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Citations for Bio-Rad :
151 - 200 of 1482 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... at either 95°C for 5 minutes or at 70°C for 10 minutes and resolved in a 4-15% Precast Gels (Bio-Rad, 12-well ...
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bioRxiv - Immunology 2023Quote: ... extension at 72°C for 30sec and final extension at 72°C for 5minutes) were conducted in Thermocycler (Bio-Rad, USA). Positive control samples were taken from Molecular Parasitology Laboratory ...
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bioRxiv - Neuroscience 2024Quote: ... and then melting curve analyses were performed for each reaction to confirm single amplified products between 65-95°C in 0.2°C increments on the Bio-Rad CFX96 system (Bio-Rad, USA). Relative quantification of mRNA of target genes across several time points was determined by taking the ratio of CT values divided by the CT values of glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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Self-assembled DNA-collagen bioactive scaffolds promote cellular uptake and neuronal differentiationbioRxiv - Bioengineering 2024Quote: ... The macrostructure was assembled by heating the primers at 95 °C for 30 min and then cooling them to 5 °C with a step decrease of 5 °C every 15 min using a PCR instrument (BioRad, USA). The resulting macrostructure was then stored at 4 °C until required ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated for 5 minutes at 95 °C followed by 60 minutes at 60 °C using Thermocycler (Bio-rad, U.S). The samples were stored in −20 °C until use ...
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bioRxiv - Bioengineering 2024Quote: ... a thermal gradient across 7 different temperatures ranging from 59 °C to 69 °C was used for each primer set in a T100 Thermal Cycler (Bio-Rad). 5 ng of DNA was added to each well in 20 μL reactions and a no template control (NTC ...
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bioRxiv - Microbiology 2024Quote: ... The hypervariable region V3–V4 of the bacterial 16S rRNA gene was amplified using the primers 338F (5’-ACTCCTACGGGAGGCAGCAG-3’)/ 806R (5’-GGACTACHVGGGTWTCTAAT-3’) by using a T100 Thermal Cycler PCR thermocycler (BIO-RAD, USA). The PCR reaction mixture (20 µL ...
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bioRxiv - Systems Biology 2020Quote: ... 50 mM DTT) and 3 mL oil (Biorad #1864006). After encapsulation ...
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bioRxiv - Cell Biology 2021Quote: ... 3-8% Criterion XT tris- acetate gel (#3450130, Biorad) or 4-15% Criterion TGX gel (#5671083 ...
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bioRxiv - Neuroscience 2022Quote: ... and analyzed with Opticon Monitor 3 and GeneX (BioRad) software ...
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bioRxiv - Immunology 2024Quote: ... Cells were then fixed in 3% paraformaldehyde (Bio-rad) in PBS for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... from a 3% low range agarose gel (BioRad, 1613107) to remove adapter dimers.
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bioRxiv - Biophysics 2024Quote: ... 0.2% (w/v) Bio-Lyte 3/10 Ampholyte (BioRad), 1% bromophenol blue) ...
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bioRxiv - Immunology 2020Quote: ... 40 cycles of 15 s 95°C and 1 min 60°C) was carried out using a CFX 96 (BioRad, Hercules, USA) qPCR machine ...
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bioRxiv - Neuroscience 2021Quote: ... was incubated at 65°C for 10 min and at 85°C for 1 min into a T100™ thermocycler (Bio-Rad). The heated lysate was subsequently used in cDNA synthesis with SuperScript™ III Reverse Transcriptase kit in a total volume of 20 μl following manufacturer protocol (Invitrogen™) ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected following centrifugation of the homogenate at 18,000 gravity for 20 minutes at 4°C and then stored at -80°C until analysis by Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad Laboratories) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The mixture was then incubated at 50°C for 30min followed by 85°C for 15sec in a 9600 thermocycler (Bio-rad, Germany).
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bioRxiv - Plant Biology 2023Quote: ... and CCD1 (5’-GGGAAGAGGGTGATGAAGTTGT-3’ and 5’-TGATATCCATTCACCTTGTCCAAA-3’) and 5 µl of SsoAdvanced Universal SYBR Green Supermix (172-5270; BioRad, Hercules, CA, USA). All samples were analyzed in triplicate using the CFX connect Real-Time PCR Detection System (1855201 ...
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bioRxiv - Developmental Biology 2020Quote: ... Immuno-Star Western C Kit (BioRad catalog 170-5070) was used ...
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bioRxiv - Physiology 2022Quote: ... rabbit polyclonal anti-Cytochrome C (AHP2302, BioRad, 1:200), and mouse monoclonal anti-β-actin (A5441-.2ML ...
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bioRxiv - Genetics 2022Quote: ... Precision Plus Protein Western C Blotting Standards (Bio-Rad) were used for molecular weight standards.
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bioRxiv - Molecular Biology 2020Quote: ... incubated at 95 °C in a thermocycler (Bio-Rad) for 3 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and boiled at 95°C in 1x Laemmli (Biorad) for 7 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... then 72°C for 7 min (BioRad T100 Thermocycler). PCR products were separated on a 2% agarose gel in TAE buffer ...
