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Citations for Bio-Rad :
2101 - 2150 of 5665 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Gels were transferred to a PDVF membrane (Roth) using either the Trans-Blot SD Semi-Dry Transfer Cell (Bio-rad) or the Mini Trans-Blot Cell (Biorad) ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were transferred from the SDS-PAGE gel to Trans-Blot Turbo Midi 0.2 µm Nitrocellulose (Bio-Rad, 1704159) with Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... and then proteins were transferred to a nitrocellulose membrane using a wet Trans-Blot system (Bio-Rad, Hercules, CA, USA). The primary antibodies used were anti-slow MyHC (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... Protein separations were transferred onto a nitrocellulose membrane using the Trans-blot Turbo RTA transfer kit (Bio-rad, #170-4270) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were run on a 10% SDS-PAGE gel (30μg/lane) and transferred using a semi-dry blotting system (Trans-Blot Turbo system, Bio-Rad) for 10 minutes at 25V ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were anti-mouse (Bio-Rad, #170-6516, dilution 1:1000 or 1:2000) and anti-rabbit (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... 1:5000 and 1:10000 dilutions of the libraries in triplicate on iQ5 (Bio-Rad) and CFX96 Touch qPCR machines ...
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bioRxiv - Plant Biology 2021Quote: ... 0.05% SDS) and a semi dry transfer cell (Bio-Rad, 1 mA/cm2, 1 h). Membrane was blocked with protein free blocking solution T20 (Pierce ...
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bioRxiv - Microbiology 2019Quote: ... was diluted 1:5000 in 1% (w/v) casein blocking buffer (Bio-Rad, 161-0782). Blots were incubated at room temperature for 2 h ...
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bioRxiv - Immunology 2020Quote: ... A total of 50μg protein was diluted 1:1 with 2x Laemmli buffer (Bio-Rad), resolved by SDS-PAGE and transferred into PVDF membranes ...
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bioRxiv - Bioengineering 2021Quote: ... samples were mixed 1:1 with 2x Laemmli sample buffer with 2-mercaptoethanol (Bio-Rad) and boiled for 10min ...
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bioRxiv - Biochemistry 2021Quote: Purified protein was mixed in 1:1 ratio with 2X laemmli loading dye (Bio-rad) prepared according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1× iTaq Universal SYBR Green Supermix or 1× iTaq Universal Probe Supermix (Bio-Rad, USA), 300 nM each of forward primer (late RT product ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with the HRP-conjugated secondary antibodies (1:2500-1:10000, Bio-Rad) for 1h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were mixed 1:1 with 2X Laemmli Sample Buffer (Bio-Rad, Hercules, CA) (5% β-mercaptoethanol ...
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bioRxiv - Physiology 2023Quote: ... prepared at a 1:1 ratio and acquired using the ChemiDoc MP System (Bio-Rad).
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bioRxiv - Genetics 2022Quote: Samples taken from prior assays were mixed 1:1 with Laemmli Sample Buffer (Bio-Rad) with 10% 2-mercaptoethanol and vortexed to ensure adequate mixing ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with Laemmli Sample Buffer (1610737, BIO-RAD, Hercules, CA) and heated to 80°C for 30 min.
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bioRxiv - Immunology 2024Quote: Samples were prepared in 1 x Laemmli buffer with or without 1 x dithiothreitol (Biorad). Samples were then heated for 10 min at 95 °C and loaded onto a precast NuPAGE™ 4-12 % Bis-Tris polyacrylamide gel in NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: The removal of DDM in the protein solutions was done by fixing the protein concentration at 1 mg/ml and using 30 mg of BioBeads SM2 (Bio-Rad, CA) per mg of protein ...
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bioRxiv - Microbiology 2021Quote: ... Gels were stained with ethidium bromide (0.5 mg mL-1) and photographed using the Quantity One electrophoresis analysis system (Bio-Rad, CA, USA). Gel images were digitally normalized to a single DNA marker to reduce gel-to-gel banding pattern variability ...
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bioRxiv - Systems Biology 2021Quote: Samples were normalized to 1 mg/mL protein in 0.1% Azo by the Bradford assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006) using bovine serum albumin to generate a standard curve ...
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bioRxiv - Microbiology 2021Quote: ... the gel was stained with 1 mg/mL ethidium bromide and visualized with the ChemiDoc XRS system (Bio-Rad Laboratories, CA, USA). Genetic similarities were measured using the Dice coefficient and the clustering was carried out by the unweighted-pair group method with arithmetic averages (UPGMA ...
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bioRxiv - Microbiology 2020Quote: Two hundred µL of overnight bacterial cultures were transferred 5 mL of a 1:10 dilution of TSB medium (Bio-Rad, USA) supplemented with 1 mg mL-1 L-tryptophan and incubated at 30°C overnight (Gordon & Weber 1951) ...
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bioRxiv - Neuroscience 2021Quote: Autaptic murine striatal neurons (DIV 15-18) were incubated with 1 μg/ml human GABAAR mAb or control antibody (anti-CD52, Bio-Rad, #HCA175) at 37°C for 24 hours ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... before injecting into 1 mL Hitrap Heparin HP column pre-equilibrated with Wash Buffer C operated in the BioLogic DuoFlow™ FPLC system (Bio-rad). The protein was eluted from the column by running a gradient flow rate that exchanges Wash Buffer C and Elution Buffer D (2000 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... Fraction collection was performed using a Masterflex L/S easy-load II (Cole Parmer, 77200-50) running at approx 1 mL/min and BioFrac™ fraction collector (Bio-Rad) with a fraction time window of 2 min ...
