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Citations for Bio-Rad :
1801 - 1850 of 2175 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The reaction mix was incubated for 30 mins at 37°C and purified using Micro-Bio P-30 spin columns (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:4 with nuclease-free water and used as template (4 µL per reaction) for segment-specific qPCR using SsoFast EvaGreen Supermix (Bio-Rad) in 10 µl reactions ...
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bioRxiv - Microbiology 2022Quote: ... added to a 15 μL reaction mixture containing 500 nM primers (sequences in Table 1) and iTaq Universal SYBR Green Supermix (Biorad, # 1725124) using a thermocycler (Mastercycler ep realplex2 ...
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bioRxiv - Physiology 2022Quote: ... The PCR cycle number for each library amplification was optimized by running 10% of the library DNA in a real-time PCR reaction using Brilliant III Ultra-fast SYBR Green QPCR Master Mix (AH Diagnostic) and a C1000 Thermal cycler (Bio-Rad). DNA libraries were sequenced on a HiSeq2000 by 50□bp single□end sequencing at the National High Throughput Sequencing Centre (University of Copenhagen ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for fluorescence and the reaction mixture was amplified (40 cycles) using a CFX384 Touch Real-Time PCR Detection System (Bio-Rad). Gene expression was analyzed using Bio-Rad CFX Manager v3.1.
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bioRxiv - Immunology 2020Quote: ... in a duplex-digital droplet PCR reaction using the Raindrop System (Raindance Technologies, Billerica, MA and Bio-Rad, Hercules, CA, USA). The Raindrop Source instrument was used to generate uniform aqueous droplets (5 picoliter ...
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bioRxiv - Microbiology 2021Quote: ... using a TaqMan-based assay for lipL32 [180] in 25 μl reactions performed with SsoAdvanced Universal Probes Super Mix (Bio-Rad). Copy numbers for lipL32 were determined using an internal standard curve for the lipL32 amplicon cloned into pCR2.1 TOPO (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Droplets were then generated using the QX100 Bio-Rad droplet generator with 20 μl reaction mixture and 70 μl droplet generation oil (#1863005, Bio-Rad). The ESR1 ligand binding domain fragment was amplified in each sample and signals from WT and mutant probes of each droplet were read using the Bio-Rad QX100 droplet reader ...
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bioRxiv - Immunology 2020Quote: ... cDNA was quantified using SYBR-green for quantitative reverse transcription polymerase chain reaction on a CFX Connect Real-Time System (Bio-Rad) with the following amplification scheme ...
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bioRxiv - Molecular Biology 2021Quote: ... and 200 ng of total RNA was subjected to reverse transcription reactions using iScript™ Reverse Transcription Supermix (Bio-Rad, 1708841). SYBR Green was used for amplicon detection and gene expression was normalized to the expression of housekeeping genes Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Microbiology 2020Quote: ... 250 ng of RNA was used as a template in each reaction with iTaq Universal One-Step RT-qPCR Kit (Bio-Rad) or Luna Universal One-Step RT-qPCR Kit (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplification was carried out on a C1000 TouchTM Thermal Cycler with 96-Deep Well Reaction Module (Bio-Rad, CA, USA) using a thermal profile of beginning at reverse transcription ...
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bioRxiv - Microbiology 2020Quote: ... Wild-type CrPV and CrPV-DcDV RNA levels were determined using 4.5 µL diluted cDNA for qPCR reactions with the SsoAdvanced Universal SYBR Green Supermix (BioRad, Hercules, California) in 10 µL reactions ...
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bioRxiv - Cell Biology 2021Quote: ... The reaction was carried out according to the manufacturer’s instructions using CFX Connect Real-Time PCR Detection System (Bio-rad, 1855200). Probes used for TaqMan® Gene Expression Assays (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: Probe-based qPCR was used to amplify cDNA in 20μl reactions performed in triplicate for each sample condition in a PCR plate (Bio-Rad, UK), as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... absolute quantification of the HIV Long Terminal Repeat (LTR) was performed in a duplex-digital droplet PCR reaction using the Raindrop System (Raindrop Technologies / Bio-Rad). The Raindrop Source instrument was used to generate uniform aqueous droplets (5 picoliter ...
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bioRxiv - Microbiology 2021Quote: ... cDNAs (+ and – RT) were assayed in quadruplicate in 25 μl reactions performed with SsoAdvanced Universal SYBR or Probe (lipL32) Super Mixes (Bio-Rad). Oligonucleotide primers used for qRT-PCR are provided in S6 Table ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was carried out in 96-well optical reaction plates using the iQ™ SYBR® Green Supermix (Bio-Rad). The reference gene Actin and gene-specific primers for the RT-qPCR are listed in Supplementary Table S8.
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bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s instructions and qPCR reactions were carried out on CFX96 Thermal Cycler Real Time System (Bio-Rad Laboratories). The reactions were normalized to housekeeping genes (mRlp13a and mS18 ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was performed using the iTaq Univer SYBR Green 1-Step Kit (Bio-Rad) on a StepOnePlus Real-time PCR system (Applied BioSystems ...
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bioRxiv - Developmental Biology 2021Quote: ... or 400 ng of total RNA was reverse transcribed to cDNA in a 20 µL reverse transcription reaction using 5 µL of iScript reverse transcription supermix (1708841, Bio-Rad Laboratories - Life Sciences ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10nM of templates with each promoter type were added to TXTL reactions and incubated at 30 °C for 8 hours (T100 Thermal Cycler, Bio-Rad). Post-incubation ...
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bioRxiv - Zoology 2021Quote: The reactions of qPCR were conducted in the Bio-Rad CFX96 Real-time PCR Detection System (Bio-Rad, Hercules, California, USA) using 20 μL solutions containing 1.6 μL primers ...
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bioRxiv - Cell Biology 2021Quote: ... Real-Time polymerase chain reaction (RT-PCR) was performed on the CFX 384 Touch™ Real-Time detection system (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... This was randomly partitioned into at least 10,000 discrete oil droplets per reaction using microfluidics within the QX200™ Droplet Generator (Bio-Rad, 1864002 ...
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bioRxiv - Bioengineering 2021Quote: ... 2.5 ng double-stranded DNA was added to a reaction mix containing target-specific primers/probe mixes (900 nM primer/250 nM probe per FAM and HEX fluorophore; Bio-Rad, 10042958 Unique Assay ID ...
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bioRxiv - Cancer Biology 2021Quote: ... All real-time PCR reactions were performed using the CFX Connect Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) and the amplifications were carried out using the ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Developmental Biology 2021Quote: ... Barcoded samples were then pooled and aliquoted into individual ChIP reactions with Protein A/G magnetic beads (BioRad; 161- 4013/23) coupled with the desired antibodies (5 ug each of H3K27me3 {Millipore 07-449} ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription and real-time PCR (qPCR) reactions were carried out using iScript cDNA synthesis kit and iQ SYBR Green Supermix (Bio-Rad). qPCR was performed on a Bio-Rad CFX384 Touch™ Real-Time PCR Detection System using the following human primers (Forward and Reverse ...
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bioRxiv - Microbiology 2021Quote: ... and 2 µL of each diluted RT and NRT-PCR sample was added to a 22 µL reaction mixture containing 11 µL of QX200TM ddPCR TM Evagreen Supermix (Bio-Rad) and 1.1 µL of each 2 µM ddPCR primers (Table S3) ...
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bioRxiv - Genomics 2021Quote: ... According to kit instruction 10 μl reaction system was prepared and q-PCR was operated using CFX96 Real-Time System (BIO-RAD). For data analysis ...
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bioRxiv - Microbiology 2022Quote: ... The digital droplet PCR (ddPCR) reaction mixture consisted of 12.5 µL of a 2X ddPCR Supermix for Probes no dUTP (Bio-Rad: 1863024), 1.25 µL of each 20X primer-probe mix ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR amplifications were carried out at least in duplicate in a 25 µL reaction volume on a CFX96 instrument using the CFX manager software (Bio-Rad). Each PCR reaction mixture was composed of 12.5 µl PCR mix (Bio-Rad Sso advanced mix SYBR Green) ...
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bioRxiv - Bioengineering 2022Quote: ... approximately 5 ng of RNA converted to cDNA was used per 10μL well reaction in the Wound Healing PCR Array (Bio-Rad, 10034601). Real-time PCR reaction was prepared using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: ... and the Phusion Green Hot Start II High Fidelity PCR Master Mix (Thermo-Fisher-Scientific) according to the manufacturer’s instructions in a 25 μl reaction on a C1000 Thermo Cycler (Biorad, Hercules, USA). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... Amplification was performed through polymerase chain reaction (PCR) in a Bio-Rad C1000 TouchTM Thermal Cycler (BIO-RAD, Hercules, CA, USA). The V4 region of the 16S rDNA gene was amplified using dual-index (forward and reverse ...
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bioRxiv - Pathology 2022Quote: ... Whole bee specimens were extracted in trizol and then RNA was used to produce cDNA using single reaction reverse transcriptase according to manufacturer specifications (BioRad, IScript). Total viral cDNA was quantified using real time qPCR and a 10 fold dilution series of prepared standards exactly as described in Posada-Florez et al.12.
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bioRxiv - Molecular Biology 2022Quote: ... The 10 µL cDNA reaction was diluted to 200 µL and 3 µL was used with iTaq Universal SYBR Green Supermix (Bio-Rad) and 670 nM of primer pairs (Table S4 ...
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bioRxiv - Plant Biology 2022Quote: ... The dicing reactions were performed at 37 °C for 30 min and stopped by adding 1 volume TBE-Urea sample buffer (Bio-Rad), heating at 95 °C for 10 min and quickly chilling on ice for 2 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The RT reactions were amplified with primers for target RNA and ACTIN (Supplemental Table S2) using iTaq Universal SYBR Green (Biorad, 1725120), and quantified on (Biorad CRX Connect Real-Time PCR Detection System ...
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bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA was reverse transcribed into cDNA in a 10 μl reaction using the iScript cDNA synthesis kit (Bio-Rad). cDNA was then diluted 1:10 and 4 μl was used for quantitative RT-PCR using the LuminoCt SYBR Green master mix on a Roche LightCycler 480 machine ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad) with Micro-Amp Optical 384-well plates ...
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bioRxiv - Cell Biology 2024Quote: ... We produced droplets pipetting 20 µl of the PCR reaction mix into single well of a universal DG8TM cartridge for droplets generation (Bio-Rad). 70 µl of droplet generation oil were also added in each well next to the ones containing the samples ...
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bioRxiv - Genomics 2024Quote: ... 10 ng of cDNA was used per PCR reaction with Perfecta SYBR Green Fastmix (Quanta BioSciences, 95072) using the CFX96 Real-Time PCR Detection System (Bio-Rad). All amplicon products were verified by melting curve analysis ...
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bioRxiv - Immunology 2024Quote: ... cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad, Hercules, CA) and 5 μM each of gene-specific forward and reverse primers ...
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bioRxiv - Immunology 2024Quote: ... 20 µl of each reaction mix and 70 µl of oil were converted to droplets with the QX200 droplet generator (Bio-Rad). Droplet-partitioned samples were then transferred to a 96-well plate ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of synthesized cDNA was firstly diluted in a 1:5 ratio and used in a 20 µL reaction volume using the CFX96 Touch Real-Time PCR Detection System (BioRad, USA) with the following thermal cycling conditions ...
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bioRxiv - Immunology 2024Quote: ... quantitative real-time polymerase chain reaction (qRT-PCR) using gene-specific primers and universal SYBR Green Supermix (#1725274, Bio-Rad Laboratories) was performed ...
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bioRxiv - Biochemistry 2024Quote: ... and 5 to 20 mM imidazole (wash-buffer) until the eluate was protein-free as judged by the lack of reaction with Bradford dye reagent (Bio-Rad). The calpain-3-titin/I81-I83 complex was eluted with 300 mM imidazole in wash-buffer ...
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bioRxiv - Biophysics 2024Quote: ... The DNA strands at 1 mM concentration were incubated with 10 mM of BG-GLA-NHS and reactions were cleaned up using a Bio-Spin P-30 column (Bio-rad).