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Citations for Bio-Rad :
51 - 100 of 424 citations for LS Tetrasaccharide a LSTa Sialyl lacto N tetraose a >90% NMR Sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 80-90% of each sample was separated by pre-cast 4-20% SDS-PAGE (Bio-Rad). “SimplyBlue SafeStain” (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from cells transfected with pCMVΔR8.91 using TRIzol LS reagent and PCR amplified with primers DR22 and DR23 using IQ SYBR Green Supermix (BioRad, 1708882). Total RNA from 0.33×106 cells was used for electrophoretic analysis of rRNA integrity with an Agilent Technologies 4200 TapeStation ...
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bioRxiv - Microbiology 2024Quote: ... HKU1 NTD and pA-LS NP protein expression was analyzed using SDS-PAGE and subsequent Western blot on PVDF membrane (Biorad) using StrepMAB-Classic HRP 1:3000 (2-1509-001 ...
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bioRxiv - Cell Biology 2020Quote: ... which then heated at 90°C for 5 min before loading onto 4-20% gel (Bio-Rad). Proteins were separated using running buffer (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... for 90 min at 35V in 0.5x TBE using the TransBlot Mini transfer apparatus (Bio-Rad, USA) and UV-crosslinking at 1200 kDj/cm2 ...
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bioRxiv - Microbiology 2021Quote: ... for 90 min at 35V in 0.5x TBE using the TransBlot Mini transfer module (Bio-Rad, USA) and UV-crosslinking at 1200 kDj/cm2 using UV Stratalinker 1800 (Stratagene ...
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bioRxiv - Biochemistry 2021Quote: ... Approximately 90-100 μg of each sample was loaded on 4-20% precast gradient gel (Bio-Rad) and resolved proteins were transferred to a PVDF membrane ...
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bioRxiv - Immunology 2024Quote: ... followed by 120 V for 90 minutes in a vertical electrophoresis cell (Bio-Rad Laboratories, Feldkirchen, Germany). Proteins were transferred to nitrocellulose membranes at 90 V for 90-100 minutes by a Mini Trans- Blot cell (Bio-Rad Laboratories ...
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bioRxiv - Systems Biology 2024Quote: ... buffer + 2% (v/v) Sodium dodecyl sulfate (SDS, Bio-Rad). Proteins were denatured at 95 °C and 600 rpm for 10 min before adding a final concentration of 2% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... Amount of formazan salt was measured in a microplate reader (Bio-Rad Serial no. 19901) at OD of 590 nm.
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bioRxiv - Molecular Biology 2020Quote: ... was carried out for 90 min at 25 W in a Mini Trans-Blot Electrophoretic Transfer Cell (BioRad). Membranes were blocked for 3 h with 5% w/v nonfat dry milk in 0.05% Tween 20 in PBS (PBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal total protein concentration were heated at 90°C in 1 × loading buffer (Bio-Rad 1610747) for ten minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were run for 90 min at 150 V in TGS buffer on a MiniProtean setup (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... Excessive salt and residual NTPs were removed by using P30 column (Bio-Rad, Cat# 732–6250), followed by treatment with DNase I (Promega Cat# M6101 ...
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bioRxiv - Microbiology 2022Quote: ... anti-Metapneumovirus N mouse monoclonal (1:25) (MCA4674, Bio-Rad), or anti-Influenza A nucleoprotein (NP ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (BioRad). Following gel electrophoresis ...
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bioRxiv - Immunology 2024Quote: ... RPMI 1640 medium (Bio-Rad, 11-100-1K, without sodium bicarbonate) was supplemented with 2 mM glutamine (Satorius ...
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bioRxiv - Neuroscience 2020Quote: ... was dissolved in 100 μl dichloromethane and applied to 90 mg of 1.3 μm tungsten microcarrier particles (Bio-Rad) spread out evenly on a glass slide ...
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bioRxiv - Cell Biology 2024Quote: ... SARS-CoV2-PP were prepared by labelling with 0.4µM DiIC18(5) solid (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt, or DID) (Thermo) with Biospin6 (Biorad) to remove residual dyes ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% (w/v) of sodium dodecyl sulfate (SDS, Biorad, catalog #: 1610302). Protein ladder was added (Biorad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sodium dodecyl sulfate (SDS) and PVDF membrane were procured from Bio-Rad. The protein ladder was purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... was purchased from G-Biosciences and Freeze ‘N Squeeze DNA gel extraction columns by Bio-rad, Inc ...
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bioRxiv - Biophysics 2022Quote: ... which were subsequently used with Freeze ‘N Squeeze spin columns (BioRad).
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bioRxiv - Microbiology 2022Quote: ... the plates were sterilized using the sterilization setting (90 s) in a GS Gene Linker UV Chamber (Bio-Rad, Inc.). 500 µl of overnight cultures of S ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed and then precipitated in 0.1 M glycine pH 3.5 and neutralized with 0.5 M Tris?Cl and 1.5 M NaCl pH 7.4 before SDS-PAGE electrophoresis (8%, at 100 V for 90 min) followed by transfer to a PVDF membrane (BioRad). Membranes were washed in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2021Quote: ... in pre-chilled 0.5xTBE buffer for 90 min at 50 V using Mini Trans-Blot electrophoretic transfer cell (Bio-Rad). Transferred RNA was UV-crosslinked to membrane and hybridized with [γ- 32P] 5’-end labeled RFP-spacer specific probe (RFP_crRNA_probe ...
