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Citations for Bio-Rad :
2251 - 2300 of 3179 citations for Interstitial collagenase MMP 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... membranes were rinsed threetimes in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Plant Biology 2022Quote: ... USA (17) and a 1:3000 dilution of goat anti-rabbit IgG horseradish peroxidase (HRP) conjugate secondary antibody (Biorad). Chemiluminescence was detected using WesternBright ECL (Advansta ...
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bioRxiv - Microbiology 2022Quote: ... A 15% SDS-Tricine gel was made by mixing: 2.5 mL 30% acrylamide/Bis solution 37.5:1 (BioRad #1610158), 1.25 mL gel buffer (1.5 M Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was subsequently washed and incubated in HRP-conjugated secondary antibody against the appropriate host (1:10,000, BioRad) for 1 hour RT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... together with 2.5 µl of single (1 µl assay + 1.5 µl NF water) or multiplexed (0.5 µl of each assay) PrimePCR Probe Assays (Bio-Rad) as follows with single or multiplex reactions grouped in parentheses and formatted as Gene/UniqueAssayID ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was synthesized from 1 μ of total RNA using iScript gDNA clear cDNA synthesis kit (Bio-Rad, 1725035) with random primers ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed on 1 μg of RNA using the iScript™ cDNA Synthesis Kit (Bio-Rad #1708891). Then ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli and plasmid DNA were electroporated in 1 mm cuvettes at 1.8 kV using the MicroPulser electroporator (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... We synthesized the first strand cDNA with 1 µg of total RNA using iScriptTM Reverse Transcription Supermix (BIO-RAD) followed by quantitative PCR with CFX96 Real-Time System (BIO-RAD ...
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bioRxiv - Cancer Biology 2024Quote: Cells were first embedded in gel plugs with approximately 1 million cells per gel plug (BioRad no. 170-3592). The high molecular weight genomic DNA was extracted and purified using the Bionano Genomics SP kit.
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bioRxiv - Cell Biology 2024Quote: Polyacrylamide gel electrophoresis was carried out using 10 % gels (acrylamide/bis-acrylamide ratio 37.5:1) or any kDa precast gels (Biorad) and a MiniProtean (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... Cell lysates and purified supernatants were separated on an SDS-PAGE and analyzed through immunoblotting against vinculin as a loading control (1:2,000, MGA465GA, BioRad) or CHIKV E1 glycoprotein (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of the RNA was reverse transcribed into cDNA with the iScript Advanced cDNA Synthesis kit (Bio-rad). 20 μL of the end products were diluted by adding 180 μL nuclease-free water and stored at -20 °C until further analysis.
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated goat anti-rabbit IgG (1:5000; 1706515, Bio-Rad) at RT for 1 h followed by chemiluminescent detection with ECL Substrate (1705060 ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription (RT) was performed on 1 µg RNA with the iScript™ Reverse Transcription Supermix (Bio-Rad 1708841) for 5 min at 25°C ...
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bioRxiv - Genomics 2024Quote: ... F43E2.6 siRNA and miR-1 Ct values were assayed using a CF96 Real-Time PCR Detection System (Bio-Rad). Ct values were averaged across three technical replicates for each of 3 biological replicates.
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bioRxiv - Plant Biology 2024Quote: ... cDNA was generated from 1 μg of RNA using the iScript Reverse Transcription Supermix for RT-qPCR kit (BioRad). PCR with Chr07:9757891 For and Chr07 9758683 Rev primers was performed using APEX master mix ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using candidate primers (Integrated DNA Technologies; Table 1) and an iScript cDNA Synthesis Kit (Bio-Rad). Relative mRNA expression was analyzed by quantitative RT-PCR using the QuantStudio PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... and goat anti-rabbit (Cat. # 170-6515) antibodies were used at 1:2000 for immunoblotting and purchased from Biorad.
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bioRxiv - Genomics 2024Quote: ... samples were embedded in a thin polyacrylamide film by inverting them onto a GelSlick-coated microscope slide with a droplet of 4% acrylamide solution (4% v/v 20:1 acrylamide:bis-acrylamide [BioRad, 1610144] with 0.15% v/v TEMED [Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Caspase activity was measured as described in the FAM FLICA caspase 1 and 3 kit (Bio-Rad, Hercules, USA). Lysosomal and mitochondrial content as well as production of ROS were analysed by staining with 50 nM LysoTracker™ Deep Red ...
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bioRxiv - Immunology 2024Quote: ... qPCR was then performed with a 1:10 dilution of cDNA using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a QuantStudio 6 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: 0.2 – 1 mL (sufficient to cover the sample) of 5% Chelex 100 Resin (Bio-Rad Laboratories, Hercules, CA, USA) with 0.2% Tween20 and 0.1 mg/mL proteinase K (Merck ...
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bioRxiv - Immunology 2024Quote: ... Amplicons were resolved in a 1% agarose gel and visualized using the ChemiDoc MP Imaging System (Biorad, Hercules, CA). When specified ...
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bioRxiv - Neuroscience 2023Quote: ... each primary was detected via HRP conjugated Goat α-Rabbit secondary antibody (1:4000, Bio-Rad, product 170-6515) after incubation with Clarity ECL substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked for 1hr in PBS with 1mg/ml and 0.1% triton and then stained overnight with anti-alpha Tubulin antibody (1:100, MCA78G, BioRad). Cells were then washed ...
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bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were probed with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1:10,000, 52200336 and 52200341, Kirkegaard & Perry Laboratories) and detected by enhanced chemiluminescence using a Chemidoc (BioRad) gel imaging system ...
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bioRxiv - Genomics 2023Quote: ... An aliquot of the DNA was resolved on 1% agrose gel and visualized on ChemiDoc Touch Imaging System (Biorad) to determine the quality of the tagmented libraries ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used for immunofluorescence at the indicated concentrations: α-Tubulin (Bio-rad, MCA77G, 1:500), β-Tubulin (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed with TBST and incubated with StarBright Blue 700 Goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 hour ...
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bioRxiv - Genetics 2023Quote: ... ∼25 μL sample was run out on a 7.5% SDS-PA at 150V for ∼1 h alongside a protein standard (BioRad Precision Plus Protein Dual Color Standard) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was normalized to 1 mg/mL using 0.1% Azo buffer by the Bradford assay (Bio-Rad). Samples were reduced with 30 mM DTT for 1 h and subsequently alkylated with 30 mM iodoacetamide for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used were the following: HRP-conjugated goat anti-mouse IgG (1:10000, Bio-Rad, 170-6516); HRP-conjugated goat anti-rabbit IgG (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... Axonal staining and myelination in the SLM were assessed with rat anti MBP (BIO-RAD, cat # MCA409S 1:500) and mouse anti-Neurofilament H (NF-H) ...
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bioRxiv - Genetics 2023Quote: ... The membrane was blocked 1 hr with 5% Blotting Grade Blocker Non-Fat Dry Milk (Bio-Rad, Cat# 1706404XTU) and incubated overnight with the primary antibodies [TTC3 Polyclonal Antibody (Abcam ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1×108 concentrated fungal protoplasts (37 μL) (64) were mixed with 2% CleanCut Agarose (63 μL, Bio-Rad, #1703594). The mixture was then loaded into a plug mold (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Thawed lysates were run on 7.5% Acrylamide gels with a 29:1 ratio Acrylamide:Bis using a Protean Tetra system (Bio-Rad) and Precision Plus markers (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using RNAeasy kit from Qiagen (#74104) and 1 ug of RNA was used to synthesize cDNA using iScript cDNA Synthesis Kit (#1708891) from BioRAD. qRT-PCR was performed using TaqMan universal PCR mix (#4324018 ...
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bioRxiv - Microbiology 2023Quote: ... diluted to 20 ng/µL in molecular grade water and 1 µL used for Sybr Green reactions as per manufacturer’s instructions on a BioRad CFX384 (BioRad). Relative expression was calculated by ΔΔCt method to GapDH 66 ...
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bioRxiv - Developmental Biology 2023Quote: ... and cDNA was synthesized from 1 µg of RNA using the iScript™ Reverse Transcription kit (Bio-Rad, USA). qRT-PCR was performed using gene-specific primers for eef1a1l1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative Real rime PCR (RT-qPCR) analysis was performed on genes of interest (Supplementary Table 1) using SYBRGreen (BioRad). Sso Fast EvaGreen Supermix was performed to analyze gene expression on a BioRad CFX96 Real-Time System (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: ... Complementary DNA was then synthesized from 1 μg of total RNA using iScript cDNA Synthesis Kit (1708890, Bio-Rad) and Bio-Rad T100 thermocycler ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was diluted 1 in 10 and 4µl of diluted supernatant was added to 200µl of Bradford reagent (Bio-Rad) at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ug of RNA was diluted into 15 uL reverse transcription reactions using the iScript RT kit (Bio-Rad) and reactions were carried out following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: After washing with TBS-T 0.1% membranes were incubated with goat anti-mouse (1:1000, Bio-Rad, Madrid, Spain), goat anti-rabbit (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... For quantitative real-time PCR 20 ng cDNA and 1 pmol/μl primers were mixed with 2x SYBR (BioRad) in a total volume of 13 μl for each well ...
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bioRxiv - Microbiology 2023Quote: ... in 1×TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo Transfer System (BioRad). Blocking was performed in a 5% solution of dry milk and 0.05% Tween 1×PBS (1×PBST ...
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bioRxiv - Immunology 2023Quote: ... in non-reducing conditions and run at 120 V in 3-Morpholinopropane-1-sulfonic acid (MOPS) buffer (Bio-rad). Bands were visualized with Imperial Protein Stain (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: Diluted mouse serum (1:100 in 0.1% BSA in PBS) was incubated on HEp-2 slides (MBL or BioRad) for 1 hour in the dark at room temperature ...
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bioRxiv - Genomics 2023Quote: ... and 1 µg of RNA was used to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, #1708890). We used RT-qPCR to quantify MYC expression (forward primer ...