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Citations for Bio-Rad :
2051 - 2100 of 2175 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and Reverse Transcription (RT) was performed with 2 μg RNA per 40 μl reaction mixture using iScript Reverse Transcription Supermix (Bio-Rad #170-8891). RT-qPCR was performed using primers antibodies (Key Resources Table) ...
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bioRxiv - Immunology 2020Quote: SARS-COV-2 RBD-SD1 gBlock (IDT) was amplified by polymerase chain reaction (PCR) with iProof High-Fidelity PCR system (Bio-Rad, Cat # 1725310) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... was used for quantification and all PCR reactions were prepared using the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad Cat# 1864022). Primers used were previously described in (25) ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real time polymerase chain reaction for all targets was performed on the CFX-96 Real Time System using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA). Products were confirmed by gel electrophoresis ...
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bioRxiv - Immunology 2020Quote: ... Reactions were performed in triplicates in a final reaction volume of 10 μl containing 5 μl of iQ™ SYBR® Green Supermix (Biorad, France), 4 μl of cDNA diluted 1:15 in DEPC-water and 0.5 μl of each forward and reverse primers at a final concentration of 0.5 μM (Table S2 ...
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bioRxiv - Immunology 2021Quote: ... cDNA (2.1 ng) served as template for a qPCR reaction using SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad Laboratories, Hercules, USA) and forward and reverse primers in a final concentration of 333 nM each ...
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bioRxiv - Genetics 2021Quote: ... Thirty nanograms of cDNA per reaction were analyzed for semi-qPCR using the SsoAdvanced™ Universal SYBR® Green Supermix Kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Target amplification within each droplet was conducted in the C1000 Touch™ Thermal Cycler with 96-Deep Well Reaction Module (Bio-Rad, 1851197) through the following PCR protocol ...
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bioRxiv - Neuroscience 2021Quote: ... was used from each sample for One-step reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) using the iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad) in the StepOnePlus™ Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Genomics 2021Quote: ... Partitioning of the reaction mixture into up to 20,000 droplets was carried out on a QX200 ddPCR droplet generator (Bio-Rad, Laboratories, Munich, Germany) and PCR was performed using a Mastercycler Pro (Eppendorf ...
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bioRxiv - Molecular Biology 2021Quote: PCR reactions were set up using the following volumes: 10 µL 2x ddPCR Supermix for probes (no dUTP) (BioRad Laboratories© 186-3024), 5 µL of 20plex oligo mix ...
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bioRxiv - Microbiology 2023Quote: ... were detected as dark spots after a 10-min reaction with 5-bromo-4-chloro-3-idolyl phosphate and nitro blue tetrazolium using an alkaline-phosphatase-conjugate substrate (Bio-Rad, CA, USA). SFUs were counted using the AID ELISpot Reader System (Autoimmun Diagnostika) ...
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bioRxiv - Molecular Biology 2023Quote: Relative genetic expression was determined using Real Time Quantitative Polymerase Chain Reaction (RT-qPCR) (CFX96™ Real-Time System, Bio-Rad, CA, USA) using commercially available TaqMan primers (Tables 1-3 ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and PCR amplification were performed in a single reaction using the One-Step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules, CA, USA) per the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Quantitative real time polymerase chain reaction (qPCR) was performed using the iQ™ SYBR® Green Supermix (170888, Bio-Rad, Hercules, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... to allow detection of both in one reaction droplet ddPCR was performed with a BioRad QX100 ddPCR machine using the recommended protocols and reagents (BioRad Labatories, Hercules, CA). ddPCR was performed on biological quadruplicates of males and females ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Neuroscience 2022Quote: ... PCR Reactions were then performed in triplicate (3n) using a Dye Based iTaq Universal SYBR® Green Supermix (Bio-Rad, Hercules California #1725121). The Thermal Cycling protocol was performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... We optimized the recommended polymerase chain reactions (PCR) cycling conditions and performed a touchdown PCR in a T100 thermocycler (Bio-Rad, Hercules, CA). The total reaction volume was 50 µl which contained 2 µl of genomic DNA at 50 ± 25 ng/µl ...
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bioRxiv - Microbiology 2024Quote: ... The copy number of specific transcripts was determined by analysing mouse cDNA in a 10 μl reaction mix consisting of 1x iQ SYBR® Green Supermix (Bio-Rad, USA), 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... We performed the reverse transcription (RT) reaction with 200 ng/µl of total RNA with the iScript gDNA clear advanced kit (Bio-Rad 172-5035) and the real time qPCR was performed with the Sso Advanced SYBR Green® Supermix (Bio-Rad 172-5270 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We incubated the reaction for 1 hour at 50°C in a thermal cycler (Bio-Rad C1000 Touch Thermal Cycler, Bio-Rad, USA).
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bioRxiv - Developmental Biology 2024Quote: ... and the reactions were stopped by boiling them at 95°C for 5 minutes after adding Laemmli sample buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol (Gibco 21985023) ...
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bioRxiv - Plant Biology 2023Quote: ... reactions were prepared in a 10 µL final volume containing 5 µL of 2x SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad), 0.125 µL of each specific primer pair at 250 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... All PCR and RT-PCR reactions were performed on a CFX96 TouchTM real-time PCR detection system (Bio-Rad Laboratories, Inc. Hercules, CA).
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bioRxiv - Bioengineering 2024Quote: ... at the CCRL2 locus.60 The ddPCR reaction was prepared and underwent droplet generation following the manufacturer’s instructions with a Bio-Rad QX200 ddPCR machine (Bio-Rad, Hercules, CA, USA). Thermocycler settings were as follows ...
