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Citations for Bio-Rad :
1951 - 2000 of 2973 citations for CBP p300 IN 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... Reverse transcription reactions were performed from 1 μg RNA in 20 μL reactions using iScript reverse transcriptase (Bio-Rad). qPCR reactions were prepared in triplicate in a final volume of 10 μL using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Immunology 2019Quote: Samples were diluted 1 in 3 in HBSPE and run through Bio-Gel® P-30 (Bio-Rad, UK) polyacrylamide gel spin columns to minimise non-specific binding ...
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bioRxiv - Synthetic Biology 2019Quote: ... Biorad) was applied for 1 h at RT and blots were developed with Carity™ Western ECL Substrate (Biorad).
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bioRxiv - Genetics 2020Quote: ... 2 mM ATP and 1 mM DTT and the reactions were stopped by addition of Laemli sample buffer (BioRad). Samples were run on precast 4-20% gradient SDS-PAGE gels (BioRad ...
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bioRxiv - Developmental Biology 2020Quote: Antibodies used in this study are specific to sheep GFP (1:500; AbD Serotec, Bio-Rad, Hercules, CA, USA), chick GFP (1:500 ...
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bioRxiv - Genomics 2021Quote: ... PFGE was performed using 1% agarose gel in 0.5X TBE (Thermo-Fisher) in a CHEF Master XA 170-3670 system (BioRad) with the following parameters ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 1 mM DTT) and transferred onto a nitrocellulose membrane using the Transblot Turbo Transfer System (Biorad, 1704150EDU). Non-specific binding sites were blocked for 1 hour with 5% dry milk or BSA in TBS-Tween 0.1% while the primary antibody (1/1000 in 1% BSA TBS-Tween 0.1% ...
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bioRxiv - Cell Biology 2021Quote: ... RNA (1 μg) was reverse transcribed into cDNA using iScript Reverse Transcription Supermix Kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared to the control gene 18S were determined by quantitative real-time PCR using SYBER Green PCR Supermix (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: Equivalent number of T cells (1×10e6) were lysed in Laemmli Sample Buffer with β-mercaptoethanol (Bio-Rad, CA). Total cell lysate for each sample were resolved on 12% SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 ug) was converted to cDNA using the iScriptTM cDNA synthesis kit (# 1708891, BIO-RAD, Hercules, CA) following the manufacturer’s protocol (BIO-RAD ...
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bioRxiv - Bioengineering 2021Quote: ... Amplifications were performed in a 20 μL reaction containing 1 × ddPCR Supermix for Probes (No dUTP),(Bio-Rad, 1863024), 250 nM of probes and 900 nM of primers and 8 μL EV DNA template ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were developed with a peroxidase-labeled goat anti-mouse or anti-rabbit secondary antibody (1:5,000, Biorad) and chemiluminiscence detection (MF-ChemiBIS 3.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates prepared at a concentration of 1 mg/ml and supplemented with 4x Laemmli Sample Buffer (Bio-Rad: 1610747) supplemented with fresh 2-mercaptoethanol (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and exposed to X-ray film (Kodak) or the ChemiDoc Imaging system (Biorad) after incubation with Thermo Scientific Pierce ECL or Clarity ECL (Biorad).
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bioRxiv - Physiology 2021Quote: ... membranes were rinsed three-times in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit or anti-mouse IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Biochemistry 2021Quote: Reaction products were analysed by 20% denaturing polyacrylamide gel electrophoresis (made from 40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) and 7 M urea (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... samples were combined and run on a 20% denaturing polyacrylamide gel (40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) and 7 M urea (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-syntenin-1 (SCBT, sc-515538) and horseradish peroxidase-conjugated secondary goat anti-mouse (Bio-Rad, 170-6516), goat anti-rabbit (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... at a 1:10,000 dilution and we developed blots using the AP conjugate substrate kit (Bio-Rad; Hercules, USA). We used polyclonal GFP antibodies (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:5000 ...
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bioRxiv - Plant Biology 2021Quote: ... Four- to six-week-old expanded leaves of relevant Arabidopsis lines were bombarded with 1 nm gold particles (BioRad) coated with pB7WG2.0-mRFP ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were detected using anti-mouse IgG horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3,000; catalog number NEF822001EA, Perkin-Elmer) and developed with Clarity Western ECL Substrates (BioRad). All antibodies were diluted in TBS-T with 1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... Chromosomes were separated on a 1% pulse-field certified agarose gel on the CHEF Mapper XA system (Bio-Rad) under the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... cDNA samples were diluted 1:15 and used for qPCR with the iTaq Universal SYBR green mastermix (Biorad, #1725120). Cycle threshold values were corrected for PCR efficiency of each primer set and normalized to the hypoxanthine phosphoribosyltransferase 1 (HPRT ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit monoclonal antibody specific for Aβ42 (1:2,000, BioLegend 812101. Secondary antibodies were fluorescently labelled. Fluorescence was detected on a ChemiDoc MP system (Biorad). Immunohistochemistry was carried out as described (44) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 mg of proteins were diluted in ChIP buffer supplemented with 0.01% SDS and precleared 1 h at 4 °C with 50 μl of protein A agarose beads (BioRad) and 100 μg BSA ...
