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Citations for Bio-Rad :
2001 - 2050 of 2973 citations for CBP p300 IN 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription was performed on 1 μg of purified RNA using the iScript Reverse Transcription Supermix kit (Bio-Rad). The cDNA was then added to SsoFast (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then pelleted in a 1.5 mL tube and resuspended in 1 mL phosphate buffered saline pH 7.8 (Bio-Rad) (PBS) ...
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bioRxiv - Cell Biology 2019Quote: ... Target transcripts were amplified using primers listed in Table 1 and Sso Advanced Universal SYBR Green Supermix according to manufacturer’s protocol (BioRad). All qPCR reactions were assayed in triplicate for each sample and the average Cq value was used to calculate the mean expression ratio of the test sample compared to the control sample (i.e ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription of 1 μg RNA/20μl was performed with the iScript™ cDNA Synthesis Kit (1708890, Bio-Rad). qPCR was performed with iTaq™ Universal SYBR® Green Supermix (1725120 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were washed 3 times with PBS and then blocked for 1 hour at room temperature with DPBS with 0.4% Triton X-100 and 5% non-fat dry milk (BioRad). Cells were stained with primary and secondary antibodies (Table S3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated with respective anti-mouse or anti-rabbit HRP-conjugated secondary antibody (1:1000, Bio-Rad) and visualized with a chemoluminescence kit (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: Serum cytokines were quantified using the Bio-plex Pro Mouse Cytokine group 1 panel 8-plex kit (Biorad M60000007A). Samples were treated according to the manufacturer’s instructions and acquired on the Bio-Plex® 200 system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... which was inoculated at 30°C for 1 hour and then photographed using a UV transilluminator (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... Amplification products were resolved in 1% (w/v) agarose gel and analysed with GelDoc XR+ System (Bio-Rad Laboratories). Primers and probes are listed in Table S1.
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were detected with anti-mouse HRP-conjugated secondary antibodies (1:5000; Bio-Rad, Hercules, CA, USA), and the signal was developed using Supersignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used: rabbit anti-mouse collagen-IV (Bio-Rad Company, cat.# 2150-1470, 1:300), rat anti-mouse CD45 (BD Pharmingen ...
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bioRxiv - Genomics 2019Quote: ... All blots were visualized using a goat anti-rabbit IgG:alkaline phosphatase fusion protein at 1:3,000 dilution in conjunction with BCIP/NBT color development (Bio-Rad).
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bioRxiv - Neuroscience 2019Quote: ... First-strand cDNA synthesis was performed from 1 μg isolated RNA template using iScript RT supermix (Bio-Rad #1708841). PCR products were amplified using a CFX96 Real-Time PCR System (Bio-Rad) ...
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bioRxiv - Biophysics 2019Quote: ... Gel precursor solutions were prepared with 6% v/v acrylamide and 0.2% v/v bisacrylamide (30% acrylamide/bisacrylamide stock 29:1, BioRad), 2 mM LAP photoinitiator (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... RNA (1 μg) was reverse transcribed in 20 μl reactions using the BioRad iScript cDNA Synthesis Kit (BioRad Laboratories) with 1:1 mixture of oligo-dT and random hexamers for 30 min at 42°C ...
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bioRxiv - Genomics 2021Quote: ... 1 μL diluted cDNA in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each sample we conducted three technical replicates and used their mean as the data point ...
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bioRxiv - Microbiology 2020Quote: ... 15 % methanol (v/v) in distilled water) for 1 hour at 100 volts (Mini Trans-Blot Cell, Bio-Rad). The nitrocellulose membrane was blocked in blocking buffer (5% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Complementary DNA was synthesized from 1 μg total RNA with an iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). A CFX 96 Connect™ system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the Amylose resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were washed in TBS-Tween and incubated with the peroxidase-conjugated secondary antibody (Bio-Rad, Table 1) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were stained overnight at 4°C with a 1:1000 diluted mouse anti-his primary antibody (MCA1396, RRID:AB_322084, Bio-Rad) and then for 1 hour at room temperature with a 1:4000 diluted rabbit anti-mouse HRP secondary antibody (SouthernBiotech Cat# 6170-05 ...
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bioRxiv - Molecular Biology 2021Quote: ... the Netherlands) and blocked for 1 hour at room temperature using Odyssey blocking buffer (LI-COR biosciences, Bio-Rad) in a 1:1 dilution with tris-buffered saline (TBS ...
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bioRxiv - Genomics 2020Quote: ... 1 ug of mRNA was reverse-transcribed into cDNA using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA). RNA was hydrolyzed and re-suspended in nuclease free water ...
