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Citations for Bio-Rad :
2701 - 2750 of 7082 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected after centrifugation at 15,000 ×g for 15 min at 4°C and applied to a 20 mL open column (Poly-Prep Chromatography Columns; BIO-RAD) containing 500 µL of Ni-NTA agarose (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... fPanx1ΔC purity was assessed by SDS–polyacrylamide gel electrophoresis (PAGE) using 4-20% pre-cast gradient Tris-glycine gel (Bio-Rad) stained with Simply Blue (SimpleBlue Safe Stain ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were then clarified through centrifugation (14’000 g, 20 minutes, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... The samples were separated on a pre-heated 45 cm sequencing gel (45 °C, 65 W, 1 h) and visualized using a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... 5’-ATG GGT TCG CAT GGT CCT AAT GAC ACA C-3’) (Cousineau et al., 2022) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 µL reaction contained 500 ng of total RNA ...
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bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were incubated overnight at 4°C with primary antibodies and then probed with secondary antibodies before ECL development and imaging (Bio-Rad). Primary antibodies used for immunoblotting are as follows ...
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bioRxiv - Cell Biology 2022Quote: ... denatured in 2x Laemmli buffer (5 min at +95°C) and 20 µg were loaded per well of Mini-PROTEAN Precast Gel (Bio-Rad). The protein transfer was done with the Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with primary and HRP-secondary antibodies in TBST buffer with 5% milk (RT for 1 hour or overnight at 4 °C) and revealed with an ECL solution (BIO-Rad) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated at 95 °C for 5 minutes prior to being resolved on a 4 – 15% Mini-PROTEAN TGX Precast Protein Gels (Bio-rad) and transferred onto a methanol activated Immobilon-P PVDF membrane (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with beta-mercaptoethanol and denaturated at 95 °C for 5 min before loading on a 12 % PAA precast gel (#4561044, Bio-Rad). The gel was run at 70 V for 15 min and then 100 V for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Samples were heated at 95°C for 3 minutes and run on a 8 – 16% or 4 – 20% TGX-Criterion gel (Bio-Rad). The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were centrifuged at 20000 g for 20 min at 4 °C and then filtered by gravity using Poly-Prep chromatography columns (731-1550, Bio-Rad). Supernatants were filtered by gravity through Poly-Prep chromatography columns (731-1550 ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... The RNA sample was denatured by incubating it at 70°C for 2 min in S1000TM or C1000TM Thermal Cycler PCR machine (Bio-Rad). The denatured RNA was quickly transferred on ice ...
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bioRxiv - Immunology 2020Quote: ... Protein for immunoblotting was eluted from 25% of beads by heating at 95°C for 5 minutes in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from the remaining 75% of beads using TRIzol (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... After rinses with PBS-0.1% Tween 20 (PBST), sections were incubated overnight at 4°C with rabbit anti-Iba1 (Wako, 019-19741) and anti-BrdU (Biorad, MCA2060). After washes with PBST ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were boiled for 5 min at 95°C in 1x XT sample buffer containing 1x XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT precast gels (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: Protein samples were denatured for 5 min at 95 °C and run on Mini or Midi TGX-Stain-free precast gels (Bio-rad) at 110V for 60 min (mini gels ...
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bioRxiv - Neuroscience 2021Quote: ... and then heated to 37°C for 30 min and separated via SDS-PAGE on a Mini-Gel apparatus (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... The reaction mix was incubated for 30 mins at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad).
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bioRxiv - Pathology 2022Quote: ... 2-5%β-mercapto-ethanol for 10min at 70°C and then migrated with protein ladder (Precision plus protein all blue standards, Bio-Rad) in Bio-Rad gels (Criterion TGX ...
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bioRxiv - Synthetic Biology 2022Quote: ... Reaction mixtures were incubated for 2 hours at 25 °C and quenched by adding 10 μL 4x Laemmli Sample buffer (Bio-Rad). Samples were boiled at 95 °C for 5 min and each 8 μL were loaded onto 12% SDS-PAGE gels ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in 10% neutral buffered formalin at 4°C overnight and then mCherry-positive plaques were visualized using a ChemiDoc MP imaging system (Bio-Rad). Afterwards ...
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bioRxiv - Microbiology 2022Quote: ... membranes were incubated at 37°C for 1 h with goat anti-mouse IgG StarBright Blue 520 or anti-rabbit IgG Starbright Blue 700 (Bio-Rad) secondary antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... heated at 95 °C for 5 min and resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad). Proteins on the gel were transferred to the PVDF membrane ...
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bioRxiv - Neuroscience 2019Quote: ... and then eluted by boiling in SDS lysis buffer for 5 minutes at 95°C and resolved by SDS-PAGE (Bio-rad) gel ...
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bioRxiv - Plant Biology 2020Quote: ... heated for 30 min at 37°C and loaded into a polyacrylamide gel (any kD™ precast protein gel, Biorad #4569034). To avoid cross-contamination ...
