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Citations for Bio-Rad :
2401 - 2450 of 6580 citations for Progesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Transfection took place over two rounds of electroporation at 850 V and 25 μF with an interval of 5 secs (Bio-Rad Gene Pulser Xcell Electroporation Systems) ...
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bioRxiv - Developmental Biology 2022Quote: ... The suspended eggs and siRNA (5 μg/μl stock solution, in MilliQ) were transferred to a 4 mm cuvette (Bio-Rad) with a final volume of 200 μl and were gently mixed ...
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bioRxiv - Neuroscience 2019Quote: ... and then eluted by boiling in SDS lysis buffer for 5 minutes at 95°C and resolved by SDS-PAGE (Bio-rad) gel ...
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bioRxiv - Microbiology 2019Quote: ... The lysates were boiled for 5 min in 1X XT sample buffer containing 1X XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT Precast 26-well gels (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... 80 μL of the supernatant was removed (termed ‘whole cell extract [WCE] sample’) and 5 μg of antibody (anti-PK (Bio-Rad) or anti-HA (Roche) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 150mM DTT was then added to each sample followed by the pelleting of membrane debris and undigested genomic DNA at 21000 x g for 5 min before the supernatant were separated on a Mini-PROTEAN TGX protein gel (Biorad, USA). Total protein was detected in gel by SYPRO Ruby Protein Gel Stain (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 150mM DTT was then added to each sample followed by the pelleting of membrane debris and undigested genomic DNA at 21000 x g for 5 min before the supernatant were separated on a Mini-PROTEAN TGX gel (Biorad, USA). PageRuler Prestained Protein Ladder (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... or T1L/T3D M2 μ1 M258I σ3 S344P virions or ISVPs (2×1012 particles/ml) were incubated for 5 min at the indicated temperatures in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl in virus storage buffer (10 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative RT-PCR (qRT-PCR) reactions were performed in 10 μL volumes using 5 μL of SsoAdvanced Universal SYBR Green Supermix (Bio-Rad), 4 μL of a mix containing 0.5 μM of each transcript-specific primer (Table S2) ...
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bioRxiv - Immunology 2019Quote: ... NK cells were pre-incubated with activating IB4 antibody (IgG2a, gift from Dr. Fabio Malavasi, University of Torino) blocking CD38 (clone AT13/5, Bio-Rad), CD31 (clone WM59 ...
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bioRxiv - Bioengineering 2019Quote: ... The fractions were analyzed by SDS-Page and fractions containing the protein of interest were pooled and loaded onto a 5 mL Mini-profinity IMAC column (Bio-Rad). Metal affinity chromatography was performed as previously described for the TEV protease ...
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bioRxiv - Microbiology 2019Quote: ... The gel was stained with 5 μg/ml ethidium bromide for 20 min and imaged using a Chemidoc XRS+ (Bio-Rad).
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bioRxiv - Biochemistry 2019Quote: ... 1.2 μL aliquots at various time points were quenched in 2X SDS-PAGE loading buffer (5 μL) and electrophoresed on 4 – 20 % TGX SDS-PAGE gels (Bio-Rad). Gels were imaged on a Typhoon variable mode scanner (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were run in triplicate in a Bio-Rad CFX96 Touch C1000 qPCR machine in 10 μl final volumes with 5 μl iTaq Universal SYBR Green Supermix (Bio-Rad), 0.5 μl of each primer and 1 μl DNA extract ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked 1 hr at room temperature with Odyssey PBS Blocking Buffer (Li-Cor, 927-40000) or in 5% Blotting-Grade Blocker (Biorad, 1706404) in TBST (50 mM Tris buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, USA). Alternatively ...
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bioRxiv - Molecular Biology 2020Quote: ... The blots were washed three times with TBS-T for 5 min each time before to development with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging on the ChemiDoc XRS+ System (Bio-Rad).
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... The incubation was terminated by boiling the samples at 95°C for 5 minutes in 1X Laemmli sample buffer (Bio-Rad) supplemented with 10% β-mercaptoethanol (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated with HRP-conjugated secondary antibodies (rat (IgM)/LM20 and LM19 mouse (IgG)/2F4 (in 5% milk PBS) and after washing chemiluminescence induced with Clarity Western ECL Substrate (Biorad, #1705061) and detected with ChemiDoc imaging system (Biorad).
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bioRxiv - Microbiology 2021Quote: ... Binding reactions were incubated for 40 minutes at 37 °C then run on a precast 5% TBE acrylamide gel at 4 °C for 45 minutes at 100 V (Bio-Rad). After transfer to Biodyne B Modified Nylon Membrane (ThermoFisher) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The samples were run with 5 mM H2SO4 at 0.6 mL/min on an Aminex HPX-87H (Biorad Inc., CA, USA) column at 50°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The second-dimension gel electrophoresis was performed in 1× TBE buffer containing 0.3 μg/ml of ethidium bromide at 6.0 V/cm for 5 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). DNA was transferred to Hybond-XL (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... Cell debris was cleared by centrifugation at 21000 g for 5 min at 4°C and supernatant was mixed with one-third volume 4X Laemmli Sample Buffer (Bio-Rad). For lysozyme lysis ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed four times in PBST for 5 minutes at room temperature with agitation and imaged directly using a ChemiDoc MP imaging system (Bio-Rad). Alternatively ...
