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Citations for Bio-Rad :
2101 - 2150 of 2973 citations for CBP p300 IN 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and incubated with horseradish peroxidase-conjugated secondary IgGs (1:1,000 for rabbit #170-6515, mouse #170-6516, Bio-Rad, CA or 1:2,000 for #7076s ...
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bioRxiv - Synthetic Biology 2024Quote: ... Signal intensities were quantified using the Quantity One 1-D Analysis Software (Version 4.6.6) (Bio-Rad, Hercules, CA, USA). sRNA signal intensities were normalized to the corresponding 5S rRNA signals.
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using an iScript reverse transcriptase cDNA synthesis kit (Bio-Rad) to amplify all genes ...
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bioRxiv - Neuroscience 2024Quote: ... then 1 ml of “retentate” and “filtrate” were dot-blotted onto a nitrocellulose membrane (Bio-Rad Labs, Hercules, CA). Dot blots in which biotinylated Lec-bs was detected were performed as described below ...
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bioRxiv - Neuroscience 2021Quote: ... Each sample was prepared for western blotting by diluting 20 μg of protein with 1 × Laemmli buffer (Bio-Rad #1610747) and ultrapure water (Invitrogen #10977015 ...
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bioRxiv - Biophysics 2020Quote: The prepared DNA samples (5 μL) were mixed thoroughly with 1 μL of 6X DNA loading buffer (Bio-Rad Laboratories). The mixtures were loaded into the wells of the prepared gel ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined at indicated time points by adding 1/10 volume AlamarBlue solution (Biorad, Hercules, CA, United States) to each well ...
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bioRxiv - Cell Biology 2020Quote: ... and equal amounts (1–2µg) were used for reverse transcriptase reaction using an iScript™ Reverse Transcription Supermix (Bio-Rad). qPCR was run using the SYBR® Green Master Mix on a CFX Connect™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were co-encapsulated with Drop-Seq beads (ChemGenes: lot 083117) in 1 nL droplets using 15000 mL/hr oil (QX200, Biorad), 4000 mL/hr cell suspension and 4000 mL/hr bead flow rates (droplet generation frequency 2 kHz) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amount of proteins was loaded onto 12% SDS-PAGE gel (acrylamide: bisacrylamide 29:1, cat# 161-0146, Bio-Rad) and after size separation blotted onto 0.2 μm nitrocellulose membrane (cat# 162-0112 ...
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bioRxiv - Cell Biology 2020Quote: ... incubating with HRP conjugated secondary antibodies (1:5000) and developing with Clarity™ Western ECL blotting substrate (Bio-Rad, 1705061) and imaging with X-ray film ...
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bioRxiv - Genetics 2021Quote: ... Proteins were visualized using horseradish peroxidase-conjugated goat anti-rabbit secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706515) or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... and switch time linearly ramped from 1 s to 5 s over 14-15 h (CHEF DR III, Bio-Rad). DNA bands were visualized by staining the gel with a solution of 0.5 µg/mL ethidium bromide ...
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bioRxiv - Developmental Biology 2022Quote: ... membranes were incubated with ECL Western Blotting Substrate (Thermo, 32106) for 1 minute prior to imaging with the ChemiDoc system (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was used for reverse transcription with iScript™ cDNA synthesis kit (Bio-Rad, Hercules, CA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of plasmid was added to electrocompetent GBS cells and transferred to a cold 1 mm cuvette (Fisher or BioRad). Electroporation was carried out at 2.5 kV on an Eppendorf eporator ...
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bioRxiv - Neuroscience 2021Quote: ... A 1:5 dilution from serum samples was combined with 4 °C Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µg of cleaned RNA was used to generate cDNA with iScript reverse transcriptase per the manufacturer’s protocol (Bio-Rad). 20 µL quantitative real-time PCR reactions with 100 ng of cDNA input were carried out using TaqMan Fast Advance Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-IBA-1 antibody (0.4 μg/mL, FUJIFILM Wako) and rat anti-CD68 antibody (5 μg/mL, clone FA-11, Bio-Rad) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA with random primers using an iScript cDNA synthesis kit (Bio-Rad) and analyzed with a Bio-Rad CFX96 real time system ...
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bioRxiv - Immunology 2022Quote: ... 1-0.2 μg of RNA was used to synthesize cDNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA). The cDNA template was mixed with iTAQ SYBR green universal super mix (Bio-Rad) ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were rinsed several times in 1xTBSTw and incubated with goat anti-rabbit HRP-conjugated secondary antibody (1:20,000; Bio-Rad, catalog 179-5046 ...
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bioRxiv - Molecular Biology 2019Quote: ... and goat anti-rabbit IgG H&L HRP conjugate at a dilution of 1:2000 (Bio-Rad, Hercules, CA, USA) was used as the secondary antibody ...
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bioRxiv - Cell Biology 2019Quote: ... the blots were incubated with a secondary antibody conjugated to horseradish peroxidase (HRP) (1:5,000, Jackson Immuno Research Laboratories) and detected with a ChemiDoc XRS system (Bio-Rad). Primary antibodies used in western blot are listed below ...