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bioRxiv - Cell Biology 2021Quote: ... This was followed by incubation with a secondary HRP-conjugated anti-rabbit or anti-mouse antibody (1:2000, BioRad), depending on the origin of the primary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed 4 times with TBST and incubated with secondary antibody (1:2000, HRP-conjugated anti rabbit, BioRad) for 1hr at room temperature then washed 4 times with TBST ...
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bioRxiv - Developmental Biology 2022Quote: ... We used 1 μg of RNA to produce cDNA with iScript™ Reverse Transcription Supermix (Bio-Rad, Cat#1725120). The cDNA was subjected to quantitative real-time PCR with iTaq™ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2022Quote: ... small RNAs were isolated (18nt–30nt) from 1 μg of total RNA using 15% urea-PAGE (Bio-Rad, USA) followed by overnight precipitation using 0.3% NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradient was fractionated using a Gradient Station (BioComp) coupled with an ultraviolet 254nm detector (Bio-Rad EM-1).
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bioRxiv - Microbiology 2022Quote: ... the samples were washed extensively with lysis buffer and the proteins were eluted by boiling 5 ul of the resin with 5x SDS loading buffer and the proteins were detected by western blot analysis using rabbit antibody to FLAG (1:2,000, BioRad) or mouse antibody to Myc (1:2000 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL protein was injected onto either a self-packed buffer exchange column (P6 polyacrylamide gel, Bio-Rad Laboratories) or a prototype desalting column from Thermo Fisher Scientific and online buffer exchanged to 200 mM ammonium acetate ...
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bioRxiv - Molecular Biology 2022Quote: ... were used at a concentration of 1:2,000 and signals were visualised using Clarity Western ECL substrate (Bio-Rad) on a ChemiDoc MP (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of each RNA sample was reverse transcribed to cDNA using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR was performed on cDNA samples using the iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: All analytical gels were performed in 20% polyacrylamide gels (acrylamide:bisacrylamide 19:1) using 8×12 cm plates in a mini-PROTEAN system (BioRad). Acrylamide solution was prepared in 1X TBE buffer (89 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... the tubes were dialyzed twice for 48-72h against buffer I (25mM Tris pH 7.4, 25 mM NaCl, 1 mM β-mercaptoethanol and 0.5 g of Bio-Beads (Biorad). After dialysis ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was incubated at 4°C for 1 h with Bio-Beads™ SM-2 resin (Bio-Rad) equilibrated with 100 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of each cDNA sample was amplified with gene-specific primers in iQ SYBR Green Supermix (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed two times with TBST and then incubated with a 1:5,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed with TBST and then incubated with a 1:10,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Bioengineering 2021Quote: ... A sample containing 1-20µg of protein was loaded into a 4-15% precast gel (Bio-Rad Mini-ProTEAN) and run at 60V for 20min followed by 160V for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... Membranes were washed and probed with secondary antibody (1:5000, anti-Rabbit-HRP, 1706515; anti-Mouse-HRP 1706516, BioRad) in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... The master mix for droplet digital PCR (ddPCR) amplification included 1× ddPCR Supermix for probes (no dUTP, Bio-Rad), 0.2 1M of each primer and 0.2 1M probe (both IDT ...
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bioRxiv - Microbiology 2022Quote: ... we used 10µL of desalted assembly reaction products to transform competent E.coli S17-1 cells (mid-log cells, washed three times in deionized ice-cold water) by electroporation (V=1.8kV; MicroPulser, BioRad). After 1h recovery at 37°C in 1mL of LB ...
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bioRxiv - Pathology 2022Quote: ... The expression levels of target proteins were quantified with Quantity One 1-D Analysis Software (Bio-Rad, Hercules, CA) and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Zoology 2019Quote: ... and specific primers (Table 1) on a CFX96 Touch™ Real-Time PCR Detection System (Biorad, Hercules, CA, USA). Actin was used as housekeeping gene normalization control (Table 1) ...
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bioRxiv - Microbiology 2019Quote: ... Blots were incubated with appropriate horseradish peroxidase -conjugated secondary antibody goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... Cells were then stained for inflammasome activation using a caspase-1 FAM-YVAD-FMK FLICA probe kit (Bio-Rad). Cells were then processed for analysis by flow cytometry.