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Citations for Bio-Rad :
451 - 500 of 7689 citations for 6 oxo 1 phenyl 1 4 5 6 tetrahydro pyridazine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μl cDNA (diluted 1:5) using a CFX384 real-time system qRT-PCR machine (BioRad). Primers were designed using Benchling and purchased from Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were then blocked for 1 h at 25 °C with 5% non-fat dry milk (BioRad) in TBST (50 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 h at room temperature in 5% skim milk (Catalog #170-6404, Bio-Rad). Primary antibody incubations were performed overnight at 4°C with antibodies diluted in TBS/0.1% Tween-20/5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted 1:1000 in PBS-T containing 5% blotting-grade blocker (Bio-Rad, #1706404). Membranes were incubated overnight (≈16 h) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Synthesized cDNA was diluted 1:10 and 5 µl mixed with SsoAdvanced Universal Sybr Green supermix (Biorad). qPCR was performed using the Biorad CFX96 PCR machine ...
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bioRxiv - Biophysics 2021Quote: ... and 3.6 μL ddH2O in a 10 μL total reaction volume using Bio-Rad CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA). The 2-ΔΔCt method was used for the quantitative analysis ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was extracted from several colonies of a 6-week-old subculture on Coletsos culture medium using the InstaGene matrix following the manufacturer’s instructions (Bio-Rad, Marnes-la-Coquette, France). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting transcripts were treated with DNase for 20 min at room temperature and then isolated using Micro Bio-Spin™ 6 Columns (BioRad, Cat.# 732-6221). 0.75 µg of RNA transcripts were mixed with 2X formamide buffer (95% deionized formamide ...
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bioRxiv - Biochemistry 2020Quote: ... the SL2-6 construct was subsequently size purified using a denaturing polyacrylamide gel (7M Urea, 1x TBE, hand-poured in Bio-Rad Criterion midi cassettes), loaded in 80% formamide ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4970) overnight at 4 °C and the HRP-conjugated secondary antibodies (BIO-RAD, Cat. No. 1706515, 1:1000) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... 25 µg (1 µg/µL concentration) of plasmid DNA was added to electroporation 4 mm cuvettes (Bio-Rad) first and then cells added to the cuvettes ...
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bioRxiv - Immunology 2022Quote: ... supernatants were diluted at 1:4 and measured by Bio-Plex Pro mouse IFN-γ immunoassay (Bio-Rad) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Immunostaining was performed overnight at 4° C using primary antibodies for PTH (1:400, Bio-Rad, Hercules, CA), Cleaved caspase 3 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then incubated overnight at 4°C with primary antibodies CD68 (rat monoclonal, 1:400; Biorad, MCA1957), Iba1 (rabbit polyclonal ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated on 12% SDS-polyacrylamide (29:1) or 4-15% precast polyacrylamide mini-gels (Bio-Rad) and electrotransferred on nitrocellulose membranes ...
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bioRxiv - Biochemistry 2023Quote: Intracellular and soluble-secreted proteins were Deoxycholate/Trichloroacetic acid– precipitated and separated by SDS-PAGE on gradient 4-20% PROTEAN TGX Stain-Free Gels (Bio-rad) and proteins were transferred to polyvinylidene difluoride membranes within Trans-Blot Turbo Transfer Packs (Bio-rad ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated 3 h with a solution containing a secondary goat-HRP anti rabbit (1:10 000) (Biorad) (milk 0.5 % TBST) ...
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bioRxiv - Neuroscience 2023Quote: ... Free floating sections were blocked with 3% donkey serum and incubated overnight with rat anti-CD68 (1:200, Bio-Rad). Aβ deposition and CD68 staining were both developed with a Vectastain ABC kit and DAB reaction ...
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bioRxiv - Biochemistry 2023Quote: For VP protein analysis 3×109-1×1010 vg of purified AAV were mixed with 12.5 µl 4x Laemmli Sample Buffer (Bio-Rad, supplemented with 10% 2-mercaptoethanol ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3 μg of protein was separated on 4% to 20% precast polyacrylamide gels (Bio-Rad, cat # 4561095) for detection of histone H3 andH3K27me3 ...
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bioRxiv - Molecular Biology 2023Quote: 4–8 μl of samples and 3 μl of Precision Plus Protein Dual Color Standard (1610374, Bio-Rad) were loaded into 4–12% Bis-Tris Bolt gels (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were blocked overnight at 4°C in PBS with 3% BSA and 10% donkey serum (Bio-Rad), stained with DAPI (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: 5 µL aliquots of the CRISPR complex solution were buffer exchanged into 100 mM ammonium acetate using Biospin P-6 gel columns (Bio-Rad Laboratories Inc., Hercules, CA) prior to native mass spectrometry ...
