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Citations for Bio-Rad :
2051 - 2100 of 3179 citations for Interstitial collagenase MMP 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... diluted 1:15000 in blocking buffer) followed by a goat anti-rabbit-HRP conjugate (170-6515, Bio-Rad, Hercules ...
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bioRxiv - Developmental Biology 2022Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) (Bio-Rad, Hercules, CA) or anti-mouse IgG-HRP conjugate (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal total protein concentration were heated at 90°C in 1 × loading buffer (Bio-Rad 1610747) for ten minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Genomics 2022Quote: Western Blot samples of unbound fractions and IP were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...
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bioRxiv - Neuroscience 2022Quote: ... horseradish peroxidase (HRP) conjugated secondary antibodies (1: 5000) and signal detected using ClarityTM Western ECL Substrate solution (BioRad). Blots were visualised on a ChermiDOC XRS+ system (BioRad ...
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bioRxiv - Bioengineering 2019Quote: ... Blots were subsequently incubated with 0.33 µg ml-1 goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Immunoreactive bands were visualized using horseradish peroxidase-conjugated goat anti-rabbit IgG (Bio-Rad #170-6515; 1:3000), followed by ECL detection (GE Life Sciences) ...
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bioRxiv - Plant Biology 2020Quote: ... 1 µg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix (Biorad ...
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bioRxiv - Biochemistry 2019Quote: Complementary DNA was synthesized from 1 μg of purified RNA using the iScript cDNA Synthesis Kit (BioRad, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Both PK-treated and untreated samples were then mixed 1:2 with 4X Laemmli sample buffer (Bio-Rad) containing DTT ...
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bioRxiv - Microbiology 2019Quote: ... and 1 mM phenylmethylsulfonyl fluoride (PMSF) and protein concentration was determined using the DC protein assay (Bio-Rad). Whole cell lysates were resolved by SDS-PAGE in 4-20% gradient Mini-PROTEAN TGX gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at RT and detected using Clarity or Clarity Max Western ECL Blotting Substrate (Bio-Rad) and imaged (Amersham AI600) ...
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bioRxiv - Cell Biology 2019Quote: ... Immune complexes were detected with horseradish peroxidase-conjugated anti-rabbit secondary antibodies (1:5,000 dilution; Bio-Rad 1706515).
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bioRxiv - Developmental Biology 2019Quote: ... cells were incubated for 1 h on ice and finally measured using the S3e™ Cell Sorter (BioRad) apparatus ...
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bioRxiv - Evolutionary Biology 2020Quote: ... NCBI accession DQ278441.1) (Table 1) were used for quantitative PCR using the iQ SYBR Green Supermix (BioRad, Canada) and the following cycling conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized from 1 mg of RNA using the iScript cDNA synthesis kit (Bio-Rad), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Blots were subsequently incubated with 1:2000 dilution of goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at RT ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... The membrane was incubated with a primary mouse anti-pig IL-18 antibody (diluted 1:500, Bio-Rad) or anti-β-actin antibody (diluted 1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 h at rtp with 5% (w/v) non-fat milk block (Bio-Rad) in 1x TBS (in mM ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 30 min at room temperature with α-CD3 (1 µg/mL; clone UCHT1; Bio-Rad). CD8+ T cells were negatively selected from blood and TDL using a CD8+ T Cell Enrichment Kit (stemCell Technologies) ...
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bioRxiv - Immunology 2019Quote: ... was detected with goat anti-rabbit AF 647 and sheep anti-TGN46 primary antibody (Biorad, AHP500, 1:100) was detected with donkey anti-goat AF 647 (1:500).
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bioRxiv - Immunology 2019Quote: ... purified PR3 mutant proteins were loaded (1 µg/lane) onto 12% Tris-HCl gels from BioRad (Hercules, CA). The SDS gel electrophoresis was performed at 180 volts for 35 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... then 3 washes in 1% milk were performed prior to imaging the blot with ECL (BioRad Clarity #1705060). Membrane was stripped using the GM Biosciences One Minute Advance Stripping buffer #GM6031 ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated with Goat Anti-Mouse IgG (Heavy + Light)-HRP conjugate (170-6516, 1:1,000; Bio-Rad) overnight and following PBS-T washing detected using the LAS-500 Imaging System (General Electric).
