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Citations for Bio-Rad :
1851 - 1900 of 3179 citations for Interstitial collagenase MMP 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and Goat pAB to RB IgG HRP (Abcam were both used at 1:10000. Subsequently BioRad Clarity Max Western ECL Substrates were applied and the final results were imaged using a GelDoc.
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bioRxiv - Genomics 2024Quote: ... the membrane was incubated with 1:10,000 dilution of HRP-conjugated anti-rabbit IgG (BioRad, 1706515) for 1 h at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... 1 µg of RNA was used to synthesize cDNA with iScript™ cDNA Synthesis Kit (Biorad), and 100 ng cDNA was used as a template for the reaction ...
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bioRxiv - Biophysics 2024Quote: ... and applied to a 5 mL prepacked Mini ceramic hydroxyapatite (CHT) type 1 column (Bio-Rad). The flowthrough was collected and purified by SEC using a HiLoad 16/600 Superdex 200 (Cytiva ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with tris buffered saline (TBS) with 1% casein blocker (Biorad, Cat. No: 1610782) for one hour ...
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bioRxiv - Biochemistry 2024Quote: ... 1-20 μg of total protein was resolved on a 4-20% polyacrylamide gel (BioRad, 4561096) at 100 V for 60-90 minutes ...
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bioRxiv - Microbiology 2024Quote: ... the film was incubated with 1:2500 goat anti rabbit HRP antibody (BIO-RAD cat. #1706515) for an hour before visualization.
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bioRxiv - Physiology 2024Quote: ... exposed to a primary rat anti-mouse F4/80 monoclonal antibody (1/200) (MCA497G, BioRad, USA). A rabbit anti-rat immunoglobulin (1/200 ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg of RNA was reverse transcribed to cDNA using iScript cDNA Synthesis Kit (BioRad). QPCR was performed with cDNA using the SsoAdvanced Universal SYBR Green Supermix (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... followed in each case by anti-mouse anti-IgG secondary antibody (1:5,000 dilution; Bio-Rad). Immunofluorescent microscopy of T ...
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bioRxiv - Cell Biology 2024Quote: ... ATG16L1 P-S278 antibody was diluted at 1:4000 in EveryBlot blocking buffer (Bio-Rad, 12010020) and incubated overnight ...
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bioRxiv - Microbiology 2024Quote: ... Electroporation was done in a 1 mm cuvette using a Gene Pulser Xcell (BioRad, München, Germany) with a constant time protocol with input parameters 1.5 kV ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed with TBST and incubated with HRP conjugated secondary antibodies (Bio-Rad, 1:10,000) at room temperature for 1h for ECL chemiluminescence detection system (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... the blots were washed and incubated with a 1:3000 dilution of goat anti-rabbit (BioRad) or anti-mouse secondary antibodies (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was prepared from 1 μg total RNA using iScriptTM Reverse Transcription Supermix (Bio-Rad, cat#1708841), then diluted 10-fold and used directly as the template for quantitative real-time RT-PCR (qRT-PCR) ...
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bioRxiv - Microbiology 2020Quote: ... Detection via secondary antibody was done with goat α-rabbit mAb (1:5000) conjugated to HRP (Biorad). Immunoblots were developed using the SuperSignal West Femto (Thermo ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM pH 8.0 by two cycles of centrifugal gel filtration (Biospin mini columns, 6,000 MWCO, Biorad). The nsp’s were buffer-exchanged into 250-500 mM NH4OAc ...
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bioRxiv - Cell Biology 2020Quote: ... StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500, Bio-Rad, Cat# 12005870, RRID: AB_2884949) and hFAB Rhodamine Anti-Tubulin Antibody (1:2500 ...
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bioRxiv - Microbiology 2020Quote: ... and 1 µL used for Sybr Green reactions as per manufacturer’s instructions on a BioRad CFX384 (BioRad). % recovery was calculated as 2 raised to the adjusted input Ct minus IP Ct multiplied by 100 ...
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bioRxiv - Cell Biology 2020Quote: ... The secondary antibodies used for western blot and IF include: goat anti-rabbit HRP (1:10,000, BioRad), goat anti-mouse HRP (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... as well as Plet1 (1:200; Nordic MUbio MUB1512P conjugated to Bio-Rad ReadiLink 633/655 1351005). Single-cell suspensions for ESCs and EPSCs undergoing ES-to-TSC reprogramming as well as control cell lines (ESC/EPSC day 0 samples ...
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bioRxiv - Genetics 2021Quote: ... or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706516). Membranes were incubated in ClarityTM western ECL substrate (Bio-Rad laboratories ...
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bioRxiv - Developmental Biology 2021Quote: ... A total of 1 µg of RNA was reverse transcribed using iScript reverse transcription supermix (Bio-Rad). Real-time PCR was performed as triplicates on 2 ng of cDNA using SYBR Green master mix (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized from 1 μl/RNA sample using an iScript kit (Bio-Rad, catalog #1708891), and PlxnA4 expression levels were measured by qRT-PCR reaction using KAPA SYBR FAST ABI prism qPCR kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of RNA was used for reverse transcription using the iScript cDNA synthesis kit (Bio-rad). The gene expression analysis was carried out using the SsoAdvancedTM Universal SYBR Green Supermix (Bio-rad ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE and observed by immunoblot using rabbit anti-Ube2N (Bio-Rad, AHP974, 1:1000).
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bioRxiv - Molecular Biology 2019Quote: ... and run in 1×TAE buffer for 50h in CHEF-DRIII System with chiller unit (Bio-Rad) with following parameters ...
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bioRxiv - Plant Biology 2019Quote: ... 5 µg of plasmid DNA were bound to 3 mg gold micro-carriers (1 µm, Bio-Rad) by adding 50 µL of 2.5 M CaCl2 and 20 µL of 0.1 M spermidine ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were assessed using 6% polyacrylamide gel (made with 29:1 Acrylamide/Bis Solution, Bio-Rad) electrophoresis ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were then pulsed with 1.8 kV in a 1-mm-gap cuvette (Bio-Rad, USA).
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bioRxiv - Genetics 2019Quote: ... incubated in goat anti-rabbit IgG horseradish peroxidase secondary antibody (1:3000; Bio-Rad, Hercules, CA, USA) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... and 1 μg of RNA was transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad). Primer pairs MFS17/MFS18 ...
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bioRxiv - Microbiology 2021Quote: ... dried for 1 hour at 70°C on a BioRad Model 583 gel dryer (BioRad, Hercules, CA), and protein visualized with Coomassie staining ...
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bioRxiv - Cancer Biology 2020Quote: ... for smaller pellets 50 µl 1% SDS (Sodium Dodecyl Sulfate, Bio-Rad Laboratories Inc., Hercules, CA, USA) was used.
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of RNA was reverse transcribed into cDNA using the Reverse Transcription Kit (Bio-Rad, USA). qRT-PCR was carried out using SYBR Green (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 min and then imaged for 300 s using the ChemiDoc imaging system (Bio-Rad # 17001401). Dialyzed laminin was adsorbed directly to wells to generate a standard reference curve for quantitation ...
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bioRxiv - Bioengineering 2020Quote: ... 0.4% β-mercaptoethanol) per lane or 1 μL of strep-tagged unstained protein standards (Bio-Rad # 1610363) and run at 100 V for 2 h in Tris-HCl-Glycine-SDS running buffer ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 min and then imaged for 120 s using the ChemiDoc imaging system (Bio-Rad # 17001401). Blots were analyzed using ImageLab software (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... followed by a one hour incubation in the appropriate secondary antibodies coupled to peroxidase (1:1000, Biorad). Proteins were detected by enhanced chemiluminescence detection (ECL ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μl cDNA (diluted 1:5) using a CFX384 real-time system qRT-PCR machine (BioRad). Primers were designed using Benchling and purchased from Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes for 1 hour using the Trans-Blot turbo (BioRad) system using standard conditions ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected with a horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, #170-6515, Biorad or #12-349 ...
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bioRxiv - Microbiology 2020Quote: ... Data were quantified using a Personal Molecular Imager with Quantity One 1-D analysis software (Bio-Rad) and analyzed with GraphPad Prism 4 software.
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 mM EDTA) using the Mini-PROTEAN Tetra Vertical Electrophoresis cell (gel size 10×8 cm, BioRad). Each run was preceded by a 30 min pre-run at 300 V to warm the gel ...
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bioRxiv - Microbiology 2021Quote: ... followed by secondary HRP- conjugated goat anti-mouse IgG(H+L) (1:10,000 dilution; Bio-Rad, USA), and then developed using the enhanced chemiluminescence procedure (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of RNA was retro-transcribed using the iScript cDNA Synthesis Kit and polydT primers (Biorad). The cDNA was amplified using the primers listed in S2 Table and the SsoAdvanced universal SYBR Green supermix (Biorad) ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse (#176516; 1:10,000 for Western blot) was from Bio-Rad. Alexa Fluor-conjugated goat antibodies against mouse IgG (1:500 for IF ...
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Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia colibioRxiv - Microbiology 2021Quote: A 1 mL Ni-NTA resin in a glass gravity-flow column (Econo-Column®, BioRad, USA) was set up inside a Coy anaerobic glovebox with an atmosphere of 100% N2 ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour at room temperature and developed with Clarity™ Western ECL substrate (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2021Quote: ... Quantitative Real-Time PCR (qRT-PCR) was carried out using SYBR green 1 chemistry (BioRad, Hercules, CA). Samples for qRT-PCR experiments were assayed in triplicate using the BioRad CFX96 Q-PCR machine ...