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bioRxiv - Microbiology 2020Quote: ... The samples were heated at 90°C for 10 minutes and loaded onto a 4-20% precast polyacrylamide gel (BioRad). The gel was stained with SimplyBlue SafeStain (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were run on SDS-PAGE gel for 90-120 minutes and then blotted on nitrocellulose membrane using semi-dry blotting machine (Biorad). Two antibodies were used ...
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bioRxiv - Neuroscience 2022Quote: ... the gels were wet transferred at 400 mA for 90 min to methanol-activated 0.2 µm pore size PVDF membrane (Bio-Rad). Blots were blocked in 5% nonfat milk in Tris-buffered saline (0.5 mM Tris-HCl [pH 7.5] and 1.5 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled 5min at 90°C and 10µl was loaded on a 4-20% gradient SDS-PAGE gel (Biorad #4561096). Gel was stained with Coomassie brilliant blue R.
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bioRxiv - Biochemistry 2024Quote: ... at a 20-90°C gradient (increment: 0.3 °C, hold for 5 s before read) on a Real-time PCR system (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was resolved by electrophoresis on a 6% non-denaturing polyacrylamide gel in TBE (100 mM Tris, 90 mM boric acid, 1 mM EDTA) buffer and visualised by ChemiDoc (BioRad) using SYBR Gold stain or imaging in the 6FAM ...
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bioRxiv - Molecular Biology 2024Quote: ... assembled with membranes pre-equilibrated with binding buffer without salts (top: nitrocellulose membrane (0.45 μm pore size; BioRad); bottom ...
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bioRxiv - Bioengineering 2022Quote: ... The α-FN-EDA Fab was verified as >90% pure by SDS-PAGE (performed on 4-20% gradient gels (Bio-Rad)).
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer B + 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer D ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer C + 400 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Proteins that bound to PI(3)P or control beads were eluted by boiling at 90–95 °C for 20 min in 70 μL 4x Laemmli sample buffer (Bio-Rad) and centrifuging at 20,000 g for 15 min ...
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bioRxiv - Genetics 2022Quote: ... The samples were boiled at 65°C for 10 minutes and then loaded on 10% SDS-PAGE gel and ran at 90 volts for 2 hours and transferred to a PDVF membrane via semidry transfer (Bio-Rad) at 13 V for 3 hours ...
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bioRxiv - Microbiology 2022Quote: ... 20% (v/v) methanol) at a constant amperage of 300 mA for 90 min using a Mini Trans-Blot Cell (Bio-Rad). The membranes were incubated in NRA blocking buffer (INNOVAX ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated on ExpressPlus™ PAGE Ready gels 4 - 20% (A2S, M42015) and transferred on the cellulose membrane (300 mA, 90 min) using a BioRad blotting device (BioRad, USA). After overnight blocking in 5% milk in TBST ...
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bioRxiv - Microbiology 2022Quote: ... 14 µL of PCR product were then transformed by electroporation in 90 µL cells (2.5 kV electric pulse for 5 ms, Bio-Rad micropulser). Cells were recovered in SOC outgrowth medium (NEB B9020S ...
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bioRxiv - Biochemistry 2022Quote: ... in denaturing conditions after incubation into XT-sample buffer (Bio Rad) for 5 min at 90 °C with 12% Bis-Tris Criterion XT precast gels (Biorad, USA) with 1x XT-MOPS buffer at fixed current of 25 mA for 1.5-2 h ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by electrophoresis at a constant 130 V/gel for 90 min and transferred to a nitrocellulose membrane on the Trans-Blot Turbo Transfer System (Bio-Rad) at 25 V for 7 min ...
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bioRxiv - Systems Biology 2023Quote: ... Cell pellets were lysed by heating (95°C for 15 minutes) in a reducing buffer consisting of 90% Invitrogen Laemmli buffer (Bio-Rad) and 10% NuPAGE (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... The proteins were transferred onto a 0.45 µm nitrocellulose membrane at 75 V for 90 min using a transfer assembly (BioRad Laboratories Inc.). The membrane was washed with 0.1% tween-20-containing tris buffer saline (T-TBS ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by electrophoresis at a constant 130 V/gel for 90 min and transferred to a nitrocellulose membrane on the Trans-Blot Turbo Transfer System (Bio-Rad) at 25 V for 7 min ...
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bioRxiv - Biochemistry 2023Quote: ... The cell viability (at least 90%) and the cell count before seeding were carried out using the Automated Cell Counter (Biorad, USA).
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bioRxiv - Physiology 2024Quote: ... Proteins were transferred to a methanol-activated PVDF membrane (IPFL85R, sigma) at 300 mA for 90 min at room temperature (1703930, Bio-Rad). The membrane was washed with 1x TBS for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were eluted into sodium dodecyl sulfate (SDS) sample buffer (Bio-Rad) containing 5% 2-mercaptoethanl (Sigma ...