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Citations for Bio-Rad :
301 - 350 of 7734 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Microbiology 2020Quote: ... Samples were boiled at 100°C for 5 min prior to SDS-PAGE separation on 12% acrylamide Mini-protean TGX precast gels (Bio-Rad Laboratories, US) and Western blot analysis.
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bioRxiv - Immunology 2020Quote: ... plates were washed 5 times with 200 μl of 1X PBS per well and developed with 100 μl of TMB per well (Bio-Rad, #R8/R9) for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]). After removing the blocking buffer and re-applying PAP pen ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified on P-30 spin columns (Bio-Rad). Chemically synthesized oligoribonucleotides A27 and U18 were kind gifts of Prof ...
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bioRxiv - Plant Biology 2021Quote: ... 30 μl of SureBeads (Protein A Magnetics Beads, BioRad) and 1/1000 of Histone 3 (H3 AS10 710 ...
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bioRxiv - Plant Biology 2020Quote: ... RNase-free Bio-Spin columns P-30 (Bio-Rad) were used for radiolabeled RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 A for 30 min (BioRad Trans-Blot Turbo). The transfer membrane was air-dried and then washed in PBS (10 mM phosphate buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 A for 30 min (BioRad Trans-Blot Turbo). The transfer membrane was washed in PBS (10 mM phosphate buffer ...
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bioRxiv - Biophysics 2022Quote: ... Acrylamide/Bis-acrylamide (30%, 29:1, 1610156, Bio-Rad), N,N,N’,N’-Tetramethylethylenediamine (TEMED ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... stained for 30 min with GelCode (Bio-Rad Laboratories) and washed overnight in milliQ before manually slicing each lane into 5 equal slices ...
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bioRxiv - Genomics 2024Quote: ... buffer exchange using P-30 columns (Bio-Rad Laboratories) was performed ...
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bioRxiv - Biochemistry 2024Quote: ... then 30 µl of 2× Laemmli sample buffer (BioRad) and 1 µl of proteinase K (Thermo Scientific ...
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bioRxiv - Physiology 2024Quote: ... (BioRad, Hercules, CA), appropriate forward and reverse primers ...
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bioRxiv - Bioengineering 2020Quote: ... quantification was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Chromatographic separation was performed using a Rezex Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 55 °C ...
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bioRxiv - Microbiology 2020Quote: ... Lactose and salts were removed by gel filtration chromatography over a BioRad P2 column (100 cm x 16 mm, Bio-Rad, Hercules, CA. USA) using a semi-automated Fast Protein Liquid Chromatography (FPLC ...
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bioRxiv - Microbiology 2022Quote: ... Vesicle preparations were treated with 6xSDS loading buffer and boiled for 10 min at 100°C and run on a 10% SDS-PAGE gel (Bio-Rad Laboratories, Hercules, CA), stained for 15 min with Coomassie Brilliant Blue Stain ...
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bioRxiv - Immunology 2022Quote: ... cell debris was removed by centrifugation and clear supernatants were diluted 10-fold and subjected to anion exchange chromatography on gravity fed columns using AG-1 9 8 resin (formate form, 100–200 mesh size, Bio-Rad Laboratories, Hercules, CA). Fractions containing inositol mono ...
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bioRxiv - Synthetic Biology 2020Quote: ... Chromatographic separation was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... Chromatographic separation was performed using a Bio-Rad Fast Acid Analysis HPLC Column (100 x 7.8 mm, 9 μm particle size; CAT#: #1250100, Bio-Rad Laboratories, Inc., Hercules, CA) at 65 ℃ ...
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bioRxiv - Genetics 2024Quote: ... complementary DNA was made from approximately 100 ng of RNA using the iScript Reverse Transcription Supermix for quantitative PCR with reverse transcription (Bio-Rad, Hercules, CA, USA). Expression levels of β-globin transgene and α-globin mRNA were quantified with a Bio-Rad QX200 ddPCR machine and QuantaSoft software (v.1.7 ...
