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Citations for Bio-Rad :
151 - 200 of 397 citations for Flumoxef sodium impurity P since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Excess dye was removed by two consecutive buffer exchanges through Micro Bio-Spin P-6 desalting columns (Bio-Rad). Protein concentration and labeling efficiency were estimated using a NanoDrop Microvolume Spectrophotometer.
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bioRxiv - Microbiology 2022Quote: All proteins were buffer exchanged into 0.2 M ammonium acetate using Bio-Spin P-6 Size Exclu-sion Spin Columns (BioRad) as previously described47 ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.4) in D2O using Micro Bio-Spin® Columns with Bio-Gel® P-6 (Bio-Rad Laboratories). Protein (final concentration 20 µM ...
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bioRxiv - Biochemistry 2021Quote: ... Unincorporated nucleotides were removed from the aqueous phase by using a P-30 Microspin column (RNase Free; Bio-Rad), and the oligonucleotide concentration was measured by using a Qubit ssDNA assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... for 30 minutes and then purified using a Micro Bio-Spin P-30 Gel Column (7326251, from Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... pH 8.3) and transferred onto PVDF membranes (Amersham Hybond P Low Fluorescence) with a tank transfer system (Bio-Rad) overnight at 20 V ...
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bioRxiv - Genomics 2023Quote: ... Excess dye was removed using Micro Bio-Spin® Columns with Bio-Gel® P-6 (cat:7326221, BioRad) by centrifugation at 1000g ...
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bioRxiv - Immunology 2023Quote: ... Buffer exchange was performed using Bio-Rad P-30 columns according to the manufacturer’s instructions (Bio-Rad, Catalog #7326231). After elution ...
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bioRxiv - Immunology 2021Quote: ... Proteins were also run on a sodium dodecyl sulphate (SDS) polyacrylamide gels (5–20% gradient; Bio-Rad) to check for purity39,40 ...
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bioRxiv - Cancer Biology 2020Quote: ... for smaller pellets 50 µl 1% SDS (Sodium Dodecyl Sulfate, Bio-Rad Laboratories Inc., Hercules, CA, USA) was used.
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bioRxiv - Microbiology 2020Quote: ... separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis on 5– 20% gels (Bio-Rad Ready Gel J), and transferred to polyvinylidene fluoride membranes (Immobilon-PSQ ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of denaturated protein in Laemmli buffer were loaded on 12% Sodium dodecyl sulfate gels (BioRad) and blotted on nitrocellulose membranes (Invitrogen) ...
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bioRxiv - Biophysics 2020Quote: Denaturing sodium dodecylsulfate (SDS) PAGE gels used in this study were prepared on Miniprotean III systems (BioRad) with a constant separating-gel pore size of 18% acrylamide (w/v) ...
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bioRxiv - Neuroscience 2023Quote: ... eluted by boiling in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) loading buffer (1610737, Bio-Rad), and analyzed by western blotting.
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody staining was done in 2% milk-TBST (+ 0.01% sodium azide) and detected using ChemiDoc (Biorad).
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bioRxiv - Cancer Biology 2024Quote: ... Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with Mini-PROTEAN TGX gels (Bio-rad) using 100V for 1.5 hours ...
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bioRxiv - Biophysics 2021Quote: ... The uncoupled BG-GLA-NHS was removed by filtration on Micro Bio-Spin™ P-6 Gel Columns (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: DNA isolated from cells by phenol:chloroform isolation and ethanol precipitation was cleaned on Micro Bio-Spin P-6 SSC columns (Bio-Rad) according to manufacturer protocol ...
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bioRxiv - Biochemistry 2021Quote: ... pH 5.3 (murine P-domains) or pH 7.3 (GII.4 Saga P-domain) at 4 °C via Micro Bio-Spin 6 columns (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... Fractions containing protein were pooled and desalted to remove imidazole by gel filtration on a 50 mL BioScale P-6 column (BioRad) using resuspension buffer as the eluant.
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bioRxiv - Biochemistry 2020Quote: The protein storage buffer for purified Pol IV and Pol IVT120P was exchanged to 10 mM potassium phosphate (pH 7.5) with Micro Bio-Spin P-30 Chromatography Columns (Bio-RAD) right before the measurements ...
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bioRxiv - Microbiology 2020Quote: ... Transcripts and labelled oligonucleotides to be used as a size indication were purified using P-30 Bio-Gel spin columns (Biorad). All reactions were stopped in gel loading buffer II (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... were handpicked to match size of islets across the samples and loaded into the polycarbonate perifusion chamber between two layers of polyacrylamide-microbead slurry (Bio-Gel P-4, BioRad) by the same experienced operator ...
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bioRxiv - Microbiology 2021Quote: ... for 60 min at 37°C followed by purification on a Micro-Bio Spin P-6 Gel Column (Bio-Rad). 1 pmol of 5’-end-radiolabeled primer was used for each extension reaction ...
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bioRxiv - Cell Biology 2021Quote: ... The S and P samples were solubilized by addition of an equal volume of 2X Laemmli SDS-PAGE sample buffer (Biorad) and boiling for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... were exchanged from size exclusion buffer into 2 M ammonium acetate pH 7.4 via a single-pass through a Bio-Spin P-6 column (Bio-Rad). Buffer-exchanged protein was loaded into gold-coated glass capillaries (1.0 mm OD/0.75 mm inner diameter with filament) ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 75 islets were sandwiched in a chamber between two layers of Bio-Gel P-2 (Bio-Rad, Cat#1504118) bead solution (200 mg beads/ml in KRB buffer) ...
