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Citations for Bio-Rad :
301 - 350 of 7689 citations for 6 oxo 1 phenyl 1 4 5 6 tetrahydro pyridazine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... for 1–3 hours at 100 V and then transferred to PVDF membrane (Bio-Rad) at 40 V for 2–8 hours ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg of lysate was heat denatured for 3 minutes at 95°C for 3 minutes and resolved on a pre-cast 4-15 % SDS-PAGE gels (Bio-Rad) at 200V for 45 minutes in 1X running buffer (25mM Tris pH 8.3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were mixed 1:4 with 4X Laemmli buffer (Bio-Rad, Lunteren, Netherlands) containing 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 4°C using the Mini Trans-Blot Cell (Bio-Rad). After the transfer ...
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bioRxiv - Genomics 2024Quote: ... and counted (1:4 dilution) with TC20 Automated Cell Counter (Bio-rad cat. #1450102). Cells were fixed using Parse Biosciences’ Nuclei Fixation Kit v1 (Parse Biosciences cat ...
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bioRxiv - Developmental Biology 2023Quote: ... All cDNA samples were diluted 1:5 and 1 μL was used in quantitative PCR (qPCR) reactions with iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124), according to manufacturer’s instruction and using the Applied Biosystems QuantStudio 7 flex qPCR System ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 1◻h incubation at 4◻°C with HRP-conjugated goat anti-mouse (1:5000 dilution in 1% YE in PBS-T, BioRad #170–6516) secondary antibody ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4°C and for 1 h at room temperature (RT) with secondary horseradish peroxidase conjugated antibodies (Bio-Rad, 1:3000). Membranes were then developed using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... This mixture was then incubated at 4 °C for 1 hour and further subjected to a 4-hour incubation with bio-beads (Bio-Rad, USA). After incubation ...
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bioRxiv - Biophysics 2021Quote: ... The uncoupled BG-GLA-NHS was removed by filtration on Micro Bio-Spin™ P-6 Gel Columns (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... approximately 250.000 cells were plated in a 6-well dish and transfected with a final concentration of 22.5 nM siRNA using siLentFect Lipid Reagent (Bio-Rad) accordingly to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... MtCoaBC and MsCoaBC were exchanged into NH4OAc (500 mM, pH 7.0) solution using Micro Bio-Spin 6 chromatography columns (Bio-Rad). A sample volume of 2.5 μL was injected into a borosilicate emitter (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... YpdA and YpdAC14A proteins were pre-reduced with 10 mM DTT followed by DTT removal with Micro Biospin 6 columns (Biorad). For the biochemical activity assays of the specific BSSB reductase activity ...
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bioRxiv - Biophysics 2019Quote: ... We then removed excess dye by four subsequent spins of the solution over Micro Bio-Spin 6 Columns (Bio-Rad) at 700 g for 2 min ...
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bioRxiv - Molecular Biology 2019Quote: ... EtOH precipitation was performed and DNA was additionally cleaned up on Micro Bio-Spin 6 chromatography columns in SSC (BioRad) as purity was essential for oxidation of hmC ...
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bioRxiv - Molecular Biology 2020Quote: DNA isolated from cells by phenol:chloroform isolation and ethanol precipitation was cleaned on Micro Bio-Spin P-6 SSC columns (Bio-Rad) according to manufacturer protocol ...
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bioRxiv - Biochemistry 2019Quote: ... The spin-labeled protein was further concentrated and free label was removed using Micro BioSpin 6 Chromatography Columns (Bio-rad) pre-equilibrated with buffer C.
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bioRxiv - Plant Biology 2020Quote: ... Captured elution fraction from the Strep-Tactin-affinity purification were loaded onto a anion exchange column (UNO Q-6, Biorad) with a flowrate of 4 ml/min ...
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bioRxiv - Biochemistry 2020Quote: ... and CyaY were buffer exchanged into 200 mM ammonium acetate (pH = 7.5) using Micro Bio-Spin 6 Columns (Bio-Rad). Instrument parameters were tuned to maximize transmission but simultaneously preserve the native-like state of proteins as determined by ion mobility ...
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bioRxiv - Neuroscience 2020Quote: ... IL-6) were quantified using a real-time PCR detection system and SsoAdvanced Universal SYBR Green Supermix kit (BIO-RAD). In brief ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing protein were pooled and desalted to remove imidazole by gel filtration on a 50 mL BioScale P-6 column (BioRad) using resuspension buffer as the eluant.
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bioRxiv - Biochemistry 2020Quote: ... the cleaved His6 tag was removed and the enzymes were buffer exchanged into 200 mM ammonium acetate (pH 7.5) using Micro Bio-Spin 6 columns (Bio-Rad). His6-affinity tagged HalA2 was likewise digested with thrombin to remove the His6 tag ...
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bioRxiv - Cancer Biology 2022Quote: Proteins were resolved in 6-8-10 or 12% polyacrilammide gels and transferred to PVDF (Bio-Rad, Hercules, CA,USA) or nitrocellulose membranes (GE Heath Care ...
