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Citations for Bio-Rad :
251 - 300 of 424 citations for LS Tetrasaccharide a LSTa Sialyl lacto N tetraose a >90% NMR Sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... the entire samples were applied to Hybond-N using a Bio-Dot® Microfiltration Apparatus (Bio-Rad Laboratories, Inc.). After UV crosslinking ...
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bioRxiv - Cell Biology 2021Quote: ... The N-ter (pI 5.5) and C-ter (pI 9.9) peptides were conjugated to Affi-Gel-10 (Bio-rad) agarose beads for affinity-purification ...
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bioRxiv - Bioengineering 2024Quote: ... Structures were also evaluated by TEM to confirm structural stability following gel purifification using Freeze ’N Squeeze (Bio-Rad) gel extraction columns.
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bioRxiv - Neuroscience 2024Quote: ... Protein levels were visualized using ECL-based autoradiography film system (Super RX-N, Fujifilm) or ChemiDocTM Imaging system (BioRad) and analyzed using Gel Analyzer plugin from ImageJ (Fiji) ...
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bioRxiv - Biophysics 2024Quote: ... the excised band was placed in a Freeze ‘N Squeeze™ DNA Gel Extraction Spin Column (BioRad, 732-6165) and centrifuged for 3 min at 12,000 rcf at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified by preparative agarose gel electrophoresis (Quantum Prep Freeze ‘N Squeeze spin column, Bio-Rad) and magnetic beads (Agencourt AMPure XP ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of purified proteins was determined using the Bradford assay and purity was verified by electrophoresis in sodium dodecyl sulfate (SDS)-polyacrylamide gel which was subsequently stained using Bio-Safe Coomassie G 250 (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... twice in 200 μl nuclease free water and once in 100μl 1x SSC (150 mM NaCl, 15mM sodium citrate, pH 7.0, BIO-RAD Laboratories, Inc.) and finally resuspended in 50 μl 1X SSC ...
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bioRxiv - Biochemistry 2020Quote: Cell lysates were heated at 95°C for 5 min in Laemmli Sample Buffer and then subjected to sodium-dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE; (52)) followed by electrophoretic transfer to 0.45 μm nitrocellulose membranes (Bio-Rad; 1620115). Membranes were blocked in PBS-0.05% Tween20 (PBST ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amount of protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Criterion TGX Stain-Free Precast Gels (Bio-Rad) and transferred onto Immobilon-P membranes (MilliporeSigma) ...
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bioRxiv - Microbiology 2020Quote: ... or the eluates were separated on a 4-15% gradient sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pre-casted gel (Bio-Rad). For Mn2+-Phos-tag SDS-PAGE gel ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were collected then Gag was immunoprecipitated using an α-RSV antibody and were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and quantified using a PhosphorImager (Bio-Rad). After 35S-Met/Cys labeling for 2.5-hours ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated on a polyacrylamide gel with 10% of sodium dodecyl sulfate(SDS) (Mini Protean III; Bio-Rad, Richmond, CA), and they were transferred for 2 hours at 48°C to nitrocellulose membranes of 0.45 μm ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were resolved by SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) using 4-20% precast gel (Biorad, Hercules, CA). Proteins were transferred by semi-dry transfer onto a PVDF (polyvinylidene fluoride ...
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bioRxiv - Microbiology 2021Quote: ... Total proteins (20 μg equivalent) were then separated by 10% or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; Bio-Rad), then transferred by using 0.22-μm polyvinylidene fluoride (PVDF ...
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bioRxiv - Bioengineering 2021Quote: ... Acrylamide/bis solution (30%) 29:1 and natural polypeptide sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) standard were purchased from Bio-Rad. Macrosep and Microsep Advance centrifugal devices 3 kDa MWCO and 0.2 μm syringe filters were purchased from Pall Corporation ...
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bioRxiv - Microbiology 2021Quote: ... or wet transferred using 0.5 M sodium phosphate transfer buffer (at a constant 1 Amp for an hour using a Bio-Rad apparatus). Membranes were blocked in 5% milk in PBS-T and probed with primary antibody (overnight at 4°C ...
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bioRxiv - Pathology 2020Quote: ... 5 microliters of urine were run on 4-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and stained with Coomassie Blue Stain Solution (Bio-Rad) to detect proteinuria ...
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bioRxiv - Molecular Biology 2021Quote: ... Total proteins extracted with 1 X SDS were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (1620112, Bio-Rad). The membranes were blocked in 5% BSA for 1 hour at RT and probed with primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... 5 µL of samples were loaded into a precast 4-20% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) alongside size standards (Precision Protein Plus ...
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bioRxiv - Immunology 2021Quote: ... The proteins were fractionated on a 10% sodium dodecyl sulfate–polyacrylamide gel and transferred to polyvinylidene difluoride (PVDF) membrane using a semi dry Transfer Cell (Bio-Rad), which was run at 18 V for 1h ...
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bioRxiv - Bioengineering 2021Quote: ... Acrylamide/bis solution (30%) 29:1 and natural polypeptide sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) standard were purchased from Bio-Rad. Agarose was purchased from Sigma-Aldrich ...
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bioRxiv - Pathology 2021Quote: ... and equal volumes of each suspension were loaded into two sodium dodecyl sulphate-polyacrylamide gels (SDS-PAGE; 4-20% Mini-PROTEAN TGX Protein Gels, Bio-Rad) and run at 150 V for one hour ...
