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Citations for Bio-Rad :
2301 - 2350 of 6070 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Protein transfer was performed on methanol-activated polyvinylidene fluoride (PVDF) membranes by Trans-Blot Turbo Transfer system (1704150; Bio-Rad). After transfer the membranes were blocked in 5% BSA in TBS with 0.1% Tween-20 for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred from gels to the PVDF membrane using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were incubated in 5% nonfat dried milk in 1X TBST (Tris-buffered saline ...
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bioRxiv - Neuroscience 2024Quote: ... The gel was transferred to a 0.22 μm nitrocellulose membrane (AmershamTM Protran, G10600080) through Mini Trans-Blot Electrophoretic Transfer Cell (BioRad, 1703930) for 60 min at 100 V ...
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bioRxiv - Microbiology 2024Quote: ... membrane for 30 min at 2.5 V and 25 mA using a semi-dry Trans-Blot Turbo system (Bio-Rad). Membranes were blocked by incubating them for two hours at room temperature (RT ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a nitrocellulose membrane with a Trans-Blot®Turbo™ Transfer Starter System (BioRad, 170-4155). For high molecular weight samples (SYNJ1-BirA* ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF membrane (Carl Roth, T830.1) by using the Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad). The membrane was blocked with 5 % BSA (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... Spinal cord ‘myelin’ and optic nerve samples were resolved on 4-12% Bis-Tris geld at 200 V and transferred onto PVDF membrane using either semi-dry method (Trans-blot; BioRad) or a wet gel transfer (Mini Trans-blot ...
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bioRxiv - Neuroscience 2024Quote: ... and the separated proteins were transferred onto PVDF membranes using a Trans-Blot Turbo semi-dry transfer system (Bio-Rad). Membranes were blocked using 3% BSA in TBS with 0.1% Tween20 (TBST) ...
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bioRxiv - Immunology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane using the Mini Trans-Blot® Cell and Criterion™ Blotter (Bio-Rad). Proteins on the membrane were stained with Ponceau S buffer ...
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bioRxiv - Microbiology 2024Quote: Proteins were separated on 12% mini-protean TGX stain-free precast gels and transferred to a PVDF membrane using the Trans-blot system as per manufacturer’s instructions (Bio-Rad). Membranes were blocked in 2.5% skim milk at room temperature for 30 min ...
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bioRxiv - Immunology 2024Quote: ... 7.5 µg of proteins were separated on polyacrylamide gel and then transferred on nitrocellulose membrane by the Trans-Blot dry system (Biorad). Membranes were blocked on milk (5% ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were subjected to SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked using 5% non-fat dried milk in TBS-T (0.1% Tween-20 in 1x TBS ...
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bioRxiv - Microbiology 2024Quote: ... or blotted onto a polyvinylidene fluoride (PVDF) membrane using the semi-wet Western blot Trans-Blot Turbo Transfer System (BioRad). Blocking was done overnight at 4°C using a 0.1% I-Block (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: Enzymes were extracted using a phosphate-based buffer (50 mM KH2PO4, 1 mM EDTA, 0.05 % Triton X-100) and quantified by Bradford assay (Bio-Rad #5000006, Bio-Rad, Hercules, CA, USA). Citrate synthase activity was measured spectrophotometrically as previously described106.
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bioRxiv - Neuroscience 2019Quote: ... or BIC (20 μM) were added to the culture and cells were harvested with a 1:1 mixture of 2X Laemmeli buffer (BioRad, 1610737, 1:20 beta-mercaptoethanol) and radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies for 1 hour at room temperature (anti-rabbit or anti-mouse, 1:10.000, Bio-Rad, anti-goat, 1:7500, Pierce). Antibody binding was detected by chemiluminescence(Clarity kit ...
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bioRxiv - Microbiology 2022Quote: ... reagent in 0.2 mL PCR 8-well strips and heated to 100 °C for 10 min (T100; BioRad, Australia) and used immediately ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: ... the purity of the final enriched enzyme was estimated by band density using Image Lab v6.1 Software (Bio-Rad Laboratories, Inc.); the SDS-PAGE of TmrA and lane density for purity analysis is shown in Figure S2 and protein yield is in Table S6 of SI Text 3.
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bioRxiv - Biophysics 2021Quote: ... fPanx1ΔC purity was assessed by SDS–polyacrylamide gel electrophoresis (PAGE) using 4-20% pre-cast gradient Tris-glycine gel (Bio-Rad) stained with Simply Blue (SimpleBlue Safe Stain ...
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bioRxiv - Microbiology 2020Quote: ... yield and level of purity were determined by SDS-PAGE analysis using 4–20 % or 12 % Mini-Protean TGX precast gels from Bio-Rad. Gels were stained with QC Colloidal stain (3 ng sensitivity ...