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bioRxiv - Biochemistry 2022Quote: ... the enzymes were buffer exchanged into their respective reaction buffers above using a Micro Bio-Spin 6 size exclusion spin column (Biorad) to remove glycerol and other ion suppressing constituents ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to PVDF membrane at 4°C for 14.5 h at 30 V using the mini trans-blot module in transblot buffer (10 mM MES buffer pH 6 and 10% (v/v) methanol) according to the supplier’s instructions (Bio-Rad). The membrane was removed from the cassette ...
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bioRxiv - Biophysics 2021Quote: ... and 2 times the critical micelle concentration (CMC) of various detergents (namely LDAO and C8E4) using a Biospin-6 column (BioRad) and introduced directly into the mass spectrometer using gold-coated capillary needles (prepared in-house) ...
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bioRxiv - Developmental Biology 2019Quote: ... Alcian Blue was extracted by 6 M guanidine hydrochloride solution and measured by optical density at 600 nm (O.D. 600) using spectrophotometer (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... for 60 min at 37°C followed by purification on a Micro-Bio Spin P-6 Gel Column (Bio-Rad). 1 pmol of 5’-end-radiolabeled primer was used for each extension reaction ...
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bioRxiv - Neuroscience 2019Quote: ... Serum was isolated and IL-6 levels were measured using a Bio-Rad Luminex system (Bio-Rad, Laboratories, Hercules, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: APRT2-Ntag and APRT2-Ctag were buffer exchanged into a volatile buffer (200 mM ammonium acetate, pH 7.4) using a Micro Bio-Spin 6 device (Bio-Rad). Samples were loaded into pulled glass capillaries prepared in house ...
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bioRxiv - Immunology 2021Quote: ... before buffer exchange into 500 mM ammonium acetate solution (pH 7.5) using a Micro Bio-Spin 6 column (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... were exchanged from size exclusion buffer into 2 M ammonium acetate pH 7.4 via a single-pass through a Bio-Spin P-6 column (Bio-Rad). Buffer-exchanged protein was loaded into gold-coated glass capillaries (1.0 mm OD/0.75 mm inner diameter with filament) ...
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bioRxiv - Biochemistry 2023Quote: ... CsgA and CsgAslowgo samples were buffer-exchanged into MS-compatible buffer (150 mM Ethylene diamine diacetate pH 6.3, 10mM TMAO) using Micro Biospin 6 desalting columns (Bio-Rad) to a final protein concentration of 3-5 μM ...
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bioRxiv - Plant Biology 2023Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE (pH 8.0) buffer and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
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bioRxiv - Biochemistry 2023Quote: ... The oligomeric state of CsoSCA-MBP was determined from the Superose 6 Increase chromatogram and a calibration standard (Bio-Rad). Molecular weight of standard was as follows ...
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bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
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bioRxiv - Molecular Biology 2024Quote: ... The binding reaction was resolved on a 6% polyacrylamide gel in 0.5X TBE buffer (pH 8.0) and was semi-dry blotted (Transblot, BIO-RAD, USA) onto a positively charged nylon membrane (BrightStar ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated (4°C, overnight) with 5 % milk powder (BioRad) in TBST ...
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bioRxiv - Neuroscience 2022Quote: ... 5-20μg protein were loaded onto 4-20% SDS-PAGE gels (Biorad) and run at 100V ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ // Reverse: 5’-CGA AGG TGT GAC TTC CAT G-3’) and CFX96 Touch Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... 1 μL diluted cDNA (5 ng) in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each experimental sample (four source colonies ...
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bioRxiv - Neuroscience 2020Quote: ... Nonspecific binding was blocked for 1 hr at RT with 5% BLOTTO (Bio-Rad) in Tris-buffered saline with 0.1% Tween (TBST) ...
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bioRxiv - Neuroscience 2020Quote: ... After 1 hour blocking in 5% non-fat milk solution (Bio-Rad 170-6404) at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were blocked for 1 hr using 5% non-fat dry milk (Bio-Rad) and incubated with primary antibody overnight at 4 °C ...
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bioRxiv - Bioengineering 2019Quote: ... Membranes were blocked for 1 h with 5% fat free milk powder (Bio-Rad) in TBS + 0.05% tween-20 (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... Nonspecific binding was blocked for 1 h at room temperature with 5% blotto (Biorad) in Tris-buffered saline with 0.1% Tween (TBST) ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were boiled for 5 min in 1 x Laemmli Sample Buffer (Bio-Rad) containing 5% β-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was diluted 1:5 and SsoFast EvaGreen Supermix was used (BioRad, California, USA). BipA was used as a housekeeping gene.102 Each repeat was performed in a single qPCR plate ...
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bioRxiv - Cell Biology 2021Quote: ... 2-3 μg of total protein were loaded onto 10% SDS-polyacrylamide (29:1 Bio-Rad) gels with 10 μM phostag reagent (FUJIFILM Wako Chemicals ...
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bioRxiv - Molecular Biology 2020Quote: ... Normalised samples (1 – 3 µg) were electrophoresed on 12% Mini-Protean TGX Stain-Free gels (BioRad) or 4-20% Criterion TGX Stain-Free Precast gels (BioRad ...