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Citations for Bio-Rad :
251 - 300 of 7734 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 μg of total protein was loaded onto 8 to 15 % SDS-PAGE mini gels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Bioengineering 2022Quote: ... pH 8.3 at 100 V for 2 h at 2-8 °C using PowerPac Basic setup (Bio-Rad Laboratories Inc., Hercules, CA). Post-electrophoresis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total protein amount of 100 μg for each sample was separated on 12% Criterion gels (Bio-Rad Laboratories, Hercules, Ca, USA), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... 10μl of EMSA binding reactions were loaded on 5% nondenaturing polyacrylamide Mini-PROTEAN TBE gels (Catalog #4565015, Bio-Rad Laboratories, Hercules, CA) for electrophoretic analyses ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each PCR reaction (10 μL final volume) contained 5 μL SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, CA), 100 ng of cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined by BCA protein assay (Pierce) and boiled for 5 minutes with Laemmli Sample Buffer (Bio-Rad, Hercules, CA). Proteins were separated on acrylamide gels and analyzed by Western blot using primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated to 95°C for 5 min and then analyzed on a 4-12% gel (Bio-Rad Laboratories, Hercules, CA) using SDS running buffer ...
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bioRxiv - Immunology 2020Quote: ... and 40ng of RNA for lung or 5-10ng of RNA for cells was converted to cDNA with iScript (Biorad, Hercules, CA). Real-time PCR was done on the cDNA with SYBR green master mix (Biorad ...
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bioRxiv - Molecular Biology 2023Quote: ... 25-40μg of protein was run on 7.5-15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred using the Trans-Blot Turbo system (Biorad, Hercules, CA) to nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting pellet was suspended in the lysis buffer and loaded onto a 5 ml IMAC column (Bio-Rad, CA, Hercules, USA) pre-equilibrated with a buffer A (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The samples were run with 5 mM H2SO4 at 0.6 mL/min on an Aminex HPX-87H column (Biorad Inc., CA, USA) at 50℃ ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The clarified lysate was loaded with a peristaltic pump (Hei-FLOW 06, Heidolph, Germany) on prepacked nickel-NTA columns (5 mL Nuvia IMAC Ni-Charged, Biorad, USA-CA) that were equilibrated before with buffer A ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then blocked for 1 hour in 5% non-fat milk in PBS containing 0.1% Tween 20 (Bio-Rad, Hercules, CA) at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... The qPCR reaction was carried out on 2μL of 1/5-diluted cDNA using 2X SSoAdvance SYBR Super Mix (Bio-Rad, Hercules CA) with locus-specific primers (Table S2).
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bioRxiv - Bioengineering 2024Quote: ... The libraries were diluted to 5 nM concentration and further quantitated by qPCR on a Bio-Rad CFX Connect Real-Time System (Bio-Rad, CA).
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bioRxiv - Bioengineering 2024Quote: ... 700 μL of cell culture supernatant was filtered through a 0.2-micron filter and run with into 5 mM H2SO4 at 0.5 mL/min on an Aminex HPX-87H column (Biorad Inc., CA, USA) at 15 ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction mixture was incubated for 30 minutes at 30°C and stopped by adding 12 μl of Laemmli sample buffer (Biorad). After HMT reactions ...
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bioRxiv - Biophysics 2021Quote: ... at 200V for 30 min (Bio-Rad 1645050). Protein was then transferred to a PVDF membrane using iBlot™ 2 Transfer Stacks (Invitrogen IB24002 ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 μl 2x Laemmli Sample Buffer (Bio-Rad) with 5% b-mercaptoethanol were added to the sample followed by incubation at 95°C for 5 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... 30% (19:1 monomer:bis) acrylamide solution (Bio-Rad) and SYBR Gold (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... 30-37.5 v/v% 40% acrylamide solution (Biorad), 0.2 w/v% sodium dodecyl sulfate (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... 30-37.5 v/v% 40% acrylamide solution (Biorad), 0.2 w/v% sodium dodecyl sulfate (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... except that blocking was done with phosphate-buffered saline plus 0.1% Triton X-100 (PBS-T) containing 5% nonfat dry milk (Biorad Blotting grade Blocker) and 10 mM NaN3 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then centrifuged (2500 × g for 5 min) and pellets were resuspended in 100 μL of PAGE Laemmli Sample Buffer (Bio-Rad, 1610747) mixed with 50 μL TE 1X pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were eluted from sepharose beads by boiling at 95 - 100 °C for 5 min with 40 µl sample buffer diluted from 4x sample buffer (Bio-Rad, 1610747) with Lysis Buffer ...