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bioRxiv - Plant Biology 2023Quote: ... a total of 20 µL of 1 µg/µL plasmid DNA was combined with 50 µL of gold microparticles (1 µm, 50 mg/ml, suspended in 50% (v/v) sterile glycerol) (#1652263, Bio-Rad, USA), 100 µL 2.5 M CaCl2 and 40 µL 0.1 M spermidine (S0266 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The blocking buffer was removed and 10 ml 5% BSA-PBST with 1 μl RABBIT anti-DYKDDDDK Tag antibody (Bio-Rad, # AHP1074), 1 μl anti-MBP Monoclonal Antibody (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15 ug of protein lysates were run on 4– 20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-Rad) and then transferred to polyvinylidene difluoride membranes ...
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bioRxiv - Neuroscience 2023Quote: ... Protein (10 ug) from each sample was treated with ß-mercaptoethanol and subsequently electrophoresed on precast 4%–10% gradient gels (Biorad), as previously described (Alcantara et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 10 ug of protein from cortical samples and 45ug of muscle samples were diluted in 4x Laemmli sample buffer (Bio-Rad). P3 brain samples were already directly resuspended in 4x Laemmli sample buffer ...
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bioRxiv - Physiology 2023Quote: Enzymes were extracted using a phosphate-based buffer (50 mM KH2PO4, 1 mM EDTA, 0.05 % Triton X-100) and quantified by Bradford assay (Bio-Rad #5000006, Bio-Rad, Hercules, CA, USA). Citrate synthase activity was measured spectrophotometrically as previously described106.
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bioRxiv - Neuroscience 2019Quote: ... or BIC (20 μM) were added to the culture and cells were harvested with a 1:1 mixture of 2X Laemmeli buffer (BioRad, 1610737, 1:20 beta-mercaptoethanol) and radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated with secondary antibodies for 1 hour at room temperature (anti-rabbit or anti-mouse, 1:10.000, Bio-Rad, anti-goat, 1:7500, Pierce). Antibody binding was detected by chemiluminescence(Clarity kit ...
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bioRxiv - Microbiology 2022Quote: ... reagent in 0.2 mL PCR 8-well strips and heated to 100 °C for 10 min (T100; BioRad, Australia) and used immediately ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: ... the purity of the final enriched enzyme was estimated by band density using Image Lab v6.1 Software (Bio-Rad Laboratories, Inc.); the SDS-PAGE of TmrA and lane density for purity analysis is shown in Figure S2 and protein yield is in Table S6 of SI Text 3.
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bioRxiv - Biophysics 2021Quote: ... fPanx1ΔC purity was assessed by SDS–polyacrylamide gel electrophoresis (PAGE) using 4-20% pre-cast gradient Tris-glycine gel (Bio-Rad) stained with Simply Blue (SimpleBlue Safe Stain ...
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bioRxiv - Microbiology 2020Quote: ... yield and level of purity were determined by SDS-PAGE analysis using 4–20 % or 12 % Mini-Protean TGX precast gels from Bio-Rad. Gels were stained with QC Colloidal stain (3 ng sensitivity ...
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bioRxiv - Bioengineering 2021Quote: ... Protein purity was evaluated by SDS-PAGE using 4–20% Mini- PROTEAN® TGX Stain-Free™ Gels (BioRad; Cat#17000435).
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bioRxiv - Neuroscience 2019Quote: ... Final purification was performed by size-exclusion chromatography and the purity of the proteins was assessed by 4%-15% SDS-PAGE gels (Bio-Rad) under reducing conditions ...
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bioRxiv - Synthetic Biology 2019Quote: ... The purity and size of the proteins were validated on SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C ...
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bioRxiv - Microbiology 2020Quote: ... Samples were checked for purity and presence of protein using 15% SDS-PAGE (Polyacrylamide gel electrophoresis, Mini Protean 3 System, Bio-Rad) and Coomassie Brilliant Blue (Merck ...
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bioRxiv - Biochemistry 2020Quote: ... The protein purity was analysed by SDS-PAGE using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad).
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bioRxiv - Immunology 2021Quote: Purified proteins were analysed for purity determination via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 4-20% gradient gel (BioRad – 4568094), with or without the presence of 2-mercaptoethanol as reducing agent.
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bioRxiv - Biochemistry 2022Quote: ... Enzyme purity and molecular weight were estimated by 12 % SDS-PAGE using mini-PROTEAN 3 system (BioRad, Hercules, California, United States). Gels were stained using PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of the recombinant protein was evaluated by SDS-PAGE and Coomassie staining (ChemiDoc Imaging System; BioRad, Hercules, CA, USA), and the concentration of the purified recombinant protein was determined with a Bio-Rad protein assay kit using bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2023Quote: Purified protein samples were visualized to evaluate purity by SDS-PAGE using the Bio-Rad mini-protean system (Bio-Rad laboratories). Samples were mixed in a 1:1 ratio with 2x Laemmli sample buffer containing 50 mM 1,4-dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2022Quote: NKX2-5 homeodomain purity was evaluated through SDS-PAGE using 15 well Mini-PROTEAN TGX Precast Protein Gels® (Bio-Rad). Samples were prepared with 4X loading buffer containing β-mercaptoethanol (BME ...