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bioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription of 1 μg of RNA was carried out with iScript cDNA Synthesis Kit (1708891, Bio-Rad) using an extended transcription step of three hours at 42°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4970) overnight at 4 °C and the HRP-conjugated secondary antibodies (BIO-RAD, Cat. No. 1706515, 1:1000) for 1 h at room temperature ...
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bioRxiv - Physiology 2020Quote: ... and reverse transcribed into cDNA using 1 μg of RNA and iScript Reverse Transcriptase (Bio-Rad, Hercules, CA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... incubated with primary antibody (monoclonal rat anti-F4/80, 1:200 dilution, MCA497; BIO-RAD; Hercules, CA, USA) for one hour ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked 1 hour in skim milk 5% TBS-Tween buffer (TBST, Bio-Rad, 0.05% Tween 20), and incubated overnight with the primary antibodies diluted in blocking buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10% (19:1) PAGE was prerun for 30 minutes at 100V in a Mini PROTEAN tank (Bio-Rad) electrophoresis chamber using 1X TBE (89 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies were used: mouse anti-talin1 (clone 97H6, Bio-Rad: MCA4770, Lot#:1803, 1 μg/ml), rabbit anti-zyxin (B71 ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM DTT) before being loaded on a HiTrapQ anion exchange column using the NGC FPLC (Bio-rad). PfQC was eluted using a salt gradient ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 ug of total RNA was converted into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad). RT-qPCR was performed using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Hydrogels of varying stiffness were prepared using varying ratios of 40% acrylamide to 1% bis-acrylamide (Bio-Rad) in a mixed solution with water ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-tubulin YL1/2 from rat (MCA77G, Bio-Rad AbD Serotec, at 1:2000 dilution for IF), anti-rabbit Alexa Fluor 488 (A11008 ...
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bioRxiv - Neuroscience 2020Quote: ... Three replicas of 1.5μl of a 1:3 dilution of cDNA were amplified using SsoFast EvaGreen Supermix (BioRad) for FACS-sorted microglia and Power SYBR Green (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... 25 µg (1 µg/µL concentration) of plasmid DNA was added to electroporation 4 mm cuvettes (Bio-Rad) first and then cells added to the cuvettes ...
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bioRxiv - Neuroscience 2022Quote: Goat anti-mouse IgG (H+L) horseradish peroxidase-conjugated secondary antibody (Bio-Rad, 1:5000; Cat. 170-6516,) and Goat anti-rabbit Igg (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to cDNA (1 ug RNA per reaction) using the iScript cDNA Synthesis Kit (Bio-Rad) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 μg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix and amplification was run on a CFX96 thermal cycler (BioRad) ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized using a 1-μg aliquot of RNA and the iScript cDNA Synthesis system (Bio-Rad). qPCR was performed using human-specific primers (Table S1 ...
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bioRxiv - Immunology 2022Quote: ... and complementary DNA was reverse transcribed from 1 μg total RNA using iScript cDNA Synthesis Kit (Bio-Rad). mRNA expression was measured using iQ SYBR Green on a CFX96 Real-Time System (Bio-Rad) ...
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bioRxiv - Genomics 2022Quote: ... OrgangeG gel loading dye was mixed with PCR product and ran in a 1% agarose gel (Bio-Rad Certified Molecular Biology Agarose 1613102 ...
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bioRxiv - Genomics 2022Quote: ... using 0.5 × Tris-Borate-EDTA buffer and a 1% gel with low melting point agarose (Bio-Rad #1620137). Gels were stained with 5 μg/ml ethidium bromide in water after electrophoresis for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... This was followed by incubation with HRP conjugated secondary antibody (1:10000) and developed with ECL solution (BioRad).
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bioRxiv - Physiology 2022Quote: ... 1 μg of total RNA was reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad, CA, USA). In Quantitative real-time PCR (qPCR) ...
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bioRxiv - Immunology 2022Quote: ... supernatants were diluted at 1:4 and measured by Bio-Plex Pro mouse IFN-γ immunoassay (Bio-Rad) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... and stat1a- or stot1b-specific primers (Table 1) in a CFX96 touch RT-PCR detection system (Bio-Rad) using Hardshell® Low Profile Thin-wall 96-well skirted PCR plates (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... the membrane was incubated with 1:10,000 dilution of anti-mousehorseradish peroxidase (anti-mouse-HRP) antibody (Bio-Rad) at room temperature for 1 h and washed again three times in TBST ...