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Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosisbioRxiv - Evolutionary Biology 2019Quote: ... immunoprecipitated chromatin and a 1:100 dilution of the input chromatin were separately recovered by boiling with a 10% Chelex-100 resin (BioRad) before treating with 25 μg/ml Proteinase K at 55 °C for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... membrane was washed repeatedly with PBS-T and incubated 1 h RT with the secondary antibody in milk PBS-T (Goat anti-mouse IgG HRP conjugate (170-6516; BioRad); Goat anti-rabbit IgG HRP conjugate (170-6515 ...
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bioRxiv - Pathology 2019Quote: ... Bands corresponding to EPCR and the housekeeping protein β-actin (mouse IgG #NB600-501, Novus, at 1:40,000) were detected by the chemiluminescence method (Clarity Western ECL Bio-Rad) and finally densitometrically measured by ImageJ 1.6.0 software.
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bioRxiv - Cell Biology 2019Quote: ... The secondary antibodies used for Western blotting were goat ⍰ -mouse IgG HRP conjugate (170-6516 from Bio-Rad; 1:2000) and goat ⍰ -rabbit IgG HRP conjugate (170-6515 from Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... according to the manufacture’s protocol and 1 µg RNA was used to convert to cDNA using iScript Select cDNA Synthesis Kit (Bio-Rad) in the presence of both oligo (dT ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated with NaIO4 for 1 h then 15 min with NaS2O3 and mixed with 100 μl AffiGel Hz Hydrazide Gel (Biorad) overnight ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse transcription of 1 μg of total RNA per sample was performed using the iScript cDNA synthesis kit (Bio-RAD). Transcript levels were determined by quantitative real-time RT-PCR as previously reported [14] using primers provided in Supplementary Table 1 and normalizing expression to ATCB transcript levels.
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bioRxiv - Microbiology 2020Quote: ... The gel was run for 1 h at 100 V and visualized on a ChemiDoc MP Imaging System (BioRad, Germany).
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bioRxiv - Neuroscience 2019Quote: ... Each qPCR reaction was performed in triplicate using specific primers (Supplementary Table 1) and iQ SYBR Green Supermix (Bio-Rad) in a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2019Quote: ... Primary antibodies used for immunolabelling (Table 1) were utilized in combination with appropriate secondary antibodies purchased from BioRad (München, Germany) and BCIP-NBT (VWR international ...
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bioRxiv - Synthetic Biology 2019Quote: ... Pulsed-field electrophoresis was carried out using a 1% agarose gel in 0.5x TBE buffer on the CHEF-DR III system (Bio-Rad). The entire 10 µL of each above described reaction was mixed with 10 µL of molten (55°C ...
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bioRxiv - Microbiology 2019Quote: ... The nitrocellulose membrane was washed three times with 1x TTBS and then probed at a 1:20,000 dilution of HRP-conjugated goat α-rabbit antibody (Biorad) in 5% non-fat milk solution for one hour ...
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bioRxiv - Bioengineering 2019Quote: ... The cell suspension is then embedded in 1% low-melt agarose plugs and treated using the CHEF Mammalian Genomic DNA Plug Kit (Biorad), according to the manufacturer’s protocol38,39 ...
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bioRxiv - Cancer Biology 2020Quote: ... ran at 120 V for ∼2 h and transferred at 100 V for 1 h onto nitrocellulose membranes (Bio-rad) using the wet blot method ...
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bioRxiv - Cell Biology 2019Quote: ... The labeled HA disaccharides were electrophoresed in a Bio-Rad mini-PROTEAN Tetra system using a gel composition of 20% acrylamide (37.5:1; Bio-Rad), 40 mM Tris acetate (pH 7.0) ...
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bioRxiv - Immunology 2021Quote: ... serially diluted rabbit serum (5-fold dilution starting with 1:80) were tested for binding to color-coded beads by Bio-plex (Biorad). The beads were coated with avi tagged C.1086 WT ...
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bioRxiv - Plant Biology 2020Quote: ... was used at a dilution of 1:2,000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:2,000) followed by a chemiluminescence reaction (Clarity Western ECL Substrate; Bio-rad). For the SS-TOM lines analysis ...
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bioRxiv - Neuroscience 2020Quote: ... RNA (1 μg) was reverse-transcribed to cDNA with the iScript ™ cDNA Synthesis Kit (Bio-Rad Laboratories, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Gel was stained for 1 h with 0.1 μg/ml ethidium bromide and visualized by ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (1: 8000; Cat. 170-6516, Bio-Rad, Milan, Italy) was used ...
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bioRxiv - Cell Biology 2020Quote: ... Detergent was removed by three addition of BioBeads SM-2 adsorbent resin (25 mg per 1 mL of lipid mixture) (BioRad) for 2 h each at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and eIF2B4 loci were amplified with the primer pairs detailed in Table 4 and run on a 1% agarose gel and imaged using a ChemiDoc XRS+ imaging system (Biorad). The expected WT fragment length for the eIF2B1 ...
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bioRxiv - Cell Biology 2021Quote: ... 15% for FKB-2) and transferred onto nitrocellulose membranes for 1 hour at 200 mA (SemiDry transfer blot, Bio-Rad). After incubation with blocking solution (LICOR Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was diluted 1:100 and real-time PCR was performed in triplicate with iQ SYBR Green Supermix from BioRad.
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was synthesized from 1 μg of isolated RNA using iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA). RT-qPCR was carried out using SensiMix™ SYBR® and Fluorescein kit (QT615-05 ...