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Citations for Bio-Rad :
2151 - 2200 of 6580 citations for Progesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 7% acetic acid for 30 minutes at room temperature followed by two 5-minute washes in Mill-Q water for imaging on the ChemiDoc (BioRad). Alternatively ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBS-T (3 × 5 minutes) and incubated in horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000) (BioRad) in blocking solution for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 40 cycles of 95 °C for 5 sec and 60 °C for 30 sec using the CFX 96 real-time systems (Biorad). The 2−ΔΔCt method was used to analyze the data (Schmittgen and Livak ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was then washed three times with TBS-T for 5 min each before developing using Clarity MaxTM Western ECL Substrate (1705062; BioRad) and imaged using a BioRad ChemiDoc XRS+.
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bioRxiv - Cell Biology 2023Quote: ... The oligonucleotide substrate products were visualized using the ChemiDoc MP Imaging System to detect Cy 5 fluorescence (Bio-Rad, 1708280).
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bioRxiv - Microbiology 2023Quote: ... Blot sections were washed 5 × 5minutes in TBST and signal was detected using Clarity Western ECL Substrate (BioRad 170 5060). Images were captured on a BioRad Chemi Doc XRS+ imager.
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bioRxiv - Cell Biology 2023Quote: ... the membrane was cut lengthwise into strips to isolate bands of particular molecular weights before being washed with TBST and incubated at room temperature for 1 H in a 5% blocking buffer solution (Biorad). The western blot strips were then incubated overnight at 4 C in a solution consisting of the primary antibody diluted into blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate secondary-HRP conjugated antibodies in 5% Milk TBST were incubated for 1 hr at room temperature prior to development with clarity ECL (BioRad) developing reagents with a ChemiDoc Touch (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... After 3x 10 min washes in TBST appropriate secondary-HRP conjugated antibodies in 5% Milk TBST were incubated for 1 hr at room temperature and developed with a ChemiDoc Touch (BioRad) as described above.
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Genetics 2023Quote: ... The beads were washed four times for 5 min with RIP buffer containing 0.5% sodium deoxycholate and boiled in 1xLaemmli Sample Buffer (Bio-Rad) for 10 min at 100°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μl of the sample were heated at 95°C for 5 min and resuspended in 4x Laemmli sample buffer (BioRad)/50 mM DTT before SDS-PAGE.
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bioRxiv - Molecular Biology 2023Quote: ... qPCR reactions were prepared from cDNA at 1:5 dilution with SYBR Green iTaq Universal SYBR Green Supermix (Biorad, 1725120) and qPCR primers at 10 µM in a total volume of 10 µL in a ≥ 40-cycle and melt curve reaction cycler (Biorad ...
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bioRxiv - Cancer Biology 2022Quote: ... 10-15 μg of protein samples and 3-5 μL of Precision Plus Protein Dual Color Standards (BIO-RAD, #1610374) were loaded in NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Whole-cell lysates for immunoblotting were prepared by dissolving cells in Laemmli Sample Buffer containing 5% 2-mercaptoethanol (Bio-Rad). Protein samples were loaded on 4-15% polyacrylamide gels (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... followed by three-times washing with TBST for 5 min and subsequent incubation with the goat anti-rabbit IgG (H+L)-HRP conjugate (BioRad) (diluted 1:3000 in TBST ...
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bioRxiv - Cell Biology 2023Quote: ... 150ul of the suspension was filtered through a 40μM cell strainer at 40G for 5 minutes to remove clumped cells and subsequently used for analysis on the ZE5 flow cytometer (Biorad). Analysis of gene expression was analyzed using the Easyflow MATLAB script (https://antebilab.github.io/easyflow/) ...
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bioRxiv - Immunology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 15 minutes at 50°C and 5 minutes at 85°C in T100 Thermal Cycler (1861096, Bio-Rad). Real-time qPCR reactions were performed using SsoAdvanced Universal SYBR® Green Supermix (1725274 ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were run for 5 min at 130 V followed by 55min min at 180 V (PowerPac Basic Power Supply (Biorad)) ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was then transferred to a 1 mm cuvette (ice chilled) for electroporation at 1.5 kV for 5 ms using an electroporator (Bio-Rad). Electroporated samples were mixed with 1 ml of SOC medium and transferred into a culture tube containing 1 ml of Brucella broth (BD) ...
