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Citations for Bio-Rad :
2151 - 2200 of 5665 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Protein purity was analyzed by SDS-PAGE and the protein concentration was determined using the Bradford assay (QuickStartTM, Bio-Rad, Germany) with BSA as the protein standard (Zor & Selinger ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of protein (25 µg) were denatured 1:1 in 2X Laemmli buffer (BIO-RAD) at 95°C for 10 minutes then held on ice ...
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bioRxiv - Pathology 2021Quote: ... Protein samples were first diluted 1:1 with Laemmli sample buffer (Bio-Rad, Hemel Hempstead, UK) containing 5% β-mercaptoethanol (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... followed by secondary antibodies (1:4000 donkey anti-goat, Abcam; 1:4000 goat anti-mouse, Biorad). Immunoblots were developed using SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: ... 20 uL cell suspension was diluted 1:1 with 0.4% Trypan Blue solution (Bio-Rad #1450021) and cells were counted by hemocytometer (RPE-1 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Each compartment of each sample was diluted 1:1 in Laemlli Sample Buffer (BioRad, Hercules, CA) mixed with 350mM DTT (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... monoclonal rat anti-F4/80 antibody (dilution 1:200) (clone Cl:A3-1, MCA497G, Biorad Laboratories, Germany) After multiple washes ...
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bioRxiv - Microbiology 2022Quote: ... and collecting 1 mL fractions from the top at a pace of 1 mL per minute while continuously measuring A254 using the BioLogic LP system (BioRad, Hercules, California, USA).
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bioRxiv - Zoology 2019Quote: ... were used within one month of collection to determine total and host protein (mg mL−1) by the Bradford assay (Bradford 1976) against bovine serum albumin standards (Bio-Rad 500-0207). Readings were taken at 595 nm (EnSpire microplate reader MLD2300).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PPIases and PPIase fragments were vacuum aspirated onto a nitrocellulose membrane as serial dilution starting with 1 µg/ml using the dot blot system (Bio-Rad, Feldkirchen, Germany). Recombinant C3 toxin from C ...
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were resolved by SDS-PAGE on 10% or 15% Tris-Glycine buffered polyacrylamide gels and transferred to PVDF membranes using mini trans-blot module (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Transfer to a nitrocellulose membrane (Amersham Bioscience, Arlington Heights, IL, USA) was performed using a semi-dry apparatus (Trans-Blot SD, Bio-rad) and analysed by immunoblotting with enhanced chemiluminescence reagents (Amersham Bioscience ...
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bioRxiv - Genetics 2021Quote: ... was used to dry transfer the material onto a PVDF membrane (Trans-Blot Turbo Mini 0.2 ul PVDF Transfer Packs, Bio-Rad, #1704156). After blocking with 5% skim milk in TBS-T ...
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bioRxiv - Microbiology 2021Quote: ... Normalized volumes of samples from the different growth time points were applied on SDS-PAGE gels (10% acrylamide) and transferred to a nitrocellulose membrane using Mini Trans-Blot cells (Bio-Rad Laboratories Ltd. ...
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bioRxiv - Physiology 2022Quote: ... Transfer Packs and protein transfer onto membranes was achieved using the low or mixed molecular weight (MW) preprogrammed protocol in a Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked for 1 hour at room temperature in 5% milk or 5% BSA (Millipore Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... For western blotting, separated proteins were transferred onto a nitrocellulose membrane (Whatman, Dassel, Germany) using a Trans-Blot Turbo transfer system (Bio-Rad). The membrane was blocked and then incubated for 1 h with an His-tag-specific mouse mAb antibody (Proteintech ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes (Amersham Protran) for 30 min at 15V with a Trans-Blot SD semidry transfer cell (Bio-Rad) or in transfer buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded on SDS-polyacrylamide gels (8 to 10 %) and transferred to nitrocellulose membranes using Trans-blot turbo transfer system (Bio-Rad). Membranes were saturated for 1 h with TBST buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto a pre-activated PVDF membrane using the Trans-Blot Turbo transfer system (BIO-RAD). Membranes were blocked with 5% non-fat milk in tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Immunology 2021Quote: Recombinant human GFAP protein were run on 12% SDS-PAGE and transferred onto membrane via Trans-Blot Turbo Transfer System (BIO-RAD). Membrane was incubated in blocking buffer (PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was transferred onto an Amersham Hybond-NX nylon membrane with a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) by applying 20 V for 1 h and then fixed using the EDC-mediated (1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Neuroscience 2019Quote: ... were separated on 4-20% SDS-polyacrylamide gel (Life technology) and transferred onto a nitrocellulose membrane by electroblotting using the Trans-Blot turbo transfer system (Bio-Rad). The membrane was blocked with blocking buffer (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... 5-15 µg of protein was resolved by SDS/PAGE and transferred to nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were blocked in 5% milk in phosphate buffered saline with 0.1% Tween (PBS-T ...
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bioRxiv - Cancer Biology 2019Quote: ... they were transferred from the gel to a PVDF membrane using a Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) and applying 250 mA ...
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bioRxiv - Genetics 2021Quote: ... The gels were blotted using the Bio-Rad Turbo Transfer System using Trans-Blot Turbo Mini NitroCellulose Transfer Packs (Bio-Rad). The blot was imaged for stain-free loading control after the transfer ...