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bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Quantitative RT-polymerase chain reaction (qRT-PCR) experiments were performed using the CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules) with PowerUP SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... Transcript abundance in cDNA dilutions of 1/100 was measured in 25 µl reactions using qPCRBIO SyGreen LO-ROX RT-qPCR reagent (PCR Biosystems) with relevant primers on a CFX96 qPCR machine (Bio-Rad, California, US). Transcript abundance was normalized to TaHK4 (TraesCS5A02G015600 ...
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bioRxiv - Cancer Biology 2023Quote: ddPCR assays performed on cfDNA and EV-RNA were from Bio-Rad Laboratories Inc (ERBB2: dHsaCP1000116; dHsaCPE5037554, EIF2C1: dHsaCP2500349, EEF2: dHsaCPE5050049) and PCR reactions were run on T1000 thermal cycler (Bio-Rad, Hercules, CA, USA). Analysis of ddPCR data was analyzed using QuantaSoft Software ...
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bioRxiv - Genetics 2023Quote: ... Co-IP reactions were rotated overnight at 4°C with 100 µl of SureBeads Protein G Magnetic Beads (BioRad; Catalog# 1614013; Hercules, CA). The bead lysates were washed five times in the co-IP buffer using a DynaMag Magnet (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Viral RNA genome was detected and quantified by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) on a BioRad real-time CFX96 detection system (BioRad, Hercules, United States). The target sequence for amplification was the viral RNA-dependent RNA polymerase [26].
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bioRxiv - Genomics 2024Quote: ... 10 ng of purified genomic DNA was added to a reaction consisting of 10 µL of ddPCR Supermix for Probes (Bio-Rad, Cat #1863024), 1 µL of MseI (New England Biolabs ...
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bioRxiv - Systems Biology 2024Quote: ... qPCR was performed in 20uL reaction volumes with KBTBD2 assay together with GAPDH and amplified using iTaq universal probes supermix (Bio-Rad Laboratories, #1725134). Relative quantification was performed using (2–ΔΔCt).
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bioRxiv - Immunology 2024Quote: ... Polymerase chain reaction (PCR) was carried out in 10 μL in triplicate using the iTaq Universal SYBR Green One-Step kit (Bio-Rad, Cat# 1725150) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized with oligo-dT primers and random hexamers using the iScript cDNA synthesis reaction kit (Bio-Rad, Catalog No. 170–8891) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2024Quote: ... Figure S1-S8) were measured using reverse transcription quantitative polymerase chain reaction (RT-qPCR) with Bio-Rad CFX96 (Bio-Rad, Hercules, CA, USA) in a reaction of 20 μ L ...
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bioRxiv - Biochemistry 2024Quote: ... The qPCR reactions with the cDNA and appropriate forward and reverse primers were set up using iTaq Universal SYBR® Green Supermix (Bio-Rad, 1725121) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... One microgram of RNA was reverse transcribed to complementary DNA in 20 μL reaction volumes using iScript Reverse Transcription Supermix (Bio-Rad, Cat. 1708841) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... PCR enriched fragments were further size-selected by running the PCR reaction mix in 2% low-molecular weight agarose gel (Bio-Rad, 161-3107) and subsequently purifying them using QIAquick Gel Extraction Kit (28704) ...
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bioRxiv - Biochemistry 2024Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with 30 ng of cDNA using the CFX Connect qPCR system (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... PCR enriched fragments were further size-selected by running the PCR reaction mix in 2% low-molecular weight agarose gel (Bio-Rad, 161-3107) and subsequently purifying them using QIAquick Gel Extraction Kit (28704) ...
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bioRxiv - Developmental Biology 2024Quote: ... qRT-PCR was performed in a 10 μl reaction system with iTaq™ Universal SYBR® Green Supermix (Bio-Rad, Cat# 172-5121). Each genotype and developmental time point had three technical replicates ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were treated with ECL chemiluminescence detection reagent (GE healthcare, product # RPN2106V1 and RPN2106V20 and imaged using the Bio-Rad Chemidoc system (Bio-Rad Laboratories). Densitometry was done using ImageLab software (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were run in a 2% agarose slab gel and stained with ethidium bromide for visualization by UV shadowing (Bio-Rad Molecular Imager Gel Doc XR+).
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bioRxiv - Microbiology 2021Quote: ... The bound dye in the wells was eluted with 33% acetic acid and the absorbance of the solution was read at 595nm in a microtitre plate reader (BioRad i-Mark, Japan). To assess the individual bacterial population during growth ...
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bioRxiv - Bioengineering 2020Quote: Extracellular concentrations of glucose and lactic acid from glucose shake flask cultivations were analysed by high-performance liquid chromatography (HPLC) on an Fast acid and Aminex HPX-87H columns (BioRad Laboratories, Hercules, CA) with 2.5 mM H2SO4 as eluant and a flow rate of 0.5 mL min-1 ...
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bioRxiv - Microbiology 2020Quote: ... succinate and ethanol concentrations using a HPLC system (Waters Corporation, Milford, MA) that was equipped with an organic acid column (BioRad Aminex HPX-87) and a refractive detector (Model 2414 ...
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bioRxiv - Neuroscience 2021Quote: Protein lysates from SH-SY5Y cells were quantified (bicinchoninic acid protein assay, Pierce) and then electrophoresed in 4—12% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad) in 1X TGS Tris/Glycine/SDS Buffer (10X TGS Buffer ...
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bioRxiv - Microbiology 2022Quote: ... Marcy l’Etoile, France), sheep blood agar with colistin and nalidixic acid (CNA, bioMérieux) and chromogenic agar (Uriselect 4, Bio-Rad, Marnes-la-Coquette, France). Bacterial growth was determined after 18 h of incubation at 37°C ...