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Citations for Bio-Rad :
101 - 150 of 7734 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... IEs were shot twice using the PDS-100/He gene gun (Bio-Rad, Hercules, CA) with rupture pressure of 1100 psi ...
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bioRxiv - Neuroscience 2024Quote: ... serum samples were diluted 1:5 with Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) and heated at 100 °C for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were subsequently separated on a pre-run 5% TBE gel (Bio-Rad; Hercules, CA). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... All samples were acquired on a ZE5 5-laser cell analyzer (Biorad laboratories, Hercules, CA) and analyzed with FlowJo software (Tree Star ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 40 cycles of 95 °C for 5 sec and 60 °C for 30 sec using the CFX 96 real-time systems (Biorad). The 2−ΔΔCt method was used to analyze the data (Schmittgen and Livak ...
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bioRxiv - Microbiology 2021Quote: ... Each sample (30 µg) was run on a 14% SDS-PAGE gel and transferred to a nitrocellulose membrane (Bio-Rad, CA). HTLV-1 Gag expression in cells and VLPs in the cell culture supernatant was detected using a mouse monoclonal antibody HTLV-1 p24 (6G9 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown at 30°C for 3-6 days and images captured using a Chemidoc XRS+ imager (BioRad, Hercules, CA). All images were evenly adjusted in Photoshop (Adobe Systems Incorporated ...
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bioRxiv - Cancer Biology 2024Quote: ... 30 µg of protein lysate were then run per lane on a 10% polyacrylamide gel (Bio-Rad Laboratories, Hercules, CA, USA) and transferred to a PVDF membrane (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μg of each sample was boiled with 4x Laemmli Sample Buffer containing 10% 2-mercaptoethanol (#1610747, BioRad, Hercules, CA, USA) and run on a Mini-PROTEAN® TGX™ Precast Gel (#4561083 ...
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bioRxiv - Biophysics 2023Quote: ... The solution was allowed another 30 min of incubation before addition of 40 mg of wet BioBeads (Bio-Rad, Hercules, CA) per mL of LUVs solution to slowly remove the detergent over 3 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein samples of 25–30 μg were loaded onto a 10% SDS‒PAGE gel (Bio-Rad Laboratories Inc., Hercules, CA, USA) and electrophoresed at 80 V for 2 hours ...
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bioRxiv - Immunology 2021Quote: We mixed 500 μg of extracted cell lysates and 100 μL of patients’ sera with 100 μL of SureBeads™ Protein G Magnetic Beads (Bio-Rad, Hercules, CA) and added phosphate-buffered saline containing 0.2% of Tween 20 (PBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplification was carried out using of T 100 Thermal Cycler (Bio-Rad, Hercules, CA, USA) following the profile (39) ...
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bioRxiv - Developmental Biology 2020Quote: ... Affigel Blue Beads (1537301, 250-300 µm diameter, 50-100 mesh, Bio-Rad, Hercules, CA, USA) were washed with PBS and soaked in either 10 mM SB431542 ...
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bioRxiv - Microbiology 2023Quote: ... The medium was treated with 10 g·l-1 of BT Chelex 100 resin (BioRad, Hercules, CA) for 2 h and then filtered using a vacuum-driven 0.22 µm filter (Olympus plastics ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in 100 µl Opti-MEM in an electroporation cuvette (4 mm) (Bio-Rad, Hercules, CA) and 100 µl labeled RNPs were added ...
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bioRxiv - Neuroscience 2021Quote: Probed cDNA amplification was conducted using 5 μL of SYBR Green Supermix (BioRad, Hercules, CA, USA), 0.5 μL primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Reactions consisted of 5 μL of SsoAdvanced Universal SYBR® Green Supermix (BioRad, Hercules, CA, USA), 0.25 μL of forward and 0.25 μL of reverse primer at 10 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... p53 and β-actin primary antibodies were diluted in 5% milk (Bio-Rad, Hercules, CA, USA) in TBST ...
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bioRxiv - Microbiology 2022Quote: ... 5 mM MgCl2 and 0.1% NP40) separated through a 12% polyacrylamide gel (Bio-Rad, Hercules, CA) by electrophoresis (SDS-PAGE ...
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bioRxiv - Physiology 2024Quote: ... nitrocellulose membranes were blocked in 5% blotting grade blocker nonfat dry milk (Bio-Rad, Hercules, CA) in TBS (wt/vol ...
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bioRxiv - Cell Biology 2023Quote: ... Equal aliquots of protein were loaded onto 5-20% polyacrylamide gels (Bio-Rad, Hercules, CA, USA) run for 80 minutes at 50V then blotted onto PVDF membrane (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10 x BN loading dye (5% Coomassie Blue R-250 (Bio-Rad Laboratories, CA) in 500 mM 6-aminohexanoic acid ...
