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Citations for Bio-Rad :
101 - 150 of 424 citations for LS Tetrasaccharide a LSTa Sialyl lacto N tetraose a >90% NMR Sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 0.5% sodium deoxycholate) and 7.5uL of 4X Laemmli buffer (1610747; BioRad, Hercules, CA) and run on 10-20% Criterion Tris-HCl gels (3450042 ...
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bioRxiv - Neuroscience 2024Quote: ... Urea and Sodium dodecyl sulfate (SDS) were purchased from Bio-Rad (Hercules, CA). Acetonitrile and water for nano-LC−MS/MS were purchased from J ...
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bioRxiv - Immunology 2024Quote: ... Adherent cells were lysed in sodium dodecyl sulfate (SDS) sample lysis buffer (BioRad). Proteins were separated by gel electrophoresis on a 10% Tris-Glycine gel (BioRad) ...
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bioRxiv - Molecular Biology 2022Quote: ... separated on a 4-15% sodium dodecyl sulfate/ polyacrylamide gel electrophoresis gel (BioRad) and transferred to a nitrocellulose membrane (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... final concentrations of 2X SsoADV Universal SYBR Green Supermix (BIORAD, n° 1725270) and 0.04μM of each primer ...
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bioRxiv - Biophysics 2023Quote: ... and samples were extracted with Freeze N’ Squeeze spin columns (Bio-Rad) by centrifugation at 1,000 g for 60 minutes at 4 ºC ...
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bioRxiv - Microbiology 2024Quote: ... blocked with Intercept™ (TBS) buffer (Bio-Rad, P/N: 927-70001) or 5% skim milk for 1 hour at room temperature and incubated with primary antibodies at 4°C overnight ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.5 was heated from 15 ºC to 90 ºC with 0.5 ºC increment every 30 seconds on an iCycle iQ5 Real Time Detection System (Bio-Rad, Hercules, CA). The normalized fluorescence data was plotted against temperature (53).
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bioRxiv - Biophysics 2022Quote: ... Electrophoresis was carried out for 1 hour at 90 V using an electrophoresis system (mini–DNA SUB CELL, Bio-Rad, Mississauga, Canada). The DNA fragments were visualized in a Gel Doc XR+ Gel Documentation System (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was mixed on an orbital shaker for 60 – 90 minutes at 4 °C and then transferred to a glass column (BIO-RAD EconoColumn). Non-His-Tagged proteins were eluted with lysis buffer until the absorbance at 280 nm matched background levels utilizing 50 – 100 mL of lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Protein extracts were subjected to SDS-PAGE for 90 min at 130 V at room temperature on 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad #4568046). Samples were transferred to nitrocellulose membranes using a BioRad Trans-Blot Turbo system (“standard sd” protocol) ...
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bioRxiv - Bioengineering 2020Quote: ... and annealed from 90°C to 20°C at 1 °C min-1 in a T100™ Thermal Cycler (Bio-Rad, USA). Following construction ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were incubated at room temperature with rotation for 90 minutes and stopped by desalting using Micro Bio-Spin Columns with Bio-Gel P-30 (Bio-Rad Laboratories). Reacted sequences were isolated by addition of one sample volume of Streptavidin MagneSphere paramagnetic beads (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were incubated at room temperature with rotation for 90 minutes and stopped by desalting using Micro Bio-Spin Columns with Bio-Gel P-30 (Bio-Rad Laboratories). Reacted sequences were isolated with 100 μL Streptavidin MagneSphere paramagnetic beads (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were incubated at room temperature for 90 minutes with rotation and stopped by desalting using Micro Bio-Spin Columns with Bio-Gel P-30 (Bio-Rad Laboratories). 95 nmol of streptavidin (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
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bioRxiv - Cell Biology 2024Quote: ... Migration was performed for 10 minutes at 80 mV followed by 90 minutes at 120 mV until the 30 kDa molecular weight marker (Bio-Rad, 1610374) reached the bottom of the gel ...
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bioRxiv - Biochemistry 2024Quote: ... and electrophoresis was run under a N2/CO2 (90:10 %) atmosphere with a constant 40 mA current (PowerPac™ Basic Power Supply, Bio-Rad). After electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... These proteins were then subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad) and transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: Sodium-Dodecyl-Sulphate (SDS) Polyacrylamide Gel Electrophoresis was done in precast gels (Bio-Rad TGX Mini-Protean 4-15% gels with Dual Colour Standard ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Proteins (10-50 µg) were loaded onto 10% sodium dodecyl sulfate polyacrylamide gel (BioRad), separated by electrophoresis (SDS-PAGE) ...
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bioRxiv - Immunology 2024Quote: ... Whole-cell lysates (RIPA buffer) separated on 10% sodium dodecyl sulfate-polyacrylamide (Bio-Rad) gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... crushed and structures were purified with Freeze ‘N Squeeze spin columns (Bio-Rad) for 5 min at 1,000×g at 4 °C.
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bioRxiv - Biophysics 2020Quote: ... crushed and spun through a Freeze N’ Squeeze column (BioRad Sciences, 732-6165) for 3 min at 13,000g at 4°C ...
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bioRxiv - Immunology 2024Quote: ... filtered through Freeze ‘N Squeeze DNA Gel Extraction Spin Columns (Bio-Rad; #7326165) and purified via ethanol precipitation ...