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bioRxiv - Genetics 2021Quote: ... SDS-PAGE (Sodium Dodecyl Sulphate–PolyAcrylamide Gel Electrophoresis) was run for 30 minutes at 120V in Tris/Glycine/SDS buffer (BioRad, #1610732). Protein was then transferred onto PVDF membrane using Bio-Rad TransBlot Turbo system using the low molecular weight protocol ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from lysates were separated by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE; Bio-Rad, Munich) and subsequently transferred to a nitrocellulose membrane by electroblotting ...
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bioRxiv - Microbiology 2021Quote: ... boiled for 5 min at 95 °C and separated by discontinuous sodium dodecyl sulfate (SDS) 4-15% polyacrylamide gel electrophoresis (SDS-PAGE precast gels, Bio-Rad). The SDS-PAGE gels were stained in Coomassie Blue solution (Bio-Rad).
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bioRxiv - Immunology 2021Quote: Purified proteins were analysed for purity determination via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 4-20% gradient gel (BioRad – 4568094), with or without the presence of 2-mercaptoethanol as reducing agent.
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bioRxiv - Cell Biology 2021Quote: ... 30 µg of protein sample was loaded onto a 4 to 15% gradient sodium dodecyl sulfate (SDS)-polyacrylamid gel (Bio-Rad), followed by protein transfer to a polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes (BioRad, California, USA), which were blocked with 5% skim milk for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: The purified baculoviruses were electrophoresed on 10% sodium dodecyl sulfate-polyacrylamide gel (BIOTOOLS) and transferred to a low-fluorescence polyvinylidene fluoride (PVDF) membrane (Bio-Rad) through Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Lysed samples were separated on a SDS (sodium dodecyl sulfate) polyacrylamide gel and blotted onto a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Samples were boiled for 5 min at 95°C before separation on a gradient 4 to 20% sodium dodecyl sulfate (SDS)-polyacrylamide gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semidry transfer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysates were separated by Bis-Tris sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4–20% precast polyacrylamide gel (BioRad, #4561096). The proteins were transferred to low fluorescence polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were loaded for electrophoresis in 5% or 15% polyacrylamide gels containing sodium dodecyl sulfate (SDS‒PAGE) and transferred onto nitrocellulose membranes using the Trans-Blot Turbo system (Bio-Rad). The membranes were incubated with the primary antibodies listed in Supplemental Table S1 at 4 °C overnight after blocking with Blocking One reagent (Nacalai Tesque) ...
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bioRxiv - Genetics 2024Quote: ... Forty micrograms of whole-cell lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Bio-Rad). The membranes were blocked with 5% nonfat dry milk in Tris-buffered saline with 0.05% Tween 20 (TBST ...
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bioRxiv - Cancer Biology 2022Quote: 40 µg of proteins were separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 10% acrylamide gel (BIO-RAD) and transferred to nitrocellulose membrane by semi-dry blotting using the Trans-Blot Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from lysed cells or immunoprecipitates were denatured and loaded on sodium dodecyl sulfate polyacrylamide gels and then transferred to polyvinylidene difluoride membranes (Bio-Rad). After blocking with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and wet-transferred overnight at 150 mA to Hybond nitrocellulose membranes (Bio-Rad) as described (125 ...
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bioRxiv - Systems Biology 2023Quote: ... by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany, 1658005EDU). Proteins were transferred to a nitrocellulose membrane (Carl Roth ...
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bioRxiv - Genomics 2023Quote: ... from cell preparations were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a PVDF membrane (Bio-Rad) using a Trans-Blot Turbo™ transfer system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein lysates were run on 10% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and transferred to a nitrocellulose membrane (Bio-Rad) using a wet tank blotting system ...
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bioRxiv - Biochemistry 2023Quote: ... were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using 4-20% Mini-Protean TGX gradient gels (4561094, Bio-RAD) and stained with Coomassie Blue ...
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bioRxiv - Physiology 2023Quote: ... Lysates were separated by sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) and transferred onto nitrocellulose membranes (Bio-Rad Laboratories, US) using a semidry technique (semidry blotter ...
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bioRxiv - Cancer Biology 2023Quote: ... Whole-cell lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene fluoride membrane (Bio-Rad). The blots were incubated with anti-CCTα (D18B6 ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were electrophoresed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) precast 4-20 % Mini-PROTEAN TGX gel (Bio-Rad) to separate the proteins.
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bioRxiv - Systems Biology 2023Quote: ... by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany, 1658005EDU).
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bioRxiv - Pathology 2023Quote: ... 5.0% sodium hypochlorite) with one drop of Tween 20 added to every 50 ml of solution (Bio-Rad Laboratories, Hercules, CA) for 10 s to disinfest their surfaces ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Proteins in cytosolic or nuclear extracts from mouse livers were resolved in sodium dodecyl sulphate-polyacrylamide gel electrophoresis using the mini-PROTEAN TGX Stain-Free Precast Gels from Bio-Rad. Primary antibodies were revealed with HRP-conjugated secondary antibodies (anti-rabbit IgG #7074 ...
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bioRxiv - Biochemistry 2023Quote: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed as previously described.44 In a vertical gel electrophoresis device (Bio-Rad, USA), 4% concentrated gel and 8% separated gel were used ...