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bioRxiv - Zoology 2024Quote: Genomic DNA was extracted from the right hind leg of individual worker bee using a 5% (w/v) Chelex solution (Chelex®100; BIO-RAD), as followed by Walsh ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction mix was incubated for 30 mins at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... 60°C for 30 sec and 72°C for 30 sec on a Bio-Rad CFX96 qPCR cycler (Bio-Rad, Germany).
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bioRxiv - Microbiology 2022Quote: ... Protein concentration was calculated against a BSA standardized curve using with the Pierce BCA Protein Assay Kit and a total of 30 μg per sample was mixed with resuspended in 30 μL of 4x laemmli buffer (Bio-Rad) supplemented with 5% 2-mercaptoethanol and boiled for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Antibiotic disk content used in the assays were cefotaxime 30 μg and amoxicillin + clavulanic acid 30 μg (20 μg + 10 μg; all from Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories, Hercules, CA). Separated proteins were transferred onto nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A total of 100 μg brain lysate was run on a Bio-Rad Mini-Protean TGX 4-20% polyacrylamide gel (4561094, Bio-Rad, Hercules, CA) at 200V for approximately 40 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... This stock solution was then diluted (at concentrations of 175, 150, 125, 100, 75, 50 μg/mL) and mixed with 4X Laemmli buffer (Biorad, Hercules, CA, USA) containing 1 % β-mercaptoethanol for SDS-PAGE calibration ...
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bioRxiv - Microbiology 2024Quote: ... Then 300 ng of plasmids were transformed into 100 μl of competent cells using the bacteria program on the MicroPulser Electroporator (Bio-Rad, CA, USA). After 1 h recovery culture at 37°C in 500 μl of LB ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... The suspended cells were then diluted 1:100 in PBS and counted using a TC20 automated cell counter (Bio-Rad Laboratories, Hercules CA). For the first vaccination with cda1Δ2Δ3Δ (given to CBA/J ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were separated in sodium dodecyl sulfate – 8% polyacrylamide gel by electrophoresis at 100 V for 2 h (Bio-Rad, Hercules, CA, USA). Wet transfer to a PVDF membrane (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... 40 µL of the cell solution with 1 µL DNA (100 ng/µL in Tris-Buffer) was added to a chilled cuvette and pulsed using a Bio-rad Genepulser (Bio-Rad, CA, USA) for 5.7 ms (2000 V ...
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bioRxiv - Cell Biology 2020Quote: ... The protein samples were mixed with LDS sample loading buffer at 4X concentration followed by addition of beta-mercaptoethanol (5%) (Bio-Rad, Hercules, CA). The resultant mixture was denatured at 95°C for 9 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Real-Time reactions were performed in triplicates using KAPA SYBR FAST qPCR Kit (2X) on QuantStudio 5 Real-time PCR system (Bio-Rad, CA, USA) following the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2020Quote: ... was electroporated into cells at 5×106 cells/0.4 ml under conditions of 220V and 950 µF using a Gene Pulser™ (Bio-Rad, Hercules, CA) and plated on DMEM containing 10% FBS.
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bioRxiv - Cell Biology 2020Quote: ... transferred to 0.2 μm pore-size PVDF membranes, and blocked for 1 hour in blocking buffer (5% milk, 0.1% Tween-20 in 1x TBS (Bio-Rad, Hercules, CA, USA)) ...
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bioRxiv - Biochemistry 2021Quote: ... were co-electroporated with 1.5 x 107 KH2 ES cells at 400 V and 125 μf using two pulses in a Gene PulserII (Bio-Rad, Hercules, CA). Recombination between FRT sites such that the entire plasmid is inserted within the PGK promoter and the ATG initiation codon upstream and in frame with the hygromycin resistance gene was used to select correctly targeted cells ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by a final extension step at 68 °C for 5 min on the T100™ thermal cycler (Bio-Rad, Hercules, CA, USA). During protocol optimization ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Neuroscience 2023Quote: ... and 5 U/mL aprotinin) and the protein concentrations in the lysates were determined using a BioRad protein assay kit (BioRad Laboratories, Hercules, CA). Protein samples were subjected to SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... were detected as dark spots after a 10-min reaction with 5-bromo-4-chloro-3-idolyl phosphate and nitro blue tetrazolium using an alkaline-phosphatase-conjugate substrate (Bio-Rad, CA, USA). SFUs were counted using the AID ELISpot Reader System (Autoimmun Diagnostika) ...
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bioRxiv - Cell Biology 2022Quote: ... in 1× TBS for 5–10 min at room temperature to visualize DNA and mounted with FluoroGuard anti-fade reagent (Bio-Rad, Hercules, CA).
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...