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bioRxiv - Cell Biology 2019Quote: ... After centrifugation at 14000 rpm for 8min at 4° C the supernatant was collected and protein concentration determined by the Bradford protein assay (Bio-Rad). The proteins were run on 7%-15% SDS-PAGE gels (50μg per lane ...
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bioRxiv - Developmental Biology 2019Quote: ... A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad). Membranes were incubated overnight with one of the following rabbit primary antibodies (all from Cell Signaling Technology and all cross-react with rat) ...
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bioRxiv - Biochemistry 2020Quote: ... in 1X TBE buffer at 220V for ∼13 min at 4°C and were imaged with a Gel Doc XR+ (Bio-RAD) and Quantity One 4.6.9 software (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Protein extract was then added to the mixture and gently rotated at 4°C overnight then beads were washed 4 times with CHAPS buffer and then boiled in Laemmli Sample Buffer (Bio-Rad) for 10 min ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... The incubation was terminated by boiling the samples at 95°C for 5 minutes in 1X Laemmli sample buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... we used 40 cycles of denaturation at 94° C for 15 seconds followed by annealing/elongation at 60° C for 30 seconds in a C1000 thermal cycler CFX96 real-time system (Bio-Rad). We normalized gene expression to levels of β-actin mRNA ...
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bioRxiv - Genomics 2019Quote: All protein isolation steps were performed at 4 °C or on ice and protein concentrations were assessed with the Bradford assay (Bio-Rad). Protein from total pancreas lysates was isolated from a small fragment of mouse pancreas and minced in lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The second-dimension gel electrophoresis was performed in 1× TBE buffer containing 0.3 μg/ml of ethidium bromide at 6.0 V/cm for 5 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). DNA was transferred to Hybond-XL (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The formaldehyde crosslinks were reversed by heating the samples for 1 h at 70 °C and RNA was extracted using PureZOL™ (BioRAD) as described in the RNA extraction section ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris was cleared by centrifugation at 21000 g for 5 min at 4°C and supernatant was mixed with one-third volume 4X Laemmli Sample Buffer (Bio-Rad). For lysozyme lysis ...
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bioRxiv - Microbiology 2021Quote: ... proteins were denaturized in SDS-PAGE protein loading buffer for 5 minutes at 95°C and resolved by SDS-PAGE on 4-12% bis-tris Criterion XT Precast gels (Bio-rad), transferred to a PVDF membrane and blocked for at least 1h in 1% Casein blocking buffer (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... according to the respective manufacturer’s protocols with an annealing temperature of 58 °C during real-time PCR in a CFX96 real-time system (Bio-Rad) thermocycler.
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bioRxiv - Synthetic Biology 2021Quote: ... microwells containing assembled protocell arrays were all incubated at 37 °C for 3 hours before imaging on a ChemiDoc MP (Bio-Rad) imaging system ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of each protein sample was denatured and reduced by boiling at 95°C for 5 min in 10% (v/v) 2-mercaptoethanol-containing Laemmli sample buffer (Bio-Rad). Samples were resolved by SDS-PAGE with a precast Tris-glycine gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... at 1.0 V/cm for ∼48 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). The buffer was changed every ∼24 hr.
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bioRxiv - Neuroscience 2019Quote: ... Dynabead-antibody complexes were washed 3x with 1 ml IP buffer and 3x with 1X PBS and proteins were eluted at 70°C for 10 min in 2X Laemmli sample buffer (50 μl; Bio-Rad) containing 5% 2-mercaptoethanol ...
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bioRxiv - Pathology 2021Quote: ... 0.10 M) at 50°C overnight followed by cleanup on 150 μL cation exchange media (AG50WX8, Bio-Rad, Hercules, CA, US) on top of C18 SPE columns (Strata C18-E ...
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bioRxiv - Biochemistry 2019Quote: ... was transferred to a new tube with 50 μL 2 M HCl and boiled for 2 hours at 95°C in a C1000 thermocycler (Bio-Rad) to hydrolyze all glucans to glucose ...
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bioRxiv - Cell Biology 2021Quote: ... against the OAD buffer for 6h at 4 °C to remove the sucrose and then loaded on an EnrichQ 5/50 column (Bio-Rad) equilibrated with the ion-exchange buffer A (50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... After 24 h incubation at 4 °C the chelating agent was removed using a desalting column (Econo-Pac 10DG from Bio-Rad), pre-equilibrated with 20 mM Hepes buffer ...
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bioRxiv - Biochemistry 2021Quote: ... was incubated at 55°C for 2 h and then purified by 6% non-denaturing PAGE using a Prep Cell apparatus (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... The reaction was incubated at 37°C for 30 minutes then left to cool for 10 minutes before adding 6 μL of 0.2% 2-mercaptoethanol in SDS-page loading dye.38 Samples were then heated to 94°C for 2 minutes and added to the wells of a pre-cast 12% polyacrylamide gel (Bio-Rad). The gel was run for 45 minutes at 120 V in running buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...