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bioRxiv - Microbiology 2020Quote: ... denatured at 95°C for 2 min and amplified for 24 cycles (95°C 15 seconds, 60°C, 4 min) on T100 PCR instrument (Biorad, Hercules, CA, USA). At the end of this process ...
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bioRxiv - Biochemistry 2020Quote: ... Plates were blocked with 3% nonfat dry milk (BioRad, P1379) in 1xPBS for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... 3 was performed using an iCycler thermocycler (Bio-Rad laboratories) with a TaqMan RT-PCR Master Mix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... on 3 technical replicates using PCR SYBR GreenSuperMix (1725261, BioRad) and primers listed in Table S1 ...
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bioRxiv - Genomics 2023Quote: ... 5′-CCCCCCATCTGATCTGTTTCAC-3′) by qPCR using SsoFast EvaGreen Supermix (BioRad), according to the manufacturer’s protocol.
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bioRxiv - Pathology 2024Quote: ... plus 0.02% v/v pI 3–10 ampholytes (Bio-Rad). IEF was performed using 11 cm strips ...
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bioRxiv - Physiology 2024Quote: Native-PAGE gels (3% acrylamide:bis-solution 19:1 (BioRad, 1610144)/0.08% ammonium persulfate/0.006% tetramethylethylenediamine (TEMED)/1X TBE ...
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bioRxiv - Biophysics 2024Quote: ... Immobilized pH gradient (IPG) (pH 3–10) strip (ReadyStrip, BioRad) was loaded with proteins dissolved in 125 μl of rehydration buffer and passively rehydrated overnight at 25 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A temperature gradient of 25 to 70°C (heating rate 1°C per min) was applied using a MJ minicycler (Bio-Rad, Hercules, CA, USA). All experiments were performed triplicates.
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bioRxiv - Evolutionary Biology 2020Quote: ... touchdowns from 56 °C to 50°C for 1 min and 50 cycles with 95 °C for 30 s and 50 °C for 1 min in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... followed by 40 cycles (95°C for 5 seconds, 60°C for 40 seconds) on a CFX96 Real-Time PCR System (Bio-Rad, Hercules, CA, USA). The housekeeping gene GAPDH (PY17X_1330200 ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with C-kit variant 1 forward primer 5’-CAACAAAGAGCAAATCCATCCC-3’ and reverse primer 5’-CATCACAATAATGCACATCATGCC-3’ with iQ SYBR Green supermix (BioRad, Hercules, CA, Cat No. 1708882). The PCR program was as follows ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C in a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked in 3% non-fat dry milk in TBS-T buffer for 30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... and cooled to 4°C using a thermal cycler (Biorad). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... hold at 4°C in a C1000 Thermal Cycler (Biorad). We then performed RT-qPCR for gene expression quantification of the following genes ...
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bioRxiv - Microbiology 2021Quote: ... at 8°C in a MyCyclerTM thermal cycler (Bio-Rad). At the indicated intervals ...
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bioRxiv - Molecular Biology 2021Quote: ... Precision Plus Protein Western C Standards (Bio-Rad, 161-0376) were used to determine molecular weights ...
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bioRxiv - Neuroscience 2024Quote: ... held at 4 °C (C1000 Touch Thermal Cycler; Bio-Rad). Preamplified cDNA was immediately diluted 1:5 with 20 uL DNA Suspension Buffer and stored at –20 °C until use.
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bioRxiv - Microbiology 2023Quote: ... and held at 4 °C in a thermocycler (Bio-Rad). The newly synthesized 20 µL cDNA reactions were diluted 1:5 with 80 µL sterile ...
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bioRxiv - Biochemistry 2024Quote: ... or 100 °C using a T100TM Thermal Cycler (BIO-RAD) without mixing ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit anti-cytochrome c (AHP2302, Bio-Rad Laboratories, 1:250), rabbit anti-High mobility group box 1 (HMGB1 ...
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bioRxiv - Molecular Biology 2021Quote: ... The qRT-PCR was performed in a 2-step amplification with 40 cycles of 95 °C for 30 s and 55 °C for 30s using a Bio-Rad IQ thermocycler (Bio-Rad Laboratories Inc. Hercules, CA). Each reaction was loaded on a 384-wells standard qPCR plate and ran in triplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... The enzymes were subsequently incubated at temperatures from 30 °C to 54 °C for one hour using a PCR thermocycler machine (Bio-Rad Laboratories, Hercules, CA, USA), followed by cooling for 10 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked with 3% nonfat dry milk (BioRad 1706404) in 1X PBST for 30 min at room temperature and incubated with the GAPDH antibody (Cell Signaling Technology 2118 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 μL of DNAse (1:3 in DNase buffer) (Biorad, 10042051) was added to 700 ng of RNA sample ...
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bioRxiv - Developmental Biology 2021Quote: ... on a Chromo 4 instrument running Opticon 3 software (Bio-rad). All reactions were run in triplicate and at least 3 independent RNA preparations were analysed for each sample.