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bioRxiv - Biophysics 2021Quote: ... The cleaved sample was reloaded on the same column and the flowthrough was collected and applied to a Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges (Bio-rad, Hercules, CA) for desalting ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μg of total proteins were resolved on 10% polyacrylamide gels (except for 6% gels used for PERK detection) and blotted onto PVDF membranes (#1620177, Bio-Rad Laboratories, Hercules, CA, USA). The membranes were blocked with 5% not-fat dry milk or bovine serum albumin (#A7906 ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from the isolates using the InstaGeneTM Matrix comprising 6% (w/v) Chelex resin for PCR-ready DNA purification (Bio-Rad #7326030; California, United States). DNA quality was checked by measuring the A260/280 ratio using NanoDrop ...
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bioRxiv - Cell Biology 2020Quote: ... TBST (50 mM Tris-HCl pH 7.4, 1% Triton X-100) with 5% non-fat milk (Bio-Rad) was used for blocking ...
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bioRxiv - Genetics 2021Quote: ... Samples were then diluted 1:5 and placed into a 96 well plate with Supermix (no dUTPs, BioRad) and the required target locus primers and MGB-NFQ probes (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized with 1-5 μg of RNA using iScript cDNA synthesis kit (Bio-Rad). mRNA levels were measured using qRT-PCR with SYBR Green Master Mix (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... Immunohistochemistry was performed on paraffin sections (5 μm) using antibody F4-80 (Bio-rad, France; diluted 1:100). Sections were incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... 5 min) and incubated for 1 h at room temperature with goat anti-rabbit IgG (H+L) (BioRad). Membranes were washed and signal was detected using the ChemiDoc XRS+ (Biorad ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 h at rtp with 5% (w/v) non-fat milk block (Bio-Rad) in 1x TBS (in mM ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked 1 hour in skim milk 5% TBS-Tween buffer (TBST, Bio-Rad, 0.05% Tween 20), and incubated overnight with the primary antibodies diluted in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 h at room temperature with 5% non-fat dry milk (Bio-Rad Laboratories) in Tris-buffered saline (TBS ...
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bioRxiv - Molecular Biology 2019Quote: ... Either regular (Figure 4 and 5) or stain-free (Figure 8 and 9, Bio-Rad #1610182) 10 % acrylamide gels were used ...
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bioRxiv - Cell Biology 2020Quote: ... then heated at 90°C for 5 min before loading onto 4-20% gel (Bio-Rad). Proteins were separated using running buffer (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... boiled for 5 minutes and resolved on 4 to 20% TGX mini protean gels (Bio-Rad). The proteins were transferred to PVDF membranes using BioRad Trans-Blot semi-dry transfer ...
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bioRxiv - Bioengineering 2019Quote: ... for 5 min at 95 °C and loaded into a 4– 15% polyacrylamide gel (Bio-Rad). Proteins were transferred to a 0.45 µm PVDF membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... 5 μg of sample were loaded on a 4-to-20% SDS polyacrylamide gel (Bio-Rad). Membranes were blocked for 3 hours in 5% milk in TBS (50 mM Trizma base and 150 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... at 100 V for 1 hr in 4°C pre-chilled 1X Tris-Glycine buffer (Bio-Rad 161-0734). Membranes were blocked for 1 hr at room temperature in blocking buffer (PBS (Gibco 14190-144) ...
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bioRxiv - Biochemistry 2020Quote: ... The reconstitution sample was nutated for 1 h at 4°C before addition of 0.2 g/mL SM2-BioBeads (BioRad), and the reconstitution sample was further nutated overnight at 4°C before removal of the biobeads ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 mg of proteins were diluted in ChIP buffer supplemented with 0.01% SDS and precleared 1 h at 4 °C with 50 μl of protein A agarose beads (BioRad) and 100 μg BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed 4 times with TBST and incubated with secondary antibody (1:2000, HRP-conjugated anti rabbit, BioRad) for 1hr at room temperature then washed 4 times with TBST ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was incubated at 4°C for 1 h with Bio-Beads™ SM-2 resin (Bio-Rad) equilibrated with 100 mM Tris-HCl ...
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bioRxiv - Bioengineering 2021Quote: ... A sample containing 1-20µg of protein was loaded into a 4-15% precast gel (Bio-Rad Mini-ProTEAN) and run at 60V for 20min followed by 160V for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Biochemistry 2023Quote: ... CCT was immunoprecipitated from the lysate by adding 4 µg of a CCT5 antibody (BioRAD MCA2178, 1:100 IP) and incubating for 30 minutes at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Gr-1 (1:500, MCA2387, Bio-Rad), Fluorescein labeled DBA-lectin (1:500 ...
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bioRxiv - Immunology 2020Quote: ... IL-6 and IL-17 with housekeeping gene β-actin mRNA by qPCR using the iQ™ SYBR® Green Supermix (Bio-Rad, Hercules, CA, USA). Forward and reverse primers and PCR conditions for RT-PCR are listed in Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were incubated at 30°C for 3-5 days and photographs were taken by Chemi Doc (BioRad).