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bioRxiv - Plant Biology 2023Quote: ... Seed storage proteins were visualized from 100 µg of seed material on a 12% SDS-PAGE compared to a Precision Plus Dual Color Protein Standard (Bio-Rad, Hercules, CA, USA). Gels were stained for 1 hr using a 0.1% Coomassie Brilliant Blue ...
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bioRxiv - Physiology 2023Quote: Enzymes were extracted using a phosphate-based buffer (50 mM KH2PO4, 1 mM EDTA, 0.05 % Triton X-100) and quantified by Bradford assay (Bio-Rad #5000006, Bio-Rad, Hercules, CA, USA). Citrate synthase activity was measured spectrophotometrically as previously described106.
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using 100 ng of purified total RNA in a 20 μL volume reaction with an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA), as described in (52) ...
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bioRxiv - Cell Biology 2024Quote: ... The lysates were centrifuged at 16,100 rcf for 15 minutes at 4 °C and protein concentration was quantified using the DC™ Protein Assay kit (Bio-Rad, Hercules, CA).
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bioRxiv - Systems Biology 2021Quote: ... incubated 5 min at 65 °C and separated by 12% SDS-PAGE (65) in the Mini Protean 3 Apparatus (BioRad Laboratories, Hercules, CA, USA). The final concentration of proteins was 10 μg per lane ...
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bioRxiv - Molecular Biology 2021Quote: ... 300nM final volume of each forward and reverse primer pair, 5 μL SYBR Green Supermix (50% total volume of reaction, rxn) (Bio-Rad Inc. Hercules, CA), and nuclease-free water (to make a final reaction volume of 10 μL) ...
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bioRxiv - Cell Biology 2021Quote: The supernatants of cell patches were collected after culturing under normoxic or hypoxic (5% O2) conditions and analysed using a fluorescence-dyed microsphere-based immune assay (Bio-Rad Laboratories, Hercules, CA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Biophysics 2022Quote: ... The re-suspended DNA droplet solution was then denatured at 95 °C for at least 5 min on a C1000 Touch™ Thermal Cycler from Bio-Rad (CA, USA). The aqueous solution consisted of 25% v/v SUV solution (containing 8mM lipids as prepared above ...
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bioRxiv - Synthetic Biology 2021Quote: ... A 250 μL suspension of cells and 500 ng of double-digested (XbaI and KpnI) vector plasmid were incubated for 5–10 min on ice in a 4-mm Gene Pulser®/MicroPulser™ cuvette (BioRad, Hercules, CA). GenePulser XCellTM (BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... and the miR200c primers (upstream primer, 5’- TAATACTGCCGGGTAATGATGGA-3’) (Eurofins Genomics) on the CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). U6 primers (TAKARA ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression for the 5 marker genes was quantified by qPCR using SYBR Green PCR Master Mix (Bio-Rad Laboratories Inc., Hercules, CA, USA), in three independent samples per treatment ...
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bioRxiv - Biochemistry 2023Quote: ... Then the membrane was incubated with mouse monoclonal antibodies against PAR for 60 min in PBS-T/5% milk followed by incubation with the secondary anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad, Hercules, CA, USA) for 60 min in the PBS-T/5% milk solution ...
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bioRxiv - Microbiology 2023Quote: ... followed by 40 cycles (95°C for 5 seconds, 60°C for 40 seconds) on a CFX96 Real-Time PCR System (Bio-Rad, Hercules, CA, USA). The housekeeping gene GAPDH (PY17X_1330200 ...