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bioRxiv - Biophysics 2024Quote: ... Freeze ‘N Squeeze columns (catalog no. 732-6165) were obtained from Bio-Rad. Cy3b-maleimide (catalog no ...
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bioRxiv - Synthetic Biology 2024Quote: ... filtering through Freeze ‘N Squeeze DNA Gel Extraction Spin Columns (Bio-Rad; #7326165) and then finally performing ethanol precipitation ...
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bioRxiv - Synthetic Biology 2024Quote: ... filtering through Freeze ‘N Squeeze DNA Gel Extraction Spin Columns (Bio-Rad; #7326165) and then using ethanol precipitation ...
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bioRxiv - Cancer Biology 2024Quote: ... WHO°3 n=3) using the Bio-Plex Cell Lysis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The protein was eluted the next day and was buffer exchanged into a low salt buffer (75mM HEPES, pH 7.6, 100mM KCl, 10% glycerol, 2mM DTT) using a 10-DG column (Bio-rad) for further purification ...
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bioRxiv - Biochemistry 2024Quote: ... Purified GroEL and GroES were first buffer-exchanged into RNC low-salt buffer using Micro Bio-Spin 6 Columns (Biorad) to remove any traces of Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE (90 min at 150V) using 4-20% Criterion™ TGX Stain-Free™ gel (Bio-Rad, 5678095) and electro-blotted onto nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... placed on a heat block (+90 °C) for 10 min and sonicated before separation by SDS-PAGE (4-20% Mini-PROTEAN TGX Gels, Bio-Rad, 456-1096). Next ...
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 90°C and loaded on a 4–20% (w/v) Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were separated by electrophoresis and blotted onto a membrane with trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... samples of 5-10 μg protein were reduced with 50 mM dithiothreitol (DTT) for 15 minutes at 90°C and mixed with 4× sample loading buffer (Bio-Rad, Munich, Germany). Protein separation was carried out via SDS-PAGE in 12% polyacrylamide gels (Mini-PROTEAN® TGX™ Precast Gels ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated briefly at 90°C and then transferred to a Trans-Blot Turbo mini nitrocellulose membrane using Trans-Blot Turbo Transfer system (Bio-Rad, Hercules, CA). The membrane was incubated overnight in antibody buffer (50mM tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... and the sample incubated at 90°C for 5 min and resolved by electrophoresis through a 4–20% gradient polyacrylamide/SDS gel (Bio-Rad Laboratories, 5671085). Next ...
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bioRxiv - Bioengineering 2023Quote: ... were irradiated using the pre-programmed sterilisation setting (maximum wavelength of 253.7 nm, 90 seconds exposure) on the GS Gene Linker UV chamber (Bio-Rad laboratories, California, USA). Samples placed in petri dishes received three repeats of irradiation for top and bottom surfaces of the dish ...
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bioRxiv - Cell Biology 2024Quote: ... samples were placed on a heat block (+90°C) for 10 minutes and separated by SDS–polyacrylamide gel electrophoresis (4–20% Mini-PROTEAN TGX gels, Bio-Rad, 456-1096). Proteins were transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... dissolved in a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer (Bio-Rad) and separated on 15 % SDS-PAGE gels following the Laemmli method (Laemmli ...
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bioRxiv - Neuroscience 2022Quote: ... A 12% sodium dodecyl sulfate-polyacrylamide gel was prepared according to manufacturer’s protocol (BIO-RAD). For the analysis 32 μl of the production supernatant was mixed with 8 μl 5xLaemmli buffer containing ß-Mercaptoethanol and incubated for 10 min at 95°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... miR-146a-loaded MLNPs were lysed with 0.5% sodium dodecyl sulfate (SDS) (BioRad, Hercules, CA), and loaded onto 2% agarose (Fisher BioReagents ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated in 4–15% sodium dodecyl sulfate/polyacrylamide gel electrophoresis gel (Bio-Rad) and transferred to nitrocellulose membranes ...
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bioRxiv - Bioengineering 2024Quote: ... in 0.1M sodium phosphate buffer pH 7.3 previously treated with Chelex 100 chelating resin (BioRad), and reacted at 4°C for 18 hr ...
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bioRxiv - Neuroscience 2022Quote: ... proteins were transferred onto a 0.45 μm nitrocellulose membrane (Bio-Rad, catalog n° 1620115) for 1 hour at RT at 100V ...
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bioRxiv - Bioengineering 2021Quote: ... Samples for AFM imaging were purified by using the Freeze ‘N Squeeze kit (BioRad) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: ... Images were taken by ChemiDoc™ MP Imaging System (Bio-Rad, Catalog N° #12003154).
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bioRxiv - Bioengineering 2024Quote: ... The samples were purified for TEM imaging using the Freeze ’N Squeeze (Bio-Rad) gel extraction column and imaging samples were prepared as previously described.
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bioRxiv - Bioengineering 2023Quote: ... Purification of DNA origami structures was performed by gel extraction (Freeze ‘N Squeeze, BioRad) or PEG precipitation (46) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 ul of suspended cells were then added to a 10ul solution of 0.4% trypan blue in a buffered isotonic salt solution (Bio-Rad, #1450021), pH 7.3 ...