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bioRxiv - Neuroscience 2024Quote: ... before being air-dried overnight and then blocked in 5% skim milk (Bio-Rad, Hercules, CA) in phosphate buffer saline containing 0.1%Tween®20 (PBST)(Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... which were then blocked in 5% blocking grade skim milk (170–6404, Bio-Rad, Hercules, CA). Primary antibody was used at a dilution of 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... followed by centrifugation for 5 minutes at 12,000 rpm at 4°C (Bio-Rad, Hercules, CA). After centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... gel containing 8 M urea (48% (w/v)) and 1X TBE was prerun for 30 min at 100 V in a vertical mini- PROTEAN tank (Bio-Rad). Gels were flushed and loaded with 10 μg of total RNA in 2X TBE/Urea sample buffer (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... in a 100 μL reaction for 2 hours at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad). The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher) ...
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bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Bioengineering 2020Quote: ... followed by 40 cycles of 95°C for 5 s and 60°C for 30 s (Bio-Rad CFX manager 3.1). Quantification of mRNA levels of different genes was performed using the 2−ΔΔct method ...
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bioRxiv - Biochemistry 2024Quote: ... Images were collected following 5 days of incubation at 30 °C with a Bio-Rad Chemidoc MP Imaging System (Bio-Rad) and analyzed using the ImageJ software (50).
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 30 mg BioBeads (BioRad) and TEV protease (laboratory stock ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with C-kit variant 1 forward primer 5’-CAACAAAGAGCAAATCCATCCC-3’ and reverse primer 5’-CATCACAATAATGCACATCATGCC-3’ with iQ SYBR Green supermix (BioRad, Hercules, CA, Cat No. 1708882). The PCR program was as follows ...
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Protonation/deprotonation-driven switch for the redox stability of low-potential [4Fe-4S] ferredoxinbioRxiv - Biochemistry 2024Quote: ... applied to an anion exchange column (Mono Q HR 5/5, Cytiva) chromatography using the NGC Quest 10 system (Bio-Rad Laboratories, Inc. Hercules, CA).
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bioRxiv - Biophysics 2021Quote: The removal of DDM in the protein solutions was done by fixing the protein concentration at 1 mg/ml and using 30 mg of BioBeads SM2 (Bio-Rad, CA) per mg of protein ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.5 was heated from 15 ºC to 90 ºC with 0.5 ºC increment every 30 seconds on an iCycle iQ5 Real Time Detection System (Bio-Rad, Hercules, CA). The normalized fluorescence data was plotted against temperature (53).
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was purified with DNase and incubated at 37°C for 30 minutes using a thermocycler (Bio-Rad, Hercules, CA, USA). cDNA was synthesized using an iScript kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... and heated at 50 °C for 30 min prior to loading on a 10% criterion Tri-HCl gel (Bio-Rad, Hercules, CA). Samples were then transferred onto PVDF membranes (Millipore Sigma ...
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bioRxiv - Physiology 2021Quote: ... 95°C for 20 s followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Cancer Biology 2022Quote: Whole cell extracts of NB4 and HT93 were prepared using urea lysis buffer and 30-60μg total protein was loaded on a denaturing 12%-polyacrylamide gel self-cast gel (Bio-Rad Laboratories, CA). Proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Zoology 2023Quote: ... followed by 40 cycles at 95°C for 3 s and 60°C for 30 s using a real-time thermal cycler (CFX Connect; BioRad, Hercules, CA).
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bioRxiv - Neuroscience 2024Quote: ... 30 µg of protein was loaded into each well of a 4-15% Stain-Free polyacrylamide gel (Bio-Rad, Hercules, CA, USA) and resolved via SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... and 30 µg of each sample were loaded and run on Mini-PROTEAN TGX 4-15% acrylamide gels (Bio-Rad, Hercules, CA). Proteins were transferred to PVDF membranes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... DNA was extracted using 150 μL 5% Chelex 100 resin (Bio-Rad Laboratories, Hercules, United States) according to methods described previously (Lee et al 2012) ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted by mixing a small algal sample with 5% Chelex-100 resin (BioRad), incubated at 95°C for 20 min and 4°C for 20 min ...
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bioRxiv - Plant Biology 2021Quote: ... Plant leaf protein samples (30-100 µg) were run on a 10% Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-Rad) and transferred to PVDF membranes ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were blocked with Bovine Serum Albumin (BSA) in DPBS for 30 min and then incubated with anti-His6 antibody (1:100, Bio-Rad, MCA1396) for 2 h at 25°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and electroporated (square wave, 6 pulses, 30 V, 3 ms duration, 100 ms interval) using a gene Pulser XCell electroporator (Bio-Rad, 1652660). Zygotes were recovered and transferred to fresh KSOM drops and cultured overnight ...
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bioRxiv - Neuroscience 2021Quote: ... were mixed with filtered monomeric α-syn WT (30 μM) and ThT (10 μM) and were incubated at 55°C for 5 h in a PCR machine (Bio-rad, C1000) for amplification.[3] Fractions comprised of ≥ 76% of its original volume were mixed with α-syn and ThT to reach a final volume of 20 μL per reaction ...
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bioRxiv - Neuroscience 2020Quote: ... 100 microgram of RNA was reverse transcribed using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA), according to the manufacturer’s instructions ...