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Citations for Bio-Rad :
2651 - 2700 of 7082 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... 10 million induced cells from each track were washed and labelled for HA and c-Myc detection as described above and sorted on an S3e cell sorter (BioRad) to isolate cells that show HA detection ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then cleared by centrifugation for 15 minutes at 14,000 rpm at 4°C and subjected to immunoprecipitation with anti-V5 mouse monoclonal antibody (#MCA1360, Bio-Rad) overnight at 4°C with rotation in DNA/RNA LoBind tubes ...
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bioRxiv - Molecular Biology 2024Quote: ... 60 mM EDTA pH 7.0 containing 5% β-mercaptoethanol and 1 mg/ml zymolyase 100T) at 40 °C and aliquoted to plug molds (BioRad) to prepare 20 agarose plugs ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples (25 µl total volume) were heated 5 minutes at 95 °C and loaded onto an AnyKD precast MiniProtean TGX Stain Free gel (Biorad) or an 15% SDS-PAGE gel ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were cleared at 5000g for 5min at 4°C and protein concentration was quantified by the Bradford assay (BioRad). Finally ...
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bioRxiv - Immunology 2023Quote: ... The second kit used was the Bio-Plex Pro Serology Reagent Kit (Bio-Rad, Cat No. 12014777), with human IgA positive and negative controls (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were mixed with SDS sample buffer containing DTT and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4%–15% Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Transfer to nitrocellulose membranes and probing for Western blot analysis was as described previously ...
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bioRxiv - Evolutionary Biology 2020Quote: Samples were heated at 95°C for 10 minutes before loading into 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) and running at 150V for approximately 90 minutes at 4°C in 1X tris-gylcine running buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... which is pre-equilibrated by Buffer A before use with rotation at 3 hr in 4°C in Econo-Pac Chromatography Columns (20 mL, Bio-Rad). After the unbounded fraction was discarded ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Protein samples were heated at 55°C for 10 min and 10 μl was loaded on SDS–PAGE (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad) and analyzed by Western blot.
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bioRxiv - Genetics 2019Quote: ... and heated at 90°C for 5 min before being run on a stain free gel and imaged by GelDoc imager (BIO-RAD).
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 5 minutes at 95°C before separation on a gradient 4-20% SDS-PAGE gel (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for semi-dry transfer (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad, USA) followed by chilling on ice ...
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bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was then added and the solution was mixed at 4°C for 10 minutes before addition of 100 mg of Biobeads SM2 (Bio-Rad). Biobeads were washed into methanol ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleavage-related change in fluorescence signal of the probe was measured every 30 s for 60 min under incubation at 37°C using a Real-time PCR system (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 2-3 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were fully resuspended by vortexing and incubated at 55°C for 15 minutes in a C1000 Touch thermal cycler (BIO-RAD) then re-pelleted with the magnet ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μl of the cell suspension was spread on a single partitioned YPDA plate and incubated at 30°C for 48 h prior to imaging on a ChemiDoc imaging system (Bio-Rad). The area of all colonies ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
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bioRxiv - Immunology 2019Quote: ... 40 cycles of reaction including denaturation at 94° C for 15 sec followed by annealing at 60° C for 30 sec were carried out using C1000 thermal cycler CFX96 real-time system (Bio-Rad). Gene expression was normalized to beta actin ...
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bioRxiv - Physiology 2019Quote: ... paraffin-embedded sections were incubated at 4°C overnight with antibodies for F4/80 (MCA497G, Bio-Rad Laboratories, Hercules, CA, USA), HILPDA (sc-137518 HIG2 Antibody (C-14) ...
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bioRxiv - Pathology 2019Quote: ... 20% methanol and MilliQ water) overnight at 4°C in a wet transfer apparatus at 70 mA constant voltage (Bio-Rad). Detection of the chemiluminescence immunochemical reaction was performed on a ChemiDoc ™ XRS + Molecular Imager® (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... 0.02% NaN3 and 1 mM DTT at pH 7.4 at 37°C in a thermal cycler (BioRad DNA Engine PTC-200). In some cases ...