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bioRxiv - Molecular Biology 2022Quote: ... and P-TEFb OE and wild-type (w1118) chromatin (CycT) were analyzed by qPCR on a CFX96 Real-Time System (BioRad). qPCR reactions were carried out using 2 μl of ChIP DNA as a template with 300 nM primers and 5X HOT FIREPol® EvaGreen® qPCR Mix Plus (Solis BioDyne ...
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bioRxiv - Biochemistry 2024Quote: ... and DTT was subsequently removed using a “Micro Bio-Spin® Columns with Bio-Gel® P-30” (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated by dual Tris-glycine SDS-PAGE (lower quarter 15%, upper ¾ 10% acrylamide gel and transferred to a P-type PVDF membrane using the BioRad wet transfer system ...
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bioRxiv - Plant Biology 2024Quote: ... was run at 120 V for 1:30 h and blotted to polyvinylidene fluoride (PVDF) membrane (Hybond P 0.45, Amersham, Cytiva, Marlborough, USA) in a Trans-Blot SD Semi-Dry Electrophoretic Cell (Bio-Rad) for 1.15 h with 1.5 mA/cm2 ...
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bioRxiv - Immunology 2024Quote: ... the SDS-PAGE gels were transferred to an Amersham Hybond P Low fluorescence 0.2 μm PVDF membrane (Cytiva) using the Trans-blot SD semidry transfer cell (Bio-Rad) (25 V ...
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bioRxiv - Molecular Biology 2024Quote: ... Fragmentation was stopped by addition of 80 μL 1 M Tris pH 6.8 and cleaned up twice with Micro Bio-Spin P-30 Gel Columns (BioRad, 7326223) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Size Exclusion chromatography was performed using a column packed with Bio-Gel P-2 fine resins (Bio-Rad, Hercules, CA). HPLC analysis of compounds was performed on a Shimadzu LC-20A with a Waters XBridge BEH ...
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bioRxiv - Biochemistry 2024Quote: ... and then buffer exchanged into 150 mM ammonium acetate pH 7.5 by two consecutive passes over centrifugal gel filtration columns (Micro Bio-Spin P-6, 6-kDa cut off; BioRad). Protein concentration was verified by A280 ...
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bioRxiv - Cell Biology 2024Quote: ... BioRep Diabetes) by sandwiching them between two layers of hydrated Bio-Gel P-4 polyacrylamide beads (150-4124, Bio-rad). These chambers were attached to a high-precision eight-channel dispenser pump (ISM931C ...
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bioRxiv - Bioengineering 2022Quote: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) was performed with MiniPROTEAN® Tetra Vertical Electrophoresis Cells (BioRad) using mini-PROTEAN® TGX™ precast gels (BioRad) ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: ... Protein extracts were separated by denaturing sodium dodecyl sulfate polyacrylamide gel electrophoresis (Bio-Rad Laboratories, Hercules, California, US) under reducing conditions and transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was twice washed with 2X SSC (0.15 M NaCl and 0.015 M sodium citrate) and 0.5 % SDS (Bio-Rad) for 30 minutes at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Reagents and protein markers used in sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) were obtained from BioRad, India ...
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bioRxiv - Microbiology 2024Quote: Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE (12% gel; Bio-Rad Laboratories, #4561043), visualized using InstantBlue Coomassie dye (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... The worm lysates were separated on 4–15% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS PAGE) (Bio-Rad). The proteins were transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... Protein (5-10 µg) was separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad, USA) in 26-well 4–15% Criterion™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2024Quote: The protein samples were mixed with 10x Sodium dodecyl-sulfate (SDS) loading buffer (Bio-rad 1610747, CA, USA) and heated at 95 °C for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... The sample with fragmented RNA was neutralized with 1M Tris-HCl pH 6.8 and isolated by passing through P-30 column (Biorad, #732-6250). The NRO-reaction products containing biotinylated RNA was purified using Streptavidin C1 beads which were washed thrice with wash buffer (0.1N NaOH ...
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bioRxiv - Developmental Biology 2022Quote: ... GEM-RT was performed in a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad; P/N 1851197): 53°C for 45 min ...
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bioRxiv - Microbiology 2021Quote: ... 15 mg/mL enoxaparin sodium derived from porcine intestinal mucosa (Sanofi-Aventis U.S., Bridgewater, NJ) was applied to a Bio-Gel P-10 column (2.5X120 cm, Bio-Rad, Hercules CA) and eluted with 0.2 M NH4HCO3 at a flow rate of 14 ml/h ...
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bioRxiv - Microbiology 2020Quote: ... followed by ExtrAvidin alkaline phosphatase conjugate and alkaline phosphatase substrate: p-nitro blue tetrazolium chloride enhanced 5-bromo-4chloro-3-indolyl phosphate (Bio-Rad). The membranes with the transferred rgp120 were incubated with the HRP-conjugated V5 antibody (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... inside an anaerobic glove-box and the excess DTT was completely removed via a Bio-Gel P-6 DG gel-desalting column (Bio-Rad) in thoroughly de-oxygenated MOPS buffer (20 mM ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction mix was incubated for 30 mins at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad).