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Citations for Bio-Rad :
1851 - 1900 of 2175 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) assays were conducted on the CFX96 real-time detection system (Bio-Rad, USA) with the SYBR Green PCR Mix (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The RS ratio was calculated from each duplexed reaction by the QX200 Droplet Digital PCR system software (QuantaSoft AP, Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... Droplet digital PCR reactions were performed using the QX200 Droplet Digital PCR system with ddPCR SuperMix for Probes (no dUTP) (Bio-Rad). The RS ratio was calculated from each duplexed reaction by the QX200 Droplet Digital PCR system software (QuantaSoft AP ...
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bioRxiv - Genetics 2022Quote: ... at 37°C for 30 min and then mixed with the ddPCR reaction mix composed of 2X ddPCR SuperMix for probes (no dUTP) (Bio-Rad), forward (for ...
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bioRxiv - Pathology 2023Quote: ... Each individual and multiplexed TaqMan reaction was performed in a final volume of 10 µL containing 5 µL of Sso Advanced Universal Probes Supermix (Bio-Rad), 600 nM of each primer ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl of diluted cDNAs were added to each PCR reaction containing the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, 1725271) and primers at a final concentration of 300 nM each ...
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bioRxiv - Plant Biology 2023Quote: The iTaq-Universal SYBR Green Supermix was used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR) by following the manufacturer’s instructions (Bio-Rad, USA). The first strand cDNA samples were diluted 3 times prior to qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... amplification employed primers targeting Se uvrB or the 16S rRNA gene of Ai and was performed in 20 μL reactions with 1X SsoAdvanced Universal SYBR Green Supermix (Bio-Rad), 300 nM each primer and 4 μL template DNA (primer sequences provided in Table S3) ...
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bioRxiv - Bioengineering 2023Quote: ... 1x DreamTaq buffer and 1 μL sample DNA in a total reaction volume of 10 μL using CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Neuroscience 2023Quote: qPCR reactions were performed using SsoAdvanced Universal SYBR Green Supermix on a CFX96 Touch Real-Time PCR Detection System (Bio-rad). Primer efficiencies were calculated by carrying out a dilution series ...
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bioRxiv - Genetics 2023Quote: ... Reaction mixtures were resolved on a 14% urea-PAGE gel (AmericanBio, Inc.) and analyzed using a PharosFX molecular imager (Bio-Rad). Single-nucleotide extension products were quantified using Image Lab software ...
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bioRxiv - Neuroscience 2023Quote: ... was used to reverse transcribe the total RNA into cDNA in a 20 µl reaction volume using the thermal cycler (T100, Bio-Rad). The QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2023Quote: ... RT-PCR was performed in triplicates and carried out in 20 µl of reaction mixture containing 10 µl of Fast SYBR® Green Master Mix (Biorad), 0.2 µl (10 pmol/µl ...
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bioRxiv - Evolutionary Biology 2023Quote: ... qPCR was performed with gene-specific primers using the Taq Pro Universal SYBR qPCR Master Mix (Vazyme #Q712) reaction system on the CFX96 Real-Time detection system (Bio-Rad). Each experiment was performed with three reaction replicates and UBQ10 was used as the internal control for data analysis ...
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bioRxiv - Genetics 2023Quote: ... and qRT-PCR reactions were run with HOT FIREPol SolisGreen qPCR Mix-reagent (Solis BioDyne, #08-46-00001) using CFX384 machine (Bio-Rad). qRT-PCR data was normalized to the expression of cdc-42 and pmp-3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... we electroporated a small fraction of the assembly reaction into inhouse DH10B cells using 1 mm cuvettes and a micropulser (Bio-Rad) set at 1.8 kV ...
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bioRxiv - Microbiology 2023Quote: ... gDNA templates were normalized to 500 pg/rxn and final 20 ul reactions consisted of 2x ddPCR Supermix for Probes (no dUTP) (Bio-Rad), 20x target primer/probes ...
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bioRxiv - Bioengineering 2023Quote: ... 1 µL of the formed cDNA was used in 10 µL reactions using the SsoAdvanced universal SYBR green supermix (Bio-rad) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was then diluted 1:100 and 1.5 µL was use per 15 µL reaction with iTaq Universal SYBR Green Supermix (Bio-Rad # 1725122) and 250 nM primers (final ...
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bioRxiv - Physiology 2023Quote: ... was used to run the quantitative polymerase chain reaction (qPCR) using the CFX 380 Real-Time system C1000 TM Thermal Cycler (Bio-Rad), with the following parameters ...
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bioRxiv - Genomics 2023Quote: ... 2.0 μL of cDNA was used per PCR reaction with Perfecta SYBR Green Fastmix (Quanta BioSciences, 95072) using the CFX96 Real-Time PCR Detection System (Bio-Rad). All primers were designed to be highly specific using NCBI’s primer blast tool and amplicon products were verified by melt curve analysis ...
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bioRxiv - Cell Biology 2023Quote: 5 to 10 ng of cDNA were used to perform quantitative polymerase chain reaction using 1X SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). All RT-qPCR reactions were performed in a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... The reverse transcription was conducted using 500 ng of RNA in a 20 µl volume reaction mixture of iScript cDNA Synthesis Kit (Bio-Rad). 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in a 20 μL reaction mixture on a Bio-Rad CFX ConnectTM Real-Time PCR System (Bio-Rad, Hercules, CA, USA). To reduce the error ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µl of a 10X diluted cDNA sample was then used in the qPCR reaction with SsoAdvanced Universal SYBR Green Supermix (Bio-rad). Primers were designed using Primer3 software (v4.1.0) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from PMVECs and Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Biochemistry 2023Quote: ... in a 100 μL reaction for 2 hours at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad). The radiolabeled probe was heated to 90°C for 2 minutes and resuspended in 10 mL of ULTRAhyb Oligo hybridization buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... The reactions were performed using the SsoAdvanced Universal SYBR Green Supermix in Hard Shell qPCR plate 96-well thin wall (Bio-Rad). Amplification reactions were performed using an RT-PCR Detection System thermal cycler (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 µl of the diluted solution was used as template in a 15 µl reaction with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). qPCR was performed using CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... Reverse transcription reaction was conducted using 0.5 µg of total RNA and 2 µl of iScript™ Reverse Transcription Supermix (Bio-Rad) in 10 µl reaction volume ...
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bioRxiv - Microbiology 2023Quote: ... mouse cDNA was analyzed in a 10-μl reaction mix consisting of 1x iQ SYBR® Green Supermix (Bio-Rad, USA), 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Chemiluminescent reaction was performed with SuperSignal West Dura substrate (Thermo, 34076).Images were acquired with a ChemiDoc XRS+ system (Bio-rad) and quantified using Image Lab software (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: Immunoassays were performed with 25 μl of conditioned medium or tissue lysate each reaction using a Bio-Plex 200 System (Bio-Rad) with Milliplex Map Mouse Cytokine/Chemokine Panel I (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... A volume of 40 µl of generated droplets per reaction was then transferred to a 96-well plate and foil-sealed (1814040; Bio-Rad) with a thermal plate sealer (PX1 ...
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bioRxiv - Microbiology 2023Quote: ... Each assembled ddPCR reaction mixture was then loaded into the sample well of an eight-channel disposable droplet generator cartridge (Bio-Rad). A volume of 70 μL of droplet generation oil (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: qPCR was run with cDNA previously generated from rescue experiments in a 10 µl reaction using iTaq Universal SYBR Green Master Mix (BioRad 1725121) with readout using the Applied Biosystems ViiA 7 real-time PCR system with standard cycling parameters (50°C for 1 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR (qPCR) was performed in a 10 µL reaction that contained 5 µL SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µL H2O ...
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bioRxiv - Cancer Biology 2024Quote: ... was added to each sample and heated at 95 °C for 1 hour in a thermal cycler (S1000 with 384-well reaction module, Bio-Rad) set to a constant lid temperature of 110 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by incubation at 95°C for 60 minutes in a thermal cycler (S1000 with 384-well reaction module, Bio-Rad). To this ...
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bioRxiv - Bioengineering 2024Quote: The outcome of the reaction was analysed using native PAGE (4-20% Mini- PROTEAN TGX Precast Protein Gels, Bio-Rad, 4561096). 1 µL of the retic lysate reaction was diluted with 2 µL 1×PBS and mixed with 2× sample buffer containing 62.5 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were carried out in duplicates or triplicates with a CFX Connect Real-Time PCR Detection System (Bio-Rad, Munich, Germany). Relative expression was calculated by the ΔΔCt method and normalized against either ribosomal protein lateral stalk subunit P0 (RPLP0 ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from Rat lung tissue and subjected to cDNA preparation by the reverse transcription reaction using an iScript cDNA Synthesis Kit (#17088890, Bio-Rad). qPCR analysis was performed in triplicate using iQ SYBR Green Supermix (#1708882 ...
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bioRxiv - Microbiology 2024Quote: ... RNA extracts were tested by real-time RT-PCR with the Menzel and Winter primers (Menzel & Winter, 2021, EPPO, 2022) using iTaq universal probes one-step reaction mix (Bio-Rad) and 1 µl of RNA extract ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 ng template library DNA[21] in a total reaction volume of 20 µL using T100 Thermal Cycler (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Biochemistry 2024Quote: ... iSAT reactions started after addition of plasmid encoding rrnB under T7 promoter at 4 nM and the reaction was immediately incubated on pre-warmed CFX Connect Real-time System (Bio-Rad) at 37 °C for 90 mins in 96 well plates (Applied Biosystems) ...
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bioRxiv - Physiology 2024Quote: ... Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was performed using a CFX96 Real-Time PCR Detection System (Bio-Rad) with Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... qPCR reactions were performed in a 96-well transparent plate on a Bio-Rad CFX96 Real-Time PCR machine (Bio-Rad) in 10 µl mixtures each containing 5 µl of Takyon™ ROX SYBR® MasterMix dTTP Blue (Eurogentec ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of each diluted RT and NRT sample was added to a 22 µL reaction mixture containing 11 µL of QX200TM ddPCR TM Evagreen Supermix (Bio-Rad) and 1.1 µL of each 2 µM ddPCR primers (Table S6) ...
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bioRxiv - Plant Biology 2024Quote: ... One microgram of total RNA was used for a reverse transcriptase reaction using iScript cDNA Synthesis Kit (Catalog #170-8891; Bio-Rad). RT-qPCR analysis was conducted using iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... was performed with 100 ng of cDNA in a 20-μL reaction mixture containing SsoAdvanced universal SYBR green supermix (#1725274; Bio-Rad) using phagocytosis PrimePCR plates (#10047255 ...