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bioRxiv - Microbiology 2024Quote: ... 250 μL of the mixed cell suspension was chilled on ice for 5-10 minutes in a 4-mm wide cuvette compatible with the Gene Pulser®/MicroPulser™ (BioRad, Hercules, CA). The cell mixture was electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Immunology 2024Quote: ... The 1:10 diluted plasmid was combined with C41 cells (∼3 x 109 competent cells) and incubated on ice for 5 min followed by electroporation using a Bio-Rad Electroporator (Bio-Rad, Hercules, CA, USA). The transformation was incubated at 37°C in LB media for 1 hr prior to spreading onto LB agar plates containing kanamycin ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted at a concentration of 5 ng/µl and 2 µl were loaded in duplicate in a 96-well qPCR plate with the reaction mix comprising 5 µl of Sybr Green master mix (Bio-Rad, Hercules, CA, USA), 2 µl of water and 1 µl of primer mix containing forward and reverse primers for STAT3 and P53 ...
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bioRxiv - Molecular Biology 2024Quote: ... a mixture of 250 μL of this cell suspension and 1000 ng of a vector plasmid digested with XbaI and KpnI enzymes was chilled on ice for 5–10 minutes within a 4-mm width cuvette compatible with the Gene Pulser®/MicroPulser™ system (BioRad, Hercules, CA). The cell-DNA mixture was then electroporated using the GenePulser XCell™ device (BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... Non-specific binding sites were blocked at room temperature for 1 hour with 5% (w/v) Blotting-Grade milk (Bio-Rad Laboratories, CA, USA) in Tris-buffer saline (Boston Bio Products ...
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bioRxiv - Biochemistry 2022Quote: 5 µL aliquots of the CRISPR complex solution were buffer exchanged into 100 mM ammonium acetate using Biospin P-6 gel columns (Bio-Rad Laboratories Inc., Hercules, CA) prior to native mass spectrometry ...
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bioRxiv - Biochemistry 2022Quote: LiRecT protein was buffer exchanged into 100 mM ammonium acetate pH 7 (unadjusted) using Micro BioSpin P6 spin columns (Bio-Rad Laboratories, Hercules, CA, USA). All ssDNAs were dialyzed into 100 mM ammonium acetate with Pierce 96-well microdialysis devices with 3.5K MWCO (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated overnight at 4°C with antibody cocktail consisting of rat anti-F4/80 (1:100) (catalog #: MCA497; Bio-Rad Laboratories; Hercules, CA, USA), rabbit anti-vimentin (1:100) ...
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bioRxiv - Cell Biology 2021Quote: ... A 5-μL aliquot of the pre-amplified reaction was analyzed by qPCR using SsoFast EvaGreen Supermix (Bio-Rad Life Science, Inc., Hercules, CA, USA) and an Applied Biosystems 7900HT qPCR instrument (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... exposed to enhanced chemiluminescence plus west pico for 5 min and autoradiographed in a BioRad Gel-Doc System (Bio-Rad Laboratories Inc., Hercules, CA, USA). The loading consistency was verified utilizing the house-keeping proteins (Rab11 and β-Actin ...
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bioRxiv - Plant Biology 2022Quote: ... Amplification of target amplicons with the addition of 5’ XbaI and 3’ BamHI restriction enzyme digestion sites was carried out on a Bio-Rad T100 thermocycler (Bio-Rad Laboratories, Hercules CA, USA) via Bio-Rad iProof High Fidelity DNA Polymerase using manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Electroporation was carried out by mixing 45 µl of competent cells with 100-200 ng of plasmid and subjecting the cells to a short 5 ms electric pulse under a 12500 V/cm electric field in a Gene Pulser II® (Bio-Rad, Hercules, CA, USA) (2500 v ...
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bioRxiv - Molecular Biology 2022Quote: ... and buffer exchanged by passing through P-30 columns (Biorad). 3’ RNA adaptor (/ 5Phos/NNNNNNNNGAUCGUCGGACUGUAGAACUCUGAAC/3InvdT/ ...
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bioRxiv - Immunology 2024Quote: ... for 30-60 minutes before gravity filtration (Bio-Rad, #7321011). The resin was washed with ∼5-10 bed volumes of PBS before adding 20 mls of PBS containing 200 mM imidazole (pH ∼7.4 ...