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bioRxiv - Microbiology 2020Quote: ... The gel was blotted on a PVDF membrane using a semi-dry transfer unit (Trans-Blot Semi-Dry Electrophotetic transfer cell, Bio-Rad) at 25V for 45 minutes ...
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bioRxiv - Microbiology 2020Quote: ... cell lysates were resolved on 7.5% Mini-PROTEAN TGX SDS-PAGE gels and then transferred to polyvinylidene difluoride (PVDF) membranes using a Trans-Blot Turbo transfer system (Bio-Rad). Membranes were blocked with 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were subjected to SDS-PAGE and the proteins in the gel were transferred to a PBDF membrane using Trans-Blot Turbo Transfer System (Bio-Rad). After probing the membrane with the primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... All samples were boiled at 65°C for 10 minutes and then equal amounts of each sample were separated using SDS-PAGE and subsequently blotted on nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were washed in Tris-buffered saline-Tween (TBS-T ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto a polyvinylidene diflouride (PVDF) membrane using a Trans-Blot turbo transfer system (Bio-Rad). The membrane was blocked with 5% non-fat dried milk powder in Tris-buffered saline and 1% Tween 20 and probed with appropriate antisera ...
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bioRxiv - Microbiology 2021Quote: ... A 30µg protein extracts were subjected to denaturing 10% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membrane preactivated with absolute methanol using Trans-Blot Turbo Transfer System (Bio-Rad). After blocking with 5% powdered skim milk in 1% phosphate buffer solution plus 0.1% Tween-20 (PBST) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated by 12% SDS-PAGE and stained with Coomassie or transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked in 10% skimmed milk in TBS-Tween 0.05% (TBS-T) ...
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bioRxiv - Microbiology 2020Quote: ... resolved on Novex 4–20% tris–glycine gels and transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad). As a loading control ...
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bioRxiv - Microbiology 2020Quote: ... PCR product were separated by electrophoresis at 130 V for 30 min on 2% agarose gel in TAE buffer and visualized using SafeRed dye and trans-UV (302 nm) imaging in a ChemiDoc XRS+ (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated via SDS-PAGE and transferred to a PVDF membrane using Trans-Blot Turbo System (Bio-rad). The following antibodies were used for detection ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated on 12% or 15 % SDS-PAGE gels and transferred onto PVDF membranes using a Trans-Blot Turbo transfer system (Bio-Rad). Blocking was performed with 3% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... were then activated with 100% methanol and total protein extracts were transferred for 14 min at 25 V using a Trans-Blot Turbo Transfer System (Bio-Rad). Membrane-bound proteins were visualized with Ponceau S (78376 ...
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bioRxiv - Neuroscience 2022Quote: ... size-separated by SDS-PAGE in 4–20% Criterion TGX Precast gels and transferred using Trans-Blot Turbo Midi Nitrocellulose Transfer Packs (Bio-Rad). Membranes were blocked with 5% BSA in Tris-buffered saline/0.5 % Tween-20 (TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of total protein were resolved in 4-20% SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo transfer system (Bio-rad). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... 192 mM glycine and 20% of methanol) for 15 min before assembly in the Mini Trans-Blot transfer cell (BioRad 1703935). The transfer was done at 4°C for 16 h at 110 mA constant current ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were transferred to nitrocellulose membranes in a Turbo blotter (Trans-Blot Turbo Blotting System, 170-4155, Bio-Rad Laboratories). The total protein amount was visualized using the stain-free image of the blot (Figure 1B) ...
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bioRxiv - Neuroscience 2022Quote: ... and transferred to polyvinylidene difluoride membranes using a semi-dry Biorad transfer machine (Trans-Blot® Turbo TM Transfer System) with the 1X Transfer Buffer (BioRad) at 25 V for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... 20% (v/v) methanol) at a constant amperage of 300 mA for 90 min using a Mini Trans-Blot Cell (Bio-Rad). The membranes were incubated in NRA blocking buffer (INNOVAX ...
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bioRxiv - Immunology 2020Quote: ... Lysates (20–30 μL) were run on 8–10% polyacrylamide gels and transferred to PVDF membranes (Trans-Blot Turbo Transfer System, Bio-Rad). Membranes were blocked with 5% BSA in TBS (TBS ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... then separated via SDS-PAGE and transferred onto a PVDF membrane (18 min, 15 V) by Trans-Blot® SD semi-dry Western blot (BioRad). The membrane was exposed as described before overnight to a PhosphoImager screen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 20 μg of protein was separated using a 3–8% Criterion™ XT tris-acetate or a 4–15% Criterion™ XT tris-glycine protein gels and then transferred to PVDF membrane with a Trans-Blot Turbo Transfert system (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... gels were transferred to a polyvinylidene difluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, Hercules, CA). Membranes were then blocked in TBS-T (50 m mol/L Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... 20-30 μg of total protein was resolved on 8-16% TGX stain-free gels and transferred onto nitrocellulose membrane using the Trans-Blot Turbo RTA Transfer kit and the Trans-Blot Turbo Transfer system set to 7 min transfer protocol (all from Bio-Rad). Stain-free gel and blots were imaged using the Gel Doc EZ imaging system (Bio-Rad ...