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Citations for Bio-Rad :
251 - 300 of 397 citations for Flumoxef sodium impurity P since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene difluoride membrane (Cat# 1620177, BioRad). Following the protein transfer ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then incubated eMAP hydrogel monomer solution (30% acrylamide [A9099, MilliporeSigma, St. Louis, MO, USA], 10% sodium acrylate [408220, MilliporeSigma], 0.1% bis-acrylamide [161-0142, Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were electrophoresed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis using a 4%–20% gradient polyacrylamide gel (Bio-Rad, 5678093) and transferred to 0.45-μm polyvinylidene difluoride membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... and exchanged into 100 mM sodium phosphate buffer (pH 7.0) supplemented with 100 mM sodium chloride using Econo-Pac 10DG desalting pre-packed gravity-flow columns (Bio-Rad). Proteins used for crystallization were further purified by gel filtration in 50 mM sodium citrate buffer (pH 5.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins used for crystallization were further purified by gel filtration in 50 mM sodium citrate buffer (pH 5.5) and 150 mM sodium chloride using an ENrich SEC 650 size-exclusion chromatography column (Bio-Rad). Purified samples were concentrated using Amicon Ultracel-3K centrifugal filter units (EMD Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... 25 μg protein extracts were separated by sodium dodecyl sulfate (SDS) gel electrophoresis and transferred to a polyvinylidene difluoride (PVDF) membrane (BioRad) overnight ...
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bioRxiv - Biophysics 2022Quote: ... washes and elutes were examined using SDS-PAGE (sodium dodecyl sulfate– polyacrylamide gel electrophoresis) (Gel: BioRad miniPROTEAN TGX AnyKD - 4569036; MW latter: BioRad Precession Plus Protein Standard Unstained – 1610363) ...
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bioRxiv - Genetics 2023Quote: ... The beads were washed four times for 5 min with RIP buffer containing 0.5% sodium deoxycholate and boiled in 1xLaemmli Sample Buffer (Bio-Rad) for 10 min at 100°C ...
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bioRxiv - Cancer Biology 2023Quote: ... separated by sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% of bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... onto 4-15% precast criterion sodium dodecyl sulfate polyacrylamide gel electrophoresis gradient gels and transferred to PVDF per manufacturer’s protocol (Bio-Rad). The protein sizing ladder used was Precision Plus Protein Standards (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 µg of protein was separated on a 4–20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Neuroscience 2024Quote: ... were electrophoresed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) and transferred proteins from gel onto polyvinylidene difluoride fluoride (PVDF) membranes (BioRad). After transformation ...
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bioRxiv - Developmental Biology 2024Quote: Prepared samples were loaded onto a 7.5% (Elp1) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) (Biorad 4561033, 4561024 respectively) and separated by electrophoresis at 70V for approximately 2.5 hours in 1X running buffer (diluted from 10X running buffer (25mM Tris base ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were boiled in SDS loading buffer and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to 0.45 µm nitrocellulose membranes (Bio-Rad). The following primary antibodies were used ...
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bioRxiv - Biochemistry 2021Quote: ... and their respective complexes with Hap40 protein were buffer-exchanged into 150 mM aqueous ammonium acetate (pH=7.5) by using P-6 Bio-Spin gel filtration columns (Bio-rad, Veenendaal, the Netherlands). The protein’s resulting concentration was estimated to be ~2-5 μM before native MS analysis ...
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bioRxiv - Microbiology 2021Quote: ... and phospho-Akt (p-Akt) were quantified in 10 μg of total protein using the Bio-Plex Pro magnetic phosphoprotein detection assay (Bio-Rad, United Kingdom). Phosphoprotein expression was normalized to β-actin and expressed as fold-change relative to the uninfected (UI ...
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bioRxiv - Pathology 2020Quote: ... LC3-II and p-mTOR were separated by electrophoresis and then transferred onto the nitrocellulose membranes (Bio-Rad; Trans-Blot Turbo Transfer System). After several washes with TBST buffer ...
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bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of purified proteins was determined using the Bradford assay and purity was verified by electrophoresis in sodium dodecyl sulfate (SDS)-polyacrylamide gel which was subsequently stained using Bio-Safe Coomassie G 250 (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... twice in 200 μl nuclease free water and once in 100μl 1x SSC (150 mM NaCl, 15mM sodium citrate, pH 7.0, BIO-RAD Laboratories, Inc.) and finally resuspended in 50 μl 1X SSC ...
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bioRxiv - Biochemistry 2020Quote: Cell lysates were heated at 95°C for 5 min in Laemmli Sample Buffer and then subjected to sodium-dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE; (52)) followed by electrophoretic transfer to 0.45 μm nitrocellulose membranes (Bio-Rad; 1620115). Membranes were blocked in PBS-0.05% Tween20 (PBST ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amount of protein was resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Criterion TGX Stain-Free Precast Gels (Bio-Rad) and transferred onto Immobilon-P membranes (MilliporeSigma) ...
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bioRxiv - Microbiology 2020Quote: ... or the eluates were separated on a 4-15% gradient sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pre-casted gel (Bio-Rad). For Mn2+-Phos-tag SDS-PAGE gel ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were collected then Gag was immunoprecipitated using an α-RSV antibody and were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and quantified using a PhosphorImager (Bio-Rad). After 35S-Met/Cys labeling for 2.5-hours ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated on a polyacrylamide gel with 10% of sodium dodecyl sulfate(SDS) (Mini Protean III; Bio-Rad, Richmond, CA), and they were transferred for 2 hours at 48°C to nitrocellulose membranes of 0.45 μm ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were resolved by SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) using 4-20% precast gel (Biorad, Hercules, CA). Proteins were transferred by semi-dry transfer onto a PVDF (polyvinylidene fluoride ...