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bioRxiv - Neuroscience 2023Quote: ... The samples adjusted to contain equal amounts of protein (10 μg or 12 μg) were next denatured (5 min at 95°C) in loading buffer (#1610747, BioRad) and run on 10% TGX (#4561034 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with secondary antibody goat anti-rabbit conjugated to HRP (1:5000, 5% wt/vol milk in TBS-T; #170-6515, BioRad) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 μl of this cell suspension was then placed on a 1.5% (w/v) low melting agarose (LMA; Bio-Rad) TGY2X pad prepared on a cover glass using a gene frame (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was diluted 1:4 and 4.3 µL of cDNA was mixed with 5 µL iTaq Universal SYBR Green Supermix (BioRad 1725124) and 0.7 µL of 5 µM forward and reverse qPCR primers targeting the gene of interest (333 nM final primer concentration ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95 °C for 5 minutes and separated on precast 4-20% Criterion TGX gels (Bio-Rad). The competition of IA-rhodamine was analyzed by in-gel fluorescent signal using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Detection of proteins was achieved following 5 min incubation with Enhanced Chemiluminescence (ECL) reagent and exposed using a ChemiDoc imaging system (BioRad). Loading control was performed by normalizing the target protein to the stain-free images of total protein ...
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bioRxiv - Biophysics 2023Quote: ... The blots were finally washed thrice with TBST with 5 minute incubations before adding the ECL substrate solution (1705061, Biorad). Chemiluminescence images were acquired on a Chemidoc™ imaging system (Biorad) ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were washed at least three times for 10 minutes with 1x TBST solution followed by 5 minutes incubation at room temperature with either Clarity Western ECL (BioRad). Immunoblots were developed using an Amersham ImageQuant 500 CCD imaging system (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were boiled at 95 °C for 5 min before being loaded onto a polyacrylamide gel (Cat# 4561094, Bio-Rad). Proteins were blotted onto a PVDF membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies to the membrane for 1 h in the presence of 5% milk followed by four washes in TBS-T and developing with ECL substrate (1705061; BioRad). Blot signal was captured using the same BioRad ChemiDoc™ Touch Gel Imager ...
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bioRxiv - Microbiology 2023Quote: ... All samples collected were heated to 95°C for 5 minutes before resolving on a 12% mini-PROTEAN precast gel (BioRad). A Western blot using polyclonal EssR antiserum was conducted following the protocol outlined in the Western blot method section below ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of protein was boiled for 5 min at 95°C and then loaded on a 4-15% SDS gel (Biorad).
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bioRxiv - Genetics 2024Quote: cDNA was generated using 5 ng of total RNA using iScript Reverse Transcriptase supermix (cat#1708841) and C1000 Touch Thermal Cycler (Biorad) as per manufacturer guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3 times for 5 min with 1X TBS 0.1% Tween 20 and unsaturated fluorescent signal was acquired using Chemidoc MP imager (Bio-Rad) with identical settings for samples to be compared within an experiment.
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bioRxiv - Cancer Biology 2024Quote: ... and RT-qPCR performed on the resulting cDNA (diluted 5-fold) using the iQ SYBR Green Supermix (Bio-Rad, #1708882) and following the manufactures instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and ∼140-150nt DNA fragments were then size-selected by gel electrophoresis with 5% TBE gels (Bio-Rad, Cat #4565015) following NEB library prep kit protocol ...
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bioRxiv - Neuroscience 2024Quote: ... boiled for 5 min at 95 °C and then loaded on 4–15% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Gels were transferred onto polyvinylidene fluoride membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were washed four times with PBS-T for 5 minutes and chemiluminescent signals were detected using Clarity Western ECL Substrate (BioRad,), and visualised using the ImageQuant® LAS-4000 imager (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... before heating at 95°C for 5 minutes and finally loaded onto 4-20% polyacrylamide gel (Bio-Rad, Cat # 4561094). Samples were then transferred to a PVDF membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... Stained cells were resuspended in FACS buffer and acquired with a 5-laser Bio-Rad ZE5 flow cytometer (Bio-Rad). FACS data were analyzed using FlowJo version 10.9 software (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... 60 mM EDTA pH 7.0 containing 5% β-mercaptoethanol and 1 mg/ml zymolyase 100T) at 40 °C and aliquoted to plug molds (BioRad) to prepare 20 agarose plugs ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples (25 µl total volume) were heated 5 minutes at 95 °C and loaded onto an AnyKD precast MiniProtean TGX Stain Free gel (Biorad) or an 15% SDS-PAGE gel ...
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bioRxiv - Genetics 2019Quote: ... 10 μl of overnight culture was dropped on charcoal PD plates for filament induction I and /or detection of GFP signal by ChemiDoc™ MP Imaging System (Bio-Rad, CA, USA). For the T7 endonuclease I assay ...
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bioRxiv - Bioengineering 2021Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 4°C during the LC-MS measurement ...
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bioRxiv - Biochemistry 2022Quote: ... 10μL of each time point were mixed with RNA loading dye and heated to 95°C before loading on a 1.0 cm well PAGE casting plate (Bio-Rad Laboratories, Mississauga, ON, Canada). Samples were run on a 7.5% Urea PAGE gel for 30 minutes at 300V in 0.5X TBE buffer ...
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bioRxiv - Plant Biology 2021Quote: ... were placed on 0.8% water-agar plates and transformed using biolistic bombardment with the PDS-1000/He™ system (Bio-Rad, Hercules, California, USA) as described above ...