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Citations for Bio-Rad :
3651 - 3700 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... The solution with MSP1E3D1 was mixed at 4°C for 10 min before addition of 130 mg of Biobeads SM2 (Bio-Rad, Hercules, CA). Biobeads (washed into methanol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A total of 100 μg brain lysate was run on a Bio-Rad Mini-Protean TGX 4-20% polyacrylamide gel (4561094, Bio-Rad, Hercules, CA) at 200V for approximately 40 minutes ...
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bioRxiv - Microbiology 2020Quote: Protein digestion was confirmed by running treated and untreated culture filtrate on precast polyacrylamide gel 4-20 % (Mini-PROTEAN® TGX Stain-Free™ Precast Gels, BIO-RAD) at 200V for 40 mins ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant containing approximately 15-30 μg protein was separated by SDS-PAGE using a 4-20% gradient Mini-PROTEAN TGX Precast Gel (Bio-Rad, Hercules, CA) and then transferred to a 0.2 μM nitrocellulose membrane ...
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bioRxiv - Biochemistry 2020Quote: Surface biotinylation samples were heated to 50°C for 10 minutes before separation using 4-20% polyacrylamide gradient gels (Bio-Rad, Hercules, CA). After separation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were heated at 95°C for 5 min prior to loading onto 4-20% Mini-PROTEAN® TGX™precast polyacrylamide gel (Bio-Rad). Gel electrophoresis was done at 100 V for 90 min ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were quantified by the Bradford method and 20 to 30 μg of total protein extract were loaded on 4-20% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad). After transfer onto nitrocellulose membrane ...
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bioRxiv - Immunology 2019Quote: Intestinal epithelial cells lysate (20 µg per lane) were separated by SDS-PAGE through 4%-20% Mini-PROTEAN® TGX™gel (BIO-RAD), transferred to nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2019Quote: ... and equal amounts of total protein (10 to 20 µg) were loaded on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad). All samples for a participant were loaded in adjacent lanes on the same gel ...
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bioRxiv - Neuroscience 2021Quote: Denatured total cellular protein samples were separated on SDS polyacrylamide gels 4-15% Mini-PROTEAN® TGX Stain-Free™ gels (Bio-Rad) and transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... samples of 5-10 μg protein were reduced with 50 mM dithiothreitol (DTT) for 15 minutes at 90°C and mixed with 4× sample loading buffer (Bio-Rad, Munich, Germany). Protein separation was carried out via SDS-PAGE in 12% polyacrylamide gels (Mini-PROTEAN® TGX™ Precast Gels ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted proteins were analysed by 4—15% sodium dodecyl sulfate polyacrylamide (SDS-PAGE) gel electrophoresis (Mini-PROTEAN® TGX™ Precast Gels by Bio-rad) using broad range molecular weight markers (PageRuler™ Plus Prestained Protein Ladder ...
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bioRxiv - Genetics 2019Quote: ... Total protein was quantified using the BCA assay and 25-50μg of protein in 1x Lamelli Buffer with β-mercaptoethanol was loaded onto 4-15% TGX protean gels (Bio-Rad, Hercules, CA). The protein was transferred to a .2μm PVDF membrane by wet transfer ...
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bioRxiv - Cancer Biology 2021Quote: The protein extracts from the cells (40 μg) were subjected to electrophoresis in SDS-PAGE on 4–15% precast protein gels (Bio-Rad, Hercules, CA), separated and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: A sample of purified basal bodies was run briefly into a 4-20% Mini-PROTEAN® TGX Stain-Free™ gel (BIO-RAD), which was then stained with InstantBlue™ before the gel section containing the sample was excised and sent for analysis at the BSRC Mass Spectrometry Facility (University of St Andrews).
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bioRxiv - Cell Biology 2021Quote: ... Forty µg of denaturated proteins were subjected to a Western blot analysis after 4–15 % MiniPROTEAN® TGX StainFree™ gel electrophoresis (Bio-Rad) followed by probing antibodies against TLR4 or β-Actin (TLR4:1:400 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were heated 5 min at 95°C and separated on 4-20% Mini-PROTEAN® TGX Stain-Free™ Precast gels (Bio-Rad) or on home-made 7% polyacrylamide gel for OPA1 immunodetection ...
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bioRxiv - Molecular Biology 2020Quote: ... and the sample incubated at 90°C for 5 min and resolved by electrophoresis through a 4–20% gradient polyacrylamide/SDS gel (Bio-Rad Laboratories, 5671085). Next ...
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bioRxiv - Microbiology 2022Quote: ... beads were removed via a magnetic rack and proteins loaded on a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad). Blotting was performed using the Trans-Blot Turbo Mini 0.2 μm Nitrocellulose Transfer Pack (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was loaded from each sample into separate wells of a 4-15% polyacrylamide gel (Bio-Rad Laboratories, 456-8084). Electrophoresis was performed in 1x Tris/Glycine Buffer (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... 20-30 μg of protein extract was loaded onto 4 to 20% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Bio-Rad, Hercules, CA) and transferred to Immobilon-P PVDF membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: REST-FL was purified in two-step chromatography and carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... 10 ug of protein was loaded onto precast gel (4561095, 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Bio-Rad, USA) and resolved at 200 volts for 30-35 minutes ...
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bioRxiv - Genetics 2022Quote: ... Approximately 20 μg of each sample were loaded on a 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad cat no. 4561094). Proteins were transferred to a Nitrocellulose Membrane (Bio-Rad cat no ...