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bioRxiv - Microbiology 2021Quote: ... Total proteins (20 μg equivalent) were then separated by 10% or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; Bio-Rad), then transferred by using 0.22-μm polyvinylidene fluoride (PVDF ...
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bioRxiv - Bioengineering 2021Quote: ... Acrylamide/bis solution (30%) 29:1 and natural polypeptide sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) standard were purchased from Bio-Rad. Macrosep and Microsep Advance centrifugal devices 3 kDa MWCO and 0.2 μm syringe filters were purchased from Pall Corporation ...
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bioRxiv - Microbiology 2021Quote: ... or wet transferred using 0.5 M sodium phosphate transfer buffer (at a constant 1 Amp for an hour using a Bio-Rad apparatus). Membranes were blocked in 5% milk in PBS-T and probed with primary antibody (overnight at 4°C ...
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bioRxiv - Pathology 2020Quote: ... 5 microliters of urine were run on 4-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and stained with Coomassie Blue Stain Solution (Bio-Rad) to detect proteinuria ...
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bioRxiv - Molecular Biology 2021Quote: ... Total proteins extracted with 1 X SDS were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (1620112, Bio-Rad). The membranes were blocked in 5% BSA for 1 hour at RT and probed with primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... 5 µL of samples were loaded into a precast 4-20% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad) alongside size standards (Precision Protein Plus ...
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bioRxiv - Immunology 2021Quote: ... The proteins were fractionated on a 10% sodium dodecyl sulfate–polyacrylamide gel and transferred to polyvinylidene difluoride (PVDF) membrane using a semi dry Transfer Cell (Bio-Rad), which was run at 18 V for 1h ...
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bioRxiv - Bioengineering 2021Quote: ... Acrylamide/bis solution (30%) 29:1 and natural polypeptide sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) standard were purchased from Bio-Rad. Agarose was purchased from Sigma-Aldrich ...
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bioRxiv - Pathology 2021Quote: ... and equal volumes of each suspension were loaded into two sodium dodecyl sulphate-polyacrylamide gels (SDS-PAGE; 4-20% Mini-PROTEAN TGX Protein Gels, Bio-Rad) and run at 150 V for one hour ...
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bioRxiv - Genetics 2021Quote: ... SDS-PAGE (Sodium Dodecyl Sulphate–PolyAcrylamide Gel Electrophoresis) was run for 30 minutes at 120V in Tris/Glycine/SDS buffer (BioRad, #1610732). Protein was then transferred onto PVDF membrane using Bio-Rad TransBlot Turbo system using the low molecular weight protocol ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from lysates were separated by electrophoresis in a sodium dodecyl sulfate (SDS)-10% polyacrylamide gel (SDS-PAGE; Bio-Rad, Munich) and subsequently transferred to a nitrocellulose membrane by electroblotting ...
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bioRxiv - Microbiology 2021Quote: ... boiled for 5 min at 95 °C and separated by discontinuous sodium dodecyl sulfate (SDS) 4-15% polyacrylamide gel electrophoresis (SDS-PAGE precast gels, Bio-Rad). The SDS-PAGE gels were stained in Coomassie Blue solution (Bio-Rad).
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bioRxiv - Immunology 2021Quote: Purified proteins were analysed for purity determination via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 4-20% gradient gel (BioRad – 4568094), with or without the presence of 2-mercaptoethanol as reducing agent.
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bioRxiv - Cell Biology 2021Quote: ... 30 µg of protein sample was loaded onto a 4 to 15% gradient sodium dodecyl sulfate (SDS)-polyacrylamid gel (Bio-Rad), followed by protein transfer to a polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2022Quote: 40 µg of proteins were separated using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 10% acrylamide gel (BIO-RAD) and transferred to nitrocellulose membrane by semi-dry blotting using the Trans-Blot Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were electrophoresed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) precast 4-20 % Mini-PROTEAN TGX gel (Bio-Rad) to separate the proteins.
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bioRxiv - Cell Biology 2023Quote: All the protein samples were separated by sodium dodecyl sulfate polyacrylamide (SDS)-PAGE and were transferred to nitrocellulose membrane (Bio-Rad laboratories ...
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bioRxiv - Genomics 2023Quote: ... from cell preparations were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a PVDF membrane (Bio-Rad) using a Trans-Blot Turbo™ transfer system (Bio-Rad) ...
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bioRxiv - Systems Biology 2023Quote: ... by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany, 1658005EDU). Proteins were transferred to a nitrocellulose membrane (Carl Roth ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from lysed cells or immunoprecipitates were denatured and loaded on sodium dodecyl sulfate polyacrylamide gels and then transferred to polyvinylidene difluoride membranes (Bio-Rad). After blocking with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and wet-transferred overnight at 150 mA to Hybond nitrocellulose membranes (Bio-Rad) as described (125 ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein lysates were run on 10% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and transferred to a nitrocellulose membrane (Bio-Rad) using a wet tank blotting system ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysates were separated by Bis-Tris sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 4–20% precast polyacrylamide gel (BioRad, #4561096). The proteins were transferred to low fluorescence polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were loaded for electrophoresis in 5% or 15% polyacrylamide gels containing sodium dodecyl sulfate (SDS‒PAGE) and transferred onto nitrocellulose membranes using the Trans-Blot Turbo system (Bio-Rad). The membranes were incubated with the primary antibodies listed in Supplemental Table S1 at 4 °C overnight after blocking with Blocking One reagent (Nacalai Tesque) ...