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bioRxiv - Bioengineering 2021Quote: ... Protein purity was evaluated by SDS-PAGE using 4–20% Mini- PROTEAN® TGX Stain-Free™ Gels (BioRad; Cat#17000435).
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bioRxiv - Neuroscience 2019Quote: ... Final purification was performed by size-exclusion chromatography and the purity of the proteins was assessed by 4%-15% SDS-PAGE gels (Bio-Rad) under reducing conditions ...
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bioRxiv - Synthetic Biology 2019Quote: ... The purity and size of the proteins were validated on SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C ...
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bioRxiv - Microbiology 2020Quote: ... Samples were checked for purity and presence of protein using 15% SDS-PAGE (Polyacrylamide gel electrophoresis, Mini Protean 3 System, Bio-Rad) and Coomassie Brilliant Blue (Merck ...
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bioRxiv - Biochemistry 2020Quote: ... The protein purity was analysed by SDS-PAGE using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad).
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bioRxiv - Immunology 2021Quote: Purified proteins were analysed for purity determination via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 4-20% gradient gel (BioRad – 4568094), with or without the presence of 2-mercaptoethanol as reducing agent.
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated by 12 % SDS-PAGE using mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein purity was analyzed by SDS-PAGE and the protein concentration was determined using the Bradford assay (QuickStartTM, Bio-Rad, Germany) with BSA as the protein standard (Zor & Selinger ...
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bioRxiv - Bioengineering 2023Quote: Purified protein samples were visualized to evaluate purity by SDS-PAGE using the Bio-Rad mini-protean system (Bio-Rad laboratories). Samples were mixed in a 1:1 ratio with 2x Laemmli sample buffer containing 50 mM 1,4-dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2022Quote: NKX2-5 homeodomain purity was evaluated through SDS-PAGE using 15 well Mini-PROTEAN TGX Precast Protein Gels® (Bio-Rad). Samples were prepared with 4X loading buffer containing β-mercaptoethanol (BME ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration and purity of extracted protein was determined using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, 500-0006) following manufacturer’ s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein (25 µg) were denatured 1:1 in 2X Laemmli buffer (BIO-RAD) at 95°C for 10 minutes then held on ice ...
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bioRxiv - Pathology 2021Quote: ... Protein samples were first diluted 1:1 with Laemmli sample buffer (Bio-Rad, Hemel Hempstead, UK) containing 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... followed by secondary antibodies (1:4000 donkey anti-goat, Abcam; 1:4000 goat anti-mouse, Biorad). Immunoblots were developed using SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... 20 uL cell suspension was diluted 1:1 with 0.4% Trypan Blue solution (Bio-Rad #1450021) and cells were counted by hemocytometer (RPE-1 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Each compartment of each sample was diluted 1:1 in Laemlli Sample Buffer (BioRad, Hercules, CA) mixed with 350mM DTT (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... monoclonal rat anti-F4/80 antibody (dilution 1:200) (clone Cl:A3-1, MCA497G, Biorad Laboratories, Germany) After multiple washes ...
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bioRxiv - Pathology 2024Quote: ... Protein samples were first diluted 1:1 with Laemmli sample buffer (Bio-Rad, Hemel Hempstead, UK) containing 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and collecting 1 mL fractions from the top at a pace of 1 mL per minute while continuously measuring A254 using the BioLogic LP system (BioRad, Hercules, California, USA).
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bioRxiv - Zoology 2019Quote: ... were used within one month of collection to determine total and host protein (mg mL−1) by the Bradford assay (Bradford 1976) against bovine serum albumin standards (Bio-Rad 500-0207). Readings were taken at 595 nm (EnSpire microplate reader MLD2300).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PPIases and PPIase fragments were vacuum aspirated onto a nitrocellulose membrane as serial dilution starting with 1 µg/ml using the dot blot system (Bio-Rad, Feldkirchen, Germany). Recombinant C3 toxin from C ...
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... and DiaClon Anti-D (Bio-Rad, Basel, Switzerland) were used ...
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bioRxiv - Bioengineering 2023Quote: ... 15 ug protein were loaded and separated using 4–15% Mini-PROTEAN® TGX™ Precast SDS-PAGE Gels (Bio-rad, #4561086). Samples were then transferred to nitrocellulose membranes for immunoblotting ...
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bioRxiv - Biochemistry 2020Quote: ... 8 % SDS-polyacrylamide (Biorad 29:1) gels were used for the full-length protein and 10 % SDS-polyacrylamide gels were used for the 95 aa reporter constructs ...
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bioRxiv - Molecular Biology 2020Quote: ... TGN46 at 1:1000 (Bio-Rad), for two hours at RT ...
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bioRxiv - Neuroscience 2020Quote: ... BrdU (Rat; 1:500; Bio-Rad). Alexa Fluor 488- ...
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bioRxiv - Neuroscience 2021Quote: ... anti-CD68 (1:500, Bio-Rad), and anti-Iba1 (1:500 ...