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bioRxiv - Neuroscience 2019Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Bioengineering 2019Quote: Optimal MIP annealing temperature was determined to be 60.4 °C based on yield after circularization with a gradient of annealing temperatures ranging from 58–70 °C on a CFX96 Real-Time qPCR System (Bio-Rad). For all of these experiments ...
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bioRxiv - Bioengineering 2019Quote: ... qPCR was conducted for 50 cycles at an annealing temperature of 56 °C on a CFX Connect Real-Time PCR Detection System (Bio-Rad). A melt curve was generated at the end of the PCR reaction and a subset of reactions were run on a 1% agarose gel to ensure that only one product of the expected size was amplified per primer pair ...
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bioRxiv - Microbiology 2019Quote: T1L virions (2×1012 particles/ml) were incubated for 5 min at 55°C in a S1000 thermal cycler (Bio-Rad). The total volume of the reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... The mixture was diluted to ~30 mL with sample buffer and stirred at 4°C for 30 min prior removal of C12E8 by adding 4 g of Bio-Beads SM-2 (Bio-Rad) in stages of 0.5 ...
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bioRxiv - Plant Biology 2020Quote: ... Liposomes were eluted from the beads by boiling at 95°C for five minutes after the addition of 10x sample reducing agent (Nupage, NP0009) and 4x Laemmli sample buffer (Biorad, #1610747).
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bioRxiv - Biochemistry 2021Quote: ... The melting curves were measured at 1°C/min at the FRET channel in triplicate measurements and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Biochemistry 2021Quote: ... 5 μg of lysate was heat denatured for 3 minutes at 95°C for 3 minutes and resolved on a pre-cast 4-15 % SDS-PAGE gels (Bio-Rad) at 200V for 45 minutes in 1X running buffer (25mM Tris pH 8.3 ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Protein was eluted from beads by heating for 5 min at 95 °C in 1X Laemmli Buffer (Bio-Rad, Hercules, CA). Retrieved protein was detected by standard immunoblotting techniques using the following antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer B + 150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer D ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was incubated for 90 minutes at 4°C before the resin was collected in a 20 mL disposable column (Bio-Rad) and washed with 100 mL Buffer C + 400 mM NaCl ...
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bioRxiv - Pathology 2021Quote: ... maximum power with 30 second intervals and the lysate obtained by ultracentrifugation at 100,000 g for 60 minutes, 4°C (CS 100FNX, HITACHI) resuspended in PBS and the concentration was measured by Bio-Rad protein assay dye reagent concentrate (BioRad) ...
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bioRxiv - Neuroscience 2021Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 72°C for 30 sec and followed by a melting curve) was performed in a CFX Real-Time Detection System (Bio-Rad) and relative expression calculated using the CFX Manager Software (Bio-Rad).
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bioRxiv - Plant Biology 2021Quote: ... and incubated on a rotary shaker for 60 min at 4°C before transfer to a Poly-Prep Chromatography Column (Bio-Rad). The column bed was washed with 40 ml of a wash buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The melting curves were measured at 1°C/min at the FRET channel and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... according to their molecular weight and transferred to a nitrocellulose Hybond-C membrane using an iBlot Gel Transfer Device (Bio-Rad) or using a wet-transfer system (if protein molecular weight >120kDa) ...
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bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were incubated with 2 ml of glutathione resin and rotated for 1 h at 4°C in a 25 ml disposable drip column (Bio-Rad). Settled resin was washed with 25 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were heated from 20 to 95 °C at the rate of 1 °C min-1 and fluorescence signals were monitored using quantitative real-time PCR (CFX96 Real-Time System, Bio-Rad). Each experiment was done in triplicate and the average values were taken for analysis ...
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bioRxiv - Microbiology 2020Quote: ... Proteins that bound to PI(3)P or control beads were eluted by boiling at 90–95 °C for 20 min in 70 μL 4x Laemmli sample buffer (Bio-Rad) and centrifuging at 20,000 g for 15 min ...