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bioRxiv - Immunology 2020Quote: ... We used FITC-conjugated mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:20) and Alexa Fluor 647-conjugated mouse anti-pig CD8α (clone 76-2-11 ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 minute and then imaging every 10 seconds for 5 minutes on the ChemiDoc XRS+ machine (Bio-Rad). ImageLab (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of purified RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad). Real-time PCR analysis was performed using the iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA (1 μg) was used to generate cDNA using the Biorad iscript RT supermix RT-qPCR kit (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and Goat anti-rabbit Igg (H+L) horseradish peroxidase-conjugated secondary antibody (Bio-Rad, 1:5000; Cat. 170-6515,) were used as secondary antibodies ...
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bioRxiv - Genomics 2022Quote: ... membranes were rinsed threetimes in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Plant Biology 2022Quote: ... USA (17) and a 1:3000 dilution of goat anti-rabbit IgG horseradish peroxidase (HRP) conjugate secondary antibody (Biorad). Chemiluminescence was detected using WesternBright ECL (Advansta ...
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bioRxiv - Microbiology 2022Quote: ... A 15% SDS-Tricine gel was made by mixing: 2.5 mL 30% acrylamide/Bis solution 37.5:1 (BioRad #1610158), 1.25 mL gel buffer (1.5 M Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was subsequently washed and incubated in HRP-conjugated secondary antibody against the appropriate host (1:10,000, BioRad) for 1 hour RT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... together with 2.5 µl of single (1 µl assay + 1.5 µl NF water) or multiplexed (0.5 µl of each assay) PrimePCR Probe Assays (Bio-Rad) as follows with single or multiplex reactions grouped in parentheses and formatted as Gene/UniqueAssayID ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 μ of total RNA using iScript gDNA clear cDNA synthesis kit (Bio-Rad, 1725035) with random primers ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed on 1 μg of RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad #1708891). Then ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli and plasmid DNA were electroporated in 1 mm cuvettes at 1.8 kV using the MicroPulser electroporator (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... We synthesized the first strand cDNA with 1 µg of total RNA using iScriptTM Reverse Transcription Supermix (BIO-RAD) followed by quantitative PCR with CFX96 Real-Time System (BIO-RAD ...
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bioRxiv - Cancer Biology 2024Quote: Cells were first embedded in gel plugs with approximately 1 million cells per gel plug (BioRad no. 170-3592). The high molecular weight genomic DNA was extracted and purified using the Bionano Genomics SP kit.
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bioRxiv - Cell Biology 2024Quote: Polyacrylamide gel electrophoresis was carried out using 10 % gels (acrylamide/bis-acrylamide ratio 37.5:1) or any kDa precast gels (Biorad) and a MiniProtean (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... Cell lysates and purified supernatants were separated on an SDS-PAGE and analyzed through immunoblotting against vinculin as a loading control (1:2,000, MGA465GA, BioRad) or CHIKV E1 glycoprotein (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of the RNA was reverse transcribed into cDNA with the iScript Advanced cDNA Synthesis kit (Bio-rad). 20 μL of the end products were diluted by adding 180 μL nuclease-free water and stored at -20 °C until further analysis.
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated goat anti-rabbit IgG (1:5000; 1706515, Bio-Rad) at RT for 1 h followed by chemiluminescent detection with ECL Substrate (1705060 ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription (RT) was performed on 1 µg RNA with the iScript™ Reverse Transcription Supermix (Bio-Rad 1708841) for 5 min at 25°C ...
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bioRxiv - Genomics 2024Quote: ... F43E2.6 siRNA and miR-1 Ct values were assayed using a CF96 Real-Time PCR Detection System (Bio-Rad). Ct values were averaged across three technical replicates for each of 3 biological replicates.
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bioRxiv - Plant Biology 2024Quote: ... cDNA was generated from 1 μg of RNA using the iScript Reverse Transcription Supermix for RT-qPCR kit (BioRad). PCR with Chr07:9757891 For and Chr07 9758683 Rev primers was performed using APEX master mix ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using candidate primers (Integrated DNA Technologies; Table 1) and an iScript cDNA Synthesis Kit (Bio-Rad). Relative mRNA expression was analyzed by quantitative RT-PCR using the QuantStudio PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... and goat anti-rabbit (Cat. # 170-6515) antibodies were used at 1:2000 for immunoblotting and purchased from Biorad.
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bioRxiv - Genomics 2024Quote: ... samples were embedded in a thin polyacrylamide film by inverting them onto a GelSlick-coated microscope slide with a droplet of 4% acrylamide solution (4% v/v 20:1 acrylamide:bis-acrylamide [BioRad, 1610144] with 0.15% v/v TEMED [Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...