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bioRxiv - Plant Biology 2022Quote: ... and the total soluble protein extracts (25 μl or 40 μl per well) were separated on 4-20% Mini-PROTEAN TGX precast polyacrylamide gels (Bio-Rad, Hercules, CA) and subsequently transferred to a 0.45 μm nitrocellulose membrane (GVS ...
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bioRxiv - Microbiology 2022Quote: ... and mixed 1:1 with 2X Laemmli buffer followed by SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels and staining with Coomassie Brilliant Blue R-250 (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Microbiology 2022Quote: ... Marcy l’Etoile, France), sheep blood agar with colistin and nalidixic acid (CNA, bioMérieux) and chromogenic agar (Uriselect 4, Bio-Rad, Marnes-la-Coquette, France). Bacterial growth was determined after 18 h of incubation at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% Criterion TGX precast Midi protein gel (Bio-Rad, Cat#5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lysates were loaded onto 4-12% Criterion XT precast gels and resolved by SDS-PAGE under reducing conditions (Bio-Rad Laboratories, Hercules, CA). The proteins were then electro-blotted onto a 0.45 µm nitrocellulose membrane ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins immunoprecipitated with anti-FLAG antibody were separated by SDS-PAGE (4-15% Mini-PROTEAN®TGX™ Precast Protein Gels (Bio-RAD)) ...
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bioRxiv - Neuroscience 2022Quote: Protein sample was loaded into each well of a 4-20% Mini-PROTEAN TGX Precast protein 15-well gels (Bio-Rad, cat#4561096). Protein was transferred using transfer buffer (20% MeOH in TBS ...
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bioRxiv - Pathology 2024Quote: ... 15 µg of proteins were resolved on Criterion TGX Stain-Free 4-15%,18 and 26-well gel (Bio-Rad Laboratories, #5678084, #5678085) in a Criterion large format electrophoresis cell (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... 4 µl of the diluted cDNA was added to a Master mix containing 4 µl SsoAdvanced Universal SYBR® Green Supermix (Bio-rad #1725270) and 0.5 µl of a 10 µM stock of each Forward and Reverse primer (Table 5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... A total of 20 μg of protein was separated using a 4%–15% Criterion™ XT tris-glycine protein gel (BioRad, United States) and then transferred to nitrocellulose membrane (BioRad ...
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bioRxiv - Physiology 2024Quote: ... Then we separated proteins into 4-15% gradient SDS-PAGE gels and transferred them to PVDF immuno-blot membranes (Bio-Rad, Hercules, CA). The primary antibodies S6 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: Nuclear lysates of inputs and affinity-purified elution were loaded onto 4-15 % Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD, Cat# 5678085) and transferred to nitrocellulose membranes which were subsequently blocked by 5% BSA for 1Chour at RT ...
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bioRxiv - Immunology 2024Quote: ... of cells lysates was subjected to electrophoresis on a sodium dodecyl sulfate (SDS) 7.5% or 4-20% polyacrylamide gel (4561024 or 4561095, respectively, Bio-Rad, California, United States). Proteins were semi-dry transferred to PVDF membranes (1704159SP5 ...
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bioRxiv - Molecular Biology 2024Quote: ... Twenty micrograms of whole cell extracts were resolved on 4−12% SDS-polyacrylamide gels and transferred onto Ready gel blotting sandwiches immune-blot PVDF membranes (Biorad, Hercules, CA, USA) using a XCell II™ Blot Module (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μg protein were subjected to electrophoretic run using a 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories), transferred to PVDF membranes using the Trans-Blot® Turbo™ Midi PVDF Transfer Packs (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: Proteins within tailocins were separated using a 4-20% sodium dodecyl sulfate-polyacrylamide gradient gel (Mini-PROTEAN TGX Stain-Free Precast, Bio-Rad Hercules, USA) electrophoresis (SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2024Quote: ... for DpnII digested DNA or primers 3 and 4 (table S2) for NlaIII digested DNA on a thermal cycler (Bio-Rad C1000 Touch). PCR amplified samples were purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel ...
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bioRxiv - Genetics 2024Quote: ... were loaded in parallel with with pre-stained molecular weight markers and resolved by SDS-PAGE in premade 4–15% gradient Criterion gels (BioRad, Hercules, CA, USA). Resolved tissue homogenates were transferred onto polyvinylidene difluoride (PVDF) ...
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bioRxiv - Immunology 2024Quote: ... and lysates of equal concentration were prepared and run by SDS-PAGE on Bio-Rad Criterion 4–15% 26-well gradient gel (Bio-Rad, CAT#5678095). Gels were transferred using the Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-20 µl of the cell lysates were separated on 4-15% Mini-PROTEAN® TGX™ Stain-FreeTM Protein Gels from Bio-Rad and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... and boiled at 95 °C for 5 min before loading and running in 4-15% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad, Cat# 4561083) with Precision Plus Protein Dual Color Standards (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The equivalent of 1.107 parasites were loaded per lane in a 4–20% Mini-PROTEAN® TGX Stain-Free™ gel (Bio-rad, 4568096) and proteins were separated and transferred onto a nitrocellulose membrane using the transblot semi-wet transfer system (Biorad) ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µL of protein lysate was then loaded into 4–20% Criterion™ TGX Stain-Free™ precast gels (Bio-Rad, Cat. #5678094). Proteins were separated at 200V for 45 min and then transferred to a low-fluorescence PVDF membrane at 25V for 30 min using the Trans-Blot Turbo Transfer System and Kit (Bio-Rad ...