Membrane ...
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bioRxiv - Microbiology 2023Quote: ... Samples were boiled in Laemmli sample buffer before separation by SDS-PAGE on 4-20% TGX™ precast gels (Bio-Rad, Hercules, CA, USA). Gels were transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Developmental Biology 2024Quote: Protein lysates (40 µg per lane) from uteri or oviducts were loaded on a 4-15% SDS-PAGE gel (Bio-Rad, Hercules, CA, USA), separated in 1X Tris-Glycine Buffer (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–20% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a PVDF membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Developmental Biology 2023Quote: Samples were run on 10% or 4-15% TGX pre-cast gels with 10 wells with 50 μL well volume (Bio-Rad, #4561034 and #4561084). Proteins were transferred overnight onto PVDF membrane (wetted briefly with methanol ...
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bioRxiv - Biophysics 2023Quote: ... the protein concentrations were determined by image analysis of SDS-PAGE with 4-20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (Bio-Rad, 4568096) followed by Coomassie Brilliant Blue staining using the following concentration standard ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 50-60 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 20 μg of protein extracts were loaded per well in 4-15% Mini-PROTEAN® TGXTM Precast Protein gels (Bio-Rad cat# 4561084). After electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... and denaturated by β-mercaptoethanol at 75°C before loading 20-30 µg protein onto 4-20% Mini-PROTEAN TGX Stain-FreeTM Protein Gels (Bio-Rad, Hercules, CA, USA) and electrophoresed at 120 V for 120 min ...
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bioRxiv - Physiology 2023Quote: ... 5 μg of protein homogenate was separated on Criterion™ XT Bis-Tris (RyR, SERCA, Actin) or 4–15% Mini-PROTEAN® TGX™ Precast stain-free (CaMKIIδ) gels (Bio-rad) and transferred at 4°C for 16 hrs at 30 V onto 0.45 µm supported nitrocellulose membrane (Bio-rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 to 30 µg of lysate protein was subjected to SDS-PAGE electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... 40ug of each sample was denatured at 95°C for 5 minutes and loaded in 4–20% Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad; SA) with a gel lane including a Dual Xtra standard protein ladder (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... 25ug of protein was used for Western blot run in 4-20% gradient gel (Criterion TGX Stain-free, #5678094, Bio-Rad, Hercules, California, USA). Proteins were transferred to nitrocellulose membrane in Turboblotter (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting proteins were separated by polyacrylamide gel electrophoresis (4%–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, Bio-Rad, California, USA) and then transferred to a nitrocellulose membrane (Reference ...
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bioRxiv - Genetics 2023Quote: ... The purity of viral preparations was determined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis on a 4– 15% Mini-PROTEAN TGX Stain-Free Gel (Bio-Rad, Hercules, CA, USA) in Tris-glycine- SDS buffer (Figure S3).
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bioRxiv - Bioengineering 2023Quote: ... was subjected to SDS-PAGE using a 4-20% Mini-PROTEAN TGX acrylamide gel on a Mini-PROTEAN Electrophoresis cell (Bio-Rad Laboratories, CA, USA). Electrophoretically separated proteins were transferred to Immobilon-P PVDF membrane (25V/RT/overnight) ...
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bioRxiv - Neuroscience 2024Quote: ... 30µg of protein homogenates were separated by SDS-PAGE on a 4-12% gradient Bis-Tris gel (Bio-Rad, Hercules, CA, Criterion XT 3450125) in XT MES buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (10 µg of protein per well) were separated on SDS-polyacrylamide gels (CriterionTM TGXTM Precast Gels, 4-15%; Bio-Rad, Hercules, CA, USA) using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently subjected to SDS-polyacrylamide gel electrophoresis (PAGE) on a 4–15% Mini Protean TGX precast gel (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Animal Behavior and Cognition 2024Quote: Whole cell lysates containing SDS buffer were homogenized with a 27-G syringe and resolved on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, 4568096) or 6/10/12% home-made polyacrylamide gels and transferred to nitrocellulose membranes (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... A 40 µg of protein of CFS samples and 10 ng of purified rGdhA were resolved in a 4-20% SDS-PAGE (Bio-Rad, Hercules, CA; # 4561094) and immunoblotted with affinity purified anti-rabbit rGdhA antibody (0.3 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... at 95°C for 10 mins before being loaded into 4-20% Mini-PROTEAN® TGXTM Precast Protein Gels (15 wells, 15uL; Bio-Rad, CA, USA) and subject to electrophoresis on a Bio-Rad Mini-PROTEAN® Tetra Cell system (180 V ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µg of each lysate sample were separated though sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4-20% Criterion precast gels (Bio-Rad Cat #456-1095). Proteins were blotted onto Polyvinylidene difluoride (PVDF ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216, Anti-FLAG; 1:2000 dilution, Sigma-Aldrich #F1804, anti-β-Tubulin; 1:1000 dilution, Bio-Rad #12004166). Then membranes were washed with 1X TBST 3 times (10mins each wash ...
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bioRxiv - Plant Biology 2023Quote: ... followed by addition of primary antibodies at 4 °C overnight with rotation (mouse anti-ϕ3-actin [Proteintech, 66009-1-Ig], 1:1000, and rat anti-χξ-tubulin [YOL1/34, MCA78G, Bio-Rad], 1:1000). The specimen was washed three times with PBST and incubated with secondary antibodies (anti-mouse ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were anti-mouse (Bio-Rad, #170-6516, dilution 1:1000 or 1:2000) and anti-rabbit (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... 1:5000 and 1:10000 dilutions of the libraries in triplicate on iQ5 (Bio-Rad) and CFX96 Touch qPCR machines ...
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bioRxiv - Plant Biology 2021Quote: ... 0.05% SDS) and a semi dry transfer cell (Bio-Rad, 1 mA/cm2, 1 h). Membrane was blocked with protein free blocking solution T20 (Pierce ...
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bioRxiv - Microbiology 2019Quote: ... was diluted 1:5000 in 1% (w/v) casein blocking buffer (Bio-Rad, 161-0782). Blots were incubated at room temperature for 2 h ...
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bioRxiv - Immunology 2020Quote: ... A total of 50μg protein was diluted 1:1 with 2x Laemmli buffer (Bio-Rad), resolved by SDS-PAGE and transferred into PVDF membranes ...
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bioRxiv - Bioengineering 2021Quote: ... samples were mixed 1:1 with 2x Laemmli sample buffer with 2-mercaptoethanol (Bio-Rad) and boiled for 10min ...
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bioRxiv - Biochemistry 2021Quote: Purified protein was mixed in 1:1 ratio with 2X laemmli loading dye (Bio-rad) prepared according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1× iTaq Universal SYBR Green Supermix or 1× iTaq Universal Probe Supermix (Bio-Rad, USA), 300 nM each of forward primer (late RT product ...
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bioRxiv - Physiology 2021Quote: ... Sections were incubated with F4/80 antibody (Clone Cl:A3-1, Bio-Rad, MCA497R, 1:100) followed by biotinylated antibody (Vector ...