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bioRxiv - Microbiology 2021Quote: ... proteins were denaturized in SDS-PAGE protein loading buffer for 5 minutes at 95°C and resolved by SDS-PAGE on 4-12% bis-tris Criterion XT Precast gels (Bio-rad), transferred to a PVDF membrane and blocked for at least 1h in 1% Casein blocking buffer (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of each protein sample was denatured and reduced by boiling at 95°C for 5 min in 10% (v/v) 2-mercaptoethanol-containing Laemmli sample buffer (Bio-Rad). Samples were resolved by SDS-PAGE with a precast Tris-glycine gel (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were subsequently blocked with 1x TBS containing 0.1% Tween 20 (1x TBST) with 5% (w/v) blotting-grade blocker (Bio-Rad, 1706404) and probed with the primary antibody of interest diluted in 1x TBS containing 0.1% Tween 20 and 0.5% blocker at room temperature overnight ...
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bioRxiv - Immunology 2021Quote: ... P2 was dialyzed against 1% glycine containing 2% ampholytes pH 3-10 (first run) or pH 5-7 (second run) and then loaded onto a liquid isoelectric focusing system (Rotofor, Bio-Rad). Individual fractions were harvested and their pH and absorbance at 280nm were measured ...
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bioRxiv - Immunology 2021Quote: ... pooled and subjected to two consecutive preparative isoelectric focusing (IEF) at pH 3-10 (first separation) and pH 5-7 (second separation) using a Rotofor Cell (Bio-Rad). The individual IEF fractions were harvested ...
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bioRxiv - Microbiology 2021Quote: ... About 0.5 μg of plasmid DNA were mixed with the cell suspension and transferred to ice-cold 1mm gap cuvettes (Bio-Rad). Cells were electroporated in a Bio-Rad Gene Pulser Xcell system using settings ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Cancer Biology 2020Quote: ... The beads were washed five times with IP lysis buffer and were boiled for 5-10 min in 2 times Laemmli sample buffer (#1610737, Bio-Rad) with 2-mercaptoethanol (#BP176-100 ...
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bioRxiv - Plant Biology 2019Quote: ... The primary antibody was detected with an HRP secondary antibody in 2.5% milk in TBST buffer at room temperature and chemiluminescence was recorded with the ChemiDocTM MP Imaging System from Bio-Rad.
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bioRxiv - Bioengineering 2020Quote: ... followed by 40 cycles of 95°C for 5 s and 60°C for 30 s (Bio-Rad CFX manager 3.1). Quantification of mRNA levels of different genes was performed using the 2−ΔΔct method ...
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bioRxiv - Genetics 2020Quote: ... membranes were washed in × 1 Tris-buffered saline-Tween (TSBT) and then blocked for ~10 min in 5% blocking buffer (Bio-Rad) in 1 × TBST ...
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bioRxiv - Cell Biology 2021Quote: ... against the OAD buffer for 6h at 4 °C to remove the sucrose and then loaded on an EnrichQ 5/50 column (Bio-Rad) equilibrated with the ion-exchange buffer A (50 mM HEPES pH 7.4 ...
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bioRxiv - Physiology 2021Quote: ... cDNA was diluted 1:5 and then 2μl was added to 5ul SYBR green PCR mixture (BioRad, 1725271, Hercules, CA, USA), 2.4ul water and 1.25 pmol primer mix ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were washed in PBS-T for three times 5 min and developed through chemiluminescence using a ChemiDoc XRS (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... and the sample was heated to 90°C for 5 minutes before loading of 3 pmol onto a 4-15% Mini-PROTEAN TGX Stain-Free Precast Gel (Bio-Rad), alongside PageRuler Prestained Protein Ladder (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... or 400 ng of total RNA was reverse transcribed to cDNA in a 20 µL reverse transcription reaction using 5 µL of iScript reverse transcription supermix (1708841, Bio-Rad Laboratories - Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed four times in PBST for 5 minutes at room temperature and imaged directly using a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed 3x for 5 min each in TBS without Tween and incubated with goat anti-mouse IgG coupled to horseradish peroxidase (BioRad, #170656) at a 1:3000 dilution in blocking buffer for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were incubated with Pierce Super Signal or Millipore Immobilon enhanced chemiluminescence reagents for 5 min and visualised using a ChemiDoc system (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Equal masses of protein (5 ug – 40 ug) were separated by 4—15% of SDS/ PAGE and were transferred onto nitrocellulose membranes (Bio-Rad) for protein blot analysis ...
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bioRxiv - Genetics 2021Quote: ... Samples were denatured at 100°C for 10 min and 5% of samples were loaded and run on a 10% Mini-Protean TGX gel (Bio-Rad). Electrophoresed proteins were transferred onto a nitrocellulose membrane using Turbo-blot Transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... The gel was stained with 5 μg/ml ethidium bromide for 20 min and imaged using the ChemiDoc XRS+ system (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: ... 10 µg of protein per sample were boiled for 5 min and size separated by electrophoresis in polyacrylamide gels (Bio-Rad). Proteins were transferred over-night at 4 °C to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... in TBST/5% wt/vol non-fat milk (1 h, RT), washed (5x, TBST) followed by addition of ECL substrate (Bio-Rad) and imaged (ChemiDoc ...
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bioRxiv - Immunology 2020Quote: ... in reducing or nonreducing conditions using SuperSep Ace 5%–20% gradient polyacrylamide gel (FUJIFILM Wako Pure Chemical, Osaka, Japan) and 2 × Laemmli Sample